Download or read book DNA Markers written by Gustavo Caetano-Anollés and published by Wiley-Liss. This book was released on 1997-09-16 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the four decades since the discovery of DNA, molecular genetics and biotechnology have undergone a revolution in the research and application of genetic testing techniques. As a result, these fields have witnessed an explosion of both information and controversy, and the focus of public awareness of the significance of molecular biotechnology in everyday life has leaped from journal abstracts to front-page news. Molecular biotechnology provides important tools for the identification of specific nucleic acid sequences, some of them linked to important traits such as those conferring resistance to disease. Such DNA markers are used commonly in genetic research and in other applications, such as DNA fingerprinting. Researchers can use DNA markers to follow individual traits in different environments and hosts, increasing our understanding of the constitution, diversity, and evolution of genetic material. DNA Markers: Protocols, Applications, and Overviews offers valuable new information on this rapidly growing field. This compendium of research highlights the most recently discovered DNA markers and their application to the analyses of genomes of low and high complexity, with a focus on recently developed DNA marker systems as tools for DNA fingerprinting, genome analysis, molecular systematics, taxonomy, and phylogenetic analysis. The Protocols section of the book contains detailed, step-by-step instructions on how to perform selected DNA marker experiments, providing a useful laboratory tool. Subjects covered by top specialists include: Designed as a bench-top reference for the laboratory as well as a complete guide to the latest research, DNA Markers: Protocols, Applications, and Overviews is a vital resource for both novices and experienced practitioners involved in research in molecular genetics or biotechnology. A global overview of DNA markers * Molecular hybridization * RAPD, AP-PCR, DAF and AFLP analysis * Microbial Genomic fingerprinting * Simple sequence repeat DNA markers * Genetic BIT analysis * Differential display of RNA * The status of DNA fingerprinting: population databases * Arbitrarily amplified DNA in ecology and evolution

Download or read book Molecular and Biochemical Marker for Fingerprinting written by T. J. Anatala and published by LAP Lambert Academic Publishing. This book was released on 2015-02-10 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cowpea (Vigna unguiculata L.) vegetable plays a vital role in the health and nutritional security of human being and it belongs to the family of fabaceae, is a diploid with chromosome number 2n=22 and genome size is 600 Mb. Cowpea is one of the oldest source of human food.The use of various biochemical techniques like isoenzyme patterns and various molecular marker techniques which are independent of environmental factors like RAPD, ISSR, SSR and protein-profiling using gel electrophoresis techniques offers significant advantage for species identification in that they are rapid, relatively cheap and eliminate the need to grow plants up to maturity. The biochemical methods have some disadvantages e.g. that they are profoundly influenced by tissue specificity and developmental stage. The mapping of genes to control the traits of economic importance will ultimately level to the unraveling of the molecular basis of genetic variation. The main prerequisite for mapping of unknown genes is sufficient number of highly polymorphic marker loci, which are evenly distributed along the chromosome.

Download or read book DNA Fingerprinting in Plants written by Kurt Weising and published by CRC Press. This book was released on 2005-02-28 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: Given the explosive development of new molecular marker techniques over the last decade, newcomers and experts alike in the field of DNA fingerprinting will find an easy-to-follow guide to the multitude of techniques available in DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition. Along with step-by-step annotated p

Download or read book Molecular Markers Natural History and Evolution written by J. C. Avise and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular approaches have opened new windows on a host of ecological and evolutionary disciplines, ranging from population genetics and behavioral ecology to conservation biology and systematics. Molecular Markers, Natural History and Evolution summarizes the multi-faceted discoveries about organisms in nature that have stemmed from analyses of genetic markers provided by polymorphic proteins and DNAs. The first part of the book introduces rationales for the use of molecular markers, provides a history of molecular phylogenetics, and describes a wide variety of laboratory methods and interpretative tools in the field. The second and major portion of the book provides a cornucopia of biological applications for molecular markers, organized along a scale from micro-evolutionary topics (such as forensics, parentage, kinship, population structure, and intra-specific phylogeny) to macro-evolutionary themes (including species relationships and the deeper phylogenetic structure in the tree of life). Unlike most prior books in molecular evolution, the focus is on organismal natural history and evolution, with the macromolecules being the means rather than the ends of scientific inquiry. Written as an intellectual stimulus for the advanced undergraduate, graduate student, or the practicing biologist desiring a wellspring of research ideas at the interface of molecular and organismal biology, this book presents material in a manner that is both technically straightforward, yet rich with concepts and with empirical examples from the world of nature.

Download or read book Development And Analysis Of Molecular Markers For Certain Endangered Species Of Tylophora written by Sebastian.V.A. and published by Archers & Elevators Publishing House. This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Markers for Genebank Management written by David M. Spooner and published by Bioversity International. This book was released on 2005 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt: Overview of molecular technologies. Genebank management. Crop breeding.

Download or read book Focus on DNA Fingerprinting Research written by Marina May Read and published by Nova Publishers. This book was released on 2006 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA Fingerprinting is a method of identification that compares fragments of deoxyribonucleic acid (DNA). It is sometimes called DNA typing. DNA is the genetic material found within the cell nuclei of all living things. The techniques used in DNA fingerprinting also have applications in law and law enforcement, palaeontology, archaeology, various fields of biology, and medical diagnostics. In biological classification, it can help to show evolutionary change and relationships on the molecular level, and it has the advantage of being able to be used even when only very small samples are available. This new book details several applications of this break-through technique.

Download or read book Trends in DNA Fingerprinting Research written by M. M. Reade and published by Nova Publishers. This book was released on 2005 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA Fingerprinting is a method of identification that compares fragments of deoxyribonucleic acid (DNA). It is sometimes called DNA typing. DNA is the genetic material found within the cell nuclei of all living things. The techniques used in DNA fingerprinting also have applications law and law enforcement, palaeontology, archaeology, various fields of biology, and medical diagnostics. In biological classification, it can help to show evolutionary change and relationships on the molecular level, and it has the advantage of being able to be used even when only very small samples are available. This book details several applications of this break-through technique.

Download or read book Molecular Markers in Mycology written by Bhim Pratap Singh and published by Springer. This book was released on 2017-01-21 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Kingdom fungi encompass a massive diversity of taxa with wide-ranging ecologies, life cycles, and morphologies ranging from unicellular aquatic chytrids to large mushrooms. Before molecular methods came in existence, taxonomists considered this Kingdom to be a member of the plant kingdom due to certain life styles like immobility and growth habitats. Molecular markers (also known as DNA markers), facilitated a better alternative method over traditional morphological methods, employed for the identification, characterization, and to understand the evolution of fungi. The morphological methods used for identification are mainly dependent on spore color or microscopic features whereas molecular markers are based on DNA polymorphism in the genomic organization. Phylogenetic studies reported in last decade, based on molecular markers, have reshaped the classification system of Kingdom fungi, which divided into one subkingdom, seven phyla, and ten subphyla. Recent advances in molecular mycology have opened the way for researchers to identify and characterize novel fungal species from unique environments. Mycology is concerned with the systematic study of fungi, including their genetic and biochemical properties, their use to humans as a source of medicine and food, as well as their dangers, such as poisoning and infections. In the 21st century with the development of DNA sequencing technologies and phylogenetic analysis based on molecular markers, new insights into fungal taxonomy were provided. This book contains a thorough discussion of molecular characterization and detection of different groups of fungi by using PCR-based markers and provides a comprehensive view of the applications and uses of different molecular markers in molecular mycology. It also addresses the recent molecular markers employed to solve the problems of identification and discusses current approaches used in molecular characterization and detection of fungi.

Download or read book Molecular and Biochemical Fingerprinting of Clusterbean written by Sampat R. Kalaskar and published by LAP Lambert Academic Publishing. This book was released on 2014-08-29 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular and biochemical fingerprinting used to identification of superior genotypes are the basic objectives of any crop improvement program. The recently developed molecular markers and isoenzymes assay has opened up new visits for the assessment of fingerprinting among genotypes at molecular and biochemical level. In the present investigation, one multi loci molecular markers viz. RAPD (19 Primers), one single locus molecular markers viz. SSR (19 Primers) and three isoenzyme assay viz. PO, PPO and PAL were used to assess the fingerprinting among 12 genotypes of clusterbean, which includes six resistance and six susceptible to bacterial leaf blight genotypes. Fingerprinting analysis was done with the help of statistical analysis such as marker index, polymorphism information content, dendogram and pi-chart. The present study clearly elucidated the RAPD, SSR and isoenzyme assay could be used as a good tool for detecting fingerprinting and can be precisely used for grouping and selection of diverse parents. From the present study like GG-2, HG-75 and HG-365 and GAUG-0522 may be utilized for breeding good genotypes with high yield and resistance to bacterial blight in clusterbean.

Download or read book Environmental Forensics written by R E Hester and published by Royal Society of Chemistry. This book was released on 2008-06-27 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: 'Environmental forensics' is a combination of analytical and environmental chemistry, which is useful in the court room context. It therefore involves field analytical studies and both data interpretation and modelling connected with the attribution of pollution events to their causes. Recent decades have seen a burgeoning of legislation designed to protect the environment and, as the costs of environmental damage and clean-up are considerable, not only are there prosecutions by regulatory agencies, but the courts are also used as a means of adjudication of civil damage claims relating to environmental causes or environmental degradation. As a result is the increasing number of prosecutions of companies who have breached regulations for environmental protection and in civil claims relating to harm caused by excessive pollutant releases to the environment. Such cases can become extremely protracted as expert witnesses provide their sometimes conflicting interpretations of environmental measurement data and their meaning. It is in this context that environmental forensics is developing as a specialism, leading to greater formalisation of investigative methods which should lead to more definitive findings and less scope for experts to disagree. Now a significant subject in its own right, at least one journal devoted to the field and a number of degree courses have sprung up. As a result of the topicality and rapid growth of the subject area, is the publication of this book - the 26th volume in the highly acclaimed Issues in Environmental Science and Technology Series. This volume contains authoritative articles by a number of the leading practitioners across the globe in the environmental forensics field and aims to cover some of the main techniques and areas to which environmental forensics are being applied. The content is comprehensive and describes a number of the key areas within environmental forensics - topics covered by the authors include: - Source identification issues - Microbial techniques - Metal contamination and methods of assigning liability - The use of isotopes to determine sources and their applications - Molecular biological methods - Hydrocarbon fingerprinting techniques - Oil chemistry and key compound identification - The emerging role of environmental forensics in groundwater pollution Additionally, the volume considers specific pollutants and long-lived pollutants of groundwater such as halocarbons which have presented particular problems and which are described in some depth, as well as the way in which chemical degradation processes can lead to compositional changes which provide valuable information. The book provides a comprehensive overview of many of the key areas of environmental forensics written by some of the leading experts in the field. It will be both of specialist use to those seeking expert insights into the field and its capabilities as well as of more general interest to those involved in both environmental analytical science and environmental law.

Download or read book Fingerprinting Analysis and Quality Control Methods of Herbal Medicines written by Ravindra Kumar Pandey and published by CRC Press. This book was released on 2018-07-03 with total page 202 pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to the increase in the consumption of herbal medicine, there is a need to know which scientifically based methods are appropriate for assessing the quality of herbal medicines. Fingerprinting has emerged as a suitable technique for quality estimation. Chemical markers are used for evaluation of herbal medicines. Identification and quantification of these chemical markers are crucial for quality control of herbal medicines. This book provides updated knowledge on methodology, quality assessment, toxicity analysis and medicinal values of natural compounds.

Download or read book DNA Fingerprinting in Plants written by Kurt Weising and published by CRC Press. This book was released on 2014-02-01 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: Given the explosive development of new molecular marker techniques over the last decade, newcomers and experts alike in the field of DNA fingerprinting will find an easy-to-follow guide to the multitude of techniques available in DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition. Along with step-by-step annotated protocols, the authors fully discuss the technical aspects and modifications of existing techniques, the influence of reaction components and conditions, and the analysis of the results. This second edition has been completely revised to address the exponential changes in the field since the first edition, focusing on PCR-based techniques but also including more sophisticated ones. The authors include numerous case studies to illustrate applications of the methods, more than 1600 references to the literature, and descriptions of reagent formulation, equipment, and computer programs used for evaluating molecular marker data. They compare the various methods, including the costs and benefits of each, helping readers determine which is best suited to a particular application. The well-rounded, cross-referenced, and unified nature of this book makes it intrinsically easier to follow than the edited, multi-authored books currently available. It is an absolute necessity on the lab bench of anyone involved in plant research, DNA profiling, and molecular markers.

Download or read book Predicting Structured Data written by Neural Information Processing Systems Foundation and published by MIT Press. This book was released on 2007 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt: State-of-the-art algorithms and theory in a novel domain of machine learning, prediction when the output has structure.

Download or read book Molecular Applications in Biological Anthropology written by Eric Jeffrey Devor and published by Cambridge University Press. This book was released on 1992-12-17 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents the findings of a selection of pioneering research studies in which new molecular techniques have been used to address key questions in biological anthropology, for example about the human genetic system, the geographical movements of human populations in the past, and primate evolution.

Download or read book Food Hygiene Agriculture And Animal Science Proceedings Of The 2015 International Conference written by Hongbiao Ding and published by World Scientific. This book was released on 2016-03-04 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Proceedings of the 2015 International Conference on Food Hygiene, Agriculture and Animal Science provides an all-encompassing review of each contributor's study in topics such as food hygiene, agriculture, animal science, animal histology and embryology, and livestock production systems.This book is not only a compilation and analysis of the existing theories and findings; it also places a strong emphasis on new investigations and experiments. Researchers, engineers, academics and industry professionals in the fields of agricultural science, food hygiene and animal science will find this book a valuable read and useful reference.

Download or read book DNA based markers in plants written by R.L. Phillips and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt: The double helix architecture of DNA was elucidated in 1953. Twenty years later, in 1973, the discovery of restriction enzymes helped to create recombinant DNA molecules in vitro. The implications of these powerful and novel methods of molecular biology, and their potential in the genetic manipulation and improvement of microbes, plants and animals, became increasingly evident, and led to the birth of modern biotechnology. The first transgenic plants in which a bacterial gene had been stably integrated were produced in 1983, and by 1993 transgenic plants had been produced in all major crop species, including the cereals and the legumes. These remarkable achievements have resulted in the production of crops that are resistant to potent but environmentally safe herbicides, or to viral pathogens and insect pests. In other instances genes have been introduced that delay fruit ripening, or increase starch content, or cause male sterility. Most of these manipulations are based on the introduction of a single gene - generally of bacterial origin - that regulates an important monogenic trait, into the crop of choice. Many of the engineered crops are now under field trials and are expected to be commercially produced within the next few years. The early successes in plant biotechnology led to the realization that further molecular improvement of plants will require a thorough understanding of the molecular basis of plant development, and the identification and character ization of genes that regulate agronomically important multi genic traits.