Download or read book Morphological Comparison of Stem Cells Using Two Dimensional Culture and Spheroid Culture written by Jun-Beom Park and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mesenchymal stem cells are of great interest, especially in regeneration medicine. Mesenchymal stem cells have the ability to differentiate into several tissues including bone and fat. The stem cells can be obtained from various tissues including bone marrow, periosteum, gingiva, and tooth. Traditionally, two-dimensional culture has been applied for stem cell research. However, more recently, a three-dimensional model has been of great interest for studying the stem cells because it mimics the physiological conditions. Spheroid culture is one way of applying three-dimensional culture. This report describes the two-dimensional culture and spheroid culture and the morphological comparison will be performed between two-dimensional culture and spheroid culture.
Download or read book Effect of Dimensionality on in Vitro Growth Environment and Mesenchymal Stem Cell Function written by Fatema Tuj Zohora and published by . This book was released on 2018 with total page 86 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of the standard two dimensional (2D) cell culture has laid down the fundamentals of molecular and cell biology. However, recent advances in cell-based regenerative medicine raises the concern on deconstructing cellular behaviors in more physiologically relevant three dimensional (3D) microenvironments. Differences in cell response in 2D versus 3D systems arise from the perturbations in gene expression patterns that stem from how cells sense their underlying 2D or surrounding 3D matrices and adjust their phenotypes accordingly. Thus, cells are no longer considered as a solitary entity of genome but a context arises from a combinatorial interactions of cell-ECM, cell-cell, and cell-biomolecules that constitutively organize the tissues and ultimate functional organs. In this regard, this project was intended to identify cell behaviors and gene expression pattern in 2D versus 3D culture systems. To construct 2D versus 3D cultures of human adipose tissue-derived mesenchymal stem cells (hASCs) were seeded on the top of plastic substrates or encapsulated in the self-assembled polypeptide-based hydrogel PuraMatrixTM, respectively. Adipogenic, osteogenic, and chondrogenic differentiation in these two different culture systems was induced with the corresponding lineage-specific biochemical induction medium. The effect on growth and differentiation of hASCs under these two culture conditions was assessed through changes in cellular morphology, survivability, proliferation, and gene expression pattern during lineage-specific differentiation. According to the cytotoxicity assay, PuraMatrixTM provides a suitable 3D microenvironment for hASCs proliferation and differentiation without the need of any exogeneous cell adhesion motifs, however an influence of initial cell seeding density was seen in maintaining high initial cell viability and subsequent functionality. Gene microarray analysis revealed that, compared to 2D culture, 3D culture shows more of the expected lineage-specific gene expression and supports tissue specific developmental characteristics.
Download or read book Stem Cell and Tissue Engineering written by Song Li and published by World Scientific. This book was released on 2011 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue engineering integrates knowledge and tools from biological sciences and engineering for tissue regeneration. A challenge for tissue engineering is to identify appropriate cell sources. The recent advancement of stem cell biology provides enormous opportunities to engineer stem cells for tissue engineering. The impact of stem cell technology on tissue engineering will be revolutionary. This book covers state-of-the-art knowledge on the potential of stem cells for the regeneration of a wide range of tissues and organs and the technologies for studying and engineering stem cells. It serves as a valuable reference book for researchers and students.
Download or read book Human Embryonic Stem Cells written by Arlene Chiu and published by Springer Science & Business Media. This book was released on 2003-08 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: A discussion of all the key issues in the use of human pluripotent stem cells for treating degenerative diseases or for replacing tissues lost from trauma. On the practical side, the topics range from the problems of deriving human embryonic stem cells and driving their differentiation along specific lineages, regulating their development into mature cells, and bringing stem cell therapy to clinical trials. Regulatory issues are addressed in discussions of the ethical debate surrounding the derivation of human embryonic stem cells and the current policies governing their use in the United States and abroad, including the rules and conditions regulating federal funding and questions of intellectual property.
Download or read book General Biophysics written by M Volkenstein and published by Elsevier. This book was released on 2012-12-02 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: General Biophysics, Volume I deals with the theoretical physics underlying biological phenomena and presents some pertinent experimental results. It explores the molecular foundations of biophysics, the thermodynamics of nonequilibrium systems and membrane transport, nerve impulses, and mechanochemical processes. Comprised of five chapters, this volume begins with an overview of molecular biophysics and the concept of molecular recognition, followed by a discussion of the interaction between antibodies and antigens, the primary processes that determine odor reception, and the importance of intercellular interactions in the existence and development of multicellular organisms. The next chapters explain how protein biosynthesis is regulated by molecules and how proteins are biosynthesized in eukaryotic cells, along with the application of thermodynamics to the analysis of biophysical problems and the coupling of chemical reactions near equilibrium. The reader is also introduced to the stability conditions of a steady state, the concept of entropy for an open system, the thermodynamics of the sodium pump, ionic equilibrium between sodium and potassium solutions separated by an active membrane, the conformational properties of membranes, and the general phenomenological theory of facilitated transport and the role of the carriers. The book concludes with a chapter on biological mechanochemical processes and their thermodynamics. This book is a valuable resource for physicists and biophysicists, graduate and postgraduate students having the necessary knowledge of physics, and anyone acquainted with proteins and nucleic acids.
Download or read book Cell Culture written by Radwa Ali Mehanna and published by BoD – Books on Demand. This book was released on 2019-01-23 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell culture is one of the major tools used in cellular and molecular biology, delivering an excellent model for studying the normal physiology and biochemistry of cells. This book covers some advanced aspects in cell culture methodologies. The book has four sections discussing different types of cell culture models, including 3D cell culture techniques, their advantages, and limitations in comparison to traditional 2D culturing; cell viability, autophagy, in vitro toxicity tests and live cell imaging; stem cell culture for cell-based therapeutics; and specific applications and methodologies for hybrid cell lines and cancer models. This book provides a comprehensive overview of some of the advanced cell culture methodologies and applications. It serves as a valuable source for scientists, researchers, clinicians and students.
Download or read book Stem Cells written by Christine L. Mummery and published by Academic Press. This book was released on 2014-05-23 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of Stem Cells: Scientific Facts and Fiction provides the non-stem cell expert with an understandable review of the history, current state of affairs, and facts and fiction of the promises of stem cells. Building on success of its award-winning preceding edition, the second edition features new chapters on embryonic and iPS cells and stem cells in veterinary science and medicine. It contains major revisions on cancer stem cells to include new culture models, additional interviews with leaders in progenitor cells, engineered eye tissue, and xeno organs from stem cells, as well as new information on "organs on chips" and adult progenitor cells. In the past decades our understanding of stem cell biology has increased tremendously. Many types of stem cells have been discovered in tissues that everyone presumed were unable to regenerate in adults, the heart and the brain in particular. There is vast interest in stem cells from biologists and clinicians who see the potential for regenerative medicine and future treatments for chronic diseases like Parkinson's, diabetes, and spinal cord lesions, based on the use of stem cells; and from entrepreneurs in biotechnology who expect new commercial applications ranging from drug discovery to transplantation therapies. Explains in straightforward, non-specialist language the basic biology of stem cells and their applications in modern medicine and future therapy Includes extensive coverage of adult and embryonic stem cells both historically and in contemporary practice Richly illustrated to assist in understanding how research is done and the current hurdles to clinical practice
Download or read book Stem Cell Regulators written by and published by Academic Press. This book was released on 2011-11-23 with total page 523 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 1943, Vitamins and Hormones is the longest-running serial published by Academic Press. The Editorial Board now reflects expertise in the field of hormone action, vitamin action, X-ray crystal structure, physiology and enzyme mechanisms. Under the capable and qualified editorial leadership of Dr. Gerald Litwack, Vitamins and Hormones continues to publish cutting-edge reviews of interest to endocrinologists, biochemists, nutritionists, pharmacologists, cell biologists and molecular biologists. Others interested in the structure and function of biologically active molecules like hormones and vitamins will, as always, turn to this series for comprehensive reviews by leading contributors to this and related disciplines. This volume focuses on stem cell regulators. Longest running series published by Academic Press Contributions by leading international authorities
Download or read book Spheroid Culture in Cancer Research 1991 written by Rolf Bjerkvig and published by CRC Press. This book was released on 2017-11-22 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: Spheroid Culture in Cancer Research describes the various techniques now available for establishing spheroid tissue culture, including spheroid culture from normal tissues and from tumor cell lines. The book also describes how the spheroid system can be used to study interactions between normal and malignant cells. Microenvironmental conditions in spheroids and how this micromilieu may promote cellular heterogeneity and histiotypic structures not observed in corresponding monolayer cultures are discussed. The biological importance of oxygen tension, pH gradients, diffusions of nutrients, and cell-cell communication in spheroids are also examined. The book will be profoundly important to researchers in experimental chemotherapy, radiotherapy, immunotherapy, and hyperthermia.
Download or read book Stem Cells written by Ariff Bongso and published by World Scientific. This book was released on 2011 with total page 721 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem cell biology has drawn tremendous interest in recent years as it promises cures for a variety of incurable diseases. This book deals with the basic and clinical aspects of stem cell research and involves work on the full spectrum of stem cells isolated today. It also covers the conversion of stem cell types into a variety of useful tissues which may be used in the future for transplantation therapy. It is thus aimed at undergraduates, postgraduates, scientists, embryologists, doctors, tissue engineers and anyone who wishes to gain some insight into stem cell biology. This book is important as it is comprehensive and covers all aspects of stem cell biology, from basic research to clinical applications. It will have 33 chapters written by renowned stem cell scientists worldwide. It will be up-to-date and all the chapters include self-explanatory figures, color photographs, graphics and tables. It will be easy to read and give the reader a complete understanding and state of the art of the exciting science and its applications.
Download or read book 3D Cell Culture written by Zuzana Koledova and published by Humana Press. This book was released on 2017-06-21 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an overview of established 3D cell culture assays from leaders in the field. Their contributions cover a wide spectrum of techniques and approaches for 3D cell culture, from organoid cultures through organotypic models to microfluidic approaches and emerging 3D bioprinting techniques, which are used in developmental, stem cell, cancer, and pharmacological studies, among many others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, 3D Cell Culture: Methods and Protocols aims to inspire researchers to develop novel 3D cell culture techniques according to their specific scientific needs and interests, leading to a new generation of physiologically relevant and realistic 3D cell cultures. Chapter 15 of this book is available open access under a CC BY 4.0 license.
Download or read book Spheroids in Cancer Research written by H. Acker and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malignant growth of cells is often characterized by disorganization of tissue structure, abnormal blood vessel development, and insuffi cient vascular supply. As a consequence, the cancer cells grow in a three-dimensional pattern in atypical microenvironments which include physical, chemical, and nutritional stresses. Necrosis often develops some distance away from the blood vessels. In association with an inherent instability in malignant cell populations, and also because of the changing micromilieu, significant cellular heteroge neity emerges with regard to various phenotypic characteristics. Both biological behavior and responses to therapeutic agents can be affected. A variety of in vitro and in vivo experimental models exist for research on properties of cancer cells during growth. The multicell spheroid model was developed as a system of intermediate complexity in which three dimensional growth of cells enhances cell-cell interactions and creates micro environments that simulate the conditions in intervascular microregions of tumors or microme tastatic foci. Spheroids may change their cellular characteristics with changing environments during growth. These can be studied under controlled conditions in vitro. Interest in details of experimental methods for this model system stimulated the organization of the First International Conference in Rochester, NY in 1980, the Proceedings of which were summarized in Cancer Research in 1981. Since then there has been a rapid increase in the use of this model system, and increased research on the significance of cell-cell and cell-microenvironment interactions in biology in general.
Download or read book Differentiation of Embryonic Stem Cells written by and published by Elsevier. This book was released on 2003-12-18 with total page 577 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume covers all aspects of embryonic stem cell differentiation, including mouse embryonic stem cells, mouse embryonic germ cells, monkey and human embryonic stem cells, and gene discovery. * Early commitment steps and generation of chimeric mice* Differentiation to mesoderm derivatives* Gene discovery by manipulation of mouse embryonic stem cells
Download or read book New Insights into Cell Culture Technology written by Sivakumar Joghi Thatha Gowder and published by BoD – Books on Demand. This book was released on 2017-05-10 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book "New Insights into Cell Culture Technology" focuses on many advanced methods and techniques concerned with cell culture. The contributing authors have discussed various developments in cell culture methods, the application of insect cells for the efficient production of heterologous proteins, the expansion of human mesenchymal stromal cells for different clinical applications, the remote sensing of cell culture experiments and concepts for the development of cell culture bioprocess, continuous production of retroviral pseudotype vectors, and the production of oncolytic measles virus vectors for cancer therapy. This book is an original contribution of experts from different parts of the globe, and the in-depth information will be a significant resource for students, scientists, and physicians who are directly dealing with cells.["Culture" is essential for human life and also the life of a cell. - Sivakumar Gowder]
Download or read book Stem Cells in Aesthetic Procedures written by Melvin A. Shiffman and published by Springer. This book was released on 2014-07-21 with total page 815 pages. Available in PDF, EPUB and Kindle. Book excerpt: Interest in the use of stem cells in aesthetic procedures has been increasing rapidly, reflecting the widespread acknowledgment of the tremendous potential of stem cell fat transfer. This is, however, the first book to be devoted entirely to the subject. The book opens by reviewing the history of the development of pluripotent stem cells and the results of research into the biochemistry and physiology of stem cells. Adipose tissue anatomy and survival are discussed and the wide range of aesthetic procedures involving stem cell fat transfer are then described in detail. These procedures relate to the face, breast, buttocks, legs, hands, penis and Poland syndrome. In addition, potential risks and complications are identified. The book has been written by leading experts and will be an invaluable source of information for students, beginners and experienced surgeons in a range of specialties.
Download or read book Cell Engineering and Regeneration written by Heinz Redl and published by Springer. This book was released on 2017-02-16 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference work presents the origins of cells for tissue engineering and regeneration, including primary cells, tissue-specific stem cells, pluripotent stem cells and trans-differentiated or reprogrammed cells. There is particular emphasis on current understanding of tissue regeneration based on embryology and evolution studies, including mechanisms of amphibian regeneration. The book covers the use of autologous versus allogeneic cell sources, as well as various procedures used for cell isolation and cell pre-conditioning , such as cell sorting, biochemical and biophysical pre-conditioning, transfection and aggregation. It also presents cell modulation using growth factors, molecular factors, epigenetic approaches, changes in biophysical environment, cellular co-culture and other elements of the cellular microenvironment. The pathways of cell delivery are discussed with respect to specific clinical situations, including delivery of ex vivo manipulated cells via local and systemic routes, as well as activation and migration of endogenous reservoirs of reparative cells. The volume concludes with an in-depth discussion of the tracking of cells in vivo and their various regenerative activities inside the body, including differentiation, new tissue formation and actions on other cells by direct cell-to-cell communication and by secretion of biomolecules.
Download or read book Cutaneous Wounds written by F. Joseph Dagher and published by Blackwell/Futura. This book was released on 1985 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt:
