Download or read book Practical Methods in Electron Microscopy pt 1 Glauert A M Fixation dehydration and embedding of biological specimens written by Audry M. Glauert and published by . This book was released on 1972 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Practical Methods in Electron Microscopy pt 1 Fixation dehydration and embedding of biological specimens written by Audrey M. Glauert and published by . This book was released on 1972 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Practical Methods in Electron Microscopy pt 1 Staining methods for sectioned material written by Audrey M. Glauert and published by . This book was released on 1977 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Practical Methods in Electron Microscopy pt 1 Lewis P R Staining methods for sectioned material written by Audrey M. Glauert and published by . This book was released on 1977 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Practical Methods in Electron Microscopy pt 1 Lewis P R Staining methods for sectioned material written by Audry M. Glauert and published by . This book was released on 1972 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Practical Methods in Electron Microscopy pt 1 Williams M A Autoradiography and immunocytochemistry written by Audry M. Glauert and published by . This book was released on 1977 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electron Microscopy Methods and Protocols written by M. A. Nasser Hajibagheri and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).
Download or read book Fixation Dehydration and Embedding of Biological Specimens written by Audrey M. Glauert and published by . This book was released on 1975 with total page 353 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods of Preparation for Electron Microscopy written by David G. Robinson and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes sions had an unexpected instrument at their disposal which exceeded the reso lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob lems inherent in specimen preparation for the investigation of cellular struc tures. The microtechniques applied in light microscopy were no longer appli cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus.
Download or read book Biological Specimen Preparation for Transmission Electron Microscopy written by Audrey M. Glauert and published by Princeton University Press. This book was released on 2014-07-14 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements. All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs. Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor Originally published in 1999. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.
Download or read book Electron Microscopy of Model Systems written by and published by Academic Press. This book was released on 2010-09-24 with total page 745 pages. Available in PDF, EPUB and Kindle. Book excerpt: The volume covers the preparation and analysis of model systems for biological electron microscopy. The volume has chapters about prokaryotic as well as eukaryotic systems that are used as so-called model organisms in modern cell biology. These systems include the most popular systems, such as budding and fission yeast, the roundworm C. elegans, the fly Drosophila, zebrafish, mouse, and Arabidopsis, but also organisms that are less frequently used in cell biology, such as Chlamydomonas, Dictyostelium, Trypanosoma, faltworms, Axolotl and others. In addition, tissues and tissue culture systems are also covered. These systems are used for very diverse areas of cell biology, such as cell division, abscission, intracellular transport, cytoskeletal organization, tissue regeneration and others. Moreover, this issue presents the currently most important methods for the preparation of biological specimens. This volume, however, is not a classic EM methods book. The methods are not the main focus of this issue. The main goal here is to cover the methods in the context of the specific requirements of specimen preparation for each model organism or systems. This will be the first compendium covering the various aspects of sample preparation of very diverse biological systems. - Covers the preparation and analysis of model systems for biological electron microscopy - Includes the most popular systems but also organisms that are less frequently used in cell biology - Presents the currently most important methods for the preparation of biological specimens - First compendium covering the various aspects of sample preparation of very diverse biological systems
Download or read book Essential Histology written by David H. Cormack and published by Lippincott Williams & Wilkins. This book was released on 2001 with total page 546 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Second Edition presents a compact and concise alternative to the larger histology texts on the market today. Great for students with a limited amount of time to devote to the subject. Improvements to the art program--adding more color and new illustrations--have been made to this edition.
Download or read book Methods for General and Molecular Microbiology written by C. A. Reddy and published by American Society for Microbiology Press. This book was released on 2007-08-17 with total page 2949 pages. Available in PDF, EPUB and Kindle. Book excerpt: A first source for traditional methods of microbiology as well as commonly used modern molecular microbiological methods. • Provides a comprehensive compendium of methods used in general and molecular microbiology. • Contains many new and expanded chapters, including a section on the newly important field of community and genomic analysis. • Provides step-by-step coverage of procedures, with an extensive list of references to guide the user to the original literature for more complete descriptions. • Presents methods for bacteria, archaea, and for the first time a section on mycology. • Numerous schematics and illustrations (both color and black and white) help the reader to easily understand the topics presented.
Download or read book Fundamentals of Histology written by G. P. Verma and published by New Age International. This book was released on 2001 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent Advances In Biology And Physiology Have Enormously Increased The Relevance Of Histology And Made Its Study Vitally Important. This Book Presents A Systematic Treatment Of The Histological Aspects Of Different Tissues Of Almost All The Systems Of An Organism. The Text Is Presented In A Clear And Lucid Manner, Supported By Numerous Detailed And Self-Explanatory Illustrations. The Book Is Designed As A Comprehensive Text For Both Undergraduate And Postgraduate Students Of Zoology And Physiology. Students Pursuing Medical Courses Would Also Find It Very Useful.
Download or read book Practical Methods in Electron Microscopy pt 1 Fixation dehydration and embedding of biological specimens written by Audrey M. Glauert and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Resin Microscopy and On Section Immunocytochemistry written by Geoffrey R. Newman and published by Springer Science & Business Media. This book was released on 2001-04-10 with total page 564 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since antibodies tagged with markers have been developed, immunocytochemistry has become an important method for identifying tissue substances and the localisation of nucleic acid in tissue by in situ hybridisation in molecular biology. This laboratory book covers the embedding of tissue using less sensitive epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities are discussed and results are presented so that an understanding of the techniques can be acquired.
Download or read book Atlas of Invertebrate Viruses written by Jean R. Adams and published by CRC Press. This book was released on 2017-09-18 with total page 1000 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Purpose of this book is to provide a helpful reference for invertebrate pathologist, virologists, and electron microscopists on invertebrate viruses. Investigators from around the world have shared their expertise in order introduce scientists to the exciting advances in invertebrate virology.
