Download or read book Methods in Ultrastructural Cytochemistry of the Cell Nucleus written by Gilles Moyne and published by . This book was released on 1980 with total page 98 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Interpretation of Ultrastructure written by R. J. C. Harris and published by Academic Press. This book was released on 2014-07-15 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Interpretation of Ulstrastructure is a collection of papers dealing with techniques in ulstrastructural research such as in fixation, reversed staining, ultrastructure in embryos and in plants, control of observations by light microscopy, and cytochemistry at the electron microscope level. One paper describes various fixatives used on rat liver tissue including a standard osmium tetroxide solution, pure melted osmium tetroxide, and strong osmium tetroxide solutions in carbon tetrachloride. Another paper points out that the use of electron micrographs of the cell nucleus and chromosomes should include approaches to chromosome analysis. For the investigator studying the three dimensional organization of chromosomes, his approaches should include classical cytology and cytogenetics, chemical and cytochemical studies on nuclei, as well as chemical and physical analysis of nucleic acid and nucleoprotein molecules. Several papers discuss the study of natural and artificial DNA-plasma by thin sections, an assessment of negative staining techniques for revealing ultrastructure, and other approaches in the study of biological ultrastructure by high-resolution electron microscopy. The collection will benefit microbiologists, biotechnologists, and academicians connected with the biological sciences.
Download or read book Pathology of the Nucleus written by James C.E. Underwood and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 491 pages. Available in PDF, EPUB and Kindle. Book excerpt: New techniques make it possible for investigators to examine and sometimes quantify various aspects of nuclear morphology and function; now they can derive clinically and biologically useful information about the nucleus. This book draws together a series of techniques which have been successfully applied to the study of the nucleus of tumour cells. These are of fundamental importance to researchers in tumour histopathology and medical oncology. Detailed reviews are given of various aspects of the morphology, ploidy and karyotypic status and function of the nuclei in the cells of tumours and preneoplastic lesions. Topics covered include nuclear morphology in tumour diagnosis, the ultrastructure of the nucleus, karyotypic analyses of solid tumours, flow cytometric assessment of nuclear ploidy and other parameters, histomorphometry of the nucleus, and in situ hybridisation.
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Introduction to Electron Microscopy for Biologists written by and published by Academic Press. This book was released on 2008-10-22 with total page 562 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers). Basic techniques in transmission and scanning electron microscopy Detailed chapters on how to use electron microscopy when dealing with specific cellular structures, such as the nucleus, cell membrane, and cytoskeleton Discussion on electron microscopy of viruses and virus-cell interactions
Download or read book Functional Ultrastructure written by Margit Pavelka and published by Springer Science & Business Media. This book was released on 2005-12-05 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: The period between 1950 and 1980 were the golden It also provides unique insights into how pathological years of transmission electron microscopy and pro- processes affect cell organization. duced a plethora of new information on the structure of This information is vital to current work in which cells that was coupled to and followed by biochemical the emphasis is on integrating approaches from p- and functional studies. TEM was king and each micro- teomics, molecular biology, molecular imaging and graph of a new object produced new information that physiology, and pathology to understand cell functions led to new insights on cell and tissue organization and and derangements in disease. In this current era, there is their functions. The quality of data represented by the a growing tendency to substitute modern light mic- images of cells and tissues had been perfected to a very scopic techniques for electron microscopy because it is high level by the great microscopists of the era including less technically demanding and is more readily available Palade, Porter, Fawcett, Sjostrand, Rhodin and many to researchers. This atlas reminds us that the infor- others. At present, the images that we see in leading tion obtained by electron microscopy is invaluable and journals for the most part do not reach the same techni- has no substitute.
Download or read book Ultrastructure Techniques for Microorganisms written by H.C. Aldrich and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: The modem microbiologist is often a real specialist who has difficulty under standing and applying many of the techniques beyond those in his or her own immediate field. On the other hand, most benefits to modem microbiology are obtained when a broad spectrum of scientific approaches can be focused on a problem. In early studies, electron microscopy was pivotal in understanding bacterial and viral morphology, and we still feel that we will understand a disease better if we have seen an electron micrograph of the causative agent. Today, because there is an increased awareness of the need to understand the rela tionships between microbial structure and function, the electron microscope is still one of the most important tools microbiologists can use for detailed analysis of microorganisms. Often, however, the aforementioned modem microbiologist still thinks of ultrastructure as involving negative staining or ultrathin sectioning in order to get a look at the shape of a "bug. " Many of the newer ultrastructure techniques, such as gold-labeled antibody localization, freeze-fracture, X-ray microanalysis, enzyme localization, and even scanning electron microscopy, are poorly under stood by, and therefore forbidding to, the average microbiologist. Even many cell biologists admit to having difficulty staying in touch with current develop ments in the fast-moving field of electron microscopy techniques.
Download or read book The Cell Nucleus V3 written by Harris Busch and published by Elsevier. This book was released on 2012-12-02 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Cell Nucleus, Volume III focuses mainly on nucleic acids, nuclear proteins, and special aspects of nuclear functions. This volume particularly discusses the organization of bacterial and viral DNA, as well as the nuclear DNA of eukaryotic organisms. It also describes nuclear DNA polymerases, precursors of messenger RNA, ribonucleoproteins, and nuclear high- and low-molecular-weight RNAs. Furthermore, this volume looks into the two broad classes of nuclear proteins: histones and nonhistone proteins. It also presents advances made in the knowledge of mammalian DNA-dependent RNA polymerase, cytochemical detection of nuclear enzymes, and nuclear protein synthesis. Moreover, it elucidates the effects of female steroid hormones on target cell nuclei, describes the nucleus during avian erythropoiesis, and reports the general properties of intranuclear viruses.
Download or read book Cytology and Cell Physiology written by Geoffrey Bourne and published by Elsevier. This book was released on 2012-12-02 with total page 799 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytology and Cell Physiology, Third Edition focuses on cell cytology and physiology as well as recent advances in the techniques in studying cells, including microscopy. It also describes cell membranes, surface, and physiology; cytoplasmic constituents; nucleus and nucleocytoplasmic reactions; enzyme histochemistry and cytochemistry; viruses within cells; and morphology of the cancer cell. Organized into 13 chapters, this edition begins with a historical overview of cytology, the template hypothesis of protein synthesis, and the respiratory function of mitochondria. It then discusses the subcellular components and their centrifugal isolation, some general principles of microscopy, selected physical and physicochemical methods, applications of enzyme histochemistry to electron microscopy, and structure and physical properties of the plasma membrane. The remaining chapters focus on the endoplasmic reticulum, the Golgi apparatus, the nucleus and its role in cell metabolism, RNA synthesis and movement, the behavior of viruses within cells, and pathological changes in cells. The book concludes with a chapter on the function and metabolism of cancer cells. This book is highly recommended to cytologists, investigators in the field of pathology, and graduate students in biology, biochemistry, physiology, and anatomy.
Download or read book The Nucleolus written by Mark O. J. Olson and published by Springer Science & Business Media. This book was released on 2011-09-15 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: Within the past two decades, extraordinary new functions for the nucleolus have begun to appear, giving the field a new vitality and generating renewed excitement and interest. These new discoveries include both newly-discovered functions and aspects of its conventional role. The Nucleolus is divided into three parts: nucleolar structure and organization, the role of the nucleolus in ribosome biogenesis, and novel functions of the nucleolus.
Download or read book Stains and Cytochemical Methods written by M.A. Hayat and published by Springer Science & Business Media. This book was released on 1993-03-31 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: The author's primary aim is to thoroughly explain the biochemical concepts governing cytochemical procedures for transmission electron microscopy. Such information provides undergraduate and graduate students, technicians, and researchers with a more profound understanding of electron micrographs, as well as the knowledge to refine existing techniques and develop new methodologies.
Download or read book Histochemical and Cytochemical Methods of Visualization written by Jean-Marie Exbrayat and published by CRC Press. This book was released on 2013-06-05 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: Histochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity. Histochemical and Cytochemical Methods of Visualizatio
Download or read book Immunocytochemistry of Plant Cells written by Kevin Vaughn and published by Springer Science & Business Media. This book was released on 2013-03-27 with total page 135 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunocytochemistry of plant cells is the first book exclusively dedicated to this topic. The first and largest portion of the book is concerned with a group of proven protocols and variations on these protocols that might prove useful, many developed or modified in the author's laboratory. The second portion of the book covers the studies that have been published previously on each of the plant organelles. Numerous state of the art micrographs from researchers around the world are included to demonstrate typical results.
Download or read book Methods in Plant Electron Microscopy and Cytochemistry written by William V. Dashek and published by Springer Science & Business Media. This book was released on 2000-06-29 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on experimentalists describe the cutting-edge microscopical methods needed for the effective study of plant cell biology today. These powerful techniques, all described in great detail to ensure successful experimental results, range from light microscope cytochemistry, autoradiography, and immunocytochemistry, to recent developments in fluorescence, confocal, and dark-field microscopies. Important advances in both conventional and scanning electron microscopies are also fully developed, together with such state-of-the-art ancillary techniques as high-resolution autoradiography, immunoelectron microscopy, X-ray microanalysis, and electron systems imaging. Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.
Download or read book Biological Specimen Preparation for Transmission Electron Microscopy written by Audrey M. Glauert and published by Princeton University Press. This book was released on 2014-07-14 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements. All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs. Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor Originally published in 1999. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.
Download or read book Nuclear Structure and Function written by I.B. Zbarsky and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 479 pages. Available in PDF, EPUB and Kindle. Book excerpt: This collection of 101 short communications, submitted by some of the participants at the 11th Nuclear Workshop held in Suzdal, USSR, 18-23 September, 1989, provides a representative survey of the material presented at the Workshop. Articles have been submitted by both those who delivered lectures and those who had poster presentations. The order of presentation at the Nuclear Workshop is roughly maintained within this proceedings book, but the session titles within the scientific program have not been utilized as discrete subdivisions within the book, because of the considerable overlap of subject matter. The overall sequence is as follows: Genome structure, Gene Structure and Expression, Nucleolar Genes, Structure and Proteins, Chromatin and Nuclear Granules, Nuclear Matrix and Nuclear Proteins, Replication and Transcription and finally Nuclear Envelope and Nuclear Cytoplasmic Transport. Several articles on Nuclear Lipids are also included, stemming from an evening round-table discussion on lipids. The third Wilhelm Bernhard Lecture was delivered in Suzdal by Professor Harris Busch, who can be seen in the photograph above (on the left) in the presence of Professor Ilya B. Zbarsky, President of the organizing committee for the 11th Nuclear Workshop. (Previous Wilhelm Bernhard lecturers have been Ronald H. Reeder, in Krakow, Poland, 1985 and Oscar L. Miller, Jr. , in Stevensbeek, The Netherlands, in 1987).
Download or read book Visualization of Nucleic Acids written by Gerard Morel and published by CRC Press. This book was released on 1995-03-30 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a review of the principle approaches for visualizing DNA and RNA. Using scanning tunneling and atomic force microscopes, the three-dimensional image of the surface of nucleic acids can be seen at atomic-scale resolutions. Spreading methods provide useful details on structural features of isolated molecules, but the major constituent of living matter is water, and the cryomicroscope makes it possible to look at DNA in its aqueous environment. Genes can be detected simultaneously in situ in chromosomes using fluorescent probes, and also at the electron microscopic level. In cells, nucleic acids are localized and quantified by dyes; electron microscopy is used with cytochemical, immunocytological, nuclease, and in situ hybridization methods. The main potential applications for pathological studies are shown with particular aspects such as viral nucleic acids and in situ PCR.
