Download or read book Microtubule Assembly Dynamics in Vivo written by Lynne U. Cassimeris and published by . This book was released on 1988 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Laser Mediated Gene Transfer and Dynamics of Microtubule Assembly in Vivo written by Wen Tao and published by . This book was released on 1988 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microtubule Assembly in Vivo written by Bohdan J. Soltys and published by . This book was released on 1988 with total page 602 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microtubules in vitro written by John J. Correia and published by Academic Press. This book was released on 2013-08-20 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This Second Edition brings together current research and protocols in the field of microtubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors. Chapters reflect experimental procedures and new developments in the field of microtubule in vitro research Combines classical approaches and modern technologies Presents easy-to-use protocols and thorough background information, compiled by leaders in the field
Download or read book Microtubules in vitro written by John J. Correia and published by Academic Press. This book was released on 2010-07-03 with total page 722 pages. Available in PDF, EPUB and Kindle. Book excerpt: There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This volume (complemented by the forthcoming companion volume by Cassimeris and Tran) brings together current research and protocols in the field of microtoubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors. Chapters reflect both experimental procedures and new developments in the field of microtubule in vitro research Combines classical approaches and modern technologies Presents easy-to-use protocols and thorough background information, compiled by leaders in the field
Download or read book Microtubule Dynamics written by Anne Straube and published by Humana Press. This book was released on 2017-04-30 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In Microtubule Dynamics: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics in vitro and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range of well-established and newly-emerging techniques in this vital field.
Download or read book Tubulin and Microtubule Dynamics in Vivo written by William M. Saxton and published by . This book was released on 1986 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microtubules in vivo written by and published by Academic Press. This book was released on 2010-09-24 with total page 549 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microtubules: in vivo includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeleton. The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation. Includes chapters by experts around the world on many aspects of microtubule imaging in living and fixed cells; assays to study microtubule function in a wide array of model organisms and cultured cells; high resolution approaches to study of the cytoskeleton The authors share their years of experience, outlining potential pitfalls and critical factors to consider in experimental design, experimental implementation and data interpretation
Download or read book The Control of Microtubule Assembly in Vivo written by E.C. Raff and published by . This book was released on 1979 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book In Vivo Microtubule Dynamics in Reticulomyxa and Small Molecular Weight GTP Binding Proteins in Secretion written by Yih-Tai Chen and published by . This book was released on 1993 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of Microtubule Assembly in Vivo written by Bonnie J. Howell and published by . This book was released on 1998 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: These studies identify Op18 as a unique regulator of microtubule assembly and have led me to propose a novel mechanism through which Op18 regulates microtubule assembly. Furthermore, these studies suggest Op18 is a likely target in signaling cascades that modulate changes in microtubule behavior and result in changes in cellular morphology.
Download or read book Cytoskeleton written by Jose C. Jimenez-Lopez and published by BoD – Books on Demand. This book was released on 2017-05-17 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: The cytoskeleton is a highly dynamic intracellular platform constituted by a three-dimensional network of proteins responsible for key cellular roles as structure and shape, cell growth and development, and offering to the cell with "motility" that being the ability of the entire cell to move and for material to be moved within the cell in a regulated fashion (vesicle trafficking). The present edition of Cytoskeleton provides new insights into the structure-functional features, dynamics, and cytoskeleton's relationship to diseases. The authors' contribution in this book will be of substantial importance to a wide audience such as clinicians, researches, educators, and students interested in getting updated knowledge about molecular basis of cytoskeleton, such as regulation of cell vital processes by actin-binding proteins as cell morphogenesis, motility, their implications in cell signaling, as well as strategies for clinical trial and alternative therapies based in multitargeting molecules to tackle diseases, that is, cancer.
Download or read book Regulation of Microtubule Dynamics by Saccharomyces Cerevisiae Plus end Tracking Proteins written by Kristina Anne Blake-Hodek and published by . This book was released on 2010 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microtubule dynamics are regulated by a variety of proteins that bind to microtubule ends, and influence their polymerization properties. A number of these plus-end binding proteins have been identified and the interactions between these proteins are important for regulating microtubule dynamics. Stu2, Bik1, and Bim1 are three microtubule plus-end tracking proteins in Saccharomyces cerevisiae and each has been shown to influence microtubule assembly in vivo. I have found that Stu2, Bik1, and Bim1 interact both with themselves and each other in all pairwise combinations. Mapping of protein-protein interaction domains, competitive interaction assays, and physical characterization of protein complexes indicate that these proteins do not associate in a single complex but rather compete for binding to each other. Overall, these results suggest that the interactions among these proteins are dynamic and that different complexes of these proteins may perform distinct roles in the cell. In order to further characterize these proteins, I purified Bim1 and Bik1. I show that in vitro Bim1 and Bik1 form homodimers that can interact with each other to form a tetramer, indicating the Bim1-Bik1 interaction is direct. Using purified GFP tagged versions of these proteins, I observed Bim1 to localize directly to the microtubule plus-end, however, Bik1 required Bim1 for efficient plus-end localization. I also examined the effects of Bim1 and Bik1 on MT assembly and dynamics in vitro. I found that Bim1 has a stabilizing effect on microtubules resulting in an increase of polymer formation. This effect is primarily due to the suppression of microtubule catastrophes by Bim1, although Bim1 also increases microtubule growth rates and rescue frequencies. The ability of Bim1 to promote MT polymerization is likely due to a direct effect of Bim1 on MT plus-end structure as I found that Bim1 does not bind tubulin subunits. In contrast, Bik1 had a destabilizing effect on microtubules, increasing the frequency of catastrophes. This may be due to the formation of polymerization-incompetent tubulin oligomers as I observed Bik1 to promote oligomerization of tubulin subunits. Combining equal-molar amounts of Bim1 and Bik1 resulted in an effect on microtubule dynamics similar to Bim1 alone. These results indicate that Bim1 is able to suppress the catastrophe promoting activities of Bik1 and could be due to formation of a Bim1-Bik1 complex that disrupts Bik1-tubulin interactions. In agreement with this theory, Bik1 is known to bind both Bim1 and tubulin through the same domain. Overall, these results add to our knowledge of plus-end tracking proteins and the individual method each protein uses to influence MT dynamics.
Download or read book Live Cell Imaging written by Robert D. Goldman and published by CSHL Press. This book was released on 2005 with total page 658 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
Download or read book Cytoskeleton Dynamics written by Helder Maiato and published by . This book was released on 2020 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details comprehensive state-of-the-art methods on actin microfilaments and microtubules and how they work to achieve different cellular functions in different cellular contexts. Chapters guide readers through protein purification, in vitro reconstitution of several cytoskeleton properties, analyses of microtubule- and actin-based structures, functional dissection of post-translational modifications, and roles in several biological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cytoskeleton Dynamics: Methods and Protocols aims to provide a wide range of experimental approaches and be an invaluable resource for present and future generations of cytoskeleton researchers. .
Download or read book Microtubule Accessory Proteins and Microtubule Assembly in Vivo written by Joseph Anthony Connolly and published by c1979.. This book was released on 1979 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt:
