Download or read book NUCLEAR MAGNETIC RESONANCE PROBES OF MEMBRANE BIOPHYSICS STRUCTURE AND DYNAMICS written by and published by . This book was released on 2010 with total page 688 pages. Available in PDF, EPUB and Kindle. Book excerpt: The phospholipid membrane is a self-assembled, dynamic molecular system that may exist alone in association with only water, or in complex systems comprised of multiple lipid types and proteins. In this dissertation the intra- and inter-molecular forces responsible for the atomistic, molecular and collective equilibrium structure and dynamics are studied by nuclear magnetic resonance spectroscopy (NMR). The multi-nuclear NMR measurements and various experimental techniques are able to provide data that enable the characterization of the hierarchical spatio-temporal organization of the phospholipid membrane. The experimental and theoretical studies conducted target membrane interactions ranging from model systems composed of only water and lipids, to multiple component domain forming membranes that are in association with peripheral and trans-membrane proteins. These measurements consisit of frequency spectrum lineshapes and nuclear-spin relaxation rates obtained using2H NMR, 13C NMR, 31P NMR and1H NMR. The changes of these experimental observables are interpreted within a statistical thermodynamic framework that allows the membrane structure, activation energies, and correlation times of motion to be determined. The cases presented demonstrate how fundamental principles of NMR spectroscopy may be applied to a host of membranes, leading to the biophysical characterization of membrane structure and dynamics.
Download or read book Nuclear Magnetic Resonance Probes of Molecular Dynamics written by R. Tycko and published by Springer Science & Business Media. This book was released on 2003-07-31 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nuclear Magnetic Resonance Probes of Molecular Dynamics describes the theoretical basis and experimental techniques that make modern NMR spectroscopy a powerful and flexible tool for probing molecular dynamics in chemical, physical, and biochemical systems. Individual chapters, written by leaders in the development and application of NMR from around the world, treat systems that range from synthetic polymers, liquid crystals, and catalysts to proteins and oligonucleotides and techniques that include deuterium NMR, magic angle spinning, multidimensional spectroscopy, and magnetic resonance imaging. A combination of elementary and advanced material makes the book a useful introduction to the field for students at the graduate level as well as an important reference for practising NMR spectroscopists.
Download or read book Biological Magnetic Resonance written by Lawrence J. Berliner and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt: We are pleased to present Volume 9 of our highly successful series, which now celebrates 12 years of providing the magnetic resonance community with topical, authoritative chapters on new aspects of biological magnetic resonance. As always, we try to present a diversity of topic coverage in each volume, ranging from applications of in vivo magnetic resonance to more fundamental aspects of electron spin resonance and nuclear magnetic resonance. Philip Yeagle presents an eagerly awaited chapter on 31p NMR studies of membranes and membrane protein interactions. Alan Marshall has con tributed two chapters to the volume: one, with Jiejun Wu, describes magnetic resonance studies of 5S-RNA as probes of its structure and conformation; the secon
Download or read book NMR of Proteins and Small Biomolecules written by Guang Zhu and published by Springer Science & Business Media. This book was released on 2012-03-28 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt: Application of NMR and Molecular Docking in Structure-Based Drug Discovery, by Jaime L. Stark and Robert Powers NMR as a Unique Tool in Assessment and Complex Determination of Weak Protein-Protein Interactions, by Olga Vinogradova and Jun Qin The Use of Residual Dipolar Coupling in Studying Proteins by NMR, by Kang Chen und Nico Tjandra NMR Studies of Metalloproteins, by Hongyan Li and Hongzhe Sun Recent Developments in 15N NMR Relaxation Studies that Probe Protein Backbone Dynamics, by Rieko Ishima Contemporary Methods in Structure Determination of Membrane Proteins by Solution NMR, by Tabussom Qureshi and Natalie K. Goto Protein Structure Determination by Solid-State NMR, by Xin Zhao Dynamic Nuclear Polarization: New Methodology and Applications, by Kong Hung Sze, Qinglin Wu, Ho Sum Tse and Guang Zhu
Download or read book Biological NMR Spectroscopy written by John L. Markley and published by Oxford University Press. This book was released on 1997-01-30 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a critical assessment of progress on the use of nuclear magnetic resonance spectroscopy to determine the structure of proteins, including brief reviews of the history of the field along with coverage of current clinical and in vivo applications. The book, in honor of Oleg Jardetsky, one of the pioneers of the field, is edited by two of the most highly respected investigators using NMR, and features contributions by most of the leading workers in the field. It will be valued as a landmark publication that presents the state-of-the-art perspectives regarding one of today's most important technologies.
Download or read book Biological NMR Part B written by and published by Academic Press. This book was released on 2019-01-10 with total page 546 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological NMR, Part B, the latest release in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Protein methyl labeling, Membrane protein expression – yeast, Protein aromatic labeling, His-tag/Metal contamination, Bicelles, nanodiscs & micelles MP host, PTM – phosphorylation, PTM – lipidation, Screening platform for receptor-ligand discovery, Solution Spectroscopy, Large protein strategies, NUS data collection/analysis, F19 incl. hydration, ODNP - hydration, Reverse micelle - Hydration, Solid State Spectroscopy, SS NMR membrane proteins, SS NMR soluble/aggregate proteins, SS DNP - general, SS NMR nucleic acids, and much more. - Authoritative contributors - Protocols for state-of-the-art advances - Timeliness
Download or read book The Chemistry of Hyperpolarized Magnetic Resonance Probes written by Eul Hyun Suh and published by Academic Press. This book was released on 2024-06-01 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Chemistry of Hyperpolarized Magnetic Resonance Probes, Volume Seven focuses on the chemical aspects of hyperpolarized NMR/MRI technology, with synthesis and characterizations of labeled compounds discussed from a practical point-of-view. A brief overview of the various hyperpolarization techniques are given, with the optimization of hyperpolarization conditions and the determination of critical parameters such as polarization level and T1 relaxation values described. A practical guide on the in vivo applications of hyperpolarized compounds in small animals is also included. - Helps readers understand the structural features that determine the properties of HP-probes, such as chemical shift and relaxation times - Aids readers in selecting stable isotope labeled probes for hyperpolarized NMR/MRI applications - Teachers readers how to use the most appropriate synthetic methodology for the labeled probes - Covers how to find the most suitable polarization technique (DNP, PHIP etc.) for the probe
Download or read book Dynamic Hyperpolarized Nuclear Magnetic Resonance written by Thomas Jue and published by Springer. This book was released on 2021-06-24 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book in the series to focus on dynamic hyperpolarized nuclear magnetic resonance, a burgeoning topic in biophysics. The volume follows the format and style of the Handbook of Modern Biophysics series and expands on topics already discussed in previous volumes. It builds a theoretical and experimental framework for students and researchers who wish to investigate the biophysics and biomedical application of dynamic hyperpolarized NMR. All contributors are internationally recognized experts, lead the dynamic hyperpolarized NMR field, and have first-hand knowledge of the chapter material. The book covers the following topics: Hyperpolarization by dissolution Dynamic Nuclear Polarization Design considerations for implementing a hyperpolarizer Chemical Shift Imaging with Dynamic Hyperpolarized NMR Signal Sampling Strategies in Dynamic Hyperpolarized NMR Kinetic Modeling of Enzymatic Reactions in Analyzing Hyperpolarized NMR Data Using Hyperpolarized NMR to Understand Biochemistry from Cells to Humans Innovating Metabolic Biomarkers for Hyperpolarized NMR New Insights into Metabolic Regulation from Hyperpolarized 13C MRS/MRI Studies Novel Views on Heart Function from Dynamic Hyperpolarized NMR Insights on Lactate Metabolism in Skeletal Muscle based on 13C Dynamic Nuclear Polarization Studies About the Editors Dirk Mayer is Professor of Diagnostic Radiology and Nuclear Medicine at the University of Maryland and is the Director of Metabolic Imaging. He is a recognized expert on dynamic nuclear polarization (DNP) MRI-based imaging techniques and has optimized acquisition and reconstruction techniques, has constructed kinetic modeling for quantitative analysis, and has developing new probes. Thomas Jue is Professor of Biochemistry and Molecular Medicine at the University of California Davis. He is an internationally recognized expert in developing and applying magnetic resonance techniques to study animal as well as human physiology in vivo. He served as a Chair of the Biophysics Graduate Group Program at UC Davis, where he started to redesign a graduate curriculum that balances physical science/mathematics formalism and biomedical perspective in order to promote interest at the interface of physical science, engineering, mathematics, biology, and medicine. The Handbook of Modern Biophysics represents an aspect of that effort.
Download or read book NMR Spectroscopy for Probing Functional Dynamics at Biological Interfaces written by Anirban Bhunia and published by Royal Society of Chemistry. This book was released on 2022-08-17 with total page 733 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing a comprehensive amalgamation of the scattered knowledge of how to apply high-resolution NMR techniques to biomolecular systems, this book will break down the conventional stereotypes in the use of NMR for structural studies.
Download or read book Modern Techniques in Protein NMR written by N. Rama Krishna and published by Springer. This book was released on 1999-04-30 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 16 marks the beginning of a special topic series devoted to modern techniques in protein NMR, under the Biological Magnetic Resonance series. This volume is being followed by Volume 17 with the subtitle Structure Computation and Dynamics in Protein NMR. Volumes 16 and 17 present some of the recent, significant advances in biomolecular NMR field with emphasis on developments during the last five years. We are honored to have brought together in these volumes some of the world’s foremost experts who have provided broad leadership in advancing this field. Volume 16 contains advances in two broad categories: the first, Large Proteins, Complexes, and Membrane Proteins, and second, Pulse Methods. Volume 17, which will follow covers major advances in Computational Methods, and Structure and Dynamics. In the opening chapter of Volume 16, Marius Clore and Angela Gronenborn give a brief review of NMR strategies including the use of long range restraints in the structure determination of large proteins and protein complexes. In the next two chapters, Lewis Kay and Ron Venters and their collaborators describe state-of-t- art advances in the study of perdeuterated large proteins. They are followed by Stanley Opella and co-workers who present recent developments in the study of membrane proteins. (A related topic dealing with magnetic field induced residual dipolar couplings in proteins will appear in the section on Structure and Dynamics in Volume 17).
Download or read book The Application of Nuclear Magnetic Resonance to Molecules Undergoing Rapid Axial Reorientation written by Ivana Komljenovic and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The cell membrane is critical for life. The functionality of this membrane is dependent on the interactions between the many different molecules found within and connected to the cell membrane. Proteins and peptides are responsible for a variety of biological activities including signal transmission and ion movement. Membrane proteins can be associated very closely with the membrane (known as integral membrane proteins) or loosely with the membrane (peripheral proteins). The functionality of these membrane proteins depends on their structure, which can be studied using nuclear magnetic resonance (NMR). In structural studies, protons present themselves as desirable nuclei for detection, owing to their high abundance in biological systems. However, strong homonuclear dipolar interactions often lead to broad signals and obscured details. This thesis describes the study and development of NMR pulse sequences that can be used to investigate model membrane systems that include small molecules. Beginning with a leucine-rich peptide P0813, proton suppression techniques were developed using a unique gradient probe assembly. The addition of pulsed field gradients within the pulse sequences resulted in significant proton coherence suppression in a sample composed of protonated lipid and peptide. Gramicidin-A was used in the development of proton-detected correlation experiments. These experiments were further studied with Conolysin-Mt1, a natural cytolytic peptide found in sea snails. Cholesterol was employed in the study of how the nuclear Overhauser effect can be used to probe molecular structure and dynamics. Finally, the majority of the modified experiments were tested on a synthetic peptide ALGA, which was originally designed to produce well-resolved proton spectra. Throughout the studies performed on the five different small molecules, new proton detection techniques were investigated, with varying degrees of success. In addition, different sample preparations were explored to help determine how changes in sample composition can affect sample stability.
Download or read book Ab Initio and Nuclear Magnetic Resonance Probes of Molecular Structure and Dynamics written by Ryan Andrew Olsen and published by . This book was released on 2005 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book ESR Spectroscopy in Membrane Biophysics written by Marcus A. Hemminga and published by Springer Science & Business Media. This book was released on 2007-02-13 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Starting from a comprehensive quantum mechanical description, this book introduces the optical (IR, Raman, UV/Vis, CD, fluorescence and laser spectroscopy) and magnetic resonance (1D and 2D-NMR, ESR) techniques. The book offers a timely review of the increasing interest in using spin-label ESR as an alternative structural technique for NMR or X-ray diffraction. Future aspects are treated as well, but only as an illustration of the progress of ESR in this field.
Download or read book Nmr Probeheads For Biophysical And Biomedical Experiments Theoretical Principles And Practical Guidelines 2nd Edition written by Joel Mispelter and published by World Scientific Publishing Company. This book was released on 2015-05-27 with total page 754 pages. Available in PDF, EPUB and Kindle. Book excerpt: NMR Probeheads for Biophysical and Biomedical Experiments 2nd Edition is essential reading for anyone in the field of NMR or MRI, from students to medical or biological scientists performing experiments under certain physical and/or geometrical conditions, unattainable by conventional or available probes. The material guides the reader through the most basic and comprehensive stages in accomplishing a correct probe design, from a very basic oscillating circuit to much more elaborate designs.This new edition has been revised and updated to include a chapter dedicated to RF components, which are commonly used for probes realization and their frequency-dependent characteristics. Another completely revised chapter concerns the multiple coil systems and discusses arrays coils, different decoupling methods, and some principles for interfacing coils with low-noise preamplifiers. The principles of linear circuit analysis are presented in a dedicated chapter. Last but not least, accompanying files containing updated software for probe design have been made available from the publisher's website.
Download or read book Comprehensive Biophysics written by and published by Academic Press. This book was released on 2012-04-12 with total page 3533 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biophysics is a rapidly-evolving interdisciplinary science that applies theories and methods of the physical sciences to questions of biology. Biophysics encompasses many disciplines, including physics, chemistry, mathematics, biology, biochemistry, medicine, pharmacology, physiology, and neuroscience, and it is essential that scientists working in these varied fields are able to understand each other's research. Comprehensive Biophysics, Nine Volume Set will help bridge that communication gap. Written by a team of researchers at the forefront of their respective fields, under the guidance of Chief Editor Edward Egelman, Comprehensive Biophysics, Nine Volume Set provides definitive introductions to a broad array of topics, uniting different areas of biophysics research - from the physical techniques for studying macromolecular structure to protein folding, muscle and molecular motors, cell biophysics, bioenergetics and more. The result is this comprehensive scientific resource - a valuable tool both for helping researchers come to grips quickly with material from related biophysics fields outside their areas of expertise, and for reinforcing their existing knowledge. Biophysical research today encompasses many areas of biology. These studies do not necessarily share a unique identifying factor. This work unites the different areas of research and allows users, regardless of their background, to navigate through the most essential concepts with ease, saving them time and vastly improving their understanding The field of biophysics counts several journals that are directly and indirectly concerned with the field. There is no reference work that encompasses the entire field and unites the different areas of research through deep foundational reviews. Comprehensive Biophysics fills this vacuum, being a definitive work on biophysics. It will help users apply context to the diverse journal literature offering, and aid them in identifying areas for further research Chief Editor Edward Egelman (E-I-C, Biophysical Journal) has assembled an impressive, world-class team of Volume Editors and Contributing Authors. Each chapter has been painstakingly reviewed and checked for consistent high quality. The result is an authoritative overview which ties the literature together and provides the user with a reliable background information and citation resource
Download or read book Spin Label Electron Paramagnetic Resonance Spectroscopy written by Derek Marsh and published by CRC Press. This book was released on 2019-12-09 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: Spin-label electron paramagnetic resonance (EPR) spectroscopy is a versatile molecular probe method that finds wide application in molecular biophysics and structural biology. This book provides the first comprehensive summary of basic principles, spectroscopic properties, and use for studying biological membranes, protein folding, supramolecular structure, lipid-protein interactions, and dynamics. The contents begin with discussion of fundamental theory and practice, including static spectral parameters and conventional continuous-wave (CW) spectroscopy. The development then progresses, via nonlinear CW-EPR for slower motions, to the more demanding time-resolved pulse EPR, and includes an in-depth treatment of spin relaxation and spectral line shapes. Once the spectroscopic fundamentals are established, the final chapters acquire a more applied character. Extensive appendices at the end of the book provide detailed summaries of key concepts in magnetic resonance and chemical physics for the student reader and experienced practitioner alike. Key Features: Indispensable reference source for the understanding and interpretation of spin-label spectroscopic data in its different aspects. Tables of fundamental spectral parameters are included throughout. Forms the basis for an EPR graduate course, extending up to a thorough coverage of advanced topics in Specialist Appendices. Includes all necessary theoretical background. The primary audience is research workers in the fields of molecular biophysics, structural biology, biophysical chemistry, physical biochemistry and molecular biomedicine. Also, physical chemists, polymer physicists, and liquid-crystal researchers will benefit from this book, although illustrative examples used are often taken from the biomolecular field. Readers will be postgraduate researchers and above, but include those from other disciplines who seek to understand the primary spin-label EPR literature.
Download or read book Membrane Protein Structure Determination written by Jean-Jacques Lacapère and published by Methods in Molecular Biology. This book was released on 2010-08-06 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Membrane proteins, representing nearly 40% of all proteins, are key components of cells involved in many cellular processes, yet only a small number of their structures have been determined. Membrane Protein Structure Determination: Methods and Protocols presents many detailed techniques for membrane protein structure determination used today by bringing together contributions from top experts in the field. Divided into five convenient sections, the book covers various strategies to purify membrane proteins, approaches to get three dimensional crystals and solve the structure by x-ray diffraction, possibilities to gain structural information for a membrane protein using electron microscopy observations, recent advances in nuclear magnetic resonance (NMR), and molecular modelling strategies that can be used either to get membrane protein structures or to move from atomic structure to a dynamic understanding of a molecular functioning mechanism. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Membrane Protein Structure Determination: Methods and Protocols serves as an ideal reference for scientists seeking to further our knowledge of these vital and versatile proteins as well as our overall understanding of the complicated world of cell biology.
