Download or read book Nonlinear Optical Microscopy for Tissue Imaging and Analysis in Anatomic Pathology written by Kseniya S. Shin and published by . This book was released on 2020 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stimulated Raman Scattering (SRS) microscopy provides chemical information for imaged tissue at submicron resolution. In the experiments described, I combine SRS with other non-linear microscopies, including second harmonic generation (SHG) microscopy and two-photon fluorescence (TPF) microscopy, to develop a methodology for tissue imaging and analysis with anatomic pathology diagnostic challenges in mind. Employing an interdisciplinary approach in collaboration with experts in the field I investigate the diagnostic potential of a multimodal approach to complex tissues such as brain, breast, and bone, illuminating the immense potential non-linear optical microscopy has to offer to pathologists and clinicians as they strive to improve patient care.

Download or read book Multiphoton Microscopy and Fluorescence Lifetime Imaging written by Karsten König and published by Walter de Gruyter GmbH & Co KG. This book was released on 2018-01-22 with total page 785 pages. Available in PDF, EPUB and Kindle. Book excerpt: This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue imaging Multiphoton Tomography (MPT) Clinical multimodal CARS imaging In vivo multiphoton microscopy of human skin Two-photon microscopy and fluorescence lifetime imaging of the cornea Multiscale correlative imaging of the brain Revealing interaction of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM in cosmetic clinical research Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models Bedside assessment of multiphoton tomography

Download or read book Nonlinear Microscopy for Histology written by Adam Tuer and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Handbook of Biomedical Nonlinear Optical Microscopy written by Barry R. Masters and published by Oxford University Press. This book was released on 2008-05-19 with total page 895 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ideal for cell biologists, life scientists, biomedical engineers, and clinicians, this handbook provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy.

Download or read book Image Analysis and Pattern Recognition for Automated Classification of Tissue Samples by Means of Nonlinear Optical Microscopy written by Anna Medyukhina and published by . This book was released on 2014 with total page 135 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Microscopy Techniques for Biomedical Education and Healthcare Practice written by Leonard Shapiro and published by Springer Nature. This book was released on 2023-11-02 with total page 235 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edited book has a strong focus on advances in microscopy that straddles research, medical education and clinical practice. These advances include the shift in power from conventional to digital microscopy. The first section of this book covers imaging techniques and morphometric image analysis with its applications in biomedicine using different microscopy modes. Chapters highlight the rich development of fluorescence methods and technologies; particle tracking techniques with applications in biomedical research and nanomedicine; the way in which visualizations have revolutionized taxonomy from gross anatomy to genetics; and the psychology of perception and how it affects our understanding of cells and tissues. The book’s first section concludes by exploring the use of CT modalities to evaluate anterior deformities in craniosynostosis. In the second section of the book, chapters on anatomical and cell biology education explore the history of anatomical models and their use in educational settings. This includes examples in 3D printing and functional human anatomical models that can be created using easily available resources and the use of biomedical imaging in visuospatial teaching of anatomy; the novel use of ultrasound in medical education and practice; and skill acquisition in histology education using a flowchart called a ‘decision tree’. This book will appeal to histologists, microscopists, cell biologists, clinicians and those involved in anatomical education and biomedical visualization, as well as students in those respective fields.

Download or read book Ex vivo and In vivo Optical Molecular Pathology written by Jürgen Popp and published by John Wiley & Sons. This book was released on 2014-03-14 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: The result of a unique collaboration between clinicians, chemists and physicists, this book provides an unparalleled overview of a new generation of diagnostic tools in clinical pathology. The introductory chapters cover the present status and limitations of currently used methods, followed by an outline of promising novel spectroscopy-based technologies either under development or recently available on the market. The input from both technologists developing these new methods as well as routine clinicians familiar with practical aspects and medical relevance guarantees that this practical work is a valuable asset for a wide audience, including technical personnel and decision makers in treatment centers, experts working in companies developing diagnostic devices, and clinicians specializing in advanced diagnostic methods. Since basic researchers are increasingly adopting novel diagnostic tools developed for human use as well, this will also be of interest for biomedical research institutions with large animal facilities.

Download or read book Handbook of Biomedical Image Analysis written by David Wilson and published by Springer Science & Business Media. This book was released on 2007-04-25 with total page 583 pages. Available in PDF, EPUB and Kindle. Book excerpt: Our goal is to develop automated methods for the segmentation of thr- dimensional biomedical images. Here, we describe the segmentation of c- focal microscopy images of bee brains (20 individuals) by registration to one or several atlas images. Registration is performed by a highly parallel imp- mentation of an entropy-based nonrigid registration algorithm using B-spline transformations. We present and evaluate different methods to solve the cor- spondence problem in atlas based registration. An image can be segmented by registering it to an individual atlas, an average atlas, or multiple atlases. When registering to multiple atlases, combining the individual segmentations into a ?nalsegmentationcanbeachievedbyatlasselection,ormulticlassi?erdecision fusion. Wedescribeallthesemethodsandevaluatethesegmentationaccuracies that they achieve by performing experiments with electronic phantoms as well as by comparing their outputs to a manual gold standard. The present work is focused on the mathematical and computational t- ory behind a technique for deformable image registration termed Hyperelastic Warping, and demonstration of the technique via applications in image regist- tion and strain measurement. The approach combines well-established prin- ples of nonlinear continuum mechanics with forces derived directly from thr- dimensional image data to achieve registration. The general approach does not require the de?nition of landmarks, ?ducials, or surfaces, although it can - commodate these if available. Representative problems demonstrate the robust and ?exible nature of the approach. Three-dimensional registration methods are introduced for registering MRI volumes of the pelvis and prostate. The chapter ?rst reviews the applications, xi xii Preface challenges, and previous methods of image registration in the prostate.

Download or read book Optical Fluorescence Microscopy written by Alberto Diaspro and published by Springer Science & Business Media. This book was released on 2010-11-25 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

Download or read book Superresolution Optical Microscopy written by Barry R. Masters and published by Springer Nature. This book was released on 2020-03-21 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.

Download or read book Second Harmonic Generation Imaging written by Francesco S. Pavone and published by Taylor & Francis. This book was released on 2016-04-19 with total page 465 pages. Available in PDF, EPUB and Kindle. Book excerpt: Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a

Download or read book Proceedings of Optical Biopsies and Microscopic Techniques written by Irving J. Bigio and published by SPIE-International Society for Optical Engineering. This book was released on 1996 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains 27 papers presented at the EOS/SPIE Biomedical Optics Symposium. They are divided into eight sessions, which cover topics such as fluorescence-spectroscopy diagnostics, elastic-scattering and other tissue spectroscopies; and confocal microscopy.

Download or read book Nonlinear Microscopy as a Tool for Histological Examinations written by Alexei Kazarine and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "Histological analysis is a fundamental technique in modern medical diagnostics. In this thesis, we present novel imaging techniques based on nonlinear microscopy to provide a most needed update to classical histological methods, some of which were initially developed a hundred years ago. Nonlinear microscopy, a form of advanced laser scanning microscopy, enables three-dimensional imaging within tissue using multiple complementary nonlinear contrast mechanisms including multiphoton fluorescence, second harmonic generation (SHG) and third harmonic generation (THG) each with inherent optical sectioning capabilities. The technique can be applied to both labelled and unlabeled samples, allowing for greater flexibility.This thesis first presents a new histological malaria detection method, third harmonic generation imaging scanning cytometry (THGISC). This technique is based on the combination of THG imaging, high speed motorized scanning and automated software processing. THG is a nonlinear optical process in which three incident photons interact with specific materials to combine into one photon that possesses the sum of their energies resulting in emission that is three times the incident light frequency. Hemozoin, a metabolic byproduct of the malaria parasite, has been shown to present a strong THG signal. We applied THGISC to lab-cultured parasites and malaria infected patient blood samples. We demonstrated that our technique is comparable in effectiveness to the gold standard of malaria detection by manual observation of Giemsa staining while significantly improving throughput thanks to rapid scanning and automated software-based parasitemia determination.A novel multimodal approach for virtual histology of rabbit vocal folds by label-free nonlinear microscopy and nano computed tomography is presented to investigate vocal fold regeneration after injury. Traditionally, vocal fold histology was conducted through mechanical sectioning of tissue into thin slices and conventional histological staining of the slices. Our method enables us to visualize the tissue microarchitecture, particularly extracellular matrix elements such as collagen and elastin, which are critical for the proper functioning of vocal folds. We visualize collagen through SHG and elastin via two photon fluorescence; we are also able to observe other features of interest in whole vocal folds such as muscle, chondrocytes and blood vessels through two photon fluorescence. The resulting images were used to create detailed three-dimensional models of vocal fold tissue that can be computationally cross-sectioned in software for easy visualization. Lastly, we investigate common histological dyes as THG harmonophores, enabling high resolution selective imaging of specific histological tissue features. We first verified the third order nonlinear susceptibility of hematoxylin within hematoxylin and eosin stained human skin and liver tissue sections, demonstrating highly selective nuclear staining by THG. We then identified two new THG harmonophores in common histological stains: Verhoeff-Van-Gieson and picrosirius red. We observe that the iron hematoxylin component of the Verhoeff stain acts as a selective THG harmonophore in tissue, binding to elastic fibers and nuclei. We also showed for the first time a significant nonlinear emission from picrosirius red, enabling highly specific, high contrast collagen detection by THG imaging.The application of nonlinear microscopy to histological investigations provides powerful alternatives to conventional methods. The inherent optical sectioning capability of nonlinear microscopy extends histology into the third dimension while the multimodal approach allows for simultaneous visualization of structures of interest in both unlabeled tissue and through labelling with highly selective harmonophores. We anticipate that these techniques will be adopted in the future of modern biomedical diagnostics." --

Download or read book Nonlinear Optical Microscopy for Imaging of Micro flow and Immune Response written by Yan Zeng and published by . This book was released on 2014 with total page 129 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Nonlinear Optical Microscopy of Highly Pigmented Tissues written by Ilyas Saytashev and published by . This book was released on 2016 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Four wave mixing Microscopy of Nanowires and Meibomian Glands written by Chia-Yu Lin and published by . This book was released on 2011 with total page 105 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical microscopy is a powerful tool for examining the microscopic and nanoscopic world. In many areas of science, including biology and the fabrication of new (sub)-micron structured materials, the optical microscope is an indispensable tool. Over the last two decades, the field of nonlinear optical microscopy has added new imaging capabilities that are of tremendous impact for biology and material sciences. The technique of four-wave mixing (FWM), in particular, has shown great potential as an imaging method that is able to probe material properties that have remained inaccessible using conventional techniques. Coherent anti-Stokes Raman scattering (CARS) microscopy, a FWM technique, is gained a lot of momentum as a biological imaging tool. CARS is sensitive to both the vibrational and electronic properties of materials. In this thesis, we have applied CARS microscopy to two distinct areas: biological tissue imaging and characterization of nanowires. Our study of the lipid distribution of meibomian glands highlights the vibrational sensitivity of the CARS technique. We have extend the current state-of-the-art by developing hyperspectral CARS imaging, which generates images with spectral information, and combined this with principal component analysis (PCA), a multivariate analysis technique. The results of this study provide a new look at the lipid distribution in meibomian glands, and, by linking structure to functionality, it provides clues towards the origins of dry eye disease. In a second study, we have explored the electronic sensitivity of CARS microscopy. Electronic CARS was used to interrogate the nonlinear optical properties of semiconducting nanowires. This study has revealed the optical anisotropy of polycrystalline CdSe nanowires, which offers new insights into the optical response of such nanoscopic systems. Finally, we have used our microscope system to improve the optical properties of nanowires. We have developed a laser zone annealing method for increasing the grain size of polycrystalline nanowires. Our results demonstrate that the optical and electronic properties of selected segments of the nanowire can be significantly improved.

Download or read book Coherent Light Microscopy written by Pietro Ferraro and published by Springer. This book was released on 2013-04-23 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book deals with the latest achievements in the field of optical coherent microscopy. While many other books exist on microscopy and imaging, this book provides a unique resource dedicated solely to this subject. Similarly, many books describe applications of holography, interferometry and speckle to metrology but do not focus on their use for microscopy. The coherent light microscopy reference provided here does not focus on the experimental mechanics of such techniques but instead is meant to provide a users manual to illustrate the strengths and capabilities of developing techniques. The areas of application of this technique are in biomedicine, medicine, life sciences, nanotechnology and materials sciences.