Download or read book Multi scale 3D Virtual Histology Via Phase contrast X ray Tomography with Synchrotron Radiation written by Jasper Frohn and published by Universitätsverlag Göttingen. This book was released on 2023 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: To this day, the standard method for investigating biological tissue with cellular resolution is the examination under a light microscope, first denoted as histology by Karl Meyer in 1819. Despite the enormous success and importance of histology, it has two major disadvantages. Firstly, the specimen must be physically cut into thin sections due to the limited penetrating power of optical light, and secondly, additional staining of the specimen is required to achieve sufficient image contrast. Both disadvantages can be overcome by the non-destructive method of propagation-based X-ray phase-contrast tomography. While the mechanism of phase-contrast provides sufficient image contrast to image single cells, a tomographic imaging scheme with penetrating X-rays allows for an undamaged sample by virtually slicing the reconstructed 3D sample volume. In this work, the holotomography setup of the synchrotron endstation „GINIX“ (The Göttingen Instrument for Nanoscale-Imaging with X-Rays) was extended to a multi-scale X-ray phase-contrast tomography setup suitable for 3D virtual histology by adding two acquisition schemes. Compared to the existing setup, the first additional scheme is a propagation-based microtomography setup, which enlarges the reconstructed 3D volumes by a factor of approx. 64 at a fraction of the acquisition time (ca. 2 min). The second additional scheme aims for higher resolutions. To this end, the X-ray waveguide illumination was combined with photon counting detector with a large field of view and a novel phase reconstruction scheme, which is based on iterative farfield phase retrieval without an „empty-beam correction“ in the detector plane.
Download or read book Multi scale 3D Virtual Histology Via Phase contrast X ray Tomography with Synchrotron Radiation written by Jasper Frohn and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Multiscale X ray Structural Analysis of Cardiac Cells and Tissues written by Marius Reichardt and published by Universitätsverlag Göttingen. This book was released on 2022 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: The cardiac function relies on an intricate molecular and cellular three-dimensional (3d) architecture of a complex, dense and co-dependent cellular network. Structural alterations of the cardiac structure can affect its essential function and lead to severe dysfunction of the organ. Cardiovascular diseases are the main cause of death worldwide with a rising incidence. However, it is not possible to give a generalized answer how the heart is formed. Up to now, cardiac structure as well as physiologic and disease-related tissue alterations of the tissue are mainly investigated by established 2d imaging methods such as optical microscopy or electron microscopy. This work presents a multiscale and multimodal X-ray imaging approach, which allows to probe the heart structure from the scale of entire intact murine hearts to the molecular organisation of the sarcomer structure. While the molecular structure of the actomyosin complex is probed by scanning X-ray diffraction, the 3d arrangement of the cellular network is investigated by propagation-based X-ray phase-contrast tomography. In this context, the concept of 3d virtual histology of cardiac tissue by X-ray phase-contrast tomography using laboratory sources as well as highly coherent synchrotron radiation is being further developed.
Download or read book Novel approaches in cardiac imaging written by Giuseppe Pannarale and published by Frontiers Media SA. This book was released on 2023-06-30 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Advancing the Characterization of Neuronal Cyto Architecture by X ray Phase Contrast Tomography written by Marina Eckermann and published by Universitätsverlag Göttingen. This book was released on 2021 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: To bring physiology and pathology of the human brain into better micro-anatomical and histological context, studies with different methodologies are required. Established techniques such as electron microscopy or histology show limitations in view of invasiveness, labor-intense and artifact-prone sample preparation, as well as an adequate ratio between resolution and volume throughput. For this reason, X-ray phase-contrast tomography (PC-CT) has been proposed as a three-dimensional non-destructive imaging technique, which requires less effort in sample preparation and can assess larger volumes. Furthermore, it offers quantitative electron density based contrast even for unstained tissue. Up to now, however, PC-CT studies fell short in number of samples, so that structural alterations caused by neurodegenerative diseases cannot be distinguished from physiological inter-subject variations. In this thesis, the scalability of PC-CT with respect to the required number of samples and resolution-to-volume-throughput is demonstrated, and the methodology is advanced with respect to data acquisition, processing and segmentation. In addition to the human cerebellum, cortex and hippocampus are studied. Concerning quantification and analysis of PC-CT data, this work introduces optimal transport analysis to obtain quantitative metrics of the cyto-architecture and to identify changes due to neurodegenerative diseases. For the case of Alzheimer’s disease, this workflow reveals a yet undescribed compactification of granular cells in the human hippocampus. This thesis also provides optimized configurations to study neural tissues with laboratory instrumentation, and – finally – provides new correlative imaging approaches, in particular with scanning electron microscopy.
Download or read book Vertebrate Skeletal Histology and Paleohistology written by Vivian de Buffrénil and published by CRC Press. This book was released on 2021-06-24 with total page 838 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vertebrate Skeletal Histology and Paleohistology summarizes decades of research into the biology and biological meaning of hard tissues, in both living and extinct vertebrates. In addition to outlining anatomical diversity, it provides fundamental phylogenetic and evolutionary contexts for interpretation. An international team of leading authorities review the impact of ontogeny, mechanics, and environment in relation to bone and dental tissues. Synthesizing current advances in the biological problems of growth, metabolism, evolution, ecology, and behavior, this comprehensive and authoritative volume is built upon a foundation of concepts and technology generated over the past fifty years.
Download or read book Nanoscale Photonic Imaging written by Tim Salditt and published by Springer Nature. This book was released on 2020-06-09 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book, edited and authored by a team of world-leading researchers, provides a broad overview of advanced photonic methods for nanoscale visualization, as well as describing a range of fascinating in-depth studies. Introductory chapters cover the most relevant physics and basic methods that young researchers need to master in order to work effectively in the field of nanoscale photonic imaging, from physical first principles, to instrumentation, to mathematical foundations of imaging and data analysis. Subsequent chapters demonstrate how these cutting edge methods are applied to a variety of systems, including complex fluids and biomolecular systems, for visualizing their structure and dynamics, in space and on timescales extending over many orders of magnitude down to the femtosecond range. Progress in nanoscale photonic imaging in Göttingen has been the sum total of more than a decade of work by a wide range of scientists and mathematicians across disciplines, working together in a vibrant collaboration of a kind rarely matched. This volume presents the highlights of their research achievements and serves as a record of the unique and remarkable constellation of contributors, as well as looking ahead at the future prospects in this field. It will serve not only as a useful reference for experienced researchers but also as a valuable point of entry for newcomers.
Download or read book Computed Tomography written by Willi A. Kalender and published by John Wiley & Sons. This book was released on 2011-07-07 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book offers a comprehensive and user-oriented description of the theoretical and technical system fundamentals of computed tomography (CT) for a wide readership, from conventional single-slice acquisitions to volume acquisition with multi-slice and cone-beam spiral CT. It covers in detail all characteristic parameters relevant for image quality and all performance features significant for clinical application. Readers will thus be informed how to use a CT system to an optimum depending on the different diagnostic requirements. This includes a detailed discussion about the dose required and about dose measurements as well as how to reduce dose in CT. All considerations pay special attention to spiral CT and to new developments towards advanced multi-slice and cone-beam CT. For the third edition most of the contents have been updated and latest topics like dual source CT, dual energy CT, flat detector CT and interventional CT have been added. The enclosed CD-ROM again offers copies of all figures in the book and attractive case studies, including many examples from the most recent 64-slice acquisitions, and interactive exercises for image viewing and manipulation. This book is intended for all those who work daily, regularly or even only occasionally with CT: physicians, radiographers, engineers, technicians and physicists. A glossary describes all the important technical terms in alphabetical order. The enclosed DVD again offers attractive case studies, including many examples from the most recent 64-slice acquisitions, and interactive exercises for image viewing and manipulation. This book is intended for all those who work daily, regularly or even only occasionally with CT: physicians, radiographers, engineers, technicians and physicists. A glossary describes all the important technical terms in alphabetical order.
Download or read book 3d virtual histology of neuronal tissue by propagation based x ray phase contrast tomography written by Mareike Töpperwien and published by Göttingen University Press. This book was released on 2018 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: Deciphering the three-dimensional (3d) cytoarchitecture of neuronal tissue is an important step towards understanding the connection between tissue function and structure and determining relevant changes in neurodegenerative diseases. The gold standard in pathology is histology, in which the tissue is examined under a light microscope after serial sectioning and subsequent staining. It is an invasive and labor-intensive technique which is prone to artifacts due to the slicing procedure. While it provides excellent results on the 2d slices, the 3d anatomy can only be determined after aligning the individual sections, leading to a non-isotropic resolution within the tissue. X-ray computed tomography (CT) offers a promising alternative due to its potential resolution and large penetration depth which allows for non-invasive imaging of the sample's 3d density distribution. In classical CT, contrast formation is based on absorption of the x-rays as they pass through the sample. However, weakly absorbing samples like soft tissue from the central nervous system give nearly no contrast. By exploiting the much stronger phase shifts for contrast formation, which the sample induces in a (partially) coherent wavefront, it can be substantially increased. During free-space propagation behind the sample, these phase shifts are converted to a measurable intensity image by interference of the disturbed wave fronts. In this thesis, 3d virtual histology is performed by means of propagation-based x-ray phase-contrast tomography on tissue from the central nervous system of humans and mice. A combination of synchrotron-based and laboratory setups is used to visualize the 3d density distribution on varying lengths scales from the whole organ down to single cells. By comparing and optimizing different preparation techniques and phase-retrieval approaches, even sub-cellular resolution can be reached in mm-sized tissue blocks. The development of an automatic cell segmentation workflow provides access to the 3d cellular distribution within the tissue, enabling the quantification of the cellular arrangement and allowing for extensive statistical analysis based on several thousands to millions of cells. This paves the way for biomedical studies aimed at changes in cellular distribution, e.g., in the course of neurodegenerative diseases such as multiple sclerosis, Alzheimer's disease or ischemic stroke.
Download or read book MicroComputed Tomography written by Stuart R. Stock and published by CRC Press. This book was released on 2008-12-23 with total page 366 pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to the availability of commercial laboratory systems and the emergence of user facilities at synchrotron radiation sources, studies of microcomputed tomography or microCT have increased exponentially. MicroComputed Technology provides a complete introduction to the technology, describing how to use it effectively and understand its results. The first part of the book focuses on methodology, covering experimental methods, data analysis, and visualization approaches. The second part addresses various microCT applications, including porous solids, microstructural evolution, soft tissue studies, multimode studies, and indirect analyses. The author presents a sufficient amount of fundamental material so that those new to the field can develop a relative understanding of how to design their own microCT studies. One of the first full-length references dedicated to microCT, this book provides an accessible introduction to field, supplemented with application examples and color images.
Download or read book Fish as Model Organism for Skeletal Diseases written by Erika Kague and published by Frontiers Media SA. This book was released on 2023-11-30 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book X ray Phase Contrast Imaging Using Near Field Speckles written by Marie-Christine Zdora and published by Springer Nature. This book was released on 2021-02-16 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis presents research on novel X-ray imaging methods that improve the study of specimens with small density differences, revealing their inner structure and density distribution. Exploiting the phase shift of X-rays in a material can significantly increase the image contrast compared to conventional absorption imaging. This thesis provides a practical guide to X-ray phase-contrast imaging with a strong focus on X-ray speckle-based imaging, the most recently developed phase-sensitive method. X-ray speckle-based imaging only requires a piece of abrasive paper in addition to the standard X-ray imaging setup. Its simplicity and robustness combined with the compatibility with laboratory X-ray sources, make it an ideal candidate for wide user uptake in a range of fields. An in-depth overview of the state of the art of X-ray speckle-based imaging and its latest developments is given in this thesis. It, furthermore, explores a broad range of applications, from X-ray optics characterisation, to biomedical imaging for 3D virtual histology and geological studies of volcanic rocks, demonstrating is promising potential. Moreover, the speckle-based technique is placed in the context of other phase-sensitive X-ray imaging methods to assist in the choice of a suitable method, hence serving as a guide and reference work for future users.
Download or read book Coherent X Ray Optics written by David Paganin and published by Oxford University Press on Demand. This book was released on 2006-01-12 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: X-ray optics is undergoing a renaissance, which may be paralleled to that experienced by visible-light optics following the invention of the laser. The associated surge of activity in "coherent" x-ray optics has been documented in this monograph, the first of its type in the field.
Download or read book Root Engineering written by Asunción Morte and published by Springer Science & Business Media. This book was released on 2014-04-12 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume illustrates the complex root system, including the various essential roles of roots as well as their interaction with diverse microorganisms localized in or near the root system. Following initial chapters describing the anatomy and architecture as well as the growth and development of root systems, subsequent chapters focus on the various types of root symbiosis with bacteria and fungi in the rhizosphere. A third section covers the physiological strategies of roots, such as nitrate assimilation, aquaporins, the role of roots in plant defense responses and in response to droughts and salinity changes. The book’s final chapters discuss the prospects of applied engineering of roots, i.e., inventing new root structures or functions through genetic modification, but also with conventional breeding and manipulation of root symbionts. The budding field of root engineering is expected to promote a second green revolution.
Download or read book X ray waveguide optics written by Sarah Hoffmann-Urlaub and published by Göttingen University Press. This book was released on 2017 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern x-ray sources and analysis techniques such as lens less imaging combined with phase retrieval algorithms allow for resolving structure sizes in the nanometer range. For this purpose optics have to be employed, ensuring small focal spot dimensions simultaneously with high photon densities. Furthermore, the wave front behind the optics is required to be smooth enabling for high resolution imaging. Combining all these properties, x-ray waveguides are well suited to perform this task, since the intensity distribution behind the guide is restricted in two dimensions serving as a secondary quasi point-source without wave-front aberrations, showing also a high divergence, suitable for resolving fine features. Importantly, the radiation provided by the waveguide reveals a high degree of coherence, required by many imaging techniques. The waveguide itself consists of an air-filled channel embedded in a solid matrix; typical materials are silicon, germanium or quartz. While the entrance area is nano-sized, the channel length is in the millimeter-range, this way posing challenges to fabricate high aspect ratio geometries. Since the functioning of x-ray waveguides is based on the total reflection at small incident angles, the surface roughness of the channel walls must be as low as possible to avoid scattering and hence loss of intensity. To fulfill these demanding conditions, a process scheme involving spin-coating, electron beam lithography, wet development, reactive ion etching and wafer bonding is optimized within this work. To gain deeper insights into the principle of wave guiding finite difference simulations are performed, also opening access for advanced design considerations such as gratings, tapered and curved channels, or beamsplitters, enabling for constructing novel x-ray tools as for example time delay devices or interferometers. Waveguides in all geometries are tested at synchrotron sources, accomplishing new benchmarks in x-ray optical performance. Here, the x-ray beam leaving the channel, propagates out to a pixel array detector in the far-field region. From the recorded data the intensity distribution in the near-field directly behind the waveguide is reconstructed, revealing an outstanding agreement with the simulations and electron micrographs. Since the radiation field of the waveguide is well-characterized and also tunable to meet the requirements of both the measurement setup and the sample, they are suited of a broad field of applications in coherent x-ray imaging.
Download or read book Medical Imaging Systems written by Andreas Maier and published by Springer. This book was released on 2018-08-02 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book gives a complete and comprehensive introduction to the fields of medical imaging systems, as designed for a broad range of applications. The authors of the book first explain the foundations of system theory and image processing, before highlighting several modalities in a dedicated chapter. The initial focus is on modalities that are closely related to traditional camera systems such as endoscopy and microscopy. This is followed by more complex image formation processes: magnetic resonance imaging, X-ray projection imaging, computed tomography, X-ray phase-contrast imaging, nuclear imaging, ultrasound, and optical coherence tomography.
Download or read book Brilliant Light in Life and Material Sciences written by Vasili Tsakanov and published by Springer Science & Business Media. This book was released on 2007-09-18 with total page 483 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains an excellent overview of the status and highlights of brilliant light facilities and their applications in biology, chemistry, medicine, materials and environmental sciences. Overview papers on diverse fields of research by leading experts are accompanied by the highlights in the near and long-term perspectives of brilliant X-Ray photon beam usage for fundamental and applied research.
