Download or read book Nouvelle technique d optimisation de la d tection mol culaire base d un spectrom tre microscope FTIR written by Hamza Landari and published by . This book was released on 2021 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt: L’identification et la quantification des espèces transmise aux cellules nerveuses lors des échanges moléculaires entre les neurones sous la forme de neurotransmetteurs nous permettent de mieux comprendre certains fonctionnements du cerveau et certaines maladies neurodégénératives. À son tour, cette compréhension nous permettrait à long terme d’améliorer la qualité de vie des patients souffrants de ces maladies. D’un autre côté, la composition des aliments sous la forme de valeurs nutritionnelles nous permet de mieux connaître l’effet de la consommation de ces aliments sur la santé afin d’éviter de graves complications. De plus, la connaissance de cette composition nous permet d’améliorer la qualité de ces produits. Pour extraire la composition et la concentration des neurotransmetteurs dans le cerveau ou les valeurs nutritionnelles des aliments, il faut développer des outils d’analyse chimique et moléculaire très précise et sélective. L’analyse chimique d’un échantillon biologique nécessite deux étapes : (1)l’échantillonnage du liquide biologique et (2) l’étude de la composition de l’échantillon. Cette dernière étape consiste à identifier/quantifier la composition moléculaire des échantillons et à caractériser les propriétés chimiques de ces derniers. Cette étape nécessite donc le développement des instruments et des algorithmes de détection moléculaire avancés qui permettent de déterminer les concentrations physiologiques des neurotransmetteurs et d’autres molécules biologiques. Dans cette étude, nous nous sommes intéressés aux neurotransmetteurs suivants: dopamine hydrochloride, acide L-ascorbique, acétylcholine chloride, y-aminobutyrique, glycineet acide L-glutamique et aux molécules de sucre, incluant le glucose, le fructose et le saccharose. Le choix de l’instrument à utiliser dépend de plusieurs critères, dont les caractéristiques del’échantillon (viscosité, volume parmi d’autres). Dans le cadre de cette thèse de doctorat, nous examinons les performances de plusieurs méthodes et instruments de détection, soit l’électrochimie, la spectroscopie d’impédance et la spectroscopie UV/Visible et infrarouge (IR), pour la détection de différents types de molécules dans des échantillons aqueux. Par la suite, nous proposons une nouvelle approche d’analyse des échantillons aqueux basée sur la spectroscopieI R. Nous nous intéressons plus particulièrement au spectromètre microscope-FTIR qui nous permet d’effectuer des analyses spectroscopiques IR et de visualiser la distribution spatiale et moléculaire dans l’échantillon. Cependant, le spectromètre microscope-FTIR, dans sa configuration standard, ne permet pas l’analyse des échantillons aqueux à cause de la forte absorption des rayonnements IR par les molécules d’eau. Pour résoudre ce problème, nous avons proposé un nouveau système microfluidique de contrôle et de manipulation des liquides qui a été intégré au spectromètre microscope-FTIR pour analyser des échantillons aqueux. Le nouveau système proposé consiste en un module de réflexion, une couche hydrophobe et un module microfluidique. Le module de réflexion est maintenu à une température élevée pour l’évaporation rapide du solvant. La couche hydrophobe permet à son tour de confiner l’échantillon en un espace restreint pour avoir une distribution plus uniforme des molécules sur la surface du substrat. Ce nouveau système nous a permis d’identifier et de quantifier différents types de molécules tels que les neurotransmetteurs et les molécules de sucres, dont le glucose, le fructose et le saccharose. La limite de détection que nous avons pu atteindre avec notre système est de 10 μMolaire pour les neurotransmetteurs, de 3 mMolaire pour le glucose et le fructose et de 1 mMolaire pour le saccharose. En plus, nous avons proposé un nouvel algorithme pourune identification automatique des neurotransmetteurs par le microscope FTIR. À travers cetalgorithme, nous avons pu identifier, dans des solutions synthétiques (homogènes et hétérogènes), les six neurotransmetteurs suivants : la dopamine hydrochloride, l’acide L-ascorbique,l’acétylcholine chloride, l’y-aminobutyrique, la glycine et l’acide L-glutamique. La précision de la détection du nouvel algorithme est de 75% à 100% pour les six neurotransmetteurs que nous avons étudiés dans 36 échantillons homogènes et 45 échantillons hétérogènes.

Download or read book Pattern Recognition and Machine Learning written by Christopher M. Bishop and published by Springer. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first textbook on pattern recognition to present the Bayesian viewpoint. The book presents approximate inference algorithms that permit fast approximate answers in situations where exact answers are not feasible. It uses graphical models to describe probability distributions when no other books apply graphical models to machine learning. No previous knowledge of pattern recognition or machine learning concepts is assumed. Familiarity with multivariate calculus and basic linear algebra is required, and some experience in the use of probabilities would be helpful though not essential as the book includes a self-contained introduction to basic probability theory.

Download or read book Chronobiotechnology and Chronobiological Engineering written by L.E. Scheving and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 475 pages. Available in PDF, EPUB and Kindle. Book excerpt: High blood pressure (BP) (with fats and smoking) is one of the three roots of cardio-cerebro-renovascular disease affecting up to 25% of the adult population. Hence, high blood pressure should be recognized and treated, to reduce any complications and prolong life, as noted by Michael Weber of the Veterans Administration Hospital in Long Beach, California. He further emphasizes the need for monitoring before one starts the treatment of high blood pressure. Indeed, he refers to the results of the Australian study on mild hypertension with a large percentage of placebo responders and rightly suggests that many people are treated who should not be because of 'white-coat-associated high blood pressure'. He also points to the lack of standardization of techniques for data analysis and of methods of BP measurement. Ambulatory monitoring under usual condi tions without concomitant recording of events does not allow even a qualitative assessment of the impact of varying stimuli, in weber's opinion.

Download or read book Portable Life Support Systems written by and published by . This book was released on 1970 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Ocular Surface Disease written by Ali R. Djalilian and published by Springer. This book was released on 2017-11-16 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: Using a case-based format to help disseminate some of the hard-to-find information on ocular surface disease, this book is written and edited by leaders in this rapidly evolving field. Each chapter is built around three actual patient cases with varying degrees of complexity whereby detailed aspects of the care are described in order to provide practical information and illustrate real world challenges. Ocular Surface Disease covers the most common disorders, ranging from dry eye disease and tear film insufficiency to the more severe cicatricial diseases. The role and application of both medical and surgical treatments are carefully explained. The book is intended for ophthalmologists, both in practice or in training, who are seeking an update on the more advanced clinical practices

Download or read book Space Biology and Medicine written by Arnauld E. Nicogossian and published by AIAA (American Institute of Aeronautics & Astronautics). This book was released on 1993 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume II in the Space Biology and Medicine series, Life Support and Habitability addresses major issues and requirements for safe habitability and work beyond the Earths atmosphere. It is composed of two parts: The Spacecraft Environment and Life Support Systems. As in the first volume, the authors are specialists in their fields in the United States and Russian Federation. The book is intended for a widespread audience. In particular, it will appeal to students majoring in biomedical and technical subjects who intend to specialize in space science, engineers developing life support systems, and physicians and scientists formulating medical specifications for habitability conditions onboard spacecraft and monitoring compliance with them. There are more than 285 figures and tables and 1100 references.

Download or read book Forensic Science Strategy written by Great Britain. Home Office and published by . This book was released on 2016 with total page 26 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Lipid Analysis written by W. W. Christie and published by Elsevier. This book was released on 2010-01-10 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: This well-known and highly successful book was first published in 1973 and has been completely re-written in subsequent editions (published in 1982 and 2003). This new Fourth Edition has become necessary because of the pace of developments in mass spectrometry of intact lipids, which has given recognition of lipid analysis and ‘lipidomics’ as a distinct science. To bring the book up to date with these developments, author William W. Christie is joined by co-author Xianlin Han. Although devoting considerable space to mass spectrometry and lipidomics, Lipid analysis remains a practical guide, in one volume, to the complexities of the analysis of lipids. As in past editions, it is designed to act as a primary source, of value at the laboratory bench rather than residing on a library shelf. Lipid analysis deals with the isolation, separation, identification and structural analysis of glycerolipids, including triacylglycerols, phospholipids, sphingolipids, and the various hydrolysis products of these. The chapters follow a logical sequence from the extraction of lipids to the isolation and characterization of particular lipid classes and of molecular species of each, and to the mass spectrometric analysis of lipids and lipidomics. The new influence of mass spectrometry is due mainly to the development of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). Most emphasis in this book is placed on ESI, which is enabling structural characterization of different lipid classes and the identification of novel lipids and their molecular species.

Download or read book Capillary Electrophoresis Mass Spectrometry CE MS written by Gerhardus de Jong and published by John Wiley & Sons. This book was released on 2016-09-13 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diese Monographie bietet einen vollständigen Überblick über die Prinzipien und Anwendungen der Kapillarelektrophorese (CE) und der Massenspektrometrie (MS) und legt den Nachdruck insbesondere auf Kopplungsschnittstellen. Ausführlich erläutert werden auch alle relevanten Substanzklassen. Ein einzigartiges Wissenskompendium für alle, die sich CE-MS beschäftigen!

Download or read book Handbook of Nutrition and Food written by Carolyn D. Berdanier and published by CRC Press. This book was released on 2016-04-19 with total page 1136 pages. Available in PDF, EPUB and Kindle. Book excerpt: The new edition of the Handbook of Nutrition and Food follows the format of the bestselling earlier editions, providing a reference guide for many of the issues on health and well being that are affected by nutrition. Completely revised, the third edition contains 20 new chapters, 50 percent new figures, and updates to most of the previously existi

Download or read book Handbook of Clinical Nanomedicine written by Raj Bawa and published by Pan Stanford. This book was released on 2015-07-31 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The exciting advances in nanomedicine in the past two decades highlight the growing need for an authoritative and comprehensive reference that can be relied upon by scientists, clinicians, academics, industry, students, lawyers, and policy-makers alike. With this in mind, the Handbook of Clinical Nanomedicine: Law, Business, Regulation, Safety, and Risk and its related companion volumes in the series aim to provide a broad survey of interconnected topics pertaining to clinical nanomedicine. This is the second volume in the series. Each volume is intended to be a stand‐alone reference source, presented in a user-friendly format for easy access. This volume examines nanomedical products’ entire "product life cycle" from the creation of nanomedical products to the final market introduction. It not only provides a comprehensive roadmap of basic and clinical research but also tackles critical topics such as regulatory science, intellectual property law, risk analysis, toxicology, nano-characterization, and commercialization activities. All chapters contain keywords, figures in full color, author biographies, and an extensive list of references. The handbook is truly essential reading for the novice and the expert alike in fields such as medicine, law, biotechnology, pharmaceutical sciences, engineering, policy, future studies, ethics, licensing, and toxicology, among others. While bridging the gap between basic biomedical research, engineering, medicine, law, and commercialization, the handbook allows readers to gain a thorough understanding of nano’s potential to address medical problems from both the patient and the health provider’s point of view; current applications and potential in a healthcare setting; regulatory, environment, and health issues; and intellectual property, licensing, and commercialization issues as well as business considerations. The editor and assistant editors have skillfully curated each chapter to reflect the most relevant and current information possible. The range of topics covered as well as the diverse selection of distinguished authors is truly impressive. The multidisciplinary approach of this handbook as well as its focus on not only scientific and technical aspects of nanomedicine but also legal, regulatory, policy, and commercial aspects will attract a global audience. In short, the Handbook of Clinical Nanomedicine: Law, Business, Regulation, Safety, and Risk promises to be a standard reference for any serious practitioner in this expansive field.

Download or read book The Parahippocampal Region written by Menno P. Witter and published by Oxford University Press, USA. This book was released on 2002 with total page 347 pages. Available in PDF, EPUB and Kindle. Book excerpt: The recent dramatic advances in imaging technologies have enabled researchers to build on the evidence obtained from lesion and behavioural studies to propose a new range of functions for the parahippocampal region. It is now possible to image the region in healthy human subjects and todefine the pathological changes occurring during the early phases of a range of neurologic and psychiatric conditions.The results have uncovered evidence suggesting that the region plays an important role in the higher cognitive processes of learning and memory, and in specific brain diseases, including Alzheimer's disease, schizophrenia and epilepsy, as well as in the aging process itself.The Parahippocampal Region: Organization and Role in Cognitive Functions examines the architecture and activity of this section of the temporal lobe, describes the systems active in memory, perception and behaviour, and outlines the significance of its involvement in the progress of a range ofdisease states. It provides an overview of our current basic and clinical knowledge and a baseline for further expansion of the functional understanding of the region.With contributions from an international team of authors this book will be of interest to neuroscientists, researchers and practitioners from the fields of neuropsychology, neurology and psychiatry, as well as to students from these disciplines.

Download or read book Advancements in Teleoperator Systems written by and published by . This book was released on 1970 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Introduction to Human Nutrition written by Michael J. Gibney and published by John Wiley & Sons. This book was released on 2013-03-14 with total page 740 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this Second Edition of the introductory text in the acclaimed Nutrition Society Textbook Series, Introduction to Human Nutrition has been revised and updated to meet the needs of the contemporary student. Groundbreaking in their scope and approach, the titles in the series: Provide students with the required scientific basics of nutrition in the context of a systems and health approach Enable teachers and students to explore the core principles of nutrition, to apply these throughout their training, and to foster critical thinking at all times. Throughout, key areas of knowledge are identified Are fully peer reviewed, to ensure completeness and clarity of content, as well as to ensure that each book takes a global perspective Introduction to Human Nutrition is an essential purchase for undergraduate and postgraduate students of nutrition/nutrition and dietetics degrees, and also for those students who major in other subjects that have a nutrition component, such as food science, medicine, pharmacy and nursing. Professionals in nutrition, dietetics, food science, medicine, health sciences and many related areas will also find much of great value within this book.

Download or read book Trichier written by Alessandra Ceretto and published by Lulu.com. This book was released on with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Ultrasonography of the Eye and Orbit written by D. Jackson Coleman and published by Lww. This book was released on 2006 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by well-known leaders in ophthalmic ultrasonography, this volume is a complete guide to the use of ultrasound as a primary diagnostic tool in ophthalmology. This thoroughly revised Second Edition reflects the latest developments in three-dimensional ultrasound and other advanced technologies and the expanding clinical role of ultrasound, including its use in refractive surgery, post-LASIK evaluation, and neuro-ophthalmology. Coverage includes detailed chapters on ocular diagnosis, orbital diagnosis, and very high-frequency digital ultrasound scanning in LASIK and phakic intraocular lenses. More than 200 full-color, two-color, and black-and-white illustrations complement the text. A bound-in DVD contains video clips of patient ultrasound examinations.

Download or read book Capillary Electrophoresis of Proteins and Peptides written by Nguyet Thuy Tran and published by Humana. This book was released on 2016-07-30 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and exhaustive, Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols serves both beginners and experts with a collection of the current and most active topics in this vital field of study.