Download or read book Methods of Protein and Nucleic Acid Research written by Lev Abramovich Osterman and published by Springer. This book was released on 1984 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods of Protein and Nucleic Acid Research written by L.A. Osterman and published by Springer. This book was released on 1984-02-01 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electrophoresis is the leading method among those used in the investigation of pro teins and nucleic acids. A paper on the study of these biopolymers without recourse to electrophoresis at each fractionation or characterization step is very unlikely to be encountered in the current scientific literature. This method enables separation of macromolecules according to characteristic features such as size (or molecular weight), shape, secondary structure and electric charge and these parameters can in fluence electrophoretic properties either separately or jointly. The physical background of this method is as follows. Macromolecules in a buf fer solution may become charged; the sign and magnitude of the total electrical charge depending on the pH of the medium. When such a solution is put into an insulated channel, e.g. in a glass tube, and a voltage applied at the ends of the chan nel, an electric field will be formed and direct current will pass through solution. The field strength can be defined as the potential difference at the ends of the chan nel (or its section) with respect to its length (v/cm). When exposed to an electrical field macromolecules will migrate towards the cathode or the anode according to their net charge and frictional force will limit the migration velocity.
Download or read book Methods of Protein and Nucleic Acid Research written by L.A. Osterman and published by Springer. This book was released on 1984-02-01 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Introduction to Biophysical Methods for Protein and Nucleic Acid Research written by Jay A. Glasel and published by Academic Press. This book was released on 1995-11-20 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first of its kind, Introduction to Biophysical Methods for Protein and Nucleic Acid Research serves as a text for the experienced researcher and student requiring an introduction to the field. Each chapter presents a description of the physical basis of the method, the type of information that may be obtained with the method, how data should be analyzed and interpreted and, where appropriate, practical tips about procedures and equipment.Key Features* Modern Use of Mass Spectroscopy* NMR Spectroscopy* Molecular Modeling and Graphics* Macintosh and DOS/Windows 3.x disks
Download or read book Enzymes Receptors and Carriers of Biological Membranes written by A. Azzi and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 169 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual follows at a distance of 3 years the previous one entitled Mem brane Proteins, and, like its predecessor, it is the result of an International Advanced Course sponsored by FEBS, SKMB and SNG, which was held in Bern in September 1983. The experiments offered to the students in the course had to be largely up· dated or chosen from new areas of membrane research, because of the sub stantial and rapid development of the field. Using the protocols of the course, the participants (graduate students, postdoctoral fellows and also senior scientists), in most cases not at all ex pert in biomembrane research, were able to repeat all the experiments suc cessfully. Those few protocols which for some reason did not fulfill the role we expected were modified. These protocols have now been collected in this manual, which we are able to offer to a number of biology, biochemistry and biophysics laborato ries, hoping that the selected number of methods which have been success fully used during the Advanced Course may be useful to them. This manual is also intented for teachers of practical classes, who may use it as a text book and as source of selected references, collected not in the library, but in the laboratory, from the notebooks of the young researchers who have contributed so much to the success of the Course.
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Download or read book Methods of Protein and Nucleic Acid Research written by Lev Abramovich Osterman and published by Springer. This book was released on 1984 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
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