Download or read book Proteomic Approaches to Studying Drug Targets and Resistance in Plasmodium written by and published by . This book was released on 2004 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ever increasing drug resistance by Plasmodium falciparum, the most virulent of human malaria parasites, is creating new challenges in malaria chemotherapy. The entire genome sequences of P. falciparum and the rodent malaria parasite, P. yoelii are now available. Extensive genome sequence data from other Plasmodium species including another important human malaria parasite, P. vivax are also available. Powerful research techniques coupled to genomic resources are needed to help identify new drug and vaccine targets against malaria. Applied to Plasmodium, proteomics combines high-resolution protein or peptide separation with mass spectrometry and computer software to rapidly identify large numbers of proteins expressed from various stages of parasite development. Proteomic methods can be applied to study sub-cellular localization, cell function, organelle composition, changes in protein expression patterns in response to drug exposure, drug-protein binding and validation of data from genomic annotation and transcript expression studies. Recent high-throughput proteomic approaches have provided a wealth of protein expression data on P. falciparum, while smaller-scale studies examining specific drug-related hypotheses are also appearing. Of particular interest is the study of mechanisms of action and resistance of drugs such as the quinolines, whose targets currently may not be predictable from genomic data. Coupling the Plasmodium sequence data with bioinformatics, proteomics and RNA transcript expression profiling opens unprecedented opportunities for exploring new malaria control strategies. This review will focus on pharmacological research in malaria and other intracellular parasites using proteomic techniques, emphasizing resources and strategies available for Plasmodium.
Download or read book New Strategies for Drug Discovery and Development for Plasmodium Falciparum written by and published by . This book was released on 2000 with total page 33 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malaria continues as a major health threat throughout the tropical world and potential demand for antimalarials is higher than for any other medication yet the world faces a crisis-drug resistance is emerging and spreading faster than drugs are being developed and the flow in the pipeline of new drugs has all but stopped. This represents a particular threat to the US Military. In a short time there may be parts of the world where no effective antimalarial drug is available. The recent emergence of multidrug resistant malaria parasites has intensified this problem. Recognizing this emerging crisis, it is necessary to identify new strategies for the identification and development of new antimalarials. The goal of this work is the development of a framework for antimalarial drug development into the 21st century. A new strategy for drug development is urgently needed. Current drugs are based on a small number of target molecules or lead compounds and in most cases the target of drug action is yet to be identified. Resistance is emerging rapidly and the mechanisms of resistance are poorly understood. The identification of new targets or new candidate drugs based on an understanding of the parasite biology are key elements in this new strategy. Clearly the development of a new antimalarial will require both basic and applied research working in concert with one another. The goal of this work is to use a molecular genetic approach both in the identification of new drug targets and in the investigation of mechanisms of drug resistance. The research has focused on the two objectives, namely the analysis of critical genes in the Plasmodium falciparum for their role in drug resistance and as potential new drug targets, including the analysis of gene expression in response to drug treatment using the method of Serial Analysis of Gene Expression and the use of DNA Chip technology in the analysis of the yeast heterologous system.
Download or read book High throughput Prioritization of Putative Drug Targets in the Malaria Parasite Plasmodium Falciparum written by Misha Le Grange and published by . This book was released on 2015 with total page 202 pages. Available in PDF, EPUB and Kindle. Book excerpt: Drug resistance to almost all known antimalarials is widespread and is rapidly increasing. This resistance is due to the over and misuse of these antimalarials, thus new antimalarial drugs are necessary to help in the prevention and cure of this widespread disease. Continuous in-depth studies are being done on a handful of putative targets for future exploitation and use, but not many resources are available that focus on performing data mining and target identification on the complete malaria genome, together with relations to chemical compounds. The DISCOVERY Database is a web-based system, developed for the in silico selection of drug target proteins and lead compounds. It is a database filled with malaria information and aspects that might influence the druggability of a malaria parasite protein and guide a scientist in choosing the right ligand for a protein. DISCOVERY can aid in attempting to predict the interaction of ligands with proteins of interest, associating chemical compound with malaria proteins and selective chemical similarity searches. It can be used to mine information on malaria proteins, predict ligands and compare human and mosquito host characteristics. DISCOVERY2 was developed in Java with NetBeans. The protein sequences for the Plasmodium spp. included in DISCOVERY were downloaded from PlasmoDB; the Homo sapiens proteins were downloaded from Ensembl and the Anopheles gambiae proteins was downloaded from VectorBase. Even though DISCOVERY is primarily focused on Plasmodium falciparum it also contains information for all proteins from Plasmodium vivax, Plasmodium yoelii, Plasmodium knowlesi, Plasmodium chabaudi and Plasmodium berghei as well for the human vector and mosquito host. Protein information includes sequences and annotations, functional predictions, gene ontology terms, orthology information, structural information, metabolic pathways, predicted putative protein-ligand interactions, druggability predictions and literature links. Chemical compounds are also included. Recently approaches have illustrated the value of predicting the association of chemical compounds with putative drug targets, especially when the targets of compounds, like the Glaxo Smith Kline dataset with known activity against the parasite may be extrapolated, using protein-ligand interaction databases, like ChemProt. DISCOVERY attempts to use a similar approach in associating chemical compounds with malaria proteins, using sequence homology, and also selective chemical similarity searches. Chapter 1 of this dissertation is a literature review focusing on the in silico identification of potential drug targets. It also mentions a few techniques/approaches with which to accomplish this as well as target databases that can be used to help in the identification process. Chapter 2 describes the steps taken to run and score the Plasmodium falciparum proteins in a high throughput manner through DISCOVERY. Chapter 3 gives four case studies from DISCOVERY, a protein that had a low weighted score, a protein with a very high weighted score and two proteins with weighted scores in between the other two. And Chapter 4 concludes by looking at how researchers can use this study as a starting point. In this dissertation, DISCOVERY2 was used, in conjunction with Taverna pipelines, to study all Plasmodium falciparum proteins in a high throughput manner to be able to identify possible drug targets that might be of importance for future drug identification.
Download or read book Chemical Proteomics written by Gerard Drewes and published by Humana Press. This book was released on 2011-11-08 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The multidisciplinary science of chemical proteomics studies how small molecules of synthetic or natural origin bind to proteins and modulate their function. In Chemical Proteomics: Methods and Protocols, expert researchers in the field provide key techniques to investigate chemical proteomics focusing on analytical strategies, how probes are generated, techniques for the discovery of small molecule targets and the probing of target function, and small molecule ligand and drug discovery. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chemical Proteomics : Methods and Protocols seeks to provide methodologies that will contribute to a wider application of chemical proteomics methods in biochemical and cell biological laboratories.
Download or read book From Proteins to Proteomics written by Sanjeeva Srivastava and published by CRC Press. This book was released on 2022-12-29 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteomics aims to study all the proteins of human and other living systems, as well as their properties to provide an integrated view of cellular processes. The study of proteomics involves the application of rapidly evolving high-throughput technologies and new platforms that are coming forward regularly, providing versatile novel tools for biomedical and pharmaceutical applications. This book provides a detailed understanding of the basics of proteins and proteomics, gel based-proteomics techniques, basics of mass spectrometry and quantitative proteomics, interactomics: basics and applications, and advancements in proteomics. It also covers basic knowledge about sample preparation, mass spectrometry workflow, different chromatography technologies and quantitative proteomics. The text highlights the application and challenges of various high-throughput integrated proteomics technologies capable of fast and accurate screening of thousands of biomolecules, which are found to be very effective for studying disease pathobiology and identification of next-generation biomarkers and potential drug/vaccine targets; and are therefore considered valuable tools for multidisciplinary research. Features Basics of proteins and proteomics techniques In-depth understanding of mass spectrometry and quantitative proteomics An overview of interactomics and its application for translational research Advancement in the field of proteomics and challenges in clinical applications We hope the knowledge gained from reading this book will intrigue and motivate young minds to explore future opportunities in the constantly evolving field of proteomics.
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 2002 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Download or read book Methods in Animal Proteomics written by Philip Whitfield and published by John Wiley & Sons. This book was released on 2011-09-13 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of proteomics provides researchers with a better understanding of disease and physiological processes in animals. Methods in Animal Proteomics will provide animal scientists and veterinarians currently researching these topics in domestic animals a firm foundation in the basics of proteomics methodology, while also reviewing important advances that will be of interest to established researchers in the field. Chapters will provide practical information on a range of topics including protein identification and separation, bioinformatics, and applications to disease and reproduction research. This text will be written by leading international proteomics experts and essential for researchers in the fields of animal biology and veterinary medicine.
Download or read book Target Discovery and Validation written by Alleyn T. Plowright and published by John Wiley & Sons. This book was released on 2020-02-18 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt: The modern drug developers? guide for making informed choices among the diverse target identification methods Target Discovery and Validation: Methods and Strategies for Drug Discovery offers a hands-on review of the modern technologies for drug target identification and validation. With contributions from noted industry and academic experts, the book addresses the most recent chemical, biological, and computational methods. Additionally, the book highlights techologies that are applicable to ?difficult? targets and drugs directed at multiple targets, including chemoproteomics, activity-based protein profiling, pathway mapping, genome-wide association studies, and array-based profiling. Throughout, the authors highlight a range of diverse approaches, and target validation studies reveal how these methods can support academic and drug discovery scientists in their target discovery and validation research. This resource: -Offers a guide to identifying and validating targets, a key enabling technology without which no new drug development is possible -Presents the information needed for choosing the appropriate assay method from the ever-growing range of available options -Provides practical examples from recent drug development projects, e. g. in kinase inhibitor profiling Written for medicinal chemists, pharmaceutical professionals, biochemists, biotechnology professionals, and pharmaceutical chemists, Target Discovery and Validation explores the current methods for the identification and validation of drug targets in one comrpehensive volume. It also includes numerous practical examples.
Download or read book Malaria Research in Southeast Asia written by Viroj Wiwanitkit and published by Nova Publishers. This book was released on 2007 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on "malaria", specifically covering the overview of malarial research in Southeast Asia, the area with a high prevalence of this disease. Clinical aspects, scientific laboratory aspects, public health aspects, as well as the social sciences relating to this important tropical disease observed and studied in Southeast Asia, are the focus of this important and timely book. In addition, the diagnostic guideline and clinical practice guidelines of the above-mentioned conditions are presented.
Download or read book Probing Intracellular Regulation written by Christian Neri and published by Bentham Science Publishers. This book was released on 2013-11-04 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genome science or genomics is essential to advancing knowledge in the fields of biology and medicine. Specifically, researchers learn about the molecular biology behind genetic expression in living organisms and related methods of treating human genetic diseases (including gene therapy). Advances in Genome Science is an e-book series which provides a multi-disciplinary view of some of the latest developments in genome research, allowing readers to capture the essentiality and diversity of genomics in contemporary science. The second volume of this ebook series contains a selection of articles on intracellular gene regulation and expression in human disease such as arthritis, Alzheimer’s disease, Nijmegen Breakage Syndrome, Tumors and Malaria among other diseases. The volume also features some chapters on plant genetics
Download or read book Antiparasitic and Antibacterial Drug Discovery written by Paul M. Selzer and published by John Wiley & Sons. This book was released on 2009-03-23 with total page 509 pages. Available in PDF, EPUB and Kindle. Book excerpt: Addressing parasitic diseases and those caused by bacteria, this much needed reference and handbook provides a unique insight into the approach adopted by commercial science towards infectious diseases, including the work of medicinal chemists. Many of the authors are scientists with hands-on experience of drug discovery units within the pharmaceutical industry. In addition, the text covers efforts towards drug development in infectious diseases from academic groups and non profit organizations.
Download or read book Reflections on a Century of Malaria Biochemistry written by Irwin Sherman and published by Academic Press. This book was released on 2011-08-29 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malaria is one of the most common infectious diseases and an enormous public health problem. Each year it causes disease in approximately 650 million people and kills between 1 and 3 million, most of them young children in Sub-Saharan Africa. This book provides an overview of the research that has been done in malaria biochemistry in the quest to find a cure. It discusses how our understanding has helped us to develop better diagnostics and novel chemotherapies. Researchers will find having all of this information in one volume, annotated with personal reflections from a leader in the field, invaluable given the big push being made on various fronts to use the latest drug discovery tools to attack malaria and other developing country diseases. Reviews the past 100 years of malaria biochemistry research providing researchers with an overview of the investigations that have been undertaken in this field Chronicles both biochemical successes and failures
Download or read book Activity Based Protein Profiling written by Benjamin F. Cravatt and published by Springer. This book was released on 2019-01-25 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions.
Download or read book Malaria Control and Elimination written by Frédéric Ariey and published by Methods in Molecular Biology. This book was released on 2020-07-17 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Proteasome Ubiquitin Protein Degradation Pathway written by Peter Zwickl and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume gives an overview of pro tea some-mediated protein degradation and the regulatory role of the ubiquitin system in cellular proteolysis. The first chapter describes the molecular evolution of the proteasome and its associated activators, i. e. , the 20S core, the base and the lid of the 19S cap, and the 11 S regulator. The ensuing chapter gives an overview of the structure and assembly of the 20S proteasome and the regulation of the archaeal proteasome by PAN. The third contribution summarizes our knowledge on the eukaryotic 26S proteasome and its regulation by the 19S regu lator, followed by a chapter devoted to the llS regulator, which elucidates the structural basis for the 11 S-mediated activation of the 20S proteasome. The fifth chapter reviews in detail the role of the proteasome in the immune response. The subsequent chapter of the natural substrates of the gives a comprehensive description proteasome and their recognition by the enzymes of the ubiqui tination machinery. The penultimate chapter rounds up the in formation on intracellular distribution of proteasomes in yeast and mammalian cells, while the last contribution highlights proteasome inhibitors, tools which proved to be very valuable for dissecting the cellular roles of the proteasome and which might turn out to be of pharmacological importance.
Download or read book Resistance of Malaria Parasites to Drugs written by World Health Organization. Scientific Group on Resistance of Malaria Parasites to Drugs and published by . This book was released on 1965 with total page 76 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Modelling Microorganisms in Food written by Stanley Brul and published by Elsevier. This book was released on 2007-03-12 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Predicting the growth and behaviour of microorganisms in food has long been an aim in food microbiology research. In recent years, microbial models have evolved to become more exact and the discipline of quantitative microbial ecology has gained increasing importance for food safety management, particularly as minimal processing techniques have become more widely used. These processing methods operate closer to microbial death, survival and growth boundaries and therefore require even more precise models. Written by a team of leading experts in the field, Modelling microorganims in food assesses the latest developments and provides an outlook for the future of microbial modelling. Part one discusses general issues involved in building models of microbial growth and inactivation in foods, with chapters on the historical background of the field, experimental design, data processing and model fitting, the problem of uncertainty and variability in models and modelling lag-time. Further chapters review the use of quantitative microbiology tools in predictive microbiology and the use of predictive microbiology in risk assessment. The second part of the book focuses on new approaches in specific areas of microbial modelling, with chapters discussing the implications of microbial variability in predictive modelling and the importance of taking into account microbial interactions in foods. Predicting microbial inactivation under high pressure and the use of mechanistic models are also covered. The final chapters outline the possibility of incorporating systems biology approaches into food microbiology. Modelling microorganisms in food is a standard reference for all those in the field of food microbiology. Assesses the latest developments in microbial modelling Discusses the issues involved in building models of microbial growth Chapters review the use of quantitative microbiology tools in predictive microbiology
