Download or read book Molecular Biomethods Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2008-11-04 with total page 1105 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.
Download or read book Protein and Cell Biomethods Handbook written by and published by . This book was released on 2008-01-01 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Medical BioMethods Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2007-10-09 with total page 635 pages. Available in PDF, EPUB and Kindle. Book excerpt: John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.
Download or read book The Protein Protocols Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 1996 with total page 759 pages. Available in PDF, EPUB and Kindle. Book excerpt: -- 145 concise, step-by-step protocols for the analysis of proteins and peptides -- Provides more than three times as many protocols than the competitors' manuals -- in the incomparable Methods in Molecular Biology "TM" style -- at an affordable price -- Combound in a large 7 x l0 inch format for easy benchtop use -- An indispensable reference for industry-based researchers, professors, and their students
Download or read book A Laboratory Guide for Cellular and Molecular Plant Biology written by I. Negrutiu and published by Birkhäuser. This book was released on 2013-03-09 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide comes at a time when several other method books have already been published in this field. Is this one different from the others? Yes and no. There was no attempt made to be comprehensive. Rather, data were brought to bear on areas where enough competence has been gathered in our laboratories and to complement recent method books (many of which cover extensively various aspects of molecular biology) in those matters which appeared to us somewhat neglected. There was a constant preoccupation and effort to provide miniaturized proce dures that are both simple and time-saving. Interest was devoted to standardized procedures and culture conditions, avoiding dogmas such as those giving excessive importance to sophisticated culture media with endless adjustments for local or personal considerations. The key to success is the quality of the plant material serving as a source of cells. Consequently, isolation. extraction or culture techniques can be simplified and standardized. This is symptomatic for our times as it marks the end of a period when methodological matters were frequently above the biological problems. The times of "methods above all" is basically over, despite the fact that many of us still believe that, say, tissue culture is a "science" per se. By presenting a few original techniques we believe that one seriously reduces the empiricism still prevailing in this area of research.
Download or read book Somatic Embryogenesis Fundamental Aspects and Applications written by Víctor M Loyola-Vargas and published by Springer. This book was released on 2016-06-06 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses basic and applied aspects of somatic embryogenesis, one of the most powerful tools in plant biotechnology. It is divided into three parts; Part I includes topics such as the history of this research field, how differentiated plant cells can (re)acquire totipotency, molecular features, as well as the epigenetics and proteomics of somatic embryogenesis. Part II covers the somatic embryogenesis of different crops, such as Agave spp. maize, Cocos nucifera, Bixa orellana, Capsicum spp., Coffea spp., Musa spp., Pinus spp., and Arabidopsis thaliana. Various applications, like scale-up propagation and genetic engineering are discussed in detail in Part III. The book will appeal to plant scientists, plant breeders and experts working in industry.
Download or read book Recent Advances of Epigenetics in Crop Biotechnology written by Clelia De-la-Peña and published by Frontiers Media SA. This book was released on 2016-05-31 with total page 191 pages. Available in PDF, EPUB and Kindle. Book excerpt: Epigenetics is a new field that explains gene expression at the chromatin structure and organization level. Three principal epigenetic mechanisms are known and hundreds of combinations among them can develop different phenotypic characteristics. DNA methylation, histone modifications and small RNAs have been identified, and their functions are being studied in order to understand the mechanisms of interaction and regulation among the different biological processes in plants. Although, fundamental epigenetic mechanisms in crop plants are beginning to be elucidated, the comprehension of the different epigenetic mechanisms, by which plant gene regulation and phenotype are modified, is a major topic to develop in the near future in order to increase crop productivity. Thus, the importance of epigenetics in improving crop productivity is undoubtedly growing. Current research on epigenetics suggest that DNA methylation, histone modifications and small RNAs are involved in almost every aspect of plant life including agronomically important traits such as flowering time, fruit development, responses to environmental factors, defense response and plant growth. The aim of this Research Topic is to explore the recent advances concerning the role of epigenetics in crop biotechnology, as well as to enhance and promote interactions among high quality researchers from different disciplines such as genetics, cell biology, pathology, microbiology, and evolutionary biology in order to join forces and decipher the epigenetic mechanisms in crop productivity.
Download or read book Wilson and Walker s Principles and Techniques of Biochemistry and Molecular Biology written by Andreas Hofmann and published by Cambridge University Press. This book was released on 2018-04-19 with total page 962 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bringing this best-selling textbook right up to date, the new edition uniquely integrates the theories and methods that drive the fields of biology, biotechnology and medicine, comprehensively covering both the techniques students will encounter in lab classes and those that underpin current key advances and discoveries. The contents have been updated to include both traditional and cutting-edge techniques most commonly used in current life science research. Emphasis is placed on understanding the theory behind the techniques, as well as analysis of the resulting data. New chapters cover proteomics, genomics, metabolomics, bioinformatics, as well as data analysis and visualisation. Using accessible language to describe concepts and methods, and with a wealth of new in-text worked examples to challenge students' understanding, this textbook provides an essential guide to the key techniques used in current bioscience research.
Download or read book The Protein Protocols Handbook written by John M. Walker and published by Humana. This book was released on 2009-10-07 with total page 2032 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third edition of The Protein Protocols Handbook introduces 57 critically important new chapters, and significantly updates the previous edition's tried-and-trusted methods. The book offers over 200 key, readily reproducible protocols that ensure results.
Download or read book Epigenetics in Plants of Agronomic Importance Fundamentals and Applications written by Raúl Alvarez-Venegas and published by Springer. This book was released on 2014-07-22 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past decades, chromatin remodelling has emerged as an important regulator of gene expression and plant defense. This book provides a detailed understanding of the epigenetic mechanisms involved in plants of agronomic importance. The information presented here is significant because it is expected to provide the knowledge needed to develop in the future treatments to manipulate and selectively activate/inhibit proteins and metabolic pathways to counter pathogens, to treat important diseases and to increase crop productivity. New approaches of this kind and the development of new technologies will certainly increase our knowledge of currently known post-translational modifications and facilitate the understanding of their roles in, for example, host-pathogen interactions and crop productivity. Furthermore, we provide important insight on how the plant epigenome changes in response to developmental or environmental stimuli, how chromatin modifications are established and maintained, to which degree they are used throughout the genome, and how chromatin modifications influence each another.
Download or read book Biological Drug Products written by Wei Wang and published by John Wiley & Sons. This book was released on 2013-08-29 with total page 531 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tested and proven solutions to the challenges of biological drug product development Biological drug products play a central role in combating human diseases; however, developing new successful biological drugs presents many challenges, including labor intensive production processes, tighter regulatory controls, and increased market competition. This book reviews the current state of the science, offering readers a single resource that sets forth the fundamentals as well as tested and proven development strategies for biological drugs. Moreover, the book prepares readers for the challenges that typically arise during drug development, offering straightforward solutions to improve their ability to pass through all the regulatory hurdles and deliver new drug products to the market. Biological Drug Products begins with general considerations for the development of any biological drug product and then explores the strategies and challenges involved in the development of specific types of biologics. Divided into five parts, the book examines: Part 1: General Aspects Part 2: Proteins and Peptides Part 3: Vaccines Part 4: Novel Biologics Part 5: Product Administration/Delivery Each chapter has been prepared by one or more leading experts in biological drug development. Contributions are based on a comprehensive review and analysis of the current literature as well as the authors' first-hand experience developing and testing new drugs. References at the end of each chapter serve as a gateway to original research papers and reviews in the field. By incorporating lessons learned and future directions for research, Biological Drug Products enables pharmaceutical scientists and students to improve their success rate in developing new biologics to treat a broad range of human diseases.
Download or read book Image Analysis written by Donat P. Hader and published by CRC Press. This book was released on 2000-08-23 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Automatic image analysis has become an important tool in many fields of biology, medicine, and other sciences. Since the first edition of Image Analysis: Methods and Applications, the development of both software and hardware technology has undergone quantum leaps. For example, specific mathematical filters have been developed for quality enhancement of original images and for extraction of specific features of interest. Also, more complex programs have been developed for the analysis of object forms in distinguishing cancer cells from normal tissue cells. Just as significant, three-dimensional analysis of proteins, organelles, or macroscopic objects is even more complex. In addition, recent space-based experiments have optimized techniques for the extraction of movement parameters of numerous motile objects. The second edition of Image Analysis: Methods and Applications addresses all these new developments. Moreover, two new chapters have been added. One focuses on images on the internet, and the other discusses microscope image restoration. These chapters add significantly to the existing body of information on Internet communication protocol and environment as well as to that on image file formats considerations. The materials also include a list of internet Web sites that pertain to digital images and software along with those that relate to image processing. With these considerations in mind, Image Analysis: Methods and Application, Second Edition is of incalculable value to professionals, academics, and users of all aspects of image analysis in biology and other areas of science.
Download or read book The Biomedical Engineering Handbook written by Joseph D. Bronzino and published by CRC Press. This book was released on 2018-10-03 with total page 5430 pages. Available in PDF, EPUB and Kindle. Book excerpt: The definitive "bible" for the field of biomedical engineering, this collection of volumes is a major reference for all practicing biomedical engineers and students. Now in its fourth edition, this work presents a substantial revision, with all sections updated to offer the latest research findings. New sections address drugs and devices, personali
Download or read book The Evolution from Protein Chemistry to Proteomics written by Roger L. Lundblad and published by CRC Press. This book was released on 2005-10-14 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: Largely driven by major improvements in the analytical capability of mass spectrometry, proteomics is being applied to broader areas of experimental biology, ranging from oncology research to plant biology to environmental health. However, while it has already eclipsed solution protein chemistry as a discipline, it is still essentially an extension
Download or read book Molecular Biology written by Nancy Craig and published by Oxford University Press. This book was released on 2014-05 with total page 945 pages. Available in PDF, EPUB and Kindle. Book excerpt: The biological world operates on a multitude of scales - from molecules to tissues to organisms to ecosystems. Throughout these myriad levels runs a common thread: the communication and onward passage of information, from cell to cell, from organism to organism and ultimately, from generation to generation. But how does this information come alive to govern the processes that constitute life? The answer lies in the molecular components that cooperate through a series of carefully-regulated processes to bring the information in our genome to life. These components and processes lie at the heart of one of the most fascinating subjects to engage the minds of scientists today: molecular biology. Molecular Biology: Principles of Genome Function, Second Edition, offers a fresh approach to the teaching of molecular biology by focusing on the commonalities that exist between the three kingdoms of life, and discussing the differences between the three kingdoms to offer instructive insights into molecular processes and components. This gives students an accurate depiction of our current understanding of the conserved nature of molecular biology, and the differences that underpin biological diversity. Additionally, an integrated approach demonstrates how certain molecular phenomena have diverse impacts on genome function by presenting them as themes that recur throughout the book, rather than as artificially separated topics As an experimental science, molecular biology requires an appreciation for the approaches taken to yield the information from which concepts and principles are deduced. Experimental Approach panels throughout the text describe research that has been particularly valuable in elucidating difference aspects of molecular biology. Each panel is carefully cross-referenced to the discussion of key molecular biology tools and techniques, which are presented in a dedicated chapter at the end of the book. Molecular Biology further enriches the learning experience with full-color artwork, end-of-chapter questions and summaries, suggested further readings grouped by topic, and an extensive glossary of key terms. Features: A focus on the underlying principles of molecular biology equips students with a robust conceptual framework on which to build their knowledge An emphasis on their commonalities reflects the processes and components that exist between bacteria, archae, and eukaryotes Experimental Approach panels demonstrate the importance of experimental evidence by describing research that has been particularly valuable in the field
Download or read book Molecular Biology and Biotechnology written by John M Walker and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 589 pages. Available in PDF, EPUB and Kindle. Book excerpt: As a textbook, Molecular Biology and Biotechnology has always been immensely popular. Now in its fourth edition, it has been completely revised and updated to provide a comprehensive overview and to reflect all the latest developments in this rapidly expanding area. Written by recognised experts, the book aims to identify the impact that molecular biology has had on the development of biotechnology, with each of the nineteen chapters describing a specific subject area relevant to the subject. The impressive breadth of coverage includes areas such as plant biotechnology; food technology; vaccine development; the production of transgenic plants and animals; and the addition of an appropriate and timely new chapter devoted to bioinformatics. Presenting information in an easily assimilated form, Molecular Biology and Biotechnology makes an ideal undergraduate text. It will be of particular interest to students of biology and chemistry, as well as to scientists from outside the field requiring a rapid introduction to the subject.
Download or read book Using The Biological Literature written by Diane Schmidt and published by CRC Press. This book was released on 2001-12-06 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Provides an in-depth review of current print and electronic tools for research in numerous disciplines of biology, including dictionaries and encyclopedias, method guides, handbooks, on-line directories, and periodicals. Directs readers to an associated Web page that maintains the URLs and annotations of all major Inernet resources discussed in th
