Download or read book Separation of Plant Cells Using Ficoll Gradients written by Johanna Galindo and published by . This book was released on 2019 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants are an important source for different pharmaceutical molecules. This often results in the destruction of certain plants. Plant cell culture is a technique that allows the production of molecules from a small explant rather than the destruction of naturally occurring habitats and ecosystems. Plan callus cell populations have the potential for viable production of secondary metabolites if we are able to select highly productive cell lines which produce the specific molecule of interest. Fractionation by density and sixe of the individual cell populations through Ficoll density gradient centrifugation and separation can be the solution for maintaining cell lines that produce a specific secondary metabolite. ... This study compares diffferent Ficoll density gradients to successfully separate plant cell lines or plant cell aggregates from four different types of plants including Beta vulgarius, Daucus carota, Nicotiana tabacum, and Taxus spp. These plant cell suspension cultures could potentially be used to produce more secondary metabolites.
Download or read book The Impact of Food Bioactives on Health written by Kitty Verhoeckx and published by Springer. This book was released on 2015-04-29 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: “Infogest” (Improving Health Properties of Food by Sharing our Knowledge on the Digestive Process) is an EU COST action/network in the domain of Food and Agriculture that will last for 4 years from April 4, 2011. Infogest aims at building an open international network of institutes undertaking multidisciplinary basic research on food digestion gathering scientists from different origins (food scientists, gut physiologists, nutritionists...). The network gathers 70 partners from academia, corresponding to a total of 29 countries. The three main scientific goals are: Identify the beneficial food components released in the gut during digestion; Support the effect of beneficial food components on human health; Promote harmonization of currently used digestion models Infogest meetings highlighted the need for a publication that would provide researchers with an insight into the advantages and disadvantages associated with the use of respective in vitro and ex vivo assays to evaluate the effects of foods and food bioactives on health. Such assays are particularly important in situations where a large number of foods/bioactives need to be screened rapidly and in a cost effective manner in order to ultimately identify lead foods/bioactives that can be the subject of in vivo assays. The book is an asset to researchers wishing to study the health benefits of their foods and food bioactives of interest and highlights which in vitro/ex vivo assays are of greatest relevance to their goals, what sort of outputs/data can be generated and, as noted above, highlight the strengths and weaknesses of the various assays. It is also an important resource for undergraduate students in the ‘food and health’ arena.
Download or read book Centrifugal Separations in Molecular and Cell Biology written by G.D. Birnie and published by Butterworth-Heinemann. This book was released on 2014-06-28 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: Centrifugal Separations in Molecular and Cell Biology focuses on the application of modern centrifugation technology in molecular and cell biology, including the separation and fractionation of biological particles by centrifugation on the preparative and analytical scales. The selection first covers the principles and practices of centrifugation and the bases of centrifugal separations. Discussions focus on the basic concepts of sedimentation theory, centrifugation methods, designing centrifugation experiments, care of centrifuges and rotors, and statistical estimation of molecular parameters. The book also ponders on the practical aspects of rate-zonal centrifugation, including gradient materials, density and viscosity of glycerol solutions, and resolution and gradient shape. The publication examines fractionations in zonal rotors and the quantitative aspects of rate-zonal centrifugation. The text then reviews isopycnic centrifugation in ionic media and analytical centrifugation. Topics include separation by isopycnic banding, large-scale preparative procedures, and density-gradient solutes. The selection is a valuable reference for readers interested in centrifugation technology.
Download or read book Centrifugation in Density Gradients written by C. A. Price and published by Academic Press. This book was released on 2014-06-28 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: Centrifugation in Density Gradients provides information pertinent to the fundamental aspects of density gradient centrifugation. This book discusses the benefits of density gradient centrifugation to membrane-bound particles. Organized into nine chapters, this book begins with an overview of the method of differential or fractional centrifugation. This text then explores the physical basis of density gradient centrifugation. Other chapters deal with the nuts and bolts of density gradient centrifugation, the construction and composition of gradients, the properties and operation of centrifuge systems, and certain arcane but highly useful procedures. This book discusses as well density gradient centrifugation in the analytical ultracentrifuge. The final chapter deals with a collection of protocols for separating particles ranging in size from whole cells to macromolecules. This book is intended to be suitable for readers who need to separate biological particles. Biologists, chemists, biochemists, cytologists, physiologists, scientists, and research workers will also find this book useful.
Download or read book Density Gradient Centrifugation written by and published by Elsevier. This book was released on 1978-01-01 with total page 543 pages. Available in PDF, EPUB and Kindle. Book excerpt: Density Gradient Centrifugation
Download or read book Cell Separation written by Thomas G. Pretlow and published by Academic Press. This book was released on 2014-05-10 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Separation: Methods and Selected Applications is a compendium of articles on the design and/or application of methods for the separation of cells. This volume presents contributions on relatively finite subjects on cell separation. It covers topics on cell separation such as methods for obtaining cells in suspension from animal tissues; some of the kinds of data that are helpful in the description of cell purifications; and separation of host cells infiltrating tumors and allografts by velocity sedimentation at unit gravity. The separation of different kinds of nucleated cells from blood by centrifugal elutriation; a new approach to the separation of cells at unit gravity; and the isolation and culture of homogeneous populations of glomerular cell types are elucidated as well. Experimental oncologists, hematologists, immunologists, cell biologists, endocrinologists, and others who are not already expert in the use of methods for cell separation will find the book highly useful.
Download or read book The Molecular Biology of Plant Cells written by H. Smith and published by Univ of California Press. This book was released on 1977-01-01 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant cell structure and function; Gene expression and its regulation in plant cells; The manipulation of plant cells.
Download or read book Plant Cell Vacuoles written by Deepesh De and published by CSIRO PUBLISHING. This book was released on 2000-03-01 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the only comprehensive work, at introductory level, on plant cell vacuoles. Vacuoles are ubiquitous, multifaceted and indispensable organelles and yet they have been thinly treated in the literature to date. This is at odds with the amount of interest in vacuoles that has been expressed in the last two decades. This comprehensive work provides a solid foundation on vacuoles to an advanced level. The latest research findings have been included in all aspects of plant and yeast vacuoles. The book synthesizes all the available information on the plant cell vacuole. It includes methodologies, occurrence and diversity, structure and biochemistry of tonoplasts and molecular biology of biogenesis and diverse functions, all presented in a concise way. The tremendous surge in the genetic engineering of plants for commercial products requires a comprehension of the functions and possibilities of vacuole manipulation since most of the targets of improvement directly involve vacuoles. Thus the work will be valuable to students of plant sciences, plant breeding, cell biology and plant biotechnology, as well as advanced researchers who seek a better understanding of this vital organelle.
Download or read book Plant Cells and their Organelles written by William V. Dashek and published by John Wiley & Sons. This book was released on 2017-01-17 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Cells and Their Organelles provides a comprehensive overview of the structure and function of plant organelles. The text focuses on subcellular organelles while also providing relevant background on plant cells, tissues and organs. Coverage of the latest methods of light and electron microscopy and modern biochemical procedures for the isolation and identification of organelles help to provide a thorough and up-to-date companion text to the field of plant cell and subcellular biology. The book is designed as an advanced text for upper-level undergraduate and graduate students with student-friendly diagrams and clear explanations.
Download or read book Isolation of Plant Organelles and Structures written by Nicolas L. Taylor and published by Humana. This book was released on 2016-10-12 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized function in plant cells can be advanced. Written for the highly successful Methods in Molecular Biology series, expert contributors provide chapters that contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Isolation of Plant Organelles and Structures: Methods and Protocols will greatly aid those who regularly isolate subcellular components as well as those whose research has lead them to focus on a subcellular compartment or a particular process for the first time, thus producing the need to be able to isolate it or enrich it for study.
Download or read book Chromosome Techniques written by Archarna Sharma and published by CRC Press. This book was released on 2020-08-18 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual covers the study of chromosomes in plants, animal and human systems, dealing with the protocols and principles involved. It caters to the requirements of scientists working laboratories, presenting details of the operational mechanism for use at the chromosome level.
Download or read book Chromosome Techniques written by Arun Sharma and published by Butterworth-Heinemann. This book was released on 2014-04-24 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromosome Techniques: Theory and Practice, Third Edition focuses on chromosome research. The book first discusses pre-treatment and hypotonic treatment. Pre-treatment for clearing the cytoplasm and softening the tissues; separation of chromosomes and clarification of constrictions; and hypotonic treatment for chromosome spread are described. The text also explains fixation and processing, including fixing of fluids and mixtures and air-drying techniques for chromosome study. The selection also discusses methods for special materials. Study of division in embryosac mother cells; study of chromosomes from thallophytes; salivary gland, lamp brush, and pachytene chromosomes; spiral structure; and secondary constriction are explained. The text also discusses microscopy, including ordinary light microscopy, high resolution autoradiography, and light microscope autoradiography. The book discusses study of plant chromosomes from tissue culture; chromosome analysis following short- and long-term cultures in animals, including man; and chromosome analysis from malignant tissues. The text takes a look at the banding patterns of chromosomes, including banding pattern techniques, C-banding, and representative schedules for comparative study of different banding patterns. The book further describes somatic cell fusion and the chemical nature of chromosomes, proteins, and enzymes. The text is a vital source of information for readers wanting to conduct research on chromosomes.
Download or read book Flow Cytometry and Cell Sorting written by Andreas Radbruch and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS
Download or read book Progress in Plant Cellular and Molecular Biology written by H.J. Nijkamp and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 821 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants are an important source of food and of valuable products for industry, agriculture and medicine. They are unique in many aspects of metabolic processes, development and reproduction. Most of these aspects can now be studied by the modern methods and technolo gies of molecular and cellular biology. Such studies are also encouraged as to improve plant yield and quality. During the past decade research in plant sciences has demonstrated the feasibility of plant cell and tissue culture techniques as major tools in biology and agriculture. These techniques are also essential in strategies for engineering of biological systems. The proceedings of the VII International Congress on Plant Tissue and Cell Culture in Amsterdam show that in recent years an impressive progress has been achieved. The papers of the congress, with more than 2000 participants, include the full text of plenary lectures, keynote lectures and presentations of speakers who have been selected out of more than 1400 abstracts. This combination, which provides readers with reviews as well as recent findings and future developments, captures an important part of the scientific exchange during the congress. The papers in these proceedings are a reflection of the role of plant cell and tissue culture in disciplines varying from plant breeding to molecular biology. Basic as well as applied studies in a variety of plant disciplines are presented in 4 sections: (1) Genetic manipulation and propagation, (2) Morphogenesis and metabolism, (3) Secondary metabolites and (4) Biotechnology and developing countries.
Download or read book Immobilized pH Gradients Theory and Methodology written by Pier Giorgio Righetti and published by Elsevier. This book was released on 1990-02-07 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immobilized pH gradients (IPG) represent the most advanced development of isoelectric focusing (IEF). Originally developed to overcome all the problems of IEF in soluble amphoteric buffers (CA) (such as pH gradient instability, complexation with CA chemicals, unreproducibility of pH gradients, protein precipitation at the pI), it turned out to be an entirely new technique, quite different in principle and operation from conventional IEF. The book is thus meant to bring the reader up to date with this fast developing field. The book is divided into six chapters containing information on: detailed treatment of all the chemistry of the Immobiline chemicals; theory of pH gradient generation (computer simulations, tables with all the possible pH recipes); all analytical aspects of IPGs, including staining techniques, blotting etc.; two-dimensional maps, with a detailed treatise of advantages and limitations; preparative aspects of IPGs, including comparison with other preparative electrophoretic techniques; some examples of applications, including genetic and forensic analysis, blood polymorphism etc.The book is extensive and up-to-date, while also extensively covering the theory. Clearly written, with easily accessible information, the volume is a worthy asset to the Lab. Techniques series.
Download or read book Tissue Culture written by Paul F. Jr. Kruse and published by Elsevier. This book was released on 2012-12-02 with total page 897 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.
Download or read book Isolation of Membranes and Organelles from Plant Cells written by John Lloyd Hall and published by . This book was released on 1983 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: General principles of cell fractionation. Cell walls. Plasma membranes. Higher plant vacuoles and tonoplasts. Endoplasmic reticulum and ribosomes. The golgi apparatus. Mitochondria and sub-mitochondrial particles. Chloroplasts. Microbodies. Nuclei. Partition in aqueous polymer two-phase systems - a rapid method for separation of membranes particles according to their surface properties.
