Download or read book Microautoradiography and Electron Probe Analysis written by U. Lüttge and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: In biological literature, several definitions of quantitative autoradio graphy are given. The term is defined as either the determination and com parison of the density of silver grains above various structures or under varying conditions, or the determination of absolute quantities of radio activity. In both these cases, photometric measurement serves for more rapid and more exact evaluation of grain densities than would be possible by visual counting of the grains. The equipment generally used for the photometric measurement of silver grains consists of a microscope, a photocell, an electronic amplifier system and a display unit. Grains can be made accessible to photometric evaluation by various kinds of microscopic illumination: 1. Substage bright-field illumination. 2. Substage dark-field illumination. 3. Incident dark-field illumination. 4. Vertical bright-field illumination. With all these types of illumination, the relationship between the luminous flux I absorbed by the film, scattered into the objective and reflected or diffracted, and the flux 10 which is not affected by the film is used as a measure of grain density. Since these are differential measurements, the light beam I transmitted by the film is in itself a measure of grain density if the luminous flux 10 incident on the grains is kept constant. This approach has been used in a large number of measuring setups.
Download or read book Microautoradiography and Electron Probe Analysis written by Wolfgang O. Abel and published by Springer. This book was released on 1972 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microautoradiography and Electron Probe Analysis written by Ulrich Lüttge and published by . This book was released on 1972-12-28 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Progress of Electron Probe Micro analysis written by Robert Frederick Symes and published by . This book was released on 1976 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electron Probe Microanalysis written by Karl Zierold and published by Springer Science & Business Media. This book was released on 2013-03-08 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of electron probe microanalysis of biological systems is to identify, localize, and quantify elements, mass, and water in cells and tissues. The method is based on the idea that all electrons and photons emerging from an electron beam irradiated specimen contain information on its structure and composition. In particular, energy spectroscopy of X-rays and electrons after interaction of the electron beam with the specimen is used for this purpose. However, the application of this method in biology and medicine has to overcome three specific problems: 1. The principle constituent of most cell samples is water. Since liquid water is not compatible with vacuum conditions in the electron microscope, specimens have to be prepared without disturbing the other components, in parti cular diffusible ions (elements). 2. Electron probe microanaly sis provides physical data on either dry specimens or fully hydrated, frozen specimens. This data usually has to be con verted into quantitative data meaningful to the cell biologist or physiologist. 3. Cells and tissues are not static but dynamic systems. Thus, for example, microanalysis of physiolo gical processes requires sampling techniques which are adapted to address specific biological or medical questions. During recent years, remarkable progress has been made to overcome these problems. Cryopreparation, image analysis, and electron energy loss spectroscopy are key areas which have solved some problems and offer promise for future improvements.
Download or read book Quantitative Electron Microprobe Analysis written by Roger Theisen and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 173 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electron Probe Quantitation written by K.F.J. Heinrich and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 1968, the National Bureau of Standards (NBS) published Special Publication 298 "Quantitative Electron Probe Microanalysis," which contained proceedings of a seminar held on the subject at NBS in the summer of 1967. This publication received wide interest that continued through the years far beyond expectations. The present volume, also the result of a gathering of international experts, in 1988, at NBS (now the National Institute of Standards and Technology, NIST), is intended to fulfill the same purpose. After years of substantial agreement on the procedures of analysis and data evaluation, several sharply differentiated approaches have developed. These are described in this publi cation with all the details required for practical application. Neither the editors nor NIST wish to endorse any single approach. Rather, we hope that their exposition will stimulate the dialogue which is a prerequisite for technical progress. Additionally, it is expected that those active in research in electron probe microanalysis will appreciate more clearly the areas in which further investigations are warranted.
Download or read book Ion Transport in Plants written by W. P. Anderson and published by Elsevier. This book was released on 2013-09-17 with total page 652 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ion Transport in Plants covers knowledge about ion transport in plants. The book discusses ultrastructural localization; formalism and membrane models; and membrane resistance and H+ fluxes. The text also describes ?+ fluxes in cells and organelles; Na+-?+ transport and ionic relations of the halophytes; and Cl- transport in vesicles. The ion transport in roots and the symplasm is also considered. Botanists, biochemists and biologists will find the book invaluable.
Download or read book Advanced Techniques in Biological Electron Microscopy written by J.K. Koehler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.
Download or read book Membrane Transport in Plants written by U. Zimmermann and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 475 pages. Available in PDF, EPUB and Kindle. Book excerpt: In February, 1974, an 'International Workshop on Membrane Transport in Plants' was held at the Nuclear Research Centre, JLiI ich, West Germany. More than two hundred and fifty people, from fourteen countries, took part in this highly successful meeting. A somewhat similar meeting took place in Liverpool, England, two years ago and it became clear there that progress in the field of membrane transport in plants was now so marked that a second, and wider, meeting in Germany was more than fully justified. The members of our pro gramme committee (U. Zimmermann, Chairman, JLilich (FRG); J. Dainty,
Download or read book Scanning Electron Probe Micro analysis with List of References written by and published by . This book was released on 1967 with total page 39 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Plant Electron Microscopy and Cytochemistry written by William V. Dashek and published by Springer Science & Business Media. This book was released on 2000-06-29 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on experimentalists describe the cutting-edge microscopical methods needed for the effective study of plant cell biology today. These powerful techniques, all described in great detail to ensure successful experimental results, range from light microscope cytochemistry, autoradiography, and immunocytochemistry, to recent developments in fluorescence, confocal, and dark-field microscopies. Important advances in both conventional and scanning electron microscopies are also fully developed, together with such state-of-the-art ancillary techniques as high-resolution autoradiography, immunoelectron microscopy, X-ray microanalysis, and electron systems imaging. Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.
Download or read book U S Environmental Protection Agency Library System Book Catalog Holdings as of July 1973 written by United States. Environmental Protection Agency. Library Systems Branch and published by . This book was released on 1974 with total page 702 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Transport in Plants III written by C.R. Stocking and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: The problems associated with the movement of water and solutes throughout the plant body have intrigued students of plants since Malpighi's conclusions in 1675 and 1679 that nutrient sap flows upward and downward in stems through vessels in both wood and bark. Steven Hale's ingenious experiments on the movement of water in plants in 1726 and Hartig's observations of sieve-tube exudation in the mid-19th century set the stage for continued intensive studies on long-range transport in plants. In spite of this interest for more than 200 years in the movement of solutes and water in plants, it has only been within the last 20 to 30 years that extensive research effort has been directed toward a critical evaluation of the interactions among the various cellular organelles. The important roles played by the exchange of metabolites in the control and regulation of cellular processes is now widely recognized, but in most instances poorly understood.
Download or read book Microwave Techniques and Protocols written by Richard T. Giberson and published by Springer Science & Business Media. This book was released on 2008-05-09 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: Richard Giberson and Richard Demaree, Jr, have collected a wide range of time-saving microwave techniques for processing biological samples for evaluation by many different microscopic methods. Described in step-by-step detail by hands-on researchers, these readily reproducible protocols include both optimized classic methods and such state-of-the art techniques as in vivo labeling, formalin fixation of fresh tissue, vacuum processing, and processing for scanning electron microscopy. Each stand-alone microwave method has been handcrafted by a researcher who regularly uses it to ensure processing success and the brightest quality result.
Download or read book Electron Probe Microanalysis written by A. J. Tousimis and published by Elsevier. This book was released on 2013-11-06 with total page 463 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Probe Microanalysis presents a collection of reviews on various aspects of electron probe microanalysis. This book discusses the model for quantitative electron probe analysis. Organized into 14 chapters, this book begins with an overview of the various kinds of microanalysis followed by a discussion of the advantages that can be derived from using the electron probe method. This text then examines the various applications of backscattered electron and specimen current methods for quantitative analysis. Other chapters consider the fundamental concepts for quantitative electron probe microanalysis utilizing pure elements as standards. This book discusses as well the absolute method of quantitative chemical analysis by emission X-ray spectroscopy. The final chapter deals with the main advantage of the Kossel technique in the study of the thermodynamic and mechanical characteristics of crystals. This book is a valuable resource for scientists and research workers. Non-specialists who need information on this excellent analytical tool will also find this book useful.
Download or read book Laboratory Procedures and Their Applications written by Indra Vasil and published by Elsevier. This book was released on 2012-12-02 with total page 860 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laboratory Procedures and Their Applications