Download or read book Pulsed Field Gel Electrophoresis written by Bruce Birren and published by Academic Press. This book was released on 2012-12-02 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pulsed Field Gel Electrophoresis: A Practical Guide is the first laboratory manual to describe the theory and practice of this technique. Based on the authors' experience developing pulsed field gel instruments and teaching procedures, this book provides everything a researcher or student needs to know in order to understand and carry out pulsed field gel experiments. Clear, well-tested protocols assume only that users have a basic familiarity with molecular biology. Thorough coverage of useful data, theory, and applications ensures that this book is also a lasting resource for more advanced practitioners of pulsed field gels. Reviews all types of pulsed field gel electrophoresis Describes all commercially available systems and summarizes advantages and limitations of each Includes step-by-step protocols for sample preparation and analysis Illustrated with photographs that depict How to run gels: What the results should look like What they look like when they go wrong Covers applications to a wide range of organisms Includes bibliography of over 900 publications and cross-referenced by topic, application, and organism

Download or read book Pulsed Field Gel Electrophoresis written by Margit Burmeister and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents step-by-step protocols for users of Pulsed-Field Gel Electrophoresis (PFGE), whether novice or expert. This book features a wide range of PFGE techniques, auxiliary methods, and a diverse array of powerful applications. It includes protocols designed to work even the first time they are used.

Download or read book Pulsed Field Gel Electrophoresis written by Anthony P. Monaco and published by IRL Press. This book was released on 1995 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pulsed field gel electrophoresis (PFGE) has provided a very reliable system for separation of DNA fragments greater than 50 kb and has made a significant impact on the analysis of both prokaryotic and eukaryotic genomes. The chapters in this book cover both the theory behind this very important technique and present detailed protocols for many of its major uses. The first chapter describes the basis of PFGE with interesting new insights in modelling how PFGE separates large fragments of DNA. The next two describe the use of PFGE in constructing long range restriction site maps in mammalian genomic DNA and in the detection of gross rearrangements in the DNA of patients with Duchenne muscular dystrophy and Charcot-Marie-Tooth disease. There are four chapters dedicated to the use of PFGE in cloning form, construction of, analysis of, and transfer of yeast artificial chromosomes (YACs). The final two chapters describe the use of PFGE in the analysis of bacterial chromosomes and protozoan parasite chromosomes. This book will therefore be an essential resource book for researchers in laboratories that require PFGE in their research strategies, including those involved in molecular human genetics, mammalian genetics, molecular microbiology, molecular parasitology, biochemistry, and molecular diagnostics.

Download or read book Pulsed field Gel Electrophoresis written by and published by . This book was released on 1990 with total page 86 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Pulse Field Gel Electrophoresis written by Kieran Jordan and published by Humana. This book was released on 2016-10-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume will be of interest to epidemiologists, food microbiologists, and anyone working on comparing bacterial isolates. Pulse Field Gel Electrophoresis: Methods and Protocols guides readers through methods and protocols that will advance the harmonisation of PFGE methodologies and facilitate inter-laboratory comparisons of PFGE profiles from pathogenic and non-pathogenic bacteria. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, Pulse Field Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Download or read book Molecular Bacteriology Protocols and Clinical Applications written by Neil Woodford and published by Humana Press. This book was released on 2010-11-09 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The enormous advances in molecular biology that have been witnessed in . Not recent years have had major impacts on many areas of the biological sciences least of these has been in the field of clinical bacteriology and infectious disease . Molecular Bacteriology: Protocols and ClinicalApplications aims to provide the reader with an insight into the role that molecular methodology has to play in modern medical bacteriology. The introductory chapter ofMolecular Bacteriology: ProtocolsandCli- cal Applications offers a personal overview by a Consultant Medical Microbio- gist of the impact and future potential offered by molecular methods. The next six chapters comprise detailed protocols for a range of such methods . We believe that the use of these protocols should allow the reader to establish the various methods described in his or her own laboratory. In selecting the methods to be included in this section, we have concentrated on those that, arguably, have greatest current relevance to reference clinical bacteriology laboratories; we have deliberately chosen not to give detailed protocols for certain methods, such as multilocus enzyme electrophoresis that, in our opinion, remain the preserve of specialist la- ratories and that are not currently suited for general use. We feel that the methods included in this section will find increasing use in diagnostic laboratories and that it is important that the concepts, advantages, and limitations of each are th- oughly understood by a wide range of workers in the field .

Download or read book Pet to Man Travelling Staphylococci written by Vincenzo Savini and published by Academic Press. This book was released on 2018-03-14 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pet-to-Man Travelling Staphylococci: A World in Progress explores Staphylococci, a dangerous pathogen that affects both humans and animals with a wide range of infection states. This bacteria can spread rapidly as a commensal organism in both humans and pets, and is an agent of disease. Staphylococci are potentially highly virulent pathogens which require urgent medical attention. In addition, Staphylococci remain a threat within hospital environments, where they can quickly spread across a patient population. This book explores the organisms' resistance to many compounds used to treat them, treatment failure and multidrug resistant staphylococci, amongst other related topics. Focuses not only on man and animal staphylococcal diseases, but on the role of shared household in man-to-pet (and vice versa) transmission Underlines the importance of professional exposure to mammals (i.e. veterinary and farm personnel) in the establishment of shared colonization's and related diseases Highlights the impact of shared staphylococci and virulence determinants in human and veterinary pathology Sheds light on the way staphylococci may be recognized in clinical laboratories

Download or read book DNA Electrophoresis written by Katsuhiro Hanada and published by Humana. This book was released on 2020-01-28 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the latest techniques used by researchers to study DNA electrophoresis, with focus on various species including bacteria, yeasts, and mammalian cells. The chapters in this book cover topics such as two-dimensional gel electrophoresis; DNA replication; pulsed-field gel electrophoresis; ChIP; and post-labeling/PAGE method for detection of DNA adducts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, DNA Electrophoresis: Methods and Protocols is a valuable resource for any researchers looking to learn more about this developing field.

Download or read book Mims Medical Microbiology E Book written by Richard Goering and published by Elsevier Health Sciences. This book was released on 2018-02-27 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: Learn all the microbiology and basic immunology concepts you need to know for your courses and exams. Now fully revised and updated, Mims’ clinically relevant, systems-based approach and abundant colour illustrations make this complex subject easy to understand and remember. Learn about infections in the context of major body systems and understand why these are environments in which microbes can establish themselves, flourish, and give rise to pathologic changes. This systems-based approach to microbiology employs integrated and case-based teaching that places the ‘bug parade’ into a clinical context. Effectively review for problem-based courses with the help of chapter introductions and ‘Lessons in Microbiology’ text boxes that highlight the clinical relevance of the material, offer easy access to key concepts, and provide valuable review tools. Approach microbiology by body system or by pathogen through the accompanying electronic ‘Pathogen Parade’ – a quickly searchable, cross-referenced glossary of viruses, bacteria and fungi A new electronic ‘Vaccine Parade’ offers quick-reference coverage of the most commonly used vaccines in current clinical practice Deepen your understanding of epidemiology and the important role it plays in providing evidence-based identification of key risk factors for disease and targets for preventative medicine. Grasp and retain vital concepts easily, with a user-friendly colour coded format, succinct text, key concept boxes, and dynamic illustrations. New and enhanced information reflects the growing importance of the human microbiota and latest molecular approaches Access the complete contents on the go via the accompanying interactive eBook, with a range of bonus materials to enhance learning and retention – includes self-assessment materials and clinical cases to check your understanding and aid exam preparation.

Download or read book Genotoxicity Assessment written by Alok Dhawan and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic toxicology is recognized by geneticists and researchers concerned with the genetic impact of man-made chemicals. In Genotoxicity Assessment: Methods and Protocols, expert researchers in the field provide comprehensive genetic toxicology protocols. These include in vitro and in vivo protocols on mutation assays, cytogenetic techniques, and primary DNA damage, assays in alternate to animal models, and updated ICH guidelines. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Genotoxicity Assessment: Methods and Protocols seeks to aid research students and scientists working in regulatory toxicology as well as biomedical, biochemical and pharmaceutical sciences.

Download or read book Genetics and Evolution of Infectious Diseases written by Michel Tibayrenc and published by Elsevier. This book was released on 2010-12-17 with total page 773 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetics and Evolution of Infectious Diseases is at the crossroads between two major scientific fields of the 21st century: evolutionary biology and infectious diseases. The genomic revolution has upset modern biology and has revolutionized our approach to ancient disciplines such as evolutionary studies. In particular, this revolution is profoundly changing our view on genetically driven human phenotypic diversity, and this is especially true in disease genetic susceptibility. Infectious diseases are indisputably the major challenge of medicine. When looking globally, they are the number one killer of humans and therefore the main selective pressure exerted on our species. Even in industrial countries, infectious diseases are now far less under control than 20 years ago. The first part of this book covers the main features and applications of modern technologies in the study of infectious diseases. The second part provides detailed information on a number of the key infectious diseases such as malaria, SARS, avian flu, HIV, tuberculosis, nosocomial infections and a few other pathogens that will be taken as examples to illustrate the power of modern technologies and the value of evolutionary approaches. Takes an integrated approach to infectious diseases Includes contributions from leading authorities Provides the latest developments in the field

Download or read book DNA Electrophoresis written by Svetlana Makovets and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as 2-dimentional gel electrophoresis as the major approach. These include methods and techniques such as 2-dimentional gel electrophoresis, DNA electrophoresis under conditions in which DNA molecules are completely or partially denatured during the runs, Pulse Field Gel Electrophoresis, electrophoresis coupled to fluorescence in situ hybridization, as well as protein-DNA interactions studied using electrophoreses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, DNA Electrophoresis: Methods and Protocols aids scientists in continuing to study DNA dynamics both in live cells and in test tubes.

Download or read book The Nucleic Acid Protocols Handbook written by Ralph Rapley and published by Springer Science & Business Media. This book was released on 2008-06-29 with total page 997 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."

Download or read book Pulsed Field Gel Electrophoresis written by Margit Burmeister and published by Humana Press. This book was released on 2013-09-27 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: e1ectr0ph0re515 (PF6E) wa51nvented 6are1y 10 year5 a90, 6ut ha5 a1ready 6ec0me a maj0r techn14ue m01ecu- 610109y, 1ar w1th hundred5 0f 0ften the ca5e w1th new techn14ue5 the1r 5ta9e5, m05t pu611cat10n5 are 0- dated 6y the t1me they reach pr1nt. F0r rea50n, a num6er 0fthe auth0r5 0f 600k have effect1ve1y 5erved ph0ne c0n5u1tant5 0n PF6E app11cat10n5 f0r 5evera1 year5. 8y n0w, many 0f the maj0r meth0d5 have 6een te5ted and 1mpr0ved that we fee1 a 600k 0f techn14ue5 W0ve Dur1n9 the prepara- Meth0d5, and 7~, tw0 0ther 600k5 rev1ew1n9 PF6E were pu6115hed. H0wever, the pre5ent w0rk the f1r5t t0 1nc0rp0rate the pr0t0c01 5ty1e, the ha11mark 0f the Meth0d51n that ha5 made that appr0ach the pa5t. 1n add1t10n, many 0f the chapter51nc1ude 0n he1pfu11n 5trate91e5 p1ann1n9 exper1ment5. 7he part 0f the 600k c0nta1n5 the0r1e5 and 065ervat10n5 e1uc1dat1n9 the c0mp1ex 6ehav10r 0f DNA dur1n9 PF6E. F0r any 91ven app11cat10n 0f PF6E, the reader p- ceed 6y a a55em611n9 pr0t0c01 fr0m m0re than 0ne chapter (e. 9. , CHEF, preparat10n 0f a9ar05e 610ck5, and mapp1n9 5trate91e5). 70 he1p pr0ce55, th15 we have tr1ed hard t0 pr0v1de an 1ndex and cr055-reference5 that ea5y acce55 t0 the meth0d5. 1t may 6e u5efu1 t0 read chapter5 n0t 1mmed1ate1y re1ated t0 the cat10n m1nd. F0r examp1e, a u5er 0f CHEF may f1nd he1pfu1 h1nt51n the chapter5 0n F16E, 0FA6E, 0r 7AFE. Certa1n meth0d5, 5uch a5 the preparat10n 0f yea5t marker5, are d0ne 50mewhat d1fferent1y d1fferent 1a65, and the reader f1nd 5evera1 pr0- fr0m wh1ch t0 ch005e.

Download or read book Bacteriophages written by Martha R. J. Clokie and published by Humana. This book was released on 2010-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, Volume 2: Molecular and Applied Aspects examines a multitude of topics, including the bacteriophage genomics, metagenomics, transcriptomics, and proteomics, along with applied bacteriophage biology. Written in the highly successful Methods in Molecular BiologyTM series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.

Download or read book Methicillin Resistant Staphylococcus Aureus MRSA Protocols written by Yinduo Ji and published by Humana. This book was released on 2019-09-16 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens.

Download or read book Electrophoretic Techniques written by Colin F. Simpson and published by . This book was released on 1983 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: