Download or read book Molecular Methods for the Detection and Characterization of Foodborne and Environmental Pathogens written by Suresh D. Pillai and published by DEStech Publications, Inc. This book was released on 2016-12-02 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt: Techniques for the detection and characterization of microbial pathogens in foods and environmental samples * Molecular techniques and their underlying theories and protocols laid out step by step * Protocols cover: sample processing, DNA extraction, PCR amplification of target genes, methods for quantifying and quality checking DNA/RNA samples * Also presents conventional microbiology, DNA, RNA and other genetic techniques using commercially available materials and kits * Guidance for examining, recording, and interpreting data. Combining the depth of a textbook and the guidance of a lab manual, this volume introduces contemporary molecular methods that are widely used to identify and characterize microbial populations and specific pathogens. The book is structured to explain the basic theory and the rationale for choosing appropriate approaches and specific methods. This material is supplemented by in-depth descriptions of lab experiments. Step-by-step guidelines are provided for a variety of experimental objectives. The techniques explained in this volume can be used in a variety of instructional settings and have been taught to students in academic disciplines such as food science, nutrition, environmental technology, and animal science.
Download or read book Molecular Methods for the Detection and Characterization of Foodborne and Environmental Pathogens written by Suresh D. Pillai and published by . This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Rapid Detection and Characterization of Foodborne Pathogens by Molecular Techniques written by Robert E. Levin and published by CRC Press. This book was released on 2009-10-26 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by one of the most prolific and respected researchers in food safety, this volume describes molecular techniques for the detection and discrimination of major infectious bacteria associated with foods. Each chapter deals with a specific organism and techniques applied to that organism. Particular focus is placed on genes associated with pathogenicity used in the polymerase chain reaction (PCR) including real-time PCR for specific detection of pathogenic bacteria and the inherent limitations of such methodology with certain pathogens. Methods for extracting microorganisms from complex food matrices andDNA purification techniques are also emphasized.
Download or read book Microbiology for Food and Health written by Deepak Kumar Verma and published by CRC Press. This book was released on 2019-12-11 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book, Microbiology for Food and Health: Technological Developments and Advances, highlights the innovative microbiological approaches and advances made in the field of microbial food industries. The volume covers the most recent progress in the field of dairy and food microbiology, emphasizing the current progress, actual challenges, and successes of the latest technologies. This book looks at technological advances in starter cultures, prospective applications of food-grade microorganisms for food preservation and food safety, and innovative microbiological approaches and technologies in the food industry. The first series of chapters discuss the types, classification, and systematic uses of various starter cultures in addition to probiotics for various commercial fermentation processes. The book goes on to covers recent breakthroughs in microbial bioprocessing that can be employed in the food and health industry, such as, for an example, prospective antimicrobial applications of inherently present fermentative microflora against spoilage and pathogenic type microorganisms; the use of potential probiotic LAB biofilms for the control of formation of pathogenic biofilms by exclusion mechanisms, and more.
Download or read book Molecular Detection of Human Bacterial Pathogens written by Dongyou Liu and published by CRC Press. This book was released on 2011-04-18 with total page 1258 pages. Available in PDF, EPUB and Kindle. Book excerpt: As more original molecular protocols and subsequent modifications are described in the literature, it has become difficult for those not directly involved in the development of these protocols to know which are most appropriate to adopt for accurate identification of bacterial pathogens. Molecular Detection of Human Bacterial Pathogens addresses th
Download or read book Industrial and Environmental Biotechnology written by Nuzhat Ahmed and published by Horizon Press. This book was released on 2014-04-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The contamination of the environment by herbicides, pesticides, solvents, various industrial byproducts (including toxic metals, radionucleotides and metalloids) is of enormous economic and environmental significance. Biotechnology can be used to develop "green" or environmentally friendly solutions to these problems by harnessing the ability of bacteria to adapt metabolic pathways, or recruit new genes to metabolise harmful compounds into harmless byproducts. In addition to its role in cleaning-up the environment, biotechnology can be used for the production of novel compounds with both agricultural and industrial applications. Internationally acclaimed authors from diverse fields present comprehensive reviews of all aspects of Industrial and Environmental Biotechnology. Chapters concerned with environmental biotechnology cover two major categories of pollutants: organic compounds and metals. Organic pollutants include cyclic aromatic compounds, with/without nitrogenous or chloride substitutions while metal pollutants include copper, chromate, silver, arsenic and mercury. The genetic basis of bioremediation and the microbial processes involved are examined, and the current and/or potential applications of bioremediation are discussed. The use of biotechnology for industrial and agricultural applications includes a chapter on the use of enzymes as biocatalysts to synthesize novel opiate derivatives of medical value. The conversion of low-value molasses to higher value products by biotechnological methods and the use tissue culture methods to improve sugar cane and potatoes crop production is discussed.
Download or read book Bacteriological Analytical Manual written by United States. Food and Drug Administration. Division of Microbiology and published by . This book was released on 1969 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Foodborne Bacterial Pathogens written by Arnaud Bridier and published by Humana. This book was released on 2018-12-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detection and quantification in food, molecular, phenotypic, metabolic characterization of food pathogens, and ecology of foodborne bacterial pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Foodborne Bacterial Pathogens : Methods and Protocols aims to server as a guide both for researchers, students, and those in the food industry who want to have an overview of current approaches and protocols used to study bacterial foodborne pathogens.
Download or read book Indicators for Waterborne Pathogens written by National Research Council and published by National Academies Press. This book was released on 2004-06-19 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent and forecasted advances in microbiology, molecular biology, and analytical chemistry have made it timely to reassess the current paradigm of relying predominantly or exclusively on traditional bacterial indicators for all types of waterborne pathogens. Nonetheless, indicator approaches will still be required for the foreseeable future because it is not practical or feasible to monitor for the complete spectrum of microorganisms that may occur in water, and many known pathogens are difficult to detect directly and reliably in water samples. This comprehensive report recommends the development and use of a "tool box" approach by the U.S Environmental Protection Agency and others for assessing microbial water quality in which available indicator organisms (and/or pathogens in some cases) and detection method(s) are matched to the requirements of a particular application. The report further recommends the use of a phased, three-level monitoring framework to support the selection of indicators and indicator approaches.Â
Download or read book Microbiology Laboratory Guidebook written by United States. Food Safety and Inspection Service. Microbiology Division and published by . This book was released on 1998 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Dictionary of Microbiology Molecular Biology written by Paul Singleton and published by . This book was released on 2007-11-12 with total page 908 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Third, Revised Edition of a unique, encyclopaedic reference work covers the whole field of pure and applied microbiology and microbial molecular biology, from A to Zythia.
Download or read book Biosensing Technologies for the Detection of Pathogens written by Toonika Rinken and published by BoD – Books on Demand. This book was released on 2018-03-21 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid multiplex detection of pathogens in the environment and in our food is a key factor for the prevention and effective treatment of infectious diseases. Biosensing technologies combining the high selectivity of biomolecular recognition and the sensitivity of modern signal detection platforms are a prospective option for automated analyses. They allow rapid detection of single molecules as well as cellular substances. This book, including 12 chapters from 50 authors, introduces the principles of identification of specific pathogen biomarkers along with different biosensor-based technologies applied for pathogen detection.
Download or read book Current Topics in Salmonella and Salmonellosis written by Mihai Mares and published by BoD – Books on Demand. This book was released on 2017-04-05 with total page 285 pages. Available in PDF, EPUB and Kindle. Book excerpt: The genus Salmonella comprises an important number of bacterial species able to colonize and infect numerous animal species and humans. Although more than a hundred years passed since its discovery, Salmonella still represents a redoubtable and successful microorganism, difficult to deal with. Whether we discuss about typhoid fever or food poisoning, the public health and financial consequences are practically incalculable. The costs attributable to Salmonella contamination of meat, eggs, and vegetables are also very high worldwide. Antimicrobial resistance in Salmonella isolates is an emerging threat not only in humans, and special measures should be addressed to this global problem. The book Current Topics in Salmonella and Salmonellosis contains a series of reviews about all-important issues concerning these subjects. It comprises 14 chapters grouped in 4 sections emphasizing new insights into pathogenesis, bacterial detection and antibiotic resistance, infections in animals, risk factors, and control strategies. The new genomic data and the exhaustive presentation of molecular pathogenesis bring novelty to the book and can help to improve our knowledge about Salmonella-induced diseases.
Download or read book Electrochemical DNA Biosensors written by Mehmet Sengun Ozsoz and published by CRC Press. This book was released on 2012-04-23 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the basic electrochemical applications of DNA in various areas, from basic principles to the most recent discoveries. The book comprises theoretical and experimental analysis of various properties of nucleic acids, research methods, and some promising applications. The topics discussed in the book include electrochemical detection of DNA hybridization based on latex/gold nanoparticle and nanotubes; nanomaterial-based electrochemical DNA detection; electrochemical detection of microorganism-based DNA biosensors; gold nanoparticle-based electrochemical DNA biosensors; electrochemical detection of the aptamer-target interaction; nanoparticle-induced catalysis for DNA biosensing; basic terms regarding electrochemical DNA (nucleic acids) biosensors; screen-printed electrodes for electrochemical DNA detection; application of field-effect transistors to label free electrical DNA biosensor arrays; and electrochemical detection of nucleic acids using branched DNA amplifiers.
Download or read book Identifying Future Drinking Water Contaminants written by National Research Council and published by National Academies Press. This book was released on 1999-09-30 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: With an increasing population, use of new and diverse chemicals that can enter the water supply, and emergence of new microbial pathogens, the U.S. federal government is faced with a regulatory dilemma: Where should it focus its attention and limited resources to ensure safe drinking water supplies for the future? Identifying Future Drinking Water Contaminants is based on a 1998 workshop on emerging drinking water contaminants. It includes a dozen papers that were presented on new and emerging microbiological and chemical drinking water contaminants, associated analytical and water treatment methods for their detection and removal, and existing and proposed environmental databases to assist in their proactive identification and regulation. The papers are preceded by a conceptual approach and related recommendations to EPA for the periodic creation of future Drinking Water Contaminant Candidate Lists (CCLsâ€"produced every five yearsâ€"include currently unregulated chemical and microbiological substances that are known or anticipated to occur in public water systems and that may pose health risks).
Download or read book Foodborne Pathogens written by Joshua B. Gurtler and published by Springer. This book was released on 2018-08-12 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Foodborne illnesses continue to be a major public health concern. All members of a particular bacterial genera (e.g., Salmonella, Campylobacter) or species (e.g., Listeria monocytogenes, Cronobacter sakazakii) are often treated by public health and regulatory agencies as being equally pathogenic; however, this is not necessarily true and is an overly conservative approach to ensuring the safety of foods. Even within species, virulence factors vary to the point that some isolates may be highly virulent, whereas others may rarely, if ever, cause disease in humans. Hence, many food safety scientists have concluded that a more appropriate characterization of bacterial isolates for public health purposes could be by virotyping, i.e., typing food-associated bacteria on the basis of their virulence factors. The book is divided into two sections. Section I, “Foodborne Pathogens and Virulence Factors,” hones in on specific virulence factors of foodborne pathogens and the role they play in regulatory requirements, recalls, and foodborne illness. The oft-held paradigm that all pathogenic strains are equally virulent is untrue. Thus, we will examine variability in virulence between strains such as Listeria, Salmonella, Campylobacter, Cronobacter, etc. This section also examines known factors capable of inducing greater virulence in foodborne pathogens. Section II, “Foodborne Pathogens, Host Susceptibility, and Infectious Dose” , covers the ability of a pathogen to invade a human host based on numerous extraneous factors relative to the host and the environment. Some of these factors include host age, immune status, genetic makeup, infectious dose, food composition and probiotics. Readers of this book will come away with a better understanding of foodborne bacterial pathogen virulence factors and pathogenicity, and host factors that predict the severity of disease in humans.
Download or read book Molecular Techniques in Food Biology written by Aly Farag El Sheikha and published by John Wiley & Sons. This book was released on 2018-01-02 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Techniques in Food Biology: Safety, Biotechnology, Authenticity & Traceability explores all aspects of microbe-food interactions, especially as they pertain to food safety. Traditional morphological, physiological, and biochemical techniques for the detection, differentiation, and identification of microorganisms have severe limitations. As an alternative, many of those responsible for monitoring food safety are turning to molecular tools for identifying foodborne microorganisms. This book reviews the latest molecular techniques for detecting, identifying, and tracing microorganisms in food, addressing both good foodborne microbes, such as those used for fermentation and in probiotics, and harmful ones responsible for foodborne illness and food quality control problems. Molecular Techniques in Food Biology: Safety, Biotechnology, Authenticity & Traceability brings together contributions by leading international authorities in food biology from academe, industry, and government. Chapters cover food microbiology, food mycology, biochemistry, microbial ecology, food biotechnology and bio-processing, food authenticity, food origin traceability, and food science and technology. Throughout, special emphasis is placed on novel molecular techniques relevant to food biology research and for monitoring and assessing food safety and quality. Brings together contributions from scientists at the leading edge of the revolution in molecular food biology Explores how molecular techniques can satisfy the dire need to deepen our understanding of how microbial communities develop in foods of all types and in all forms Covers all aspects of food safety and hygiene, microbial ecology, food biotechnology and bio-processing, food authenticity, food origin traceability, and more Fills a yawning gap in the world literature on food traceability using molecular techniques This book is an important working resource for professionals in agricultural, food science, biomedicine, and government involved in food regulation and safety. It is also an excellent reference for advanced students in agriculture, food science and food technology, biochemistry, microbiology, and biotechnology, as well as academic researchers in those fields.
