Download or read book Phylogenetic Characterization of Subsurface Bacteria by Analysis of 16S Ribosomal RNA and Gyrase B Gene Sequences and Phenotypic Profiles written by Dianne M. Fair and published by . This book was released on 1997 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Phylogenetic Analysis of Bacterial 16S RRNA Gene Sequences Found in Bulk Water Samples Collected Throughout a Metropolitan Area Drinking Water Distribution System written by Ben Humrighouse and published by . This book was released on 2010 with total page 77 pages. Available in PDF, EPUB and Kindle. Book excerpt: Assays for the detection of total coliforms and E. coli are used in the United States as a tool for the detection of fecal contamination in finished drinking water, as a measure to prevent illness. However, based on statistics published by a number of researchers, as many as 764 documented waterborne outbreaks occurred between 1971 and 2002, which resulted in over half a million cases of illness and 79 deaths (Reynolds, Mena, & Gerba, 2008). Also, it has been theorized that as many as 19 million cases/year of waterborne illness are caused by public water systems fed by ground water and surface water in the US. Therefore, it has been argued that coliforms are not good indicator organisms for predicting risk of waterborne illness. In order to gather more microbiological information regarding drinking water, much research has been conducted on microorganisms in planktonic phase, as well as in biofilms present in drinking water distribution systems (DWDS). Different species of bacteria may colonize surfaces (internal or external) and begin to proliferate and form biofilm (Zhang, Choi, Dionysiou, Sorial, & Oerther, 2006). Due to the fact that biofilms are consistently being established, maturing, and sloughing off, it seems reasonable that the bacteria in planktonic phase, in a distribution system, would be ideal for microbial community analysis. With this in mind, we decided to analyze bacterial genomic DNA in order to gain knowledge of the current community structures present in drinking water samples, to serve as a base for measuring future differences or shifts in community structure. Although biofilms and planktonic populations may differ to some degree i.e.: culturability, activity, etc. (Boe-Hansen, Albrechtsen, Arvin, & Jorgensen, 2002) and may be very similar in terms of phylogeny, we decided to analyze the planktonic bacteria present in 31 sampling sites within a DWDS in order to characterize the bacterial community structures in these areas of the distribution system. Through analysis of bulk water samples, this study aims to identify and compare the microbial communities that are present in a DWDS fed by ground water and surface water. This study was conducted in order to gain a better understanding of the bacterial communities that are present within a municipal DWDS at Points of Use (POUs), with the focus of the study being a phylogenetic comparison between those communities found in different areas of the DWDS based on source. A total of 2786 16S rDNA clones were analyzed in this study. We found that Actinobacteria (mainly Mycobacterium sp.) and Alphaproteobacteria represented nearly 46% and 36% of the total clones examined, respectively. Other bacterial genera identified in this study include Betaproteobacteria, Gammaproteobacteria, Cyanobacteria, Firmicutes, Planctomycetes, and others. While Alphaproteobacteria has been shown to be a numerically dominant group in chlorinated drinking water system simulators (Williams, Domingo, Meckes, Kelty, & Rochon, 2004); (Williams, Santo Domingo, & Meckes, 2005), the surprising abundance of mycobacterial sequences recovered in this study indicate significant differences in microbial community structure between the WDS analyzed in this study as compared to others in the literature.

Download or read book American Doctoral Dissertations written by and published by . This book was released on 1997 with total page 806 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Novel Approaches to Analysing 16S Ribosomal RNA Gene Sequencing Profiles of the Gut Microbiota in Human Health Research written by Matthew Jackson and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: From this, I generate an index that can represent widescale gut microbiota composition within a single score that is also associated with host health. Secondly, I present a method to identify communities of interacting microbes within the gut microbiota and demonstrate that these reliably associate with host phenotypes across geographically diverse populations. In summary, this thesis addresses several issues currently associated with the analysis of 16S rRNA gene sequencing-based gut microbiota profiles and in turn identifies several novel biological phenomena. The techniques described herein should improve the utility of microbiota profiles derived from 16S rRNA gene sequencing and advance the field of gut microbiome research in human health.

Download or read book Identification of Bacteria with Ambiguous Biochemical Profiles by 16s Ribosomal RNA Gene Sequencing written by 陳賢良 and published by . This book was released on 2017-01-27 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Identification of Bacteria With Ambiguous Biochemical Profiles by 16S Ribosomal RNA Gene Sequencing" by 陳賢良, Yin-leung, Chan, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. DOI: 10.5353/th_b3197028 Subjects: Bacteria - Identification Nucleotide sequence Ribosomes

Download or read book The Prokaryotes written by Martin Dworkin and published by Springer. This book was released on 2006-12-13 with total page 959 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the launch of its first electronic edition, The Prokaryotes, the definitive reference on the biology of bacteria, enters an exciting new era of information delivery. Subscription-based access is available. The electronic version begins with an online implementation of the content found in the printed reference work, The Prokaryotes, Second Edition. The content is being fully updated over a five-year period until the work is completely revised. Thereafter, material will be continuously added to reflect developments in bacteriology. This online version features information retrieval functions and multimedia components.

Download or read book Applied Microbial Systematics written by Fergus Priest and published by Springer Science & Business Media. This book was released on 2000-11-30 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern approaches to microbial classification and identification, particularly those based on nucleic acid analysis, have raised the awareness and interest of microbiologists in systematics during the past decade. The extended scope of the subject has revolutionized microbial ecology with the demonstration of uncultivable microorganisms as a major component of the biosphere and evolution, with the ribosomal RNA phylogenetic tree as the basis of current classifications. However, advances in microbial systematics have also had enormous impact on other, diverse aspects of microbiology such as animal pathogenicity, plant-microbe interactions and relationships with food. In this book, we survey and discuss in depth the contribution of modern taxonomic approaches to our understanding of the microbiology of these various systems. The book does not concentrate on methods - these have been well reported elsewhere - instead it provides a unique insight into the application and value of modern systematics in diverse branches of microbiology. It will be of value to microbiologists at both research and technical levels who need to appreciate the range of organisms with which they work and the diversity within them. It will also be of value to teachers and students of microbiology courses who want to understand how systematics can enhance microbiology beyond the routine of classification, nomenclature, and identification.

Download or read book Bergey s Manual of Systematic Bacteriology written by David R. Boone and published by Springer Science & Business Media. This book was released on 2012-01-13 with total page 717 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacteriologists from all levels of expertise and within all specialties rely on this Manual as one of the most comprehensive and authoritative works. Since publication of the first edition of the Systematics, the field has undergone revolutionary changes, leading to a phylogenetic classification of prokaryotes based on sequencing of the small ribosomal subunit. The list of validly named species has more than doubled since publication of the first edition, and descriptions of over 2000 new and realigned species are included in this new edition along with more in-depth ecological information about individual taxa and extensive introductory essays by leading authorities in the field.

Download or read book The Prokaryotes written by Edward F. DeLong and published by Springer. This book was released on 2013-03-22 with total page 662 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Prokaryotes is a comprehensive, multi-authored, peer reviewed reference work on Bacteria and Achaea. This fourth edition of The Prokaryotes is organized to cover all taxonomic diversity, using the family level to delineate chapters. Different from other resources, this new Springer product includes not only taxonomy, but also prokaryotic biology and technology of taxa in a broad context. Technological aspects highlight the usefulness of prokaryotes in processes and products, including biocontrol agents and as genetics tools. The content of the expanded fourth edition is divided into two parts: Part 1 contains review chapters dealing with the most important general concepts in molecular, applied and general prokaryote biology; Part 2 describes the known properties of specific taxonomic groups. Two completely new sections have been added to Part 1: bacterial communities and human bacteriology. The bacterial communities section reflects the growing realization that studies on pure cultures of bacteria have led to an incomplete picture of the microbial world for two fundamental reasons: the vast majority of bacteria in soil, water and associated with biological tissues are currently not culturable, and that an understanding of microbial ecology requires knowledge on how different bacterial species interact with each other in their natural environment. The new section on human microbiology deals with bacteria associated with healthy humans and bacterial pathogenesis. Each of the major human diseases caused by bacteria is reviewed, from identifying the pathogens by classical clinical and non-culturing techniques to the biochemical mechanisms of the disease process. The 4th edition of The Prokaryotes is the most complete resource on the biology of prokaryotes. The following volumes are published consecutively within the 4th Edition: Prokaryotic Biology and Symbiotic Associations Prokaryotic Communities and Ecophysiology Prokaryotic Physiology and Biochemistry Applied Bacteriology and Biotechnology Human Microbiology Actinobacteria Firmicutes Alphaproteobacteria and Betaproteobacteria Gammaproteobacteria Deltaproteobacteria and Epsilonproteobacteria Other Major Lineages of Bacteria and the Archaea

Download or read book The Prokaryotes written by Martin Dworkin and published by Springer. This book was released on 2006-12-13 with total page 567 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the launch of its first electronic edition, The Prokaryotes, the definitive reference on the biology of bacteria, enters an exciting new era of information delivery. Subscription-based access is available. The electronic version begins with an online implementation of the content found in the printed reference work, The Prokaryotes, Second Edition. The content is being fully updated over a five-year period until the work is completely revised. Thereafter, material will be continuously added to reflect developments in bacteriology. This online version features information retrieval functions and multimedia components.

Download or read book Molecular Identification Systematics and Population Structure of Prokaryotes written by Erko Stackebrandt and published by Springer. This book was released on 2013-01-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Systematic biology has a far wider application than merely the provision of a reliable classification scheme for new strains. With the framework of the hierarchic system stabilizing, genomes, noncoding regions, and genes and their products can now be evaluated in an evolutionary context. This book summarizes recent developments in the molecular characterization of cultured and as-yet uncultured prokaryotes, emphasizing the strengths and weaknesses of individual approaches. The chapters of the book are compiled to stimulate students to enter the field of bacterial diversity, presenting a broad spectrum of fascinating multifaceted disciplines that illuminate the paths to ecosystem functioning, communication within communities, symbiosis, life in extreme environments, astrobiology, and more.

Download or read book Desk Encyclopedia of Microbiology written by Moselio Schaechter and published by Academic Press. This book was released on 2010-04-19 with total page 1277 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Desk Encyclopedia of Microbiology, Second Edition is a single-volume comprehensive guide to microbiology for the advanced reader. Derived from the six volume e-only Encyclopedia of Microbiology, Third Edition, it bridges the gap between introductory texts and specialized reviews. Covering topics ranging from the basic science of microbiology to the current "hot" topics in the field, it will be invaluable for obtaining background information on a broad range of microbiological topics, preparing lectures and preparing grant applications and reports. - The most comprehensive single-volume source providing an overview of microbiology to non-specialists - Bridges the gap between introductory texts and specialized reviews - Provides concise and general overviews of important topics within the field making it a helpful resource when preparing for lectures, writing reports, or drafting grant applications

Download or read book Extremophiles in Deep Sea Environments written by K. Horikoshi and published by Springer Science & Business Media. This book was released on 1999-11-01 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many organisms in deep-sea environments are extremophiles thriving in extreme conditions: high pressure, high or low temperature, or high concentrations of inorganic compounds. This book presents the microbiology of extremophiles living in the deep sea and describes the isolation, cultivation, and taxonomic identification of microorganisms retrieved from the Mariana Trench, the world's deepest point. Also explained are techniques for recovering pressure-loving bacteria, the barophiles (piezophiles), and for whole genome analysis of Bacillus halodurans C-125. Physiological analysis of the pressure effect in Saccharomyces cerevisiae and Escherichia coli is used to answer the question of how deep-sea organisms survive under high hydrostatic pressure. These research results are useful in both basic science and industrial applications. Readers discover a new microbial world in the ocean depths, with state-of-the-science information on extremophiles.

Download or read book Microbes and Microbial Technology written by Iqbal Ahmad and published by Springer Science & Business Media. This book was released on 2011-02-01 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on successful application of microbial biotechnology in areas such as medicine, agriculture, environment and human health.

Download or read book Essential Microbiology written by Stuart Hogg and published by John Wiley & Sons. This book was released on 2013-06-10 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: Essential Microbiology 2nd Edition is a fully revised comprehensive introductory text aimed at students taking a first course in the subject. It provides an ideal entry into the world of microorganisms, considering all aspects of their biology (structure, metabolism, genetics), and illustrates the remarkable diversity of microbial life by devoting a chapter to each of the main taxonomic groupings. The second part of the book introduces the reader to aspects of applied microbiology, exploring the involvement of microorganisms in areas as diverse as food and drink production, genetic engineering, global recycling systems and infectious disease. Essential Microbiology explains the key points of each topic but avoids overburdening the student with unnecessary detail. Now in full colour it makes extensive use of clear line diagrams to clarify sometimes difficult concepts or mechanisms. A companion web site includes further material including MCQs, enabling the student to assess their understanding of the main concepts that have been covered. This edition has been fully revised and updated to reflect the developments that have occurred in recent years and includes a completely new section devoted to medical microbiology. Students of any life science degree course will find this a concise and valuable introduction to microbiology.

Download or read book Bacterial Resistance to Antibiotics written by Boyan B. Bonev and published by John Wiley & Sons. This book was released on 2019-06-10 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: AN AUTHORITATIVE SURVEY OF CURRENT RESEARCH INTO CLINICALLY USEFUL CONVENTIONAL AND NONCONVENTIONAL ANTIBIOTIC THERAPEUTICS Pharmaceutically-active antibiotics revolutionized the treatment of infectious diseases, leading to decreased mortality and increased life expectancy. However, recent years have seen an alarming rise in the number and frequency of antibiotic-resistant "Superbugs." The Centers for Disease Control and Prevention (CDC) estimates that over two million antibiotic-resistant infections occur in the United States annually, resulting in approximately 23,000 deaths. Despite the danger to public health, a minimal number of new antibiotic drugs are currently in development or in clinical trials by major pharmaceutical companies. To prevent reverting back to the pre-antibiotic era—when diseases caused by parasites or infections were virtually untreatable and frequently resulted in death—new and innovative approaches are needed to combat the increasing resistance of pathogenic bacteria to antibiotics. Bacterial Resistance to Antibiotics – From Molecules to Man examines the current state and future direction of research into developing clinically-useful next-generation novel antibiotics. An internationally-recognized team of experts cover topics including glycopeptide antibiotic resistance, anti-tuberculosis agents, anti-virulence therapies, tetracyclines, the molecular and structural determinants of resistance, and more. Presents a multidisciplinary approach for the optimization of novel antibiotics for maximum potency, minimal toxicity, and appropriated degradability Highlights critical aspects that may relieve the problematic medical situation of antibiotic resistance Includes an overview of the genetic and molecular mechanisms of antibiotic resistance Addresses contemporary issues of global public health and longevity Includes full references, author remarks, and color illustrations, graphs, and charts Bacterial Resistance to Antibiotics – From Molecules to Man is a valuable source of up-to-date information for medical practitioners, researchers, academics, and professionals in public health, pharmaceuticals, microbiology, and related fields.

Download or read book The Flagellar World written by Shin-Ichi Aizawa and published by Elsevier. This book was released on 2013-12-16 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Flagellar World is a unique publication. The product of years of research and data collection by the author, this book is a pictorial guide to flagella in a variety of organisms. Each EM image is accompanied by a short description of the system in each organism. These never-before-seen pictures represent a wide variety of flagella, including Legionella pneumophila, Escherichia coli, Pseudomonas aeruginosa, and many others. Researchers in microbiology, immunology, and parasitology will find this a fascinating and useful resource. - A unique publication with many never before seen EM images of flagella - A historical document of years of research on flagella - Fills a specific niche that shows flagella in several varied organisms