Download or read book Monitoring Multiple Resistance to Soybean Cyst Nematode SCN Using Molecular Markers in a Breeding Population written by Jennifer Heer and published by . This book was released on 1996 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification and Characterization of Soybean Cyst Nematode Resistance Genes Using DNA Markers written by Vergel Cierte Concibido and published by . This book was released on 1995 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book DNA Markers and Genetics of Resistance to Cyst Nematode and Seed Composition in Soybean Peking X Essex written by Boxing Qiu and published by . This book was released on 1998 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: Soybean [Glycine max (L.) Merr.] is one of the major crops in the USA and worldwide. Soybean cyst nematode (SCN), Heterodera glycines Ichinohe, causes severe damage to soybean production. In 1988, the SCN was ranked as the number one crop disease in the southern USA. To combat this pest, genetic characterization of the resistance genes to SCN and molecular approaches for tagging the resistance genes have recently been conducted. The cultivar 'Peking' is one of the most important resistance sources to SCN. It gives resistance to SCN Race Isolates 1, 3, and 5. The genetic inheritance for resistance to SCN Race 3 in Peking was studied in the past decades, but the resistance nature is complicated and the inheritance patterns for resistance to SCN Races 1 and 5 were not well documented. In this study, F 1, F 2, and F 2:3 subfamilies were used for studying the genetic background of the SCN resistance in Peking. It was discussed that resistance to SCN Races 1, 3, and 5 each were conditioned by a three-gene model, one dominant and two recessives (Rhg, rhg, rhg). The complexity of SCN multiple resistance genes in soybean and the heterogeneity of indigenous field SCN Race populations make it difficult to breed resistant soybean cultivars. Both morphological and molecular markers that are associated with the resistance to SCN are known to play an important role in tagging and transferring resistance genes from wild types into soybean cultivars. Black seed-coat color was previously reported as a morphological marker linked to SCN resistance. Our data indicated that the reddish brown seed-coat color was linked to the loci controlling resistance to SCN Race 3. The mean of the Index of Parasitism (IP) in reddish-brown seed-coat color plants was significantly less than one in the plants with black seed coat color. DNA markers associated with resistance to SCN Race 3 have been documented by a number of scientists. Restriction fragment length polymorphism (RFLP) analysis was used as a molecular marker-mediated approach to identify the markers for SCN resistance in this study. Based on the co-segregation between phenotypic SCN reactions in 200 F 2:3 families and RFLP scoring data from 200 F 2 plants, five DNA markers, A593 and T005 on linkage group (LG) B, A018 in LG E, and K014 and B072 on LG H, were associated with resistance loci for SCN Race 1, which jointly explained 57.7% of the phenotypic variation. Three markers (B072 and K014 on LG H, T005 on LG B) were linked to resistance loci for Race 3, and together explained 21.4% of the total phenotypic variation. Two markers (K011 on LG I, A963 on LG E) associated with resistance to Race 5 jointly explained 14.0% of the total phenotypic variation.
Download or read book Marker assisted Selection for Soybean Cyst Nematode Resistance and Accompanying Agronomic Traits written by Joann Mudge and published by . This book was released on 1999 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic and Molecular Analysis of Soybean Cyst Nematode Virulence written by Michael N. Gardner and published by . This book was released on 2017 with total page 125 pages. Available in PDF, EPUB and Kindle. Book excerpt: The soybean cyst nematode (SCN) Heterodera glycines is the most economically important pathogen of soybean, capable of causing large scale yield loss on a global scale. Current management practices utilize host resistance, but commercial resistance is limited to three main sources, the soybean plant introductions (PI) 54802 (Peking), 88788, and 437654. More than 95% of soybean grown in the north central United States have resistance derived from PI 88788 and repeated use of this resistance has led to the development of highly virulent nematode populations capable of infecting these resistant plants and causing yield losses. In order to identify the mode of inheritance for virulence on the three primary sources of resistance to SCN a controlled crossing study was performed, mating a highly virulent nematode (TN20) with an avirulent nematode (PA3). By monitoring the offspring of this cross it was found that depending on the source of host resistance, virulence is inherited in a dominant or a recessive manner except for virulence on PI 437654, which is likely a multigenic trait dependent on multiple recessive genes. A de novo transcriptome assembly was then generated for SCN and mined for novel stylet-secreted effectors, identifying a new pool of candidates that may play a role in virulence. Finally, a comparative transcriptomic analysis was performed across multiple SCN populations to identify conserved expression patterns and genes associated with virulence. Results from these studies will be used to improve current management practices for SCN and provide new potential targets for improving SCN resistance.
Download or read book Molecular Characterization of Genetic Resistance to Soybean Cyst Nematode in Soybean Line SS97 6946 written by Md Sariful Islam and published by . This book was released on 2008 with total page 45 pages. Available in PDF, EPUB and Kindle. Book excerpt: Soybean Cyst Nematode (SCN) (Heterodera glycine) is the most damaging pest of soybean and estimated annual yield losses are 1.5 billion dollars in USA. Breeding resistant cultivars is the most efficient means to control SCN but the nematode has adapted and overcomes resistance of developed soybean cultivars due to a narrow genetic base. A study was initiated in summer 2007 to investigate the genetics of resistance to SCN and identify Quantitative Trait Loci (QTL) conferring broad-spectrum SCN resistance in SS97-6946. Leaves of 160 F2 individuals from the cross PI 567476 X SS97-6946 were collected to isolate DNA in summer 2007. Three hundred forty seven polymorphic Single Sequence Repeat primer pairs out of 547 were used to genotype the F2 plants. Seeds from 160 F2:3 families were evaluated against races 1, 2, 3 and 5 for SCN bioassay following standard protocol. The ratio observed between resistant to susceptible F2:3 families revealed that SCN resistance involved three recessive genes for both race 1 and 2; two dominant and one recessive for race 3; one dominant and two recessive for race 5. Three markers mapped on linkage groups (LG) A2, E, and G and accounted for 33.8% of the total phenotypic variance for resistance to SCN race 1. One resistant QTL was detected on LG A1 accounted for 18.8% of the total phenotypic variance of race 2. Three markers mapped on LGs A2, G, and M to be associated with resistance to SCN race 3 and shared 24.9% of total phenotypic variance. Three markers on LG G alone and four markers on LGs A1, B2, M, and O were mapped and shown to be linked with SCN resistance to race 5 and accounted for 70.8% of the total phenotypic variance.
Download or read book Biology and Management of the Soybean Cyst Nematode written by Robert D. Riggs and published by American Phytopathological Society. This book was released on 1992 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: 1 History, distribution, and economics. 2 Systematics and morphology. 3 Epiphytology and life cycle. 4 Cellular responses to infection. 5 Population dynamics. 6 Genetics. 7 The race concept. 8 Nematode race identification, A look to the future. 9 Interactions with other organisms. 10 Host range. 11 Chemical control. 12 Management by cultural practices. 13 Biological control. 14 Breeding for resistance to soybean cyst nematode. 15 Cytopathological reactions of resistant soybean plants to nematode invasion. 16 Tolerance in soybean.
Download or read book Identification and Evaluation of Quantitative Trait Loci QTL Associated with Soybean Cyst Nematode HG Type 2 5 7 written by Xin Lu and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Soybean cyst nematode (SCN) is one of the most damaging pests of soybean (Glycine max) worldwide. Currently, more than 95% of SCN-resistant cultivars grown in North America carry the resistance genes from PI88788, which are not resistant to all the Heterodera glycines(HG) types, including HG Type 2.5.7. The objectives of this study were to detect molecular markers associated with the SCN HG Type 2.5.7 and study the relationship between yield and the new resistance genes in two F4-derived recombinant inbred line (RIL) populations derived from crosses between susceptible cultivars OAC Brooke and OAC Calypso and the resistant source, LD07-3419. In total, five quantitative trait loci (QTL) were identified associated with the SCN resistance that explained up to 17% of the phenotypic variation in the populations. In this study, no significant correlation was found between seed yield and the SCN resistance or the detected QTL.
Download or read book Identification and Evaluation of Quantitative Trait Loci Associated with Resistance to HG Type 2 and Seed Quality Traits from Two Bi Parental Mapping Populations Segregating for Soybean Cyst Nematode Resistance written by Adam Brown and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Soybean cyst nematode (SCN), Heterodera glycines Ichinohe, is the most economically devastating pathogen of soybean (Glycine max [L.] Merr.) Overuse of the resistance source PI88788 has led to development of new HG types of SCN in the fields. Major genes, Rhg1 and Rhg4, provide resistance to most HG types. The objectives of this thesis were: 1) identify minor QTL contributing to SCN resistance in two recombinant inbred line populations derived from a cross between the susceptible cultivar, Neptune, and resistant LD07-3419 (PI43764-derived), and between resistant cultivar OAC 13-87C-SCN (PI88788-derived) and LD07-3419 (PI43764); 2) assess the relationship between SCN resistance and yield and seed quality. One of the four identified QTL, which is located on chromosome 6 is potentially novel; however, this result needs to be confirmed. SCN resistance was negatively correlated with protein but not with oil concentration or yield. These findings may facilitate breeding for SCN resistance.
Download or read book Identifying the Genetic Determinants for Virulence in the Soybean Cyst Nematode Heterodera Glycines written by Dave T. Ste-Croix and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The soybean cyst nematode (SCN - Heterodera glycines) is the most economically important pathogen affecting soybean crops, causing significant reductions in yield on a global scale. Currently, the primary method for the management of this destructive root parasite is by utilizing natural host resistance. There are two main sources of genetic resistance commonly used in commercial practices to mitigate these losses: soybean plant introductions (PI) 548402 (Peking) and PI 88788. However, in North America, over 95% of resistant soybeans derive their resistance from PI 88788 genetics. Although still effective to a large extent, prolonged exposure to these limited genetic sources has led to the emergence of virulence within the SCN population, with subpopulations of nematodes now capable of overcoming host resistance. Given that soybean is expected to become one of the most economically significant grain crops in Quebec and Canada, it is crucial to understand how these nematodes overcome resistance. To gain insights into the genetic basis of virulence, a comparative transcriptomic analysis was conducted on individual nematodes isolated from multiple SCN populations with varying degrees of virulence against both main sources of resistance. By comparing the gene expression profiles of females categorized by their virulence phenotypes, we observed a significantly different transcriptomic response in females developing on Peking compared to those developing on PI 88788 or the susceptible control Essex. Indeed, overexpression and repression was observed in multiple effector genes of females developing on Peking. Further sequence analysis of expressed genes in Peking virulent nematodes also revealed a wide array of sequence polymorphisms and differential exon usage not shared by PI 88788 virulent or avirulent nematodes. Building upon the findings of potential alternative splicing in effector genes, a de-novo genome-guided transcriptome was generated in chapter two using long reads sequencing generated from single nematodes. This analysis aimed to assess the presence and extent of alternative splicing within effector genes and, more broadly, the SCN transcriptome. By comparing the expression profiles of these transcripts in PI 88788 virulent and avirulent females from different populations, simultaneously selected on both cultivars, two promising novel effector gene candidates (Hg-CPZ-1 and Hg16414.1) were identified, along with six other overexpressed effector candidates common to all virulent females from PI 88788. Although the two first chapters identified multiple candidate effectors associated with Peking and PI 88788 virulence, the regulatory mechanisms controlling these effectors remained unknown. Consequently, the third chapter explored the SCN microRNAs (miRNA) characterizing candidates potentially involved in the post-transcriptional regulation of effector genes. A comprehensive analysis of whole-nematodes and exosome-derived miRNAs revealed a diverse set of species- and lineage-specific candidates characterized for the first time in the SCN. By utilizing animal-specific and plant-specific miRNA target predictors, a subset of these miRNAs were also predicted to interact with nematode effectors and soybean resistance-related genes emphasizing the complex nature of SCN parasitism through the potential ability of nematodes to not only regulate its effectors genes but also its host genes. In summary, the findings from these chapters have not only enhanced our understanding of the mechanisms underlying the evolution and regulation of effector genes but also provide potential targets for improving resistance against SCN and detecting the presence of this destructive root parasite more effectively.
Download or read book Quantitative Trait Locis Associated with Resistance to Soybean Cyst Nematode in Soybean written by Baohong Guo and published by . This book was released on 2005 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Investigation of Resistance to Heterodea Glycines scn in Soybean Plant Introductions pi 467312 and 507354 written by Peiqin Lu and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Soybean cyst nematode (SCN) Heterodera glycines Ichinohe is the most serious pest of soybean [Glycine max (L.) Merr.] in the world. The effectiveness of breeding soybean SCN resistant cultivars is reduced by the variation of SCN population and narrow genetic basis of resistant soybean cultivars. Hence, it is important to investigate new soybean SCN resistant sources for new genes that confer resistance to SCN field populations such as HG type 1.2.7 to provide durable resistance. Soybean plant introductions PI 467312 and PI 507354, are unique, with resistance to SCN multiple HG types respectively. However, the genetic basis of SCN resistance in these PIs is not known. The objectives of this study are to investigate the inheritance of resistance to SCN HG types 0, 1.2.7, and 1.3.6.7 in PI 467312 and the SCN resistance to SCN HG types 2.5.7 and 1.2.7 in PI 507354, to identify and map quantitative trait loci (QTL) associated with resistance to SCN HG types 0, 1.2.7, 1.3.6.7 in PI 467312 and resistance to SCN HG types 2.5.7, and 1.2.7 in PI 507354. The study showed that resistance to HG types 1.2.7, and 1.3.6.7 in Pop 467 were conditioned by one dominant and two recessive genes (Rhg rhg rhg) and resistance to HG type 0 was controlled by three recessive genes (rhg rhg rhg). Resistance to both HG types 2.5.7 and 1.2.7 in Pop 507 fit a one dominant and 3 recessive gene model (Rhg rhg rhg rhg). Two to three QTLs were associated with resistance to each HG type (race) in both populaitons.
Download or read book Agronomy News written by and published by . This book was released on 1997 with total page 850 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sept.-Oct. issue includes list of theses and dissertations for U.S. and Canadian graduate degrees granted in crop science, soil science, and agronomic science during the previous academic year.
Download or read book Soybean Breeding written by Felipe Lopes da Silva and published by Springer. This book was released on 2017-06-10 with total page 439 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book was written by soybean experts to cluster in a single publication the most relevant and modern topics in soybean breeding. It is geared mainly to students and soybean breeders around the world. It is unique since it presents the challenges and opportunities faced by soybean breeders outside the temperate world.
Download or read book Plant Sciences Reviews 2011 written by David Hemming and published by CABI. This book was released on 2012 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Sciences Reviews 2011 provides scientists and students in the field with timely analysis on key topics in current research. Originally published online in CAB Reviews, this volume makes available in printed form the reviews in plant sciences published during 2011.
Download or read book Marker assisted Selection written by Food and Agriculture Organization of the United Nations and published by Food & Agriculture Org.. This book was released on 2007 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive description and assessment of the use of marker-assisted selection for increasing the rate of genetic gain in crops, livestock, forestry and fish, including the related policy, FAO's tradition of dealing with issues of importance to agricultural and economic development in a multidisciplinary and cross-sectoral manner.
Download or read book Applications and Potentials of Marker Assisted Selection MAS in Plant Breeding written by Sarah Brumlop and published by . This book was released on 2011 with total page 131 pages. Available in PDF, EPUB and Kindle. Book excerpt:
