Download or read book Sequence Specific Reactivity of Short Peptides written by Gary L. Juskowiak II and published by Gary L. Juskowiak II. This book was released on 2009-05-27 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Sequence specific Reactivity of Short Peptides written by Gary Lee Juskowiak (II.) and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation covers the development of combinatorial peptide library methodology to screen for short peptides with enhanced and sequence-specific reactivity; the design, synthesis, and screening of small molecule fluorescent probes for short peptide tags; and the identification of peptides with enhanced and sequence-specific reactivity. Chapter 1 covers short peptides identified from a one-bead one-compound (OBOC) library that become fluorescent under photooxidative conditions. Only one peptide (AKPWGGDA) reproduced its fluorogenic reactivity in solution phase and the fluorescent photoproduct of this peptide was identified as a 4-aminoquinoline-2-carboxylic acid, which was presumably derived from tryptophan. Chapter 2 reviews the literature on peptide tags used for fluorescently labeling proteins in living cells with a specific emphasis on the biarsenical tetracysteine system. Chapter 3 reviews the literature on the sequence-specific structure and reactivity of short peptides. It also presents elements that may be applicable to enhanced and sequence-specific reactivity between short peptide tags and small molecule probes. Chapter 4 covers OBOC and SPOT peptide library methodology developed in order to screen for the sequence-specific reactivity of short peptides with small molecule fluorescent probes. These methodological advancements were primarily in the synthesis, screening, and tandem mass spectrometry sequencing of OBOC peptide libraries, and, in particular, cysteine-rich OBOC libraries. Chapter 5 covers the design, synthesis, and screening of small molecule fluorescent probes that contained the dansyl fluorophore and esters that target lysine residues in short peptide tags. Short peptides were discovered that exhibited enhanced and sequence-specific reactivity with these fluorescent probes, and, in particular, with a tetrahydroisoquinoline N-hydroxysuccinimide ester probe in an OBOC library, in a SPOT library, and in solution phase. However, the sequence-specific reactivity of the short peptide tags is reduced outside of the bead environment. Chapter 6 covers the design, synthesis, and screening of small molecule fluorogenic probes. These fluorogenic probes consisted of FlAsH, amino coumarin alkyl esters, and benzoyl quinoline carboxyaldehydes. Short peptides were discovered that exhibited enhanced and sequence-specific reactivity for FlAsH and the amino coumarin alkyl ester probes.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Peptide Chemistry written by Miklos Bodanszky and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt: A continued interest in Peptide Chemistry prompted the revision of the first edition of this book. This provided an opportunity to update several details. I am grateful to colleagues who were kind enough to inform me of errors, typographical and other, they had discovered in the first edi tion. These have now been corrected, as were certain shortcomings in language and style pointed out by my daughter, Dr. Eva Bodanszky. In 1991 the excellent The Chemical Synthesis of Peptides by John Jones (Oxford University Press, 1991) appeared. It covers, in part, the same field, but is different enough from Peptide Chemistry, to justify publication of a revised edition of the latter. Princeton, July 1993 M. Bodanszky Preface to the First Edition Nature applied peptides for a great variety of specific functions. The specificity provided by the individual character of each amino acid is further ehanced by the combination of several amino acids into larger molecules. Peptides therefore can act as chemical messengers, neuro transmitters, as highly specific stimulators and inhibitors, regulating var ious life-processes. Entire classes of biologically active compounds, such as the opioid peptides or the gastrointestinal hormones emerged within short periods of time and it is unlikely that the rapid succession of discoveries of important new peptides would come to a sudden halt. In fact, our knowledge of the field is probably still in an early stage of development. Peptides also gained importance in our everyday life.
Download or read book The World of Peptides written by Theodor Wieland and published by Springer. This book was released on 1991 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Almost two centuries ago proteins were recognized as the primary materials (proteios = primary) oflife, but the significance and wide role of peptides (from pepsis = digestion) in practically all life pro cesses has only become apparent in the last few decades. Biologi cally active peptides are now being discovered at rapid intervals in the brain and in other organs including the heart, in the skin of amphibians and many other tissues. Peptides and peptide-like compounds are found among toxins and antibiotics. It is unlikely that this process, an almost explosive broadening of the field, will come to a sudden halt. By now it is obvious that Nature has used the combination of a small to moderate number of amino acids to generate a great variety of agonists with specific and often highly sophisticated functions. Thus, peptide chemistry must be regarded as a discipline in its own right, a major branch of biochemistry, fairly separate from the chemistry of proteins. Because of the important role played by synthesis both in the study and in the practical preparation of peptides, their area can be considered as belonging to bio-organic chemistry as well. The already overwhelming and still increasing body of know ledge renders an account of the history of peptide chemistry more and more difficult. It appears therefore timely to look back, to take stock and to recall the important stages in the development of a new discipline.
Download or read book Solid Phase Peptide Synthesis written by Gregg B. Fields and published by Academic Press. This book was released on 1997-10-21 with total page 828 pages. Available in PDF, EPUB and Kindle. Book excerpt: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volumehas been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 275 volumes have been published (all of them still in print) and much of the material is relevant even today-truly an essential publication for researchers in all fields of life sciences. Key Features * Solid-phase peptide synthesis * Applications of peptides for structural and biological studies * Characterization of synthetic peptides
Download or read book MHC Ligands and Peptide Motifs written by Hans-Georg Rammensee and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 467 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is centered on a comprehensive list of MHC peptide motifs and ligands as known to date, together with selected T cell epitopes, arranged in an easy-to-read fashion. This information is put into context by chapters on MHC gene organization, MHC structure, T cell epitope prediction, antigen processing and T cell responses. In addition, the book provides a great deal of complementary information: amino acid sequences of MHC class I alpha1 and alpha2 domains and of class II alpha1 and beta1 domains, the established or predicted composition and specificity of MHC pockets, notes on MHC nomenclature including old assignments and reference to useful internet addresses. A handy reference manual that should be helpful for all those dealing with MHC-associated peptides.
Download or read book Optimization of Protein Bioconjugation Reactions Using Combinatorial Peptide Libraries written by Leah Witus and published by . This book was released on 2012 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: The covalent attachment of chemical groups to proteins is an important tool for many areas of chemical biology, including the study of protein function and the creation of protein-based materials. In order to create well-defined protein bioconjugates, methods of site-specfic protein modification are required. We have developed a high-throughput method of optimizing protein bioconjugation reactions using a combinatorial peptide library in which short peptides serve as a model for protein reactivity. The library screening was achieved using a one-bead-one-compound peptide library, a colorimetric detection scheme to identify high-yielding sequences, and a deconvolution method using a built-in peptide truncation ladder for rapid mass spectrometry sequencing of hit beads. We applied this screening platform in the context of a pyridoxal 5'-phosphate (PLP)-mediated transamination reaction specific for the N-terminus, and were able to identify an N-terminal sequence with high reactivity towards this reaction that can be incorporated into proteins of interest to result in high levels of modification. The screening platform was also used for the discovery and optimization of new N-terminal protein modification reactions.
Download or read book Peptide Antibodies written by Gunnar Houen and published by Humana. This book was released on 2015-10-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This extensive volume covers basic and advanced aspects of peptide antibody production, characterization and uses. Although peptide antibodies have been available for many years, they continue to be a field of active research and method development. For example, peptide antibodies which are dependent on specific posttranslational modifications are of great interest, such as phosphorylation, citrullination and others, while different forms of recombinant peptide antibodies are gaining interest, notably nanobodies, single chain antibodies, TCR-like antibodies, among others. Within this volume, those areas are covered, as well as several technical and scientific advances: solid phase peptide synthesis, peptide carrier conjugation and immunization, genomics, transcriptomics, proteomics and elucidation of the molecular basis of antigen presentation and recognition by dendritic cells, macrophages, B cells and T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Peptide Antibodies: Methods and Protocols serves as an ideal reference for researchers exploring this vital and expansive area of study.
Download or read book Chemical Ligation written by Luca D. D'Andrea and published by John Wiley & Sons. This book was released on 2017-04-03 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presenting a wide array of information on chemical ligation – one of the more powerful tools for protein and peptide synthesis – this book helps readers understand key methodologies and applications that protein therapeutic synthesis, drug discovery, and molecular imaging. • Moves from fundamental to applied aspects, so that novice readers can follow the entire book and apply these reactions in the lab • Presents a wide array of information on chemical ligation reactions, otherwise scattered across the literature, into one source • Features comprehensive and multidisciplinary coverage that goes from basics to advanced topics • Helps researchers choose the right chemical ligation technique for their needs
Download or read book Gerontological Aspects of Genome Peptide Regulation written by Vladimir Khat?skelevich Khavinson and published by Karger Medical and Scientific Publishers. This book was released on 2005-01-01 with total page 115 pages. Available in PDF, EPUB and Kindle. Book excerpt: This monograph narrates not only the theoretical aspects and experimental data, but also outlines new approaches to the prevention of aging and age-related pathology, thus addressing a wide readership of gerontologists, geneticists, molecular biologists, biochemists, and pharmacologists alike.
Download or read book Advances in DNA Sequence Specific Agents written by B.J. Chapman and published by Elsevier. This book was released on 2002-09-17 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt: This series encompasses design, synthesis, application, and analytical methods (including clinical and in vitro) for the study of these critical interactions. As our understanding of the genome and proteome expands, general developments in the field of DNA sequence specific interaction are likely to play an increasingly important role. Accordingly, manuscripts have been solicited from experts covering a diverse range of fields, reflecting the cross-disciplinary and dynamic nature of the series.Volume 4 describes work on the modification of DNA by AT specific anticancer drugs, DNA alkylation events which involve metabolite generation, DNA sequence recognition by two selective binders, bulged DNA microenvironments as molecular targets, DNA sequence specific binding by short peptides and the analysis of DNA-protein interactions using DNase I footprinting methodology.Features include:• Expert contributors from the Biomedical world• Emerging areas of drug design and therapeutic applications• Nucleic acid-protein interactions• Color graphics of molecular modeling analyses• New and emerging methodologies
Download or read book Bioactive Peptides written by John Howl and published by CRC Press. This book was released on 2019-08-30 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: Focuses on Biology, Pharmacology, and Therapeutic Applications The study and diverse applications of bioactive peptides traverse many sub-disciplines within chemistry, biology, physics, and medicine. Answering a long-standing need, Bioactive Peptides focuses on the biology, pharmacology, and therapeutic applications of endogenous peptide mediators and their analogues. Moving peptide science beyond chemical synthesis strategies and into the realms of peptide biology and therapeutics, it presents the overall contribution that peptide science has made to molecular, cellular, and whole organism biology, while also discussing future targets and therapeutic applications. Beneficial for Experts and Novices Alike Part I provides details of bioactive peptides that interact with common drug targets and analyzes some of the most competitive areas of current research worldwide. While it is widely known that mammalian physiological systems utilize bioactive peptides that have yet to be discovered, other animals provide a rich and valuable source of bioactive peptides. This fascinating area of science is the theme of Part II. Parts III and IV investigate the unique bioactivities of various peptides that are ripe for further exploration. This definitive reference also includes: A detailed description and analysis of a broad range of peptides that interact with G protein-coupled receptors, the quantitatively dominant drug target A discussion of non-ribosomal peptides, which hold promise as sources of endogenous mediators Important examples of common methodologies employed to identify, characterize, and further develop bioactive peptides from a range of natural sources With mounting worldwide interest in their therapeutic potential, bioactive peptides--includ
Download or read book Protein Folding Protocols written by Yawen Bai and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering experiment and theory, bioinformatics approaches, and state-of-the-art simulation protocols for better sampling of the conformational space, this volume describes a broad range of techniques to study, predict, and analyze the protein folding process. Protein Folding Protocols also provides sample approaches toward the prediction of protein structure starting from the amino acid sequence, in the absence of overall homologous sequences.
Download or read book Immunobiology of Proteins and Peptides VI written by M. Zouhair Atassi and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: The articles in this volume represent papers delivered by invited speakers at the 6th International Symposium on the Immunobiology of Proteins and Peptides. In addition, a few of the abstracts submitted by participants were scheduled for minisymposia and some of the authors, whose presentations were judged by the Scientific Council to be of high quality, were invited to submit papers for publication in this volume. This symposium was established in 1976 for the purpose of bringing together, once every two or three years, active investigators in the forefront of contemporary immunology, to present their findings and discuss t heir significance in the light of current concepts and to identify important new directions of investigation. The founding of the symposium was stimulated by the achievement of major breakthroughs in the understanding of the immune recognition of proteins and peptides. We believed that these breakthroughs will lead to the creation of a new generation of peptide reagents which should have enormous potential in biological, therapeutic and basic applications. This anticipated explosion has in fact since occurred and many applications of these pep tides are now being realized.
Download or read book Immunohistochemistry written by Simon Renshaw and published by Scion Publishing Ltd. This book was released on 2006-12-20 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: 1. Introduction (S Renshaw) 2. Antibodies for immunochemistry (C Onley) 3. The selection of reporter labels (S Mardle) 4. Immunochemical staining techniques (S Renshaw) 5. Multiple immunochemical staining techniques (I Jones) 6. Confocal microscopy and immunohistochemistry (M Cuttle) 7. Ultrastructural immunochemistry (J Skepper) 8. Image capture, analysis and quantification (D Tannahill) 9. Quality assurance in immunohistochemistry (P Jackson) 10. Automated immunochemistry (E Schenck) List of suppliers; Index
Download or read book Protein Peptide Letters written by and published by . This book was released on 1996-10 with total page 70 pages. Available in PDF, EPUB and Kindle. Book excerpt:
