Download or read book Regulated Proteolysis in Arabidopsis Thaliana written by Adam Joel Book and published by . This book was released on 2009 with total page 243 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Selective Proteolysis in Arabidopsis Thaliana written by Nicholas Patrick Gladman and published by . This book was released on 2015 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein degradation by the ubiquitin (Ub)/26S proteasome system (UPS) is an essential eukaryotic pathway that removes mis-folded/aberrant polypeptides and short-lived regulatory proteins from the cell. Proteolysis through the UPS is mediated by the post-translational modification of target proteins with a covalently bonded poly-Ub chain. Proteins tagged with Ub chains are then recognized by the 26S proteasome, an ATP-dependent protease that cleaves the target into smaller polypeptides that will then be degraded further into individual amino acid residues by other cellular proteases. The 26S proteasome is a 2.5-MDa particle composed of at least 33 unique subunits that form two subcomplexes: the 20S core protease (CP) that houses the proteolytic active sites and the 19S regulatory particle (RP) that binds to the CP and bestows a variety of functions onto the 26S holoprotease. Proteasome activity is modulated by subunit expression and composition, various post-translational modifications, associated factors, and other activating particles. My thesis is focused on understanding UPS function and regulation in response to stress in Arabidopsis thaliana. Previous small-scale transcriptomic and translational studies in eukaryotes showed that proteasome-specific stress induces a concerted transcriptional up-regulation of numerous UPS genes, especially those that encode 26S proteasome subunit genes. I have performed the first RNA-sequencing (RNAseq) analysis of Arabidopsis seedlings during conditions that challenge cellular proteasome capacity and identified a conserved suite of up-regulated genes, which I term the proteasome stress-induced regulon (PSIR). I also characterized the DNA-binding motif landscape in the upstream regions of PSIR and other UPS genes and identified a collection of dedicated cis-acting regulatory motifs that can be flexibly recognized by numerous DNA-binding proteins. Finally, I demonstrated that two plant-specific NAM-ATAF1,2-CUC2 (NAC) family transcription factors, NAC53 and NAC78, are activators of the PSIR during proteasome-specific stress. Arabidopsis seedlings overexpressing NAC78 robustly activates the regulon, whereas double mutants lacking both factors are incapable of properly up-regulating proteasome gene transcripts, resulting in a strong hypersensitivity of seedlings to proteasome inhibition. NAC53 and NAC78 are closely related over dicot evolution, and are likely part of a conserved feedback mechanism that is responsible for regulating UPS activity across multiple plant lineages.
Download or read book Regulated Proteolysis of Arabidopsis Argonaute1 written by and published by . This book was released on 2015 with total page 185 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Proteolytic Activities Associated with the UB 26S Proteasome Pathway in Arabidopsis Thaliana written by Peizhen Yang and published by . This book was released on 2005 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Plant Cell Cycle written by Dirk Inzé and published by Springer Science & Business Media. This book was released on 2011-06-27 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, the study of the plant cell cycle has become of major interest, not only to scientists working on cell division sensu strictu , but also to scientists dealing with plant hormones, development and environmental effects on growth. The book The Plant Cell Cycle is a very timely contribution to this exploding field. Outstanding contributors reviewed, not only knowledge on the most important classes of cell cycle regulators, but also summarized the various processes in which cell cycle control plays a pivotal role. The central role of the cell cycle makes this book an absolute must for plant molecular biologists.
Download or read book Plant Proteases written by Mercedes Diaz-Mendoza and published by Frontiers Media SA. This book was released on 2020-01-24 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant proteases are involved in most aspects of plant physiology and development, playing key roles in the generation of signaling molecules and as regulators of essential cellular processes such as cell division and metabolism. They take part in important pathways like protein turnover by the degradation of misfolded proteins and the ubiquitin-proteasome pathway, and they are responsible for post-translational modifications of proteins by proteolysis at highly specific sites. Proteases are also implicated in a great variety of environmentally controlled processes, including mobilization of storage proteins during seed germination, development of seedlings, senescence, programmed cell death and defense mechanisms against pests and pathogens. However, in spite of their importance, little is known about the functions and mode of actions of specific plant proteases. This Research Topic collects contributions covering diverse aspects of plant proteases research.
Download or read book The Role of the APC C Activators in Growth and Development of Arabidopsis Thaliana written by Mikhail Baloban and published by . This book was released on 2010 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ubiquitin Médiated Proteolysis (UMP) regulates many cellular processes by polyubiquitination and consequent degradation of target proteins. The cell cycle is controlled by two ubiquitin ligases :the Skp, Cullin, F-box containing (SCF) and Anaphase-Promoting Complex/ Cyclosome (APC/C) who's complementary and consecutive actions drive the cell cycle by degradation of various cell cycle regulators. This thesis is focused on the cell and developmental role of the APC/C in Arabidopsis thaliana via studying its activators that control the temporal activity and substrate specificity of the APC/C. These are CDC20 and CDH1, the latter described in plants as CCS52. Unlike most organisms, Arabidopsis possesses multiple activator isoforms : five CDC20s and three CCS52s. This work aimed at exploring the functions of the CDC20 isoforms and that CCS52A1 and CCS52A2.
Download or read book Handbook of Photosensory Receptors written by Winslow R. Briggs and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first complete resource on photosensory receptors from bacteria, plants and animals compiles the data on all known classes of photoreceptors, creating a must-have reference for students and researchers for many years to come. Among the editors are the current and a former president of the American Society for Photobiology.
Download or read book Auxin Molecular Biology written by Cathérine Perrot-Rechenmann and published by Springer Science & Business Media. This book was released on 2011-06-27 with total page 181 pages. Available in PDF, EPUB and Kindle. Book excerpt: The plant hormone auxin plays a fundamental role in the growth and development of plants. Researchers from across the globe are currently attempting to unravel the molecular mechanisms by which auxin controls such diverse processes as cell division, cell elongation, and differentiation. Research questions on auxin action are being addressed using state-of-the-art techniques that are available to cell biologists, geneticists, molecular biologists, biochemists, and physiologists. This text highlights many of the major topics that were covered in a recent workshop that was specifically focused on research into the mechanisms of auxin action. The articles in this text give a current update of the research findings on auxin biosynthesis, metabolism and transport; evolutionary patterns; auxin perception, signal transduction and physiology; auxin-regulated gene expression and protein degradation pathway in auxin responses; and cross-talk between auxin and other plant signalling pathways.
Download or read book Regulated Proteolysis in Microorganisms written by David A. Dougan and published by Springer Science & Business Media. This book was released on 2013-03-12 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains an extensive collection of critical reviews, from leading researchers in the field of regulated protein degradation. It covers the role of regulated proteolysis in a range of microorganisms (from Gram positive, Gram negative and pathogenic bacteria to Archaea and the Baker’s yeast Saccharomyces cerevisiae).
Download or read book Proteasomes The World of Regulatory Proteolysis written by Wolfgang Hilt and published by CRC Press. This book was released on 2000-10-01 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights proteasome structures and how they are related to different aspects of proteasome function. Moreover, the book reports on the functional roles these highly developed proteolytic machines play within the cell. It was a great surprise to the scientific world that proteolysis provides crucial functions in cellular regulation. The
Download or read book Arabidopsis written by Detlef Weigel and published by CSHL Press. This book was released on 2002 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: The thale cress Arabidopsis thaliana is increasingly popular among plant scientists: it is small, easy to grow, and makes flowers, and the sequence of its small and simple genome was recently completed. This is the most complete and authoritative laboratory manual to be published on this model organism and the first to deal with genomic and proteomic approaches to its biology.
Download or read book Regulation of Proteolysis and Proteome Composition in Plant Response to Environmental Stress written by Mateusz Labudda and published by Frontiers Media SA. This book was released on 2022-12-06 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Lipid Signaling Protocols written by Teun Munnik and published by Springer Nature. This book was released on 2013-05-17 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In Plant Lipid Signaling Protocols, expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied. These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals.
Download or read book The Circadian Clock written by Urs Albrecht and published by Springer Science & Business Media. This book was released on 2010-01-23 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the invitation to edit this volume, I wanted to take the opportunity to assemble reviews on different aspects of circadian clocks and rhythms. Although most c- tributions in this volume focus on mammalian circadian clocks, the historical int- duction and comparative clocks section illustrate the importance of various other organisms in deciphering the mechanisms and principles of circadian biology. Circadian rhythms have been studied for centuries, but only recently, a mole- lar understanding of this process has emerged. This has taken research on circadian clocks from mystic phenomenology to a mechanistic level; chains of molecular events can describe phenomena with remarkable accuracy. Nevertheless, current models of the functioning of circadian clocks are still rudimentary. This is not due to the faultiness of discovered mechanisms, but due to the lack of undiscovered processes involved in contributing to circadian rhythmicity. We know for example, that the general circadian mechanism is not regulated equally in all tissues of m- mals. Hence, a lot still needs to be discovered to get a full understanding of cir- dian rhythms at the systems level. In this respect, technology has advanced at high speed in the last years and provided us with data illustrating the sheer complexity of regulation of physiological processes in organisms. To handle this information, computer aided integration of the results is of utmost importance in order to d- cover novel concepts that ultimately need to be tested experimentally.
Download or read book Transcription Factor Regulatory Networks written by Etsuko Miyamoto-Sato and published by Humana. This book was released on 2014-06-14 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transcription Factor Regulatory Methods details various techniques ranging from cutting-edge to general techniques use to study transcription factor regulatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Transcription Factor Regulatory Methods aids scientists in the further study into post-genomic or the personal genomic era.
Download or read book Plant Stress Tolerance written by Ramanjulu Sunkar and published by Springer Nature. This book was released on with total page 293 pages. Available in PDF, EPUB and Kindle. Book excerpt:
