Download or read book Rapid Freezing Freeze Fracture and Deep Etching written by Nicholas J. Severs and published by Wiley-Liss. This book was released on 1995-08-11 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: The techniques of freeze fracture and deep etching—techniques for the examination of the ultrastructure of rapidly frozen biological specimens by transmission electron microscopy—have profoundly influenced our understanding of the functional organization of the cell. Rapid Freezing, Freeze Fracture, and Deep Etching guides the reader through the principles of these techniques and gives detailed examples of their application in biomedical research. The book is organized into three sections. The first covers the fundamentals of freeze fracture and deep etching, with a discussion of basic principles for those new to the field, and an entire chapter on the equipment currently available for freeze fracture. Advanced techniques are presented next, with each chapter devoted to an in-depth treatment of a specific topic, including the advantages and limitations of each technique. The final chapters offer a series of applications selected to illustrate the wide-ranging and enduring impact of rapid freezing, freeze fracture, and deep etching on the field of biomedical research. With over 150 illustrations and containing contributions by leading experts instrumental in the development of these techniques, Rapid Freezing, Freeze Fracture, and Deep Etching offers authoritative coverage of such novel topics as: Hyperbaric freezing Ultrarapid freezing techniques in the study of dynamic cellular processes Cytochemical methods utilizing lectins and antibodies for labeling specific cellcomponents Fracturing and shadowing under ultra-low temperature and ultra-high vacuum conditions Freeze fracture and deep etching in neurobiology Rapid Freezing, Freeze Fracture, and Deep Etching will appeal to both newcomers to the field and experienced researchers and will be essential reading for cell biologists and anatomists, as well as any researcher who uses electron microscopy.
Download or read book Cryotechniques in Biological Electron Microscopy written by Rudolf A. Steinbrecht and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.
Download or read book Biomedical Electron Microscopy written by Arvid B. Maunsbach and published by Elsevier. This book was released on 1998-11-03 with total page 569 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. - Authored by the key leaders in the biological electron microscopy field - Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines - Introduces students on how to read and interpret electron micrographs
Download or read book Ultrastructure Techniques for Microorganisms written by H. C. Aldrich and published by . This book was released on 1986-08-31 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Experimental Cell Biology of Taste and Olfaction written by Andrew I. Spielman and published by CRC Press. This book was released on 1995-07-12 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experimental Cell Biology of Taste and Olfaction examines and adapts methods from a variety of established fields, such as neurophysiology, receptor biochemistry and cellular imaging to provide comprehensive coverage of current techniques and protocols in chemosensory cell biology. Written for both newcomers and established scientists, this volume offers numerous tips for problem solving and suggests ways to avoid the most common, and costly, mistakes made by researchers. This book covers general aspects such as tissue collection and preparation, as well as specific, up-to-date methods used in taste and olfactory morphology, immunology, biochemistry, biophysics, electrophysiology and molecular biology. The explosion of knowledge and the increased interest in these areas make this book an important reference work for all scientists, students, and teachers in this and related fields
Download or read book Bioimaging written by Douglas E. Chandler and published by Jones & Bartlett Publishers. This book was released on 2009 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Development Of Microscopy Revolutionized The World Of Cell And Molecular Biology As We Once Knew It And Will Continue To Play An Important Role In Future Discoveries. Bioimaging: Current Concepts In Light And Electron Microscopy Is The Optimal Text For Any Undergraduate Or Graduate Bioimaging Course, And Will Serve As An Important Reference Tool For The Research Scientist. This Unique Text Covers, In Great Depth, Both Light And Electron Microscopy, As Well As Other Structure And Imaging Techniques Like X-Ray Crystallography And Atomic Force Microscopy. Written In A User-Friendly Style And Covering A Broad Range Of Topics, Bioimaging Describes The State-Of-The-Art Technologies That Have Powered The Field To The Forefront Of Cellular And Molecular Biological Research.
Download or read book Methods in Plant Cell Biology written by David W. Galbraith and published by Academic Press. This book was released on 1995 with total page 605 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. Key Features * Written by experts, many of whom have developed the individual methods described * Contains most, if not all, the methods needed for modern research in plant cell biology * Up-to-date and comprehensive * Full references * Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures * Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants.
Download or read book Methods in Plant Cell Biology Part A written by and published by Elsevier. This book was released on 1995-10-10 with total page 605 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. Key Features* Written by experts, many of whom have developed the individual methods described* Contains most, if not all, the methods needed for modern research in plant cell biology* Up-to-date and comprehensive* Full references* Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures* Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants
Download or read book Cell Biology written by Julio E. Celis and published by Elsevier. This book was released on 2005-11-16 with total page 630 pages. Available in PDF, EPUB and Kindle. Book excerpt: This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological problems using a variety of technologies and model systems. This thoroughly updated third edition contains 165 new articles in classical as well as rapidly emerging technologies. Topics covered include: - Cell and Tissue Culture: Associated Techniques, Viruses, Antibodies, Immunocytochemistry (Volume 1) - Organelle and Cellular Structures, Assays (Volume 2) - Imaging Techniques, Electron Microscopy, Scanning Probe and Scanning Electron Microscopy, Microdissection, Tissue Arrays, Cytogenetics and In Situ Hybridization, Genomics and Transgenic Knockouts and Knock-down Methods (Volume 3) - Transfer of Macromolecules, Expression Systems, Gene Expression Profiling (Volume 4) - Indispensable bench companion for every life science laboratory - Provides the latest information on the plethora of technologies needed to tackle complex biological problems - Includes numerous illustrations, some in full color, supporting steps and results
Download or read book The Dictionary of Cell Molecular Biology written by John M. Lackie and published by Academic Press. This book was released on 2007-10-04 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: The over 10,000 entries in this comprehensive Dictionary of Cell and Molecular Biology provide clear and concise definitions for anyone working in life sciences today. It incorporates related terms from neuroscience, genetics, microbiology, immunology, pathology, and physiology. This fourth revised edition reflects the enormous changes brought about by the explosion of new technologies, especially high throughput approaches and functional genomics. As a result, this edition is over 30% larger than the previous edition, with 3400 new entries. As with the prior edition, additions are reflective of online search queries performed by users of the dictionary. The entries in this authoritative work have been widely praised for their clarity, brevity, and accuracy throughout. The Dictionary of Cell and Molecular Biology features numerous tables and other useful features.* Thoroughly revised and expanded by over 30% with 3400 new entries* Expanded coverage of areas greatly impacted by genomics* Includes new terms that relate to the recent elucidation of underlying mechanisms of cell cycle regulation, apoptosis, relationship between mitochondria and disease, metabolic control, and stem cell biology* Consistently provides the most complete short definitions of technical terminology for anyone working in life sciences today* Extensively cross-referenced* Provides multiple definitions, notes on word origins, and other useful features
Download or read book Imaging Mass Spectrometry written by Mitsutoshi Setou and published by Springer Science & Business Media. This book was released on 2010-01-29 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: Addressing the widespread need for a practical guide to imaging mass spectrometry (IMS), this book presents the protocols of IMS technology. As that technology expands, research groups around the world continue its development. Pharmaceutical companies are using IMS for drug analyses to study pharmacokinetics and medical properties of drugs. Drug research and disease-related biomarker screening are experiencing greater use of this technology, with a concurrent increase in the number of researchers in academia and industry interested in wider applications of IMS. Intended for beginners or those with limited experience with IMS technology, this book provides practical details and instructions needed for immediate know-how, including the preparation of animal tissue samples, the application of a matrix, instrumental operations, and data analysis, among others. By describing the foundations of IMS, this volume contributes to the ongoing development of the field and to progress in human health.
Download or read book ToF SIMS written by J. C. Vickerman and published by IM Publications. This book was released on 2013 with total page 742 pages. Available in PDF, EPUB and Kindle. Book excerpt: Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is the most versatile of the surface analysis techniques that have been developed during the last 30 years. This is the Second Edition of the first book ToF-SIMS: Surface analysis by Mass Spectrometry to be dedicated to the subject and the treatment is comprehensive
Download or read book Development of the Inner Ear written by Matthew Kelley and published by Springer Science & Business Media. This book was released on 2006-03-04 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Springer Handbook of Auditory Research presents a series of compreh- sive and synthetic reviews of the fundamental topics in modern auditory - search. The volumes are aimed at all individuals with interests in hearing research including advanced graduate students, postdoctoral researchers, and clinical investigators. The volumes are intended to introduce new investigators to important aspects of hearing science and to help established investigators to betterunderstandthefundamentaltheoriesanddatain?eldsofhearingthatthey may not normally follow closely. Each volume presents a particular topic comprehensively, and each servesas a synthetic overview and guide to the literature. As such, the chapters present neither exhaustive data reviews nor original research that has not yet appeared in peer-reviewed journals. The volumes focus on topics that have developed a solid data and conceptual foundation rather than on those for which a literature is only beginning to develop. New research areas will be covered on a timely basis in the series as they begin to mature. Eachvolumeintheseriesconsistsofafewsubstantialchaptersonaparticular topic. In some cases, the topics will be ones of traditional interest for which there is a substantial body of data and theory, such as auditory neuroanatomy (Vol. 1) and neurophysiology (Vol. 2). Other volumes in the series deal with topics that have begun to mature more recently, suchasdevelopment,plasticity, and computational models of neural processing. In many cases, the series - itorsarejoinedbyaco-editorhavingspecialexpertiseinthetopicofthevolume.
Download or read book Current Topics in Developmental Biology written by and published by Academic Press. This book was released on 1996-09-18 with total page 301 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 34 continues the series' tradition of timely review and incisive analyses of key research in developmental biology. Not only valuable to researchers at the forefront of animal and plant development, this volume is also a friendly introduction to students and professionals who are curious about current topics in cellular and molecular mechanisms in development.
Download or read book Isolated Hepatocytes Preparation Properties and Applications written by M.N. Berry and published by Elsevier. This book was released on 1991-09-30 with total page 483 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book sets out clearly and effectively the preparation and working methods of laboratory techniques involving isolated hepatocytes and will make life easier for every laboratory worker concerned with these techniques.
Download or read book Introduction to Electron Microscopy for Biologists written by and published by Academic Press. This book was released on 2008-10-22 with total page 562 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers). Basic techniques in transmission and scanning electron microscopy Detailed chapters on how to use electron microscopy when dealing with specific cellular structures, such as the nucleus, cell membrane, and cytoskeleton Discussion on electron microscopy of viruses and virus-cell interactions
Download or read book Plastid Biology written by Steven M. Theg and published by Springer. This book was released on 2014-09-19 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plastids are the sites of conversion of solar energy into the chemical energy usable to sustain life. They are also responsible for the production of the vast majority of the oxygen in the atmosphere. Through these activities they play a unique role in the biosphere, producing two critical products upon which life on Earth depends. It covers in 21 chapters nearly all actively investigated areas of plastid biology, from biosynthesis to function to their uses in biotechnology. The editors have compiled an extensive list of international experts from whom to solicit chapters. As is evident from the suggested Table of Contents, the book will start with a discussion of genetic material and its expression, followed by differentiation and development of different plastid types and internal organization. This is followed by an in depth look at biogenesis and assembly of plastid proteins and protein complexes and then by the important metabolic functions in plastids. The book will end with two chapters discussing the role of plastid biology in protein expression biotechnology and in hydrogen and biofuel production.
