Download or read book Protein based Engineered Nanostructures written by Aitziber L. Cortajarena and published by Springer. This book was released on 2016-09-27 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is devoted to the engineering of protein-based nanostructures and nanomaterials. One key challenge in nanobiotechnology is to be able to exploit the natural repertoire of protein structures and functions to build materials with defined properties at the nanoscale using “bottom-up” strategies. This book addresses in an integrated manner all the critical aspects that need to be understood and considered to design the next generation of nano-bio assemblies. The book covers first the fundamentals of the design and features of the protein building blocks and their self-assembly illustrating some of the most relevant examples of nanostructural design. Finally, the book contains a section dedicated to demonstrated applications of these novel bioinspired nanostructures in different fields from hybrid nanomaterials to regenerative medicine. This book provides a comprehensive updated review of this rapidly evolving field.
Download or read book Protein nanoparticle Co engineering written by Rubul Mout and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Direct cytoplasmic delivery of gene editing nucleases such CRISPR/Cas9 systems and therapeutic proteins provides enormous opportunities in curing human genetic diseases, and assist research in basic cell biology. One approach to attain such a goal is through engineering nanotechnological tools to mimic naturally existing intra- and extracellular protein delivery/transport systems. Nature builds transport systems for proteins and other biomolecules through evolution-derived sophisticated molecular engineering. Inspired by such natural assemblies, I employed molecular engineering approaches to fabricate self-assembled nanostructures to use as intracellular protein delivery tools. Briefly, proteins and gold nanoparticles were co-engineered to carry complementary electrostatic recognition elements. When these materials were mixed together, they formed highly sophisticated, multi-layered, and hierarchical self-assembled nanostructures of few hundred-nanometer size. These structures carried a large number of engineered proteins, got fused to cell membrane upon incubation, and delivered the encapsulated protein content directly into cell cytoplasm. Using this technology, we delivered a wide range of proteins, and CRISPR/Cas9-ribonucleoprotein that resulted high efficient gene editing.
Download or read book Co engineering Proteins and Nanoparticles for Fundamental Study and Delivery Applications written by Moumita Ray and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Engineered nanoparticles provide a powerful scaffold for interfacing with proteins. The nanoparticle surface can be tailored to present recognition elements, providing surface complementarity to interact with protein surfaces. In this thesis, I have explored both the fundamental and the applied aspects of this interaction. On the fundamental side, I have co-engineered the nanoparticles and the proteins to generate robust dyads with strong binding affinity even at high salt concentration. Fluorescence titrations and docking studies were carried out to quantify the binding properties of the nanoparticles and proteins. Those studies revealed the prospect of tuning the affinity between the nanoparticles and proteins by co-engineering. On the application side, I have employed nanoparticle-protein interaction to fabricate self-assembled nanostructures to be used as intracellular protein delivery tools. In the first segment, nanoparticles and proteins were assembled to form nanoparticle stabilized capsules (NPSCs) for nuclear trafficking of proteins. The first non-peptide synthetic nuclear localization signal based on boronate was discovered, as well, using NPSC delivery platform. In the second segment, proteins and nanoparticles were co-engineered to self-assemble into hierarchical multi-layered nanostructures. These nanostructures were employed to deliver encapsulated proteins into cell cytosol, establishing a general strategy for protein delivery. Using this technology, I have delivered CRISPR/Cas9-ribonucleoprotein that resulted in highly efficient gene editing. Further, I have created an integrated nanotechnology/biology approach to engineer macrophages in vitro, thus, greatly enhancing their ability to phagocytose tumor cells, providing a new immunotherapeutic strategy for cancer therapy.
Download or read book Nanostructures for the Engineering of Cells Tissues and Organs written by Alexandru Mihai Grumezescu and published by William Andrew. This book was released on 2018-02-14 with total page 631 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanostructures for the Engineering of Cells: Tissues and Organs showcases recent advances in pharmaceutical nanotechnology, with particular emphasis on tissue engineering, organ and cell applications. The book provides an up-to-date overview of organ targeting and cell targeting using nanotechnology. In addition, tissue engineering applications, such as skin regeneration are also discussed. Written by a diverse range of international academics, this book is a valuable research resource for researchers working in the biomaterials, medical and pharmaceutical industries. - Explains how nanomaterials regulate different cell behavior and function as a carrier for different biomolecules - Shows how nanobiomaterials and nanobiodevices are used in a range of treatment areas, such as skin tissue, wound healing and bone regeneration - Discusses nanomaterial preparation strategies for pharmaceutical application and regenerative medicine
Download or read book Protein Self Assembly written by Jennifer J. McManus and published by Humana. This book was released on 2020-08-08 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.
Download or read book Carbohydrate Nanotechnology written by Keith J. Stine and published by John Wiley & Sons. This book was released on 2015-11-02 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introducing the emerging field carbohydrate nanostructures, this book will be a unique resource for interested researchers to learn a range of methods of applying the field to their own work. Greater access, as well as greater collaboration, to this new interdisciplinary field is intended for both synthetic carbohydrate chemists and researchers in nanoscience related fields. It covers: the main types of nanostructures presently under investigation for modification by carbohydrates, including nanoparticles, nanorods, magnetic particles, dendrimers, nanoporous, and surface confined structures overview and introduction to the field of carbohydrate nanotechnology, and especially its applications to its biological systems Provides a unique resource for researchers to learn about the techniques used to characterize the physical and biological properties of carbohydrate-modified nanostructures
Download or read book Engineered Nanostructures for Therapeutics and Biomedical Applications written by Ajeet Kumar Kaushik and published by Woodhead Publishing. This book was released on 2022-08-31 with total page 345 pages. Available in PDF, EPUB and Kindle. Book excerpt: Engineered Nanostructures for Therapeutics and Biomedical Applications offers a single reference for a diverse biomedical readership to learn about the application of nanotechnology in biomedicine and biomedical engineering, from past developments to current research and future prospects. This book sets out a broad selection of biomedical and therapeutic applications for nanostructures, including bioimaging, nanorobotics, orthopedics, and tissue engineering, offering a useful, multidisciplinary approach. Each chapter discusses challenges faced in each discipline, including limiting factors, biocompatibility, and toxicity, thus enabling the reader to make informed decisions in their research.This book is a comprehensive, broad overview of the role and significance of nanomaterials and their composites that also includes discussions of key aspects in the field of biomedicine. It will be of significant interest to academics and researchers in materials science and engineering, biomedicine and biomedical engineering, chemical engineering, pharmaceutics, bioimaging, and nanorobotics. - Provides a broad overview of the many applications of nanomaterials and nanotechnology in biomedicine and engineering - Offers a multidisciplinary approach that will appeal to a diverse readership, including those in biomedical engineering, materials science, biomedicine, and pharmaceutics - Includes challenges faced and limiting factors for each application, allowing readers to make an informed decision when using nanomaterials in their research
Download or read book DNA Engineered Noble Metal Nanoparticles written by Ignác Capek and published by John Wiley & Sons. This book was released on 2015-04-13 with total page 672 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is a growing interest in the use of nanoparticles modified with DNAs, viruses, peptides and proteins for the rational design of nanostructured functional materials and their use in biosensor applications. The challenge is to control the organization of biomolecules on nanoparticles while retaining their biological activity as potential chemical and gene therapeutics. These noble metal nanoparticles/biomolecules conjugates have specific properties and therefore they are attractive materials for nanotechnology in biochemistry and medicine. In this book, the author review work performed dealing with the DNA structure and functionalities, interactions between DNA, noble metal nanoparticles, surface active agents, solvents and other additives. Particular attention is given to how the DNA's chain length and the DNA conformation affect the interaction and structure of the nanoconjugates and nanostructures that are formed. Also discussed are the recent advances in the preparation, characterization, and applications of noble metal nanoparticles that are conjugated with DNA aptamers and oligomers. The advantages and disadvantages of functionalized nanoparticles through various detection modes are highlighted, including colorimetry, fluorescence, electrochemistry, SPR, and, mass spectrometry for the detection of small molecules and biomolecules. The functionalized noble metal nanoparticles are selective and sensitive for the analytes, showing their great potential in biosensing. Furthermore, this book reviews recent progress in the area of DNA-noble metal nanoparticles based artificial nanostructures, that is, the preparation, collective properties, and applications of various DNA-based nanostructures are also described.
Download or read book Virus Based Nanomaterials and Nanostructures written by Dong-Wook Han and published by MDPI. This book was released on 2020-06-23 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt: A virus is considered a nanoscale organic material that can infect and replicate only inside the living cells of other organisms, ranging from animals and plants to microorganisms, including bacteria and archaea. The structure of viruses consists of two main parts: the genetic material from either DNA or RNA that carries genetic information, and a protein coat, called the capsid, which surrounds and protects the genetic material. By inserting the gene encoding functional proteins into the viral genome, the functional proteins can be genetically displayed on the protein coat to form bioengineered viruses. Therefore, viruses can be considered biological nanoparticles with genetically tunable surface chemistry and can serve as models for developing virus-like nanoparticles and even nanostructures. Via this process of viral display, bioengineered viruses can be mass-produced with lower cost and potentially used for energy and biomedical applications. This book highlights the recent developments and future directions of virus-based nanomaterials and nanostructures. The virus-based biomimetic materials formulated using innovative ideas were characterized for the applications of biosensors and nanocarriers. The research contributions and trends on virus-based materials covering energy harvesting devices to tissue regeneration in the last two decades are discussed.
Download or read book Textbook of Nanoscience and Nanotechnology written by B.S. Murty and published by Springer Science & Business Media. This book was released on 2013-12-06 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is meant to serve as a textbook for beginners in the field of nanoscience and nanotechnology. It can also be used as additional reading in this multifaceted area. It covers the entire spectrum of nanoscience and technology: introduction, terminology, historical perspectives of this domain of science, unique and widely differing properties, advances in the various synthesis, consolidation and characterization techniques, applications of nanoscience and technology and emerging materials and technologies.
Download or read book Protein Nanoparticle Interactions written by Masoud Rahman and published by Springer Science & Business Media. This book was released on 2013-06-24 with total page 95 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, the fabrication of nanomaterials and exploration of their properties have attracted the attention of various scientific disciplines such as biology, physics, chemistry, and engineering. Although nanoparticulate systems are of significant interest in various scientific and technological areas, there is little known about the safety of these nanoscale objects. It has now been established that the surfaces of nanoparticles are immediately covered by biomolecules (e.g. proteins, ions, and enzymes) upon their entrance into a biological medium. This interaction with the biological medium modulates the surface of the nanoparticles, conferring a “biological identity” to their surfaces (referred to as a “corona”), which determines the subsequent cellular/tissue responses. The new interface between the nanoparticles and the biological medium/proteins, called “bio-nano interface,” has been very rarely studied in detail to date, though the interest in this topic is rapidly growing. In this book, the importance of the physiochemical characteristics of nanoparticles for the properties of the protein corona is discussed in detail, followed by comprehensive descriptions of the methods for assessing the protein-nanoparticle interactions. The advantages and limitations of available corona evaluation methods (e.g. spectroscopy methods, mass spectrometry, nuclear magnetic resonance, electron microscopy, X-ray crystallography, and differential centrifugal sedimentation) are examined in detail, followed by a discussion of the possibilities for enhancing the current methods and a call for new techniques. Moreover, the advantages and disadvantages of protein-nanoparticle interaction phenomena are explored and discussed, with a focus on the biological impacts.
Download or read book Healthy Past 100 written by Steven M. Teagarden DC and published by BookLocker.com, Inc.. This book was released on 2023-08-10 with total page 990 pages. Available in PDF, EPUB and Kindle. Book excerpt: Healthy Past 100 was written explicitly for those longing to be extraordinarily healthy—even past their 100th birthday. This breakthrough book is based on thousands of cutting-edge science articles identifying the causes of optimal health vs sickness and disease. Healthy Past 100 puts a wealth of cutting-edge, science-based healthcare information at your fingertips, empowering you to make the healthiest choices for you and your family. Healthy Past 100 is several books in one, and it also contains a mouthwatering keto cookbook that's exclusively based on the most nourishing ingredients you can consume. This life-changing book is based on the Short List, i.e., a distillation of the core factors involved in being healthy past 100. The Short List includes: The Metabolic Masterplan Diet: the Metabolic Masterplan Diet may be the world's most advanced ketogenic diet. Nothing improves your health as much as following this research-based, anti-inflammatory, gut-healing ketogenic diet. Nutrition and Supplements: providing your cells with all the nutrients they require to function optimally is key to a long, healthy life. Healthy Past 100 shows you exactly which nutrients you need and which ones to supplement with. Metabolism: carbohydrates and sugar damage your metabolism (the process of creating energy in your cells), causing serious health issues. Healthy Past 100 teaches you to heal and optimize your metabolism, and which tests to rely on to know—not guess—that your metabolism is as healthy as can be. Chronic Inflammation: this widespread health issue underlies all diseases, and must be healed to experience optimal health. Healthy Past 100 rids you of chronic inflammation—now and forever—paving the way to lasting health. Gut Health: most humans deal with significant gut issues. Fortunately, Healthy Past 100's innovative gut healing method leads to lifelong optimized gut health. Psychology: psychology is how you think, feel, act, behave and respond to life's circumstances. Healthy Past 100 provides the tools to heal and optimize your psychology, which is foundational to a healthy, fulfilling life. Thyroid Physiology: inefficient thyroid physiology impairs your body and mind, zaps your energy and causes many diseases. Healthy Past 100 explains how thyroid physiology works, how to heal it, and the tests determining if it's in tip-top shape. Exercise: crucial to your health and well-being, exercise must be performed regularly. Healthy Past 100 teaches you which exercises help you become healthy past 100. Detoxification: humans are routinely exposed to dangerous heavy metal toxins. Healthy Past 100 shows you how to rid these harmful metals from your body and minimize your exposure to them in the future. Oxidative Stress: oxidative stress is a primary cause of aging, sickness, disease and death. It has many causes, including carbohydrates, sugar, poor nutrition and impaired gut health. Healthy Past 100 helps you optimize oxidative stress where it matters most: within the mitochondria in your cells. Spiritual/Religious Life: extraordinary health stems from optimal physical, psychological and spiritual well-being. It's just as important to focus on your spiritual fitness as it is to heal your physical body, and Healthy Past 100 helps you do both. This pioneering book has been written to help you experience extraordinary health past your 100th birthday. If you're ready to be healthy past 100, this is the book you've been waiting for!
Download or read book Intercellular Communication in Cancer written by Mustapha Kandouz and published by Springer. This book was released on 2015-11-20 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells are by nature compelled to live in groups. They develop dependence over signaling cues received from their microenvironment, in particular from other cells, whether of their own “kind” or of a different type. Therefore, communicating with these cells is a critical aspect of their behavior and fate, as they live and die normally or as they undergo disease-related pathological changes, with dramatic repercussions. In this book, we have asked expert researchers in the field of Intercellular Communication in Cancer to provide chapters on different aspects of interaction between neighboring cells, in the context of cancer diseases. We have specifically focused our efforts on membrane-to-membrane contact-based rather than growth factors-mediated modes of intercellular communications. The contributing authors provide an extensive overview of their respective area of specialization, with an in-depth discussion of the molecular mechanisms of cell-cell interactions, the impact on tumor progression and response to therapies, as well as the cancer diagnostic value of this scientific information. This book aims to introduce essential aspects of the normal and pathological cellular fate and homeostasis to both scientists and clinicians, and also to provide established researchers with an update on the novelties and future directions this expanding field is witnessing.
Download or read book Proteins in Solution and at Interfaces written by Juan M. Ruso and published by John Wiley & Sons. This book was released on 2013-01-31 with total page 823 pages. Available in PDF, EPUB and Kindle. Book excerpt: Explores new applications emerging from our latest understanding of proteins in solution and at interfaces Proteins in solution and at interfaces increasingly serve as the starting point for exciting new applications, from biomimetic materials to nanoparticle patterning. This book surveys the state of the science in the field, offering investigators a current understanding of the characteristics of proteins in solution and at interfaces as well as the techniques used to study these characteristics. Moreover, the authors explore many of the new and emerging applications that have resulted from the most recent studies. Topics include protein and protein aggregate structure; computational and experimental techniques to study protein structure, aggregation, and adsorption; proteins in non-standard conditions; and applications in biotechnology. Proteins in Solution and at Interfaces is divided into two parts: Part One introduces concepts as well as theoretical and experimental techniques that are used to study protein systems, including X-ray crystallography, nuclear magnetic resonance, small angle scattering, and spectroscopic methods Part Two examines current and emerging applications, including nanomaterials, natural fibrous proteins, and biomolecular thermodynamics The book's twenty-three chapters have been contributed by leading experts in the field. These contributions are based on a thorough review of the latest peer-reviewed findings as well as the authors' own research experience. Chapters begin with a discussion of core concepts and then gradually build in complexity, concluding with a forecast of future developments. Readers will not only gain a current understanding of proteins in solution and at interfaces, but also will discover how theoretical and technical developments in the field can be translated into new applications in material design, genetic engineering, personalized medicine, drug delivery, biosensors, and biotechnology.
Download or read book Engineering the Filamentous Molecular Chaperone gamma Prefoldin for Construction of Protein Nanostructures written by Timothy Andrew Whitehead and published by . This book was released on 2008 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bioactivity of Engineered Nanoparticles written by Bing Yan and published by Springer. This book was released on 2017-08-17 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book brings together reviews from international experts who are exploring the biological activities of nanomaterials for medical applications or to better understand nanotoxicity. Topics include but are not limited to the following: 1) mechanistic understanding of nanostructure-bioactivity relationships; 2) the regulation of nanoparticles’ bioactivity by means of chemical modification; 3) the new methodologies and standard methods used to assess nanoparticles’ bioactivity; 4) the mechanisms involved in nanoparticle-biomolecule interactions and nanoparticle-cell interactions; and 5) biomedical applications of nanotechnology. The book will be a valuable resource for a broad readership in various subfields of chemical science, engineering, biology, environment, and medicine.
Download or read book Engineered Nanoparticles and the Environment written by Baoshan Xing and published by John Wiley & Sons. This book was released on 2016-08-29 with total page 510 pages. Available in PDF, EPUB and Kindle. Book excerpt: Details the source, release, exposure, adsorption, aggregation, bioavailability, transport, transformation, and modeling of engineered nanoparticles found in many common products and applications Covers synthesis, environmental application, detection, and characterization of engineered nanoparticles Details the toxicity and risk assessment of engineered nanoparticles Includes topics on the transport, transformation, and modeling of engineered nanoparticles Presents the latest developments and knowledge of engineered nanoparticles Written by world leading experts from prestigious universities and companies
