Download or read book X ray Microanalysis in Biology written by David C. Sigee and published by Cambridge University Press. This book was released on 1993-04-22 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes an integrated approach to the use of X-ray microanalysis in biology.

Download or read book Immunogold Silver Staining written by M. A. Hayat and published by CRC Press. This book was released on 2024-11-01 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.

Download or read book Low Temperature Microscopy and Analysis written by Patrick Echlin and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 553 pages. Available in PDF, EPUB and Kindle. Book excerpt: The frozen-hydrated specimen is the principal element that unifies the subject of low temperature microscopy, and frozen-hydrated specimens are what this book is all about. Freezing the sample as quickly as possible and then further preparing the specimen for microscopy or microanalysis, whether still embedded in ice or not: there seem to be as many variations on this theme as there are creative scientists with problems of structure and composition to investigate. Yet all share a body of com mon fact and theory upon which their work must be based. Low-Temperature Micros copy and Analysis provides, for the first time, a comprehensive treatment of all the elements to which one needs access. What is the appeal behind the use of frozen-hydrated specimens for biological electron microscopy, and why is it so important that such a book should now have been written? If one cannot observe dynamic events as they are in progress, rapid specimen freezing at least offers the possibility to trap structures, organelles, macro molecules, or ions and other solutes in a form that is identical to what the native structure was like at the moment of trapping. The pursuit of this ideal becomes all the more necessary in electron microscopy because of the enormous increase in resolution that is available with electron-optical instruments, compared to light optical microscopes.

Download or read book Principles and Practice of Fertility Preservation written by Jacques Donnez and published by Cambridge University Press. This book was released on 2011-02-03 with total page 569 pages. Available in PDF, EPUB and Kindle. Book excerpt: The specialty of fertility preservation offers patients with cancer, who are rendered infertile by chemo- and radiotherapy, the opportunity to realize their reproductive potential. This gold-standard publication defines the specialty. The full range of techniques and scientific concepts is covered in detail, and the author team includes many of the world's leading experts in the field. The book opens with introductions to fertility preservation in both cancer and non-cancer patients, followed by cancer biology, epidemiology and treatment, and reproductive biology and cryobiology. Subsequent sections cover fertility preservation strategies in males and females, including medical/surgical procedures, ART, cryopreservation and transplantation of both ovarian tissue and the whole ovary, and in-vitro follicle growth and maturation. Concluding chapters address future technologies, as well as ethical, legal and religious issues. Richly illustrated throughout, this is a key resource for all clinicians specializing in reproductive medicine, gynecology, oncology, hematology, endocrinology and infertility.

Download or read book Vitrification in Assisted Reproduction written by Michael Tucker and published by CRC Press. This book was released on 2007-11-01 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cryopreservation methods have become well established as an increasingly important therapeutic strategy in assisted reproduction. Vitrification in Assisted Reproduction: A User's Manual and Trouble-shooting Guide addresses this cryopreservation technique from an academic and technical viewpoint, with specific reference to assisted reproduction in h

Download or read book Techniques in Immunocytochemistry written by and published by Academic Press. This book was released on 2012-12-02 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: Once again, the text is fully illustrated with high quality color and black-and-white photographs, and carefully organized so as to aid both the newcomer and the established researcher to extend his or her field of expertise. - In situ hybridization for the detection of cellular products - Low temperature techniques and their applications - Silver enhancement technology - Immunocytochemistry of excitatory amino acids in the brain

Download or read book Physical Methods in Plant Sciences written by Hans-Ferdinand Linskens and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 301 pages. Available in PDF, EPUB and Kindle. Book excerpt: Latest techniques for the analysis of plant cell or tissue structure and the registration of physiological pathways are topics of this volume. The subjects include: - Laser Doppler Vibrometer Measurements of Leaves; - Laster Physical Methods. Laser Microprobe Mass Spectrometry; - Triplet States in Photosynthesis: Linear Dichroic Optical Difference Spectra via Magnetic Resonance; - Fast Atom Bombardment Mass Spectrometry; -Microdissection and Biochemical Analysis of Plant Tissues; - Photoacoustic Spectroscopy - Photoacoustic and Photothermal Effects; - Membrane Operational Impedance of Spectra of Plant Cell.

Download or read book Ex Situ Plant Conservation written by Center for Plant Conservation and published by Island Press. This book was released on 2013-02-22 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: Faced with widespread and devastating loss of biodiversity in wild habitats, scientists have developed innovative strategies for studying and protecting targeted plant and animal species in "off-site" facilities such as botanic gardens and zoos. Such ex situ work is an increasingly important component of conservation and restoration efforts. Ex Situ Plant Conservation, edited by Edward O. Guerrant Jr., Kayri Havens, and Mike Maunder, is the first book to address integrated plant conservation strategies and to examine the scientific, technical, and strategic bases of the ex situ approach. The book examines where and how ex situ investment can best support in situ conservation. Ex Situ Plant Conservation outlines the role, value, and limits of ex situ conservation as well as updating best management practices for the field, and is an invaluable resource for plant conservation practitioners at botanic gardens, zoos, and other conservation organizations; students and faculty in conservation biology and related fields; managers of protected areas and other public and private lands; and policymakers and members of the international community concerned with species conservation.

Download or read book Advances in Heat Transfer written by and published by Academic Press. This book was released on 1992-08-07 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Advances in Heat Transfer begins with an excellent overview of heat transfer in bioengineering. Subsequent chapters lead the reader through fundamental approaches for analyzing the response of living cells and tissues to temperature extremes, state-of-the-art mathematical models of bioheat transfer, an extensive review of mathematical models of bioheat transfer processes at high and low temperatures, and experimental tools for temperature measurement. This volume will effectively aid any researcher in the field by illuminating a greater understanding of fundamental issues relevant to heat transfer processes in biosystems. Key Features* Presents the fundamentals and applications of heat and mass transfer in biomedical systems* Presents a review of mathematical models for bioheat transfer, including heat transfer at temperature extremes* Includes detailed discussions of state-of-the-art bioheat equations* Explains techniques for temperature measurement in the human body

Download or read book Plant Breeding Reviews Volume 23 written by Jules Janick and published by John Wiley & Sons. This book was released on 2010-04-07 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Breeding Reviews is an open-ended, serial continuation series of review articles on research in plant genetics, especially the breeding of commercially important crops. This detailed analysis bridges the gap between the specialized researcher and the broader community of plant scientists.

Download or read book Electron Microbeam Analysis written by Abraham Boekestein and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 271 pages. Available in PDF, EPUB and Kindle. Book excerpt: This supplement of Mikrochimica Acta contains selected papers from the Second Workshop of the European Microbeam Analysis Society (EMAS) "Modern Developments and Applications in Microbeam Analysis", on which took place in May 1991 in Dubrovnik (Yugoslavia). EMAS was founded in 1987 by members from almost all European countries, in order to stimulate research, applications and development of all forms of microbeam methods. One of the most important activities EMAS is the organisation of biannual workshops for demonstrating the current status and developing trends of microbeam methods. For this meeting, EMAS chose to highlight the following topics: electron-beam microanalysis (EPMA) of thin films and quantitative analysis of ultra-light elements, Auger electron spectroscopy (AES), electron energy loss spec trometry (EELS), high-resolution transmission electron microscopy (HRTEM), quantitative analysis of biological samples and standard-less electron-beam microanalysis. Seven introductory lectures and almost seventy poster presentations were given by speakers from twelve European and two non-European (U.S.A. and Argentina) countries were made. One cannot assume that all fields of research in Europe were duly represented, but a definite trend is discernible. EPMA with wavelength-dispersive spectrometry (WDS) or energy-dispersive spectrometry (EDS) is the method with by far the widest range of applications, followed by TEM with EELS and then AES. There are also interesting suggestions for the further development of new appa ratus with new fields of application. Applications are heavily biased towards materials science (thin films in microelectronics and semicon ductors), ceramics and metallurgy, followed by analysis of biological and mineral samples.

Download or read book Cryotechniques in Biological Electron Microscopy written by Rudolf A. Steinbrecht and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.

Download or read book X ray Microscopy written by Chris Jacobsen and published by Cambridge University Press. This book was released on 2019-12-19 with total page 595 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by a pioneer in the field, this text provides a complete introduction to X-ray microscopy, providing all of the technical background required to use, understand and even develop X-ray microscopes. Starting from the basics of X-ray physics and focusing optics, it goes on to cover imaging theory, tomography, chemical and elemental analysis, lensless imaging, computational methods, instrumentation, radiation damage, and cryomicroscopy, and includes a survey of recent scientific applications. Designed as a 'one-stop' text, it provides a unified notation, and shows how computational methods in different areas are linked with one another. Including numerous derivations, and illustrated with dozens of examples throughout, this is an essential text for academics and practitioners across engineering, the physical sciences and the life sciences who use X-ray microscopy to analyze their specimens, as well as those taking courses in X-ray microscopy.

Download or read book Cryopreparation of Thin Biological Specimens for Electron Microscopy written by Norbert Roos and published by Oxford University Press, USA. This book was released on 1990 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cryotechniques offer a number of advantages over conventional electron microscopy methods in biology, particularly with regard to preserving the morphology and composition of specimens. This handbook provides a practical guide for researchers, students, and technicians who wish to employ cryotechniques in their work. It covers those methods where the cryofixed specimen is sectioned or otherwise presented as a thin film for examination, giving special attention to topics of current interest. This guide will be of use to workers in a variety of biomedical disciplines, including cell biology, molecular biology, bacteriology, and pathology.

Download or read book Food Freezing written by William B. Bald and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt: Food Freezing: Today and Tomorrow represents the contributions of the invited speakers to an advanced study seminar held by the Institute for Applied Biology of the University of York during April 1990. The main objective of the seminar was to bring together food scientists and engineers to discuss their mutual problems and to seek new directions for possible solutions.

Download or read book Correlative Light and Electron MIcroscopy written by Thomas Muller-Reichert and published by Academic Press. This book was released on 2012-08-10 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods

Download or read book EUREM 88 written by Peter J. Goodhew and published by . This book was released on 1988 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: