Download or read book Mouse Models of Allergic Inflammation written by Lara Utsch Mendes Gouveia and published by . This book was released on 2017 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Allergic diseases are the result of an aberrant immune response by T helper type 2 cells to allergens. Allergic sensitization is considered to be a multifactorial process that involves among others, environmental factors, genetics and characteristics of allergens. In normal conditions, exposure to non-infectious protein antigens induces a state of tolerance, which is essential to maintain homeostasis under constant exposure to environmental antigens. A failure in the mechanisms of tolerance, leads to activation of pro-inflammatory pathways and the development of allergen specific T cell responses. The development of animal models of various allergies has been beneficial in allowing extensive investigations into mechanisms involved in the allergic pathways. These animal models can be used to predict potential risk factors as well as to test novel treatments and immunotherapy. The risk of anaphylaxis in humans is a limiting factor for the development of allergen-based immunotherapy. In this context, animal models can play an essential role in providing a platform for refining therapeutic treatments and ensuring safety, prior to application in humans. In this thesis we use animal models to address essential questions such as what makes an individual respond to allergens. We have studied this question from different perspectives and have identified a role for the capacity to cope with allergen induced oxidative stress; the role of epithelial contact with HDM allergens in the development of an IgE response; the immunological interplay between allergic immune responses directed against unrelated allergens in different compartments of the body and; the immunological interplay between cross-reactive allergens."--Samenvatting auteur.
Download or read book Mouse Models for Allergic Asthma written by and published by . This book was released on 2006 with total page 92 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Immune Regulation in Mouse Models of Allergic Asthma written by Yung-Chang Su and published by . This book was released on 2006 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mouse Models of Allergic Disease written by Irving C. Allen and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last half century, a dramatic increase in allergic diseases has been observed throughout industrialized nations, which has resulted in significant worldwide socio-economic challenges. In Mouse Models of Allergic Disease: Methods and Protocols, a wide range of expert contributors provide detailed protocols for the design and execution of experiments to thoroughly analyze critical elements associated with a diverse range of allergic diseases, all through the lens of mouse models that accurately recapitulate clinically relevant aspects of the respective human disease. The volume opens with a section featuring techniques essential for effective ex vivo cell isolation and evaluation of specific cell types relevant to a diverse range of allergic diseases, and the book then moves on to cover in vivo protocols to evaluate prevalent mouse models of human allergic diseases, including mouse models of systemic anaphylaxis, contact hypersensitivity, allergic rhinitis, and asthma, as well as a collection of chapters on in vivo and ex vivo protocols used to assess indirect mediators of allergic diseases, such as the nervous system, non-hematopoietic cells, and the composition of the gut microbiome. Written in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Timely and authoritative, Mouse Models of Allergic Disease: Methods and Protocols serves as an essential collection of protocols that allow both novice and expert researchers the ability to accurately develop, evaluate, and characterize the mechanisms associated with these disorders.
Download or read book Animal Models of Allergic Disease written by Kumi Nagamoto-Combs and published by Humana. This book was released on 2021-12-08 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides protocols for mouse models of allergic diseases and guidelines for choosing a particular strains, allergen, adjuvant, and route of sensitization. Chapters detail types of allergic disease, methods that are frequently employed to analyze pathophysiology of allergic diseases, manipulation of intestinal microbiota, and desensitization of immure responses in animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Animal Models of Allergic Disease: Methods and Protocols aims to offer a comprehensive collection of protocols and experience-derived instructions to further allergic disease research.
Download or read book Pre Clinical Models written by Paul C. Guest and published by Humana Press. This book was released on 2019-02-04 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details reviews and protocols on the development and analysis of both cellular and animal-based pre-clinical models in a number of medical areas, including metabolic disorders, longevity, cancer, heart disease and psychiatric disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Pre-Clinical Models: Techniques and Protocols aims to provide methods that describe the context of specific disease or therapeutic areas.
Download or read book The Effects of PM2 5 on Allergic Inflammation in Mast Cell Deficient Mice written by and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal models of asthma have confirmed epidemiological findings that exposure to fine particulate matter (PM2.5) can enhance asthmatic symptoms, including eosinophilic inflammation and airway hyperresponsiveness. Critics have dismissed the possibility that these studies utilizing artificial exposure scenarios, like intratracheal instillation (i.t.), can be legitimately extrapolated to human risk largely due to the fact that the doses required for this type of model exceed the normal ambient concentrations of PM2.5. In order to improve the credibility of the findings from previous animal studies utilizing the i.t. method for delivery of aqueous particle suspensions to the lung, and to determine the biological mechanisms responsible for the observed enhancement of allergic inflammation following PM2.5 exposure, large-scale air samplers have been developed making it possible to directly expose wild type (WT) and genetically altered mice to fine, concentrated ambient particles (CAPs). In this study allergic asthma was modeled in both WT and mast cell deficient (MCD) mice by local (L) or systemic (S) sensitization to ovalbumin (OVA). Two weeks later mice were challenged with OVA (day 0) and then exposed to CAPs (day 0 & 1) with numerous endpoints collected (day 0-2). Overall, there was a temporal difference in the bronchoalveolar lavage cell profile between L and S sensitized mice, and the contribution of mast cells (MC) to this differential response was best observed for neutrophils at day 0 and day 1. Compared to air exposed mice, CAPs depressed total inflammatory cell infiltrates in the bronchoalveolar lavage fluid at day 0 and day 1 after OVA challenge for all groups. This overwhelming difference of limited cellular infiltration of monocytes and neutrophils in the bronchoalveolar lavage fluid following CAPs exposure, and the significant difference between the L and S sensitization protocols, confound interpretation for all of the factors examined. However, the specif.
Download or read book Mechanisms of Adenosine mediated Bronchoconstriction and Inflammation in an Allergic Mouse Model of Asthma written by Peter J. Oldenburg and published by . This book was released on 2005 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Murine Models of Lung Inflammation written by Vivek Chander Mathrani and published by . This book was released on 2006 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Immunoproteasome Inhibition Reduces the T Helper 2 Response in Mouse Models of Allergic Airway Inflammation written by Franziska Oliveri and published by . This book was released on 2022 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book In Vivo Models of Inflammation written by Lisa A. Marshall and published by Birkhäuser. This book was released on 2013-11-11 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this volume in the series Progress in Inflammation Research is to pro vide the biomedical researcher with a description of the state of the art of the devel opment and use of animal models of diseases with components of inflammation. Par ticularly highlighted are those models which can serve as in vivo correlates of diseases most commonly targeted for therapeutic intervention. The format is designed with the laboratory in mind; thus it provides detailed descriptions of the methodologies and uses of the most significant models. Also, new approaches to the development of future models in selected therapeutic areas have been highlighted. While emphasis is on the newest models, new information broadening our understanding of several well-known models of proven clinical utility is included. In addition, we have pro vided coverage of transgenic and gene transfer technologies which will undoubtedly serve as tools for many future approaches. Provocative comments on the cutting edge and future directions are meant to stimulate new thinking. Of course, it is important to recognize that the experimental use of animals for human benefit carries with it a solemn responsibility for the welfare of these animals. The reader is referred to the section on current regulations governing animal use which addresses this concern. To fulfill our purpose, the content is organized according to therapeutic areas with the associated models arranged in subcategories of each therapeutic area.
Download or read book Prevention of Airway and Intestinal Inflammation in a Humanized Mouse Model of Allergy by Depletion of Natural Killer Cells Prior to PBMC Engraftment written by Joachim Saloga and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Recently, we have developed a humanized mouse model of allergen-induced IgE-dependent gut and lung inflammation in PBMC-engrafted immunodeficient mice. As natural killer (NK) cells have been shown to promote allergen sensitization, type-2 immune responses and airway hyperreactivity, the aim of the present study was to investigate the impact of NK cells in this model. Therefore, NOD-scid gamma chain knock-out mice were injected intraperitoneally with human PBMC or NK cell-depleted PBMC from highly sensitized birch or grass pollen allergic donors together with the respective allergen or with NaCl as control. After an additional allergen boost one week later, mice were challenged with the allergen rectally on day 21 and gut inflammation was monitored by video mini-endoscopy evaluating translucency, granularity, fibrin production, vascularity, and stool. Then, mice were further challenged intranasally on two subsequent days and airway inflammation was measured by invasive body plethysmography and by histology. Allergen-specific human IgE in mouse sera, if detectable after co-injection of the respective allergen, was reduced in mice being injected with NK cell-depleted PBMC compared to mice which received non-depleted PBMC. Additionally, allergen-induced IgE-dependent colitis, airway hyperreactivity and mucus-producing goblet cells were significantly inhibited in these mice. Importantly, infiltration of the colon and lung with human CD45+ cells was similar in all groups. These results demonstrate that allergen-specific gut and lung inflammation in PBMC-engrafted humanized mice can be diminished by depletion of NK cells prior to PBMC transfer, which may be of great interest for therapeutic intervention of allergic diseases.
Download or read book Animal Models of T Cell Mediated Skin Diseases written by T. Zollner and published by Springer Science & Business Media. This book was released on 2007-01-19 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pharmaceutical companies are spending increasing amounts of money on drug discovery and development. Nevertheless, attrition rates in clinical development are still very high, and up to 90% of new compounds fail in clinical phase I - III trials, which is partially due to lack of clinical efficacy. This indicates a strong need for highly predictive in vitro and in vivo models. The "50th International Workshop of the Ernst Schering Research Foundation" focussed on "Animal Models of T Cell-Mediated Skin Diseases". Such animal models should have impact not only on inflammatory dermatoses but also on other inflammatory disorders due to their model character. The current volume summarises recent advances in animal research that are important for anti-inflammatory drug discovery.
Download or read book Elucidation of Effector and Regulatory Mechanisms in a Mouse Model of Food induced Gastrointestinal Allergy written by Manja Burggraf and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The prevalence of food allergies has increased in the westernized countries during the past decades. Clinical manifestations of food allergies involve the skin (e.g. atopic dermatitis), the respiratory tract (e.g. rhinitis, and asthma), the ocular area (e.g. conjunctivitis), the gastrointestinal tract (e.g. food-protein-induced enterocolitis syndrome, food-induced proctocolitis, and eosinophilic gastroenteropathies), and the cardiovascular system (e.g. anaphylaxis). A curative treatment of these diseases has not been established yet. Oral immunotherapy (OIT) has gained attention as a potential therapy for food allergies. Continuous feeding of allergenic diet applied in the model described here mirrors to a certain extent an OIT treatment. It might be therefore useful to investigate efficacy and safety of OIT pre-clinically. Mouse models have been widely used to analyse novel treatment approaches. Unfortunately, most of them have focussed on IgE-mediated hyperreactivity. Only a limited number of mouse models presenting mixed IgE- and non-IgE-mediated gastrointestinal symptoms and inflammation upon allergen-challenge are available. To study the mechanisms underlying the induction of food-induced gastrointestinal inflammation and subsequent oral tolerance induction, a mouse model of food-induced gastrointestinal allergy was established. BALB/c mice were sensitised with Ovalbumin (OVA) plus ALUM and subsequently challenged by feeding a diet containing egg white (EW diet). During the first seven days on EW diet, OVA-sensitised mice (OVA/ALUM EW mice) developed gastrointestinal symptoms (e.g. weight loss, ruffed fur, soft stool and less mobility) and inflammation in the small intestines accompanied by a strong induction of OVA-specific IgE antibodies and mouse mast cell protease-1 (mMCP-1). Proliferation of CD4+ T cells from spleen of OVA/ALUM EW mice was reduced compared controls. ...
Download or read book Neuroimmune Interactions in Allergic Airway Diseases written by Duc Dung Le and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Asthma written by Magdalena M. Gorska and published by Springer Nature. This book was released on 2022-06-30 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details a collection of laboratory protocols to study asthma in mice and humans. Chapters cover animal models of asthma, methods to measure asthma-related molecules, protocols to detect, isolate, culture and stimulate cells that contribute to asthma in mice and humans, methods to deplete these cells in vivo and study responses of intact airway tissues ex vivo. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Asthma: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.
Download or read book Investigation of Immune Responses in Different Mouse Models of Allergic Asthma written by Frank Kirstein and published by . This book was released on 2008 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt:
