Download or read book Non canonical Amino Acids as Minimal Tags for Investigating Protein Organization and Turnover written by Ingrid-Cristiana Gebura-Vreja and published by . This book was released on 2015 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The burgeoning field of genetic code expansion provides new tools for specifically labeling proteins for a variety of applications. Clickable non-canonical amino acids (ncAA) have been refined for almost-quantitative and highly selective reactions with complementary probes. In my thesis work I have adapted genetic code expansion for two biological questions. First, I used ncAA incorporation and click reaction to test the effect of fluorescent protein (FP) tagging on the nanoscale organization of target proteins. Second, I used these tools to generate a genetically-encoded scheme for specifi ...
Download or read book Genetically Incorporated Non Canonical Amino Acids written by Yu-Hsuan Tsai and published by Springer Nature. This book was released on 2023-06-05 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.
Download or read book Non Natural Amino Acids written by and published by Academic Press. This book was released on 2009-07-24 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: By combining the tools of organic chemistry with those of physical biochemistry and cell biology, Non-Natural Amino Acids aims to provide fundamental insights into how proteins work within the context of complex biological systems of biomedical interest. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. With more than 400 volumes published, each Methods in Enzymology volume presents material that is relevant in today's labs -- truly an essential publication for researchers in all fields of life sciences. Demonstrates how the tools and principles of chemistry combined with the molecules and processes of living cells can be combined to create molecules with new properties and functions found neither in nature nor in the test tube Presents new insights into the molecular mechanisms of complex biological and chemical systems that can be gained by studying the structure and function of non-natural molecules Provides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods
Download or read book Stimulated Raman Scattering Microscopy written by Ji-Xin Cheng and published by Elsevier. This book was released on 2021-12-04 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics
Download or read book The Ubiquitin System written by Milton J. Schlesinger and published by Cold Spring Harbor Laboratory Press. This book was released on 1988 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Noncanonical Amino Acids written by Edward A. Lemke and published by . This book was released on 2018 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Memory Mechanisms in Health and Disease written by Karl Peter Giese and published by World Scientific. This book was released on 2012-06-07 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Memory Mechanisms is an edited review volume that summarizes state-of-the-art knowledge on memory mechanisms at the molecular, cellular and circuit level. Each review is written by leading experts in the field, presenting not only current knowledge, but also discussing the concepts, providing critical reflections and suggesting an outlook for future studies. The memory mechanisms are also discussed in the context of diseases. Studies of memory deficits in disease models are introduced as well as approaches to restore memory deficits. Finally, the impact of contemporary memory research for psychiatry is illustrated. Contents:Introduction (Karl Peter Giese)Long-term Potentiation and Memory (Karl Peter Giese)Structural Synaptic and Dendritic Spine Plasticity in the Hippocampus (Michael G Stewart and Victor I Popov)Memory Beyond Synaptic Plasticity: The Role of Intrinsic Neuronal Excitability (Athanasia Papoutsi, Kyriaki Sidiropoulou and Panayiota Poirazi)Adult Hippocampal Neurogenesis and Memory (Scellig S D Stone and Paul W Frankland)Memory Consolidation and Its Underlying Mechanisms (Cristina M Alberini, Dhananjay Bambah-Mukku and Dillon Y Chen)The Role of CREB and CREB Co-activators in Memory Formation (Melanie J Sekeres, Derya Sargin, P Joel Ross and Sheena A Josselyn)Sex Differences in Memory Formation (Keiko Mizuno and Karl Peter Giese)Neocortical Learning, Memory and Experience-dependent Plasticity (Giorgia Albieri and Gerald T Finnerty)Mechanisms of Memory Storage (Kimberly Lackenby and Karl Peter Giese)Memory Reconsolidation and Extinction (Satoshi Kida)Memory Circuits in Drosophila (Katsuo Furukubo-Tokunaga, Zoe N Ludlow and Frank Hirth)Memory Circuits in the Hippocampus (Thomas J McHugh)Impaired Memory Mechanisms in Mouse Models of Alzheimer's Disease (Masuo Ohno)Restoring Memory Deficits in Cognitive Diseases (Andre Fischer)The Impact of Studies of Memory Mechanisms for Psychiatry (Shahid H Zaman) Readership: Students and professionals interested in neurology/neuroscience. Keywords:Brain;Learning;Mental Health;Memory;Neuronal Networks;Plasticity;SignallingKey Features:The book provides a comprehensive overview on mechanisms of memoryThe book is useful for organizing undergraduate courses and for the teaching of medical studentsIt complements more basic text books such as “Mechanisms of Memory” by JD Sweatt, Academic Press, 2009, and it provides insights into memory dysfunction in diseases and routes for treating such deficits
Download or read book Macromolecular Protein Complexes III Structure and Function written by J. Robin Harris and published by Springer Nature. This book was released on 2020-11-30 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers important topics such as the dynamic structure and function of the 26S proteasome, the DNA replication machine: structure and dynamic function and the structural organization and protein–protein interactions in the human adenovirus capsid, to mention but a few. The 18 chapters included here, written by experts in their specific field, are at the forefront of scientific knowledge. The impressive integration of structural data from X-ray crystallography with that from cryo-electron microscopy is apparent throughout the book. In addition, functional aspects are also given a high priority. Chapter 1 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Download or read book Engineering the Genetic Code written by Nediljko Budisa and published by John Wiley & Sons. This book was released on 2006-05-12 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to introduce non-canonical amino acids in vivo has greatly expanded the repertoire of accessible proteins for basic research and biotechnological application. Here, the different methods and strategies to incorporate new or modified amino acids are explained in detail, including a lot of practical advice for first-time users of this powerful technique. Novel applications in protein biochemistry, genomics, biotechnology and biomedicine made possible by the expansion of the genetic code are discussed and numerous examples are given. Essential reading for all molecular life scientists who want to stay ahead in their research.
Download or read book Plant Systems Biology written by Sacha Baginsky and published by Springer Science & Business Media. This book was released on 2007-06-25 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume aims to provide a timely view of the state-of-the-art in systems biology. The editors take the opportunity to define systems biology as they and the contributing authors see it, and this will lay the groundwork for future studies. The volume is well-suited to both students and researchers interested in the methods of systems biology. Although the focus is on plant systems biology, the proposed material could be suitably applied to any organism.
Download or read book Prokaryotic Metabolism and Physiology written by Byung Hong Kim and published by Cambridge University Press. This book was released on 2019-05-16 with total page 509 pages. Available in PDF, EPUB and Kindle. Book excerpt: Extensive and up-to-date review of key metabolic processes in bacteria and archaea and how metabolism is regulated under various conditions.
Download or read book Physical Biology of the Cell written by Rob Phillips and published by Garland Science. This book was released on 2012-10-29 with total page 1089 pages. Available in PDF, EPUB and Kindle. Book excerpt: Physical Biology of the Cell is a textbook for a first course in physical biology or biophysics for undergraduate or graduate students. It maps the huge and complex landscape of cell and molecular biology from the distinct perspective of physical biology. As a key organizing principle, the proximity of topics is based on the physical concepts that
Download or read book The Condensed Protocols from Molecular Cloning written by Joseph Sambrook and published by CSHL Press. This book was released on 2006 with total page 802 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Condensed Protocols From Molecular Cloning: A Laboratory Manualis a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual.This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world's best–selling manual of molecular biology techniques. Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning.
Download or read book TRNA written by Dieter Söll and published by . This book was released on 1995 with total page 572 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Extracellular Matrix an Overview written by Robert Mecham and published by Springer Science & Business Media. This book was released on 2011-02-16 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knowledge of the extracellular matrix (ECM) is essential to understand cellular differentiation, tissue development, and tissue remodeling. This volume of the series “Biology of Extracellular Matrix” provides a timely overview of the structure, regulation, and function of the major macromolecules that make up the extracellular matrix. It covers topics such as collagen types and assembly of collagen-containing suprastructures, basement membrane, fibronectin and other cell-adhesive glycoproteins, proteoglycans, microfibrils, elastin, fibulins and matricellular proteins, such as thrombospondin. It also explores the concept that ECM components together with their cell surface receptors can be viewed as intricate nano-devices that allow cells to physically organize their 3-D-environment. Further, the role of the ECM in human disease and pathogenesis is discussed as well as the use of model organisms in elucidating ECM function.
Download or read book Enzymatic Bioelectrocatalysis written by Kenji Kano and published by Springer Nature. This book was released on 2020-11-13 with total page 135 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the fundamental aspects of the electrochemistry and redox enzymes that underlie enzymatic bioelectrocatalysis, in which a redox enzyme reaction is coupled with an electrode reaction. Described here are the basic concept and theoretical aspects of bioelectrocatalysis and the various experimental techniques and materials used to study and characterize related problems. Also included are the various applications of bioelectrocatalysis to bioelectrochemical devices including biosensors, biofuel cells, and bioreactors. This book is a unique source of information in the area of enzymatic bioelectrocatalysis, approaching the subject from a cross-disciplinary point of view.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2003 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt:
