Download or read book Methods in Molecular Biology YAC protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book YAC Protocols written by Alasdair MacKenzie and published by Springer Science & Business Media. This book was released on 2007-10-26 with total page 209 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is now 10 years since the first edition of YAC Protocols was published in 1996. YAC Protocols was first produced to address the huge demand within the research community for a lab-based text that described in detail the wide range of uses for large insert yeast artificial chromosome (YAC) DNA clones. In doing this, the original editor, David Markie, and the many different contri- tors who provided descriptions of the protocols they used and developed, did a magnificent job. Indeed many of the techniques described within the first e- tion require little change and have stood up admirably to the test of time. Since the first edition, the use of YACs has proved invaluable for addressing a wide range of new biological problems ranging from those of basic biochemistry to assisting in the mapping and sequencing of the human genome. The requirement for a second edition of YAC Protocols was prompted by a number of major advances in biology since the publication of the first edition. These advances have included the sequencing of the human genome, and the genomes of a wide variety of other organisms, and the increased use of transgenic animals for understanding the molecular basis of human and animal disease.
Download or read book YAC Protocols written by Alasdair MacKenzie and published by Humana. This book was released on 2006-08-18 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is now 10 years since the first edition of YAC Protocols was published in 1996. YAC Protocols was first produced to address the huge demand within the research community for a lab-based text that described in detail the wide range of uses for large insert yeast artificial chromosome (YAC) DNA clones. In doing this, the original editor, David Markie, and the many different contri- tors who provided descriptions of the protocols they used and developed, did a magnificent job. Indeed many of the techniques described within the first e- tion require little change and have stood up admirably to the test of time. Since the first edition, the use of YACs has proved invaluable for addressing a wide range of new biological problems ranging from those of basic biochemistry to assisting in the mapping and sequencing of the human genome. The requirement for a second edition of YAC Protocols was prompted by a number of major advances in biology since the publication of the first edition. These advances have included the sequencing of the human genome, and the genomes of a wide variety of other organisms, and the increased use of transgenic animals for understanding the molecular basis of human and animal disease.
Download or read book The Nucleic Acid Protocols Handbook written by Ralph Rapley and published by Springer Science & Business Media. This book was released on 2008-06-29 with total page 997 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."
Download or read book Short Protocols in Molecular Biology written by Frederick M. Ausubel and published by Current Protocols. This book was released on 1999-05-03 with total page 1134 pages. Available in PDF, EPUB and Kindle. Book excerpt: Short Protocols in Molecular Biology Fourth Edition The Desktop Guide to Your Lab Edited by Frederick M. Ausubel, Roger Brent, Robert E. Kingston, David D. Moore, J. G. Seidman, John A. Smith, and Kevin Struhl Providing condensed descriptions of more than 600 methods compiled from Current Protocols in Molecular Biology, this updated edition of the classic laboratory manual thoroughly explores molecular biology in an easily accessible, hands-on format. Examining the physiochemical organization of living matter from a molecular basis requires a text which is informative and well annotated-Short Protocols in Molecular Biology, Fourth Edition offers both. The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research. The authors have enriched the text with diagrams, charts, and material lists to enhance comprehension of the material and facilitate the experimental set-up. This edition has been expanded to include the latest developments in cutting-edge techniques such as fluorescent DNA sequencing, PCR optimization, yeast two-hybrid/interaction trap analysis, and sequence similarity searching using Blast. Classic techniques in plasmid and phage manipulation and mammalian cell selection have also benefited from the updating and reflect the methods currently used in leading research facilities around the world. New topics to this edition include: * Informatics for Molecular Biologists * Analysis of Protein Interactions * Epitope Tagging * Mathematics and Statistics for Molecular Biologists Short Protocols in Molecular Biology, Fourth Edition is an authoritative and indispensable guide for all life scientists and researchers who are looking to improve their understanding of molecular biology methods.
Download or read book Molecular Biology and Biotechnology written by M. P. Bansal and published by The Energy and Resources Institute (TERI). This book was released on 2013-01-01 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Biology and Biotechnology: basic experimental protocols is a compilation of methods and techniques commonly used in biomedical and biotechnological studies. The book aims to provide ample support to both students and faculty while conducting practical lessons. Four sections are covered in this book—Genomics, Proteomics, Quantitative Biochemistry, and Bioinformatics. A concise introductory note accompanies each protocol/method described for better comprehension. Every topic discussed is supported by actual methods and their expected results, and is accompanied by relevant questions.
Download or read book Short Protocols in Molecular Biology written by Frederick M. Ausubel and published by . This book was released on 2002 with total page 838 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recently expanded to 2 volumes, Short Protocols in Molecular Biology, Fifth Edition, provides condensed descriptions of more than 700 methods compiled from Current Protocols in Molecular Biology. Includes new chapters on chromatin assembly and analysis, nucleic acid arrays, generation and use of combinatorial libraries, discovery and analysis of differentially expressed genes in single cells and cell populations. The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research Short Protocols in Molecular Biology, Fifth Edition is an authoritative and indispensable guide for all life scientists, researchers, and students at the graduate and advanced undergraduate level Expanded to 2 volumes.
Download or read book The Condensed Protocols from Molecular Cloning written by Joseph Sambrook and published by CSHL Press. This book was released on 2006 with total page 802 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Condensed Protocols From Molecular Cloning: A Laboratory Manualis a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual.This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world's best–selling manual of molecular biology techniques. Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning.
Download or read book Protocols in Human Molecular Genetics written by Christopher G. Mathew and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 461 pages. Available in PDF, EPUB and Kindle. Book excerpt: Extraordinary advances have been made in the field of human molecular genetics during the past five years. The ability to amplify a specific region ofDNA a millionfold in a few hours using the polymerase chain reaction has led to the rapid identification of mutations in human disease and of DNA sequence polymorphisms on every human chro- some. DNA fragments of up to 1 megabase in length can now be resolved by pulsed-field gel electrophoresis to create long-range physical maps of important regions of the genome, and can be cloned in the form of yeast artificial chromosomes. The discovery of highly variable "minisatellite" DNA sequences has led to the development of DNA fingerprinting. The application of these techniques to the study of the human genome has culminated in major advances such as the cloning of the cystic fibrosis gene, the construction of genetic linkage maps of each human chro- some, the mapping of many genes responsible for human inherited d- orders, genetic fingerprinting of forensic specimens, and the detection of mutations involved in the development of human tumors. Although many of the new techniques in molecular genetics can be learned relatively easily, it is often difficult for a researcher to obtain all of the relevant information necessary for getting up a technique and applying it successfully. The information available in the research lite- ture often lacks the depth of description that the new user requires.
Download or read book Molecular Biomethods Handbook written by Ralph Rapley and published by Springer. This book was released on 2007-10-09 with total page 708 pages. Available in PDF, EPUB and Kindle. Book excerpt: An authoritative team of investigators illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced scientists fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included. The goal is to provide established scientists and novices who are new to these techniques with a deeper understanding of the widest variety of biotechniques and their applications.
Download or read book Methods in Molecular Biology Yeast protocols methods in cell and molecular biology written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genomic Imprinting written by Andrew Ward and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genomic imprinting is the process by which gene activity is regulated according to parent of origin. Usually, this means that either the maternally inherited or the paternally inherited allele of a gene is expressed while the opposite allele is repressed. The phenomenon is largely restricted to mammals and flowering plants and was first recognized at the level of whole genomes. Nuclear transplantation experiments carried out in mice in the late 1970s established the non-equivalence of the maternal and paternal genomes in mammals, and a similar conclusion was drawn from studies of interploidy crosses of flowering plants that extend back to at least the 1930s. Further mouse genetic studies, involving animals carrying balanced translocations (reviewed in Chapter 3), indicated that imprinted genes were likely to be widely scattered and would form a minority within the mammalian genome. The first imprinted genes were identified in the early 1990s; over forty are now known in mammals and the list continues steadily to expand.
Download or read book Current Protocols in Molecular Biology written by Frederick M. Ausubel and published by . This book was released on 1987 with total page 1020 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mammalian Artificial Chromosomes written by Vittorio Sgaramella and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 1996, we organized a workshop, inter alia, at the National Research Co- cil in Milan under the generous sponsorship of the European Science Foun- tion. On that occasion, a small group of investigators convened from many countries and presented early evidence of the possibility of assembling basic units of mammalian chromosomes into artificial constructs (or, indeed, red- ing the relevant components to more manageable dimensions and defined c- stitution). Progress in the following years has been slow but steady. Many scientists who took part in the workshop have since been engaged in active and prod- tive research. It goes to the credit of Humana Press to have realized the need for a book on artificial chromosomes that aims to provide better tools to all scientists committed to this field who are confronted with very difficult tech- cal problems. We have strived to cover in Mammalian Artificial Chromosomes: Methods and Protocols all relevant areas of artificial chromosome research, from basic genetics to daring attempts to build new tools for genetic therapy. We are of course grateful to the authors who have accepted the task of describing the technical steps and pitfalls that can be encountered in their research. Rarely has a very delicate methodology been presented with such meticulous care. We have been helped in this enterprise by the excellent librarian of the LITA Institute in Segrate, Italy, Ms. Claudia Piergigli, whom we thank warmly. Ms.
Download or read book Protocols for Gene Analysis written by Adrian J. Harwood and published by Humana. This book was released on 1994-04-27 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is now twenty years since Cohen and Boyer's first steps into DNA cloning. In the time since then, there has been an ever increasing acc- eration in the development and application of the cloning methodology. With the recent development of the polymerase chain reaction, a second generation of the technology has been born, enabling the isolation of DNA (and in particular, genes) with little more information than the p- tial knowledge of the sequence. In fact, DNA sequencing is now so advanced that it can almost be carried out on the industrial scale. As a consequence of these advances, it now appears feasible to sequence whole genomes, including one the size of the human. What are we going to do with this information? The future of basic molecular biology must lie in the ability to analyze DNA (and especially the genes within it) starting at the DNA level. It is for these problems that Protocols for Gene Analysis attempts to offer solutions. So you have a piece of DNA, possibly a gene--what do you do next? The first section of this book contains a number of "basic" te- niques that are required for further manipulation of the DNA. This s- tion is not intended to be a comprehensive collection of methods, but merely to serve as an up-to-date set of techniques. I refer you to other volumes in the Methods Molecular Biology series for further rec- binant DNA techniques.
Download or read book Methods in Molecular Biology PCR cloning protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Molecular Biology Basic DNA and RNA protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
