Download or read book New Approaches in Correlative Studies of Biological Ultrastructure by High resolution Electron Microscopy written by H. Fernandez-Moran and published by . This book was released on 1964 with total page 48 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Correlative Light and Electron Microscopy IV written by and published by Academic Press. This book was released on 2021-03-09 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: Correlative Light and Electron Microscopy IV, Volume 162, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Besides the detailed description of protocols for CLEM technologies including time-resolution, Super resolution LM and Volume EM, new chapters cover Workflow (dis)-advantages/spiderweb, Serial section LM + EM, Platinum clusters as CLEM probes, Correlative Light Electron Microscopy with a transition metal complex as a single probe, SEM-TEM-SIMS, HPF-CLEM, A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes, and more. Contains contributions from experts in the field Covers topics using nano-SIMS and EDX for CLEM Presents recent advances and currently applied correlative approaches Gives detailed protocols, allowing for the application of workflows in one’s own laboratory setting Covers CLEM approaches in the context of specific applications Aims to stimulate the use of new combinations of imaging modalities
Download or read book Correlative Light and Electron Microscopy II written by and published by Academic Press. This book was released on 2014-09-30 with total page 493 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Cell Biology looks at methods for analyzing correlative light and electron microscopy (CLEM). With CLEM, people try to combine the advantages of both worlds, i.e. the dynamics information obtained by light microscopy and the ultrastructure as provided by electron microscopy. This volume contains the latest techniques on correlative microscopy showing that combining two imaging modalities provides more than each technique alone. Most importantly it includes the essential protocols, including tips, tricks and images for you to repeat these exciting techniques in your own lab. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material Second of two volumes dedicated to Correlative Light and Electron microscopy (CLEM)
Download or read book Correlative Light and Electron Microscopy III written by and published by Academic Press. This book was released on 2017-05-23 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: Correlative Light and Electron Microscopy III, Volume 140, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics discussed in this new release include Millisecond time-resolved CLEM, Super resolution LM und SEM of high-pressure frozen C. elegans, Preservation fluorescence, super res CLEM, APEX in Tissue, Corrsight mit IBIDI flowthrough chamber, Correlative Light Atomic Force Electronic Microscopy (CLAFEM), Atmospheric EM CLEM, and High-precision correlation, amongst other topics. Chapters in this ongoing series deal with different approaches for analyzing the same specimen using more than one imaging technique. The strengths and application area of each is presented, with this volume exploring the aspects of sample preparation of diverse biological systems for different CLEM approaches. Contains contributions from experts in the field Covered topics include targeted ultramicrotomy and high-precision correlation Presents recent advances and currently applied correlative approaches Gives detailed protocols allowing the application of workflows in one’s own laboratory setting Covers CLEM approaches in the context of specific applications Aims to stimulate the use of new combinations of imaging modalities
Download or read book Correlative Light and Electron MIcroscopy written by Thomas Muller-Reichert and published by Academic Press. This book was released on 2012-08-10 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods
Download or read book Three Dimensional Electron Microscopy written by and published by Academic Press. This book was released on 2019-07-18 with total page 303 pages. Available in PDF, EPUB and Kindle. Book excerpt: Three-Dimensional Electron Microscopy, Volume 152 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters focusing on FIB-SEM of mouse nervous tissue: fast and slow sample preparation, Serial-section electron microscopy using ATUM - Automated Tape collecting Ultra-Microtome, Software for automated acquisition of electron tomography tilt series, Scanning electron tomography of biological samples embedded in plastic, Cryo-STEM tomography for Biology, CryoCARE: Content-aware denoising of cryo-EM images and tomograms using artificial neural networks, Expedited large-volume 3-D SEM workflows for comparative vertebrate microanatomical imaging, and many other interesting topics. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Includes the latest information on the Three-Dimensional Electron Microscopy technique
Download or read book Correlative Light and Electron Microscopy V written by Thomas Müller-Reichert and published by Elsevier. This book was released on 2024-05-04 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Correlative Light and Electron Microscopy V, Volume 187 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Includes the latest information on the topic of development, characterization and applications in CAR T Cells
Download or read book Volume Electron Microscopy written by and published by Elsevier. This book was released on 2023-07-13 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume Electron Microscopy (vEM), Volume 177 is a collective term for a set of three-dimensional high-resolution ultrastructural imaging techniques that have delivered new insights into biological systems in recent years, garnering substantial interest in the life and clinical sciences. In this book, users will find a variety of vEM workflows and technologies, highlighting application areas with biologically relevant examples. Topics covered include Automated large volume sample preparation for vEM, Resin comparison for Serial Block Face Scanning Volume Electron Microscopy, Immunolabelling for SBF-SEM, Electron Microscopy in Plants, Serial section electron tomography, Automated Tape Collecting Ultramicrotomy (ATUM) for targeting neuropathology, Array tomography, and much more. Other sections focus on Mitochondria morphometry in 3d datasets of mouse brain obtained with serial block-face Scanning Electron Microscopy, Serial Block-face Scanning Electron Microscopy of Schmidtea mediterranea, Correlative multiscale microCT-SBF-SEM imaging of resin-embedded tissue, Methods of enhanced FIB-SEM sample preparation and image acquisition, Functional characterization of endo-lysosomal compartments by correlative live-cell and volume electron microscopy, and much more. Includes chapters written by key leaders and developers in the field Provides detailed protocols, allowing for the application of workflows in one’s own laboratory setting Presents real tips and tricks you won't get from standard research papers
Download or read book Development and Applications of New Methods for High Resolution Transmission Electron Microscopy of Biological Macromolecules written by Craig Allen Smith and published by . This book was released on 1979 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Interpretation of Ultrastructure written by R. J. C. Harris and published by Academic Press. This book was released on 2014-07-15 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Interpretation of Ulstrastructure is a collection of papers dealing with techniques in ulstrastructural research such as in fixation, reversed staining, ultrastructure in embryos and in plants, control of observations by light microscopy, and cytochemistry at the electron microscope level. One paper describes various fixatives used on rat liver tissue including a standard osmium tetroxide solution, pure melted osmium tetroxide, and strong osmium tetroxide solutions in carbon tetrachloride. Another paper points out that the use of electron micrographs of the cell nucleus and chromosomes should include approaches to chromosome analysis. For the investigator studying the three dimensional organization of chromosomes, his approaches should include classical cytology and cytogenetics, chemical and cytochemical studies on nuclei, as well as chemical and physical analysis of nucleic acid and nucleoprotein molecules. Several papers discuss the study of natural and artificial DNA-plasma by thin sections, an assessment of negative staining techniques for revealing ultrastructure, and other approaches in the study of biological ultrastructure by high-resolution electron microscopy. The collection will benefit microbiologists, biotechnologists, and academicians connected with the biological sciences.
Download or read book Cellular Imaging written by Eric Hanssen and published by Springer. This book was released on 2017-12-30 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation
Download or read book Scientific and Technical Aerospace Reports written by and published by . This book was released on 1964 with total page 1592 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Ultrastructural Studies by Correlative Stochastic Optical Reconstruction Microscopy and Electron Microscopy written by Doory Kim and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence light microscopy (LM) and electron microscopy (EM) are two of the most widely used imaging modalities for probing cellular structures. In this dissertation I present our works in both developing methods of several correlative super-resolution fluorescence light microscopy (LM) and electron microscopy (EM) assays by combining stochastic optical reconstruction microscopy (STORM), a super-resolution imaging technique with several different EM imaging modalities and applying super-resolution microscopy to investigate the distributions and interactions of purine biosynthetic enzymes organization complex called purinosomes within the cell.
Download or read book Correlative Imaging written by Paul Verkade and published by John Wiley & Sons. This book was released on 2019-11-04 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: Brings a fresh point of view to the current state of correlative imaging and the future of the field This book provides contributions from international experts on correlative imaging, describing their vision of future developments in the field based on where it is today. Starting with a brief historical overview of how the field evolved, it presents the latest developments in microscopy that facilitate the correlative workflow. It also discusses the need for an ideal correlative probe, applications in proteomic and elemental analysis, interpretation methods, and how correlative imaging can incorporate force microscopy, soft x-ray tomography, and volume electron microscopy techniques. Work on placing individual molecules within cells is also featured. Correlative Imaging: Focusing on the Future offers in-depth chapters on: correlative imaging from an LM perspective; the importance of sample processing for correlative imaging; correlative light and volume EM; correlation with scanning probe microscopies; and integrated microscopy. It looks at: cryo-correlative microscopy; correlative cryo soft X-ray imaging; and array tomography. Hydrated-state correlative imaging in vacuo, correlating data from different imaging modalities, and big data in correlative imaging are also considered. Brings a fresh view to one of the hottest topics within the imaging community: the correlative imaging field Discusses current research and offers expert thoughts on the field’s future developments Presented by internationally-recognized editors and contributors with extensive experience in research and applications Of interest to scientists working in the fields of imaging, structural biology, cell biology, developmental biology, neurobiology, cancer biology, infection and immunity, biomaterials and biomedicine Part of the Wiley–Royal Microscopical Society series Correlative Imaging: Focusing on the Future will appeal to those working in the expanding field of the biosciences, correlative microscopy and related microscopic areas. It will also benefit graduate students working in microscopy, as well as anyone working in the microscopy imaging field in biomedical research.
Download or read book NASA Scientific and Technical Reports written by United States. National Aeronautics and Space Administration Scientific and Technical Information Division and published by . This book was released on 1965 with total page 1152 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Proceedings written by and published by . This book was released on 1967 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electron Microscopy Of Subcellular Dynamics written by Helmut Plattner and published by CRC Press. This book was released on 1989-09-30 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: This illustrated volume surveys the correlated use of currently available methods of electron microscopic techniques, along with the goals and perspectives for future developments. The authors discuss an integrative approach of different EM preparation and analysis techniques that can allow for an analysis of dynamic cellular processes with high temporal and spatial resolution on the electron microscope level. This concise, yet thorough, work is a valuable reference for researchers in the field.
