Download or read book Real Time Analysis of Biomolecular Interactions written by K. Nagata and published by Springer Science & Business Media. This book was released on 2013-12-11 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: The metabolism of all living organisms consists of elaborate and complex interactions among numbers of biomolecules, including protein-protein interactions. BIACORE is a new device that uses surface plasmon resonance for kinetic analysis of molecular interactions and automates whole analytical processes. The number of users of BIACORE is increasing worldwide, but until now nothing had been published in English to explain the principles of this new methodology and the kinds of applications it makes available. This updated and revised version of the Japanese original explains the underlying principles of BIACORE, providing concrete examples that utilize the methodology for analysis in areas ranging from basic to applied sciences. With its generous use of illustrations, this book is a valuable source of information for all users of BIACORE.
Download or read book Real time Biomolecular Interaction Analysis written by John G. Quinn and published by . This book was released on 1999 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Real time Analysis of Biomolecular Interactions written by Kazuhiro Nagata and published by Springer Verlag Tokyo. This book was released on 2000 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Surface Plasmon Resonance written by Richard B. M. Schasfoort and published by Royal Society of Chemistry. This book was released on 2017-05-30 with total page 555 pages. Available in PDF, EPUB and Kindle. Book excerpt: Surface plasmon resonance (SPR) plays a dominant role in real-time interaction sensing of biomolecular binding events, this book provides a total system description including optics, fluidics and sensor surfaces for a wide researcher audience.
Download or read book Development of Arrayed Imaging Reflectometry as a Biomolecular Interaction Analysis Platform written by Amrita Yadav and published by . This book was released on 2012 with total page 215 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The characterization of biomolecular interactions is an important step towards understanding the basic biochemical processes that govern cellular function, the aberrations that cause disease and potential therapeutic candidates. The goal of my thesis research has been to develop Arrayed Imaging Reflectometry (AIR) as a technique to characterize biomolecular interactions in terms of their binding affinity and binding kinetics. AIR is an interferometric biosensing technique that relies on the creation of a total destructive interference condition on a silicon chip coated with a thin layer of probe-functionalized silicon dioxide. Specific binding of target molecules results in an increase in the local thickness of the probe layer and a concomitant destruction of this total destructive interference condition, resulting in an increased reflectance that corresponds to the amount of bound target. Initial studies towards this goal focused on the optimization of surface functionalization chemistry for AIR experiments. Next, experiments were carried out to confirm the quantitative performance of AIR across a range of probe-analyte affinities. We combined biomolecular binding models with data obtained from spectroscopic ellipsometry and surface plasmon resonance experiments to model AIR response for two probe-analyte pairs and compared it to experimental observations. This study confirmed that AIR response is a predictable function of probe and analyte properties and thus could be used to determine binding parameters for biomolecular interactions. A modified substrate consisting of a silicon nitride/ silicon dioxide bilayer on a silicon chip was introduced to enable AIR detection under aqueous media. The thickness sensitivity of this substrate was experimentally determined to be 1 Å. We used the new substrate to measure the binding kinetics of Muscleblind-like 1 (MBNL1), a splicing regulator protein that is implicated in the pathology of the Myotonic Dystrophies and Huntington Disease, to several of its 'normal' and 'pathogenic' RNA targets microarrayed on an AIR chip. The binding kinetics of 'normal' and 'pathogenic' RNA sequences were observed to be distinctly different even though their steady state binding constants were similar. These results suggest that AIR can be a powerful technique for the label-free real-time analysis of biomolecular interactions in a high-throughput format"--Pages viii-ix.
Download or read book Electro Optically Modulated Biomolecular Interaction Analysis Sensor written by Yao Cheng and published by . This book was released on 2000 with total page 11 pages. Available in PDF, EPUB and Kindle. Book excerpt: This paper focuses on developing the platform technology of real-time biomolecular-interaction analysis (BIA) sensor chips. A detection scheme using the electro-optically modulated surface plasmon resonance (SPR) is suggested to advance the sensor features in reducing measurement complexity and time. The SPR method of a BIA sensing system detects slight changes of refractive index due to the biomolecular interaction at the solid-liquid interface. The most sensitive interrogation method among the possible conventional schemes is to measure the SPR angle of the attenuated total reflection. The electro-optical modulation replaces the mechanism of angle measurement not only to increase the speed but also to reduce the size. Recent progress of the multilayer SPR provides an effective mean of tailoring the microchip. Several multilayer configurations have been studied in this paper to realize the electro-optical SPR sensing. Especially, the long-range mode of surface plasmon was investigated to achieve the high-resolution and high-sensitivity detection.
Download or read book Handbook of Surface Plasmon Resonance written by Richard B. M. Schasfoort and published by Royal Society of Chemistry. This book was released on 2008 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: Surface plasmon resonance (SPR) plays a dominant role in real-time interaction sensing of biomolecular binding events. This book focuses on a total system description including optics, fluidics and sensor surfaces. It covers all commercial SPR systems in the market. It is the first of its kind and fills a gap in the technical literature as no other handbook on SPR is currently available. The final chapter discussed new trends and a vision is given for future developments and needs of the SPR market. This excellent handbook provides comprehensive information with easy to use, stand-alone chapte.
Download or read book Biomolecular Interaction Analysis by Surface Plasmon Resonance Combined with Mass Spectrometry written by Jonas Borch and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biomolecular Sensors written by Electra Gizeli and published by CRC Press. This book was released on 2002-05-16 with total page 497 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of devices that incorporate biological assemblies is impacting analytical and biomedical research. Today, scientists can monitor vital biological interactions-such as the binding of DNA to proteins-in real time, deriving unique information necessary to understanding biochemical pathways and thus aiding the design of drugs to regulat
Download or read book Biomolecular Interactions Part B written by and published by Academic Press. This book was released on 2022-05-25 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomolecular Interactions: Part A, Volume 169, the latest release in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including Emerging Mechanisms of Targeted Protein Degradation by Molecular Glues, Design and use of programmable DNA Hydrogels, Oligomerization of membrane receptors: Approaches to measure in live cells, Interactions of alpha-synuclein with biomolecules, Gel-electrophoresis based method for biomolecular interaction, Recombinant centrosome expression in bacterial system, Reconstituting CCL5-CCR5 complex for structural and mechanistic analysis, Protein engineering and design in ion channel receptors, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Cell Biology series - Updated release includes the latest information on biomolecular interactions instead of protein-protein interactions
Download or read book Phase Sensitive Spectral Domain Interferometry for Label Free Biomolecular Interaction Analysis and Biosensing Applications written by Sajal Chirvi and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomolecular interaction analysis (BIA) plays vital role in wide variety of fields, which include biomedical research, pharmaceutical industry, medical diagnostics, and biotechnology industry. Study and quantification of interactions between natural biomolecules (proteins, enzymes, DNA) and artificially synthesized molecules (drugs) is routinely done using various labeled and label-free BIA techniques. Labeled BIA (Chemiluminescence, Fluorescence, Radioactive) techniques suffer from steric hindrance of labels on interaction site, difficulty of attaching labels to molecules, higher cost and time of assay development. Label free techniques with real time detection capabilities have demonstrated advantages over traditional labeled techniques. The gold standard for label free BIA is surface Plasmon resonance (SPR) that detects and quantifies the changes in refractive index of the ligand-analyte complex molecule with high sensitivity. Although SPR is a highly sensitive BIA technique, it requires custom-made sensor chips and is not well suited for highly multiplexed BIA required in high throughput applications. Moreover implementation of SPR on various biosensing platforms is limited. In this research work spectral domain phase sensitive interferometry (SD-PSI) has been developed for label-free BIA and biosensing applications to address limitations of SPR and other label free techniques. One distinct advantage of SD-PSI compared to other label-free techniques is that it does not require use of custom fabricated biosensor substrates. Laboratory grade, off-the-shelf glass or plastic substrates of suitable thickness with proper surface functionalization are used as biosensor chips. SD-PSI is tested on four separate BIA and biosensing platforms, which include multi-well plate, flow cell, fiber probe with integrated optics and fiber tip biosensor. Sensitivity of 33 ng/ml for anti-IgG is achieved using multi-well platform. Principle of coherence multiplexing for multi-channel label-free biosensing applications is introduced. Simultaneous interrogation of multiple biosensors is achievable with a single spectral domain phase sensitive interferometer by coding the individual sensograms in coherence-multiplexed channels. Experimental results demonstrating multiplexed quantitative biomolecular interaction analysis of antibodies binding to antigen coated functionalized biosensor chip surfaces on different platforms are presented.
Download or read book Cellular and Biomolecular Recognition written by Raz Jelinek and published by John Wiley & Sons. This book was released on 2009-07-10 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: With its exploration of the scientific and technological characteristics of systems exploiting molecular recognition between synthetic materials, such as polymers and nanoparticles, and biological entities, this is a truly multidisciplinary book bridging chemistry, life sciences, pharmacology and medicine. The authors introduce innovative biomimetic chemical assemblies which constitute platforms for recruitment of cellular components or biological molecules, while also focusing on physical, chemical, and biological aspects of biomolecular recognition. The diverse applications covered include biosensors, cell adhesion, synthetic receptors, cell patterning, bioactive nanoparticles, and drug design.
Download or read book Molecular Diagnosis of Infectious Diseases written by Jochen Decker and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt: Populations of the western world are now healthier and enjoying higher life expectancy than ever. They are beginning to benefit from an array of costly new therapies made possible through recent rapid advances in medical science and technology, and their demands on modern medicine are rising. Meanwhile, healthcare systems are struggling with their outdated legacy models of the m- th 20 century and are experiencing ever-increasing financial pressure from g- ernments and health insurance organizations. The equation is no longer in balance, and this predicament is forcing societies to explore new approaches to managing healthcare in the future. Since the first edition of Molecular Diagnosis of Infectious Diseases was published, we have witnessed the sequencing of the (almost) complete human genome and a shift in medical research from an emphasis on genetics to the advancement and useful application of proteomics. Bioinformatics has become the key tool for managing and analyzing the upsurge of data, and faster and more effective test methods and technologies have opened up new prospects for ind- try and academia. The tools of modern genomics and proteomics are now being utilized to specifically guide the discovery of drugs for the prevention, diagnosis, and treatment of human disease. They may also help us to find a way out of the current healthcare calamity.
Download or read book Photosystem I written by John H. Golbeck and published by Springer Science & Business Media. This book was released on 2007-05-20 with total page 744 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes recent advances made in the biophysics, biochemistry, and molecular biology of the enzyme known as Photosystem I, the light-induced plastocyanin: ferredoxin oxidoreductase. The volume provides a unique compilation of chapters that includes information highlighting controversial issues to indicate the frontiers of research and places special emphasis on methodology and practice for new researchers.
Download or read book Cell Biology written by Julio E. Celis and published by Elsevier. This book was released on 2005-11-16 with total page 630 pages. Available in PDF, EPUB and Kindle. Book excerpt: This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological problems using a variety of technologies and model systems. This thoroughly updated third edition contains 165 new articles in classical as well as rapidly emerging technologies. Topics covered include: - Cell and Tissue Culture: Associated Techniques, Viruses, Antibodies, Immunocytochemistry (Volume 1) - Organelle and Cellular Structures, Assays (Volume 2) - Imaging Techniques, Electron Microscopy, Scanning Probe and Scanning Electron Microscopy, Microdissection, Tissue Arrays, Cytogenetics and In Situ Hybridization, Genomics and Transgenic Knockouts and Knock-down Methods (Volume 3) - Transfer of Macromolecules, Expression Systems, Gene Expression Profiling (Volume 4) - Indispensable bench companion for every life science laboratory - Provides the latest information on the plethora of technologies needed to tackle complex biological problems - Includes numerous illustrations, some in full color, supporting steps and results
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1996 with total page 1344 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biochemistry and Molecular Biology of Plant Hormones written by P.J.J. Hooykaas and published by Elsevier. This book was released on 1999-05-13 with total page 565 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides up-to-date coverage at an advanced level of a range of topics in the biochemistry and molecular biology of plant hormones, with particular emphasis on biosynthesis, metabolism and mechanisms of action. Each contribution is written by acknowledged experts in the field, providing definitive coverage of the field. No other modern book covers this subject matter at such an advanced level so comprehensively. It will be invaluable to university libraries and scientists in the plant biotechnology industries.
