Download or read book Methods for Imaging Cell Membranes written by Luca Panconi and published by CRC Press. This book was released on 2023-12-11 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will serve as an introduction to microscopy and biomedical imaging methods, with a focus on the study of the distributions and dynamics of molecules on the cell surface. It will provide readers with an in-depth understanding of how modern microscopy methodology can be used to understand the organisation of cell membrane systems and how experiments can be designed around these methodologies. There are numerous methods employed to understand cell membrane organisation, but foremost among them are microscopy methods which can map the distributions of molecules on the cell surface and even map the biophysical properties of membranes themselves. Fluorescence microscopy has been especially widely used due to its specificity and relatively noninvasive nature, allowing live-cell imaging. However, the recent advance of super-resolution fluorescence microscopy has broken the previous resolution limit for this type of microscopy, which has been an important advancement in the field. Atomic force microscopy and electron microscopy have also been deployed to learn about membrane organisation and properties. Each chapter in this volume will be themed around measuring a particular property of cell membranes. In each case, the authors examine the range of methodology applicable to the task, comparing the advantages and disadvantages of each one, and will also provide an overview of important discoveries that have been made using the methodology being discussed. The chapters will cover: • Measuring membrane protein distributions using single-molecule localisation microscopy (SMLM) • Measuring membrane protein dynamics and diffusion using fluorescence correla-tion spectroscopy (FCS) • Mapping membrane lipid backing using environmentally sensitive fluorescence probes • Mapping membrane thickness and rigidity using atomic force microscopy • Mapping membrane proteins and the cytoskeleton using electron microscopy This book will be a valuable resource to graduate and upper-level undergraduate students and industry researchers in the fields of cell biology, microbiology, microscopy, and medical imaging.

Download or read book Methods for Imaging Cell Membranes written by Luca Panconi and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will serve as an introduction to microscopy and biomedical imaging methods, with a focus on the study of the distributions and dynamics of molecules on the cell surface. It will provide readers with an in-depth understanding of how modern microscopy methodology can be used to understand the organisation of cell membrane systems and how experiments can be designed around these methodologies. There are numerous methods employed to understand cell membrane organisation, but foremost among them are microscopy methods which can map the distributions of molecules on the cell surface and even map the biophysical properties of membranes themselves. Fluorescence microscopy has been especially widely used due to its specificity and relatively noninvasive nature, allowing live-cell imaging. However, the recent advance of super-resolution fluorescence microscopy has broken the previous resolution limit for this type of microscopy, which has been an important advancement in the field. Atomic force microscopy and electron microscopy have also been deployed to learn about membrane organisation and properties. Each chapter in this volume will be themed around measuring a particular property of cell membranes. In each case, the authors examine the range of methodology applicable to the task, comparing the advantages and disadvantages of each one, and will also provide an overview of important discoveries that have been made using the methodology being discussed. The chapters will cover: •Measuring membrane protein distributions using single-molecule localisation microscopy (SMLM) •Measuring membrane protein dynamics and diffusion using fluorescence correla-tion spectroscopy (FCS) •Mapping membrane lipid backing using environmentally sensitive fluorescence probes •Mapping membrane thickness and rigidity using atomic force microscopy •Mapping membrane proteins and the cytoskeleton using electron microscopy This book will be a valuable resource to graduate and upper-level undergraduate students and industry researchers in the fields of cell biology, microbiology, microscopy, and medical imaging.

Download or read book Cell Imaging Techniques written by Douglas J. Taatjes and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.

Download or read book New Methods and Sensors for Membrane and Cell Volume Research written by and published by Academic Press. This book was released on 2021-12-01 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: New Methods and Sensors for Membrane and Cell Volume Research, Volume 88 provides an overview of novel experimental approaches to study both the cell membrane and the under-membrane space – the cytosol, which have lately began drawing renewed attention. The book's overall emphasis is on fluorescent and FRET-based sensors, however, other optical (such as variants of transmission microscopy) and non-optical methods (neutron scattering and mass spectrometry) also have dedicated chapters. This volume provides a rare review of experimental approaches to study intracellular phase transitions, as well as anion channels, membrane tension and dynamics, and other topics of intense current interest. Describes novel FRET-based membrane sensors Reviews selected non-optical approaches to membrane structure and dynamics Describes traditional and modern aspects of cell volume research, such as phase transitions and macromolecular crowding

Download or read book Fluorescent Methods to Study Biological Membranes written by Yves Mely and published by Springer Science & Business Media. This book was released on 2012-10-10 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.

Download or read book Methods in Membrane Biology written by Edward D. Korn and published by Springer Science & Business Media. This book was released on 2013-11-21 with total page 283 pages. Available in PDF, EPUB and Kindle. Book excerpt: The contributions of electron microscopy to membrane biology have been indispensable and, at the same time, disappointing. Membranes were known to exist before the advent of electron microscopy and general principles of their composition and molecular organization had been deduced from permeability and electrical conductivity measurements, polarized light microscopy, and X-ray diffraction. On the other hand, the complexities of the many intracellular membranes and membranous organelles were really not suspected until they were observed by the electron microscopist. One then had further hopes that the high resolution of the electron microscope (theoretically it can resolve atomic distances) would allow the visualization of the molecular architecture of membranes and lead directly to an under standing of structure and function at the molecular level. This expectation has been largely unrealized. Because of the great difficulties encountered in the preparation of biological material, because of the uncertainty of the chemistry of "staining," and because of numerous electron optical artifacts, it has been a major challenge just to rationalize the observed images in terms of the known facts, let alone to utilize the images to expand our knowledge of the molecular structure of biological membranes. The many differences among membranes with respect to function and composition are lost in the universal trilamellar image. Perhaps the one major exception to this, and the major structural contribution of electron microscopy at the molecular level, has come from freeze-etch electron microscopy.

Download or read book Methods in Cellular Imaging written by Ammasi Periasamy and published by Springer. This book was released on 2013-05-27 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided.

Download or read book Cell Membrane Nanodomains written by Alessandra Cambi and published by CRC Press. This book was released on 2014-10-27 with total page 514 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Membrane Nanodomains: From Biochemistry to Nanoscopy describes recent advances in our understanding of membrane organization, with a particular focus on the cutting-edge imaging techniques that are making these new discoveries possible. With contributions from pioneers in the field, the book explores areas where the application of these novel techniques reveals new concepts in biology. It assembles a collection of works where the integration of membrane biology and microscopy emphasizes the interdisciplinary nature of this exciting field. Beginning with a broad description of membrane organization, including seminal work on lipid partitioning in model systems and the roles of proteins in membrane organization, the book examines how lipids and membrane compartmentalization can regulate protein function and signal transduction. It then focuses on recent advances in imaging techniques and tools that foster further advances in our understanding of signaling nanoplatforms. The coverage includes several diffraction-limited imaging techniques that allow for measurements of protein distribution/clustering and membrane curvature in living cells, new fluorescent proteins, novel Laurdan analyses, and the toolbox of labeling possibilities with organic dyes. Since superresolution optical techniques have been crucial to advancing our understanding of cellular structure and protein behavior, the book concludes with a discussion of technologies that are enabling the visualization of lipids, proteins, and other molecular components at unprecedented spatiotemporal resolution. It also explains the ins and outs of the rapidly developing high- or superresolution microscopy field, including new methods and data analysis tools that exclusively pertain to these techniques. This integration of membrane biology and advanced imaging techniques emphasizes the interdisciplinary nature of this exciting field. The array of contributions from leading world experts makes this book a valuable tool for the visualization of signaling nanoplatforms by means of cutting-edge optical microscopy tools.

Download or read book Methods in Membrane Biology written by Edward Korn and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many of the methods now in general use in membrane biology, and not already discussed in satisfactory detail elsewhere, have been covered in the eight previously published volumes of this series. Much of this ninth volume is occupied by one authoritative chapter, an unusually thorough and critical review of a relatively new and highly specialized technology that has gained rapid acceptance: immunofluorescence and immunoelectron microscopy. These are powerful experimental tools applicable in fields much broader than membrane research and employing methods drawn from widely diverse disciplines such as organic chemistry, protein chemistry, immunology, and fluorescence and electron microscopy. The temptation to use these super ficially, and deceptively, simple but fundamentally complex methods un critically is almost overwhelming. The chapter by de Petris, a pioneer in the field, is as necessary as it is rigorous, and it should long be the standard in this area of research. The second chapter in this volume is a more specialized review by Matus of the procedures for the preparation and characterization of the highly differentiated junctional regions of brain plasma membranes. These methods are central to the rapidly growing field of neurobiochemistry membrane biochemistry at perhaps its most intricate.

Download or read book Cell Imaging written by David Stephens and published by Scion Publishing Ltd. This book was released on 2005-12-01 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the key techniques that can be employed in any lab with access to cell imaging equipment, even if they do not currently specialize in imaging. It focuses on live cell imaging and light microscopy applications, but is equally relevant to the imaging of fixed specimens.

Download or read book Methods in Membrane Biology written by Edward D. Korn and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 3 continues the approach carried out in the first two volumes of this se ries of publishing articles on membrane methodology which include, in addition to procedural details, incisive discussions of the ap plications of the methods and of their limitations. Wh at is the theoretical basis of the method, how and to what problems can it be applied, how does one interpret the results, what has thus far been achieved by the method, what lies in the future-these are the questions the authors have tried to answer. No area of membrane biology engages the interest of more investigators than studies of the plasma membrane. Four chapters in this volume are concerned with one or more aspects of the cell surface. Fundamental to all studies of the cell surface are the isolation and characterization of pure plasma membranes. Many preparations described in the literature are inadequate or are inadequately characterized. In the first chapter, Neville discusses the theoretical and practical bases of tissue fractionation, empha sizes the variations in enzyme content among plasma membranes from different sources, offers guidance in the choice of the proper criteria for assessing membrane purity, and suggests the best markers for detecting the possible presence of contaminating organelles. To review in detail each of the many preparations of plasma membranes that have been published is impossible.

Download or read book Live Cell Imaging written by Sung-Bae Kim and published by Humana. This book was released on 2022-06-12 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book highlights recent advances in molecular imaging techniques and protocols, designed to be immediately applicable in global bio-laboratories. The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent probes, functional substrates and luciferases, organic fluorescent probes, BRET probes, FRET probes, as well as with advanced instrumentation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Live Cell Imaging: Methods and Protocols aims to direct and inspire researchers into creating smarter, next-generation imaging techniques that are truly quantitative, highly sensitive, and readily comprehended, in the effort to engender deeper understanding of biological systems and break new ground in the research fields of life science.

Download or read book Membrane Potential Imaging in the Nervous System written by Marco Canepari and published by Springer Science & Business Media. This book was released on 2010-10-19 with total page 171 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book is structured in five sections, each containing several chapters written by experts and major contributors to particular topics. The volume starts with a historical perspective and fundamental principles of membrane potential imaging and continues to cover the measurement of membrane potential signals from dendrites and axons of individual neurons, measurements of the activity of many neurons with single cell resolution, monitoring of population signals from the nervous system, and concludes with the overview of new approaches to voltage-imaging. The book is targeted at all scientists interested in this mature but also rapidly expanding imaging approach.

Download or read book Cell Membranes written by E. Elson and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume assembles reviews on topics in two major related areas. One of these concerns the interactions of cells with substrata and with other cells, which are mediated by the extracellular matrix and soluble molecules. As described in this volume, these interactions are responsible for controlling cell functions ranging from embryogenesis and neural development to blood clotting. More over, important properties of the extracellular matrix can be modulated by the interdependent actions of tumor cells and fibroblasts. The other major area of interest concerns the response of cells to extracellular signals. Recent work has begun to reveal how a remarkable diversity of cellular functions, including neuronal, proliferative, membrane--cytoskeletal, and many other kinds of re sponses, are elicited through the mediation of a relatively small and interdepen dent set of second messenger systems. These include both changes in cytoplasmic ionic balances and activation of various kinds of protein kinases. Both subjects are covered in this volume. The two areas are linked by the common theme of cellular response to an external environment that is sensed through cellular interactions with informational molecules, which are soluble agents, as well as those that are components of insoluble matrices. It is only recently that we have come to appreciate the complex interplay between the matrix surrounding a cell and the cell's response to hormones and growth factors. Thus, we have tried to select examples in which this type of extracellular integration may playa role.

Download or read book Cellular Imaging Techniques for Neuroscience and Beyond written by Floris G. Wouterlood and published by Academic Press. This book was released on 2012-12-06 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: The imaging of small cellular components requires powerful instruments, and an entire family of equipment and techniques based on the confocal principle has been developed over the past 30 years. Such methods are commonly used by neuroscience researchers, but the majority of these users do not have a microscopy or a cell biology backgrounds and do can encounter difficulties in obtaining and interpreting results. This volume brings experts in high-resolution optical microscopy applications in neuroscience and cell biology together to document the state of the art. Outlining what is currently possible, the volume also discusses promising developments for the future and aids readers in selecting the most scientifically meaningful approach to solve their questions. Each chapter discusses instrumentation and technology in relationship to application in research. All of the common and cutting edge trends are covered - fluorescence / laser electron / nonlinear microscopy, infrared fluorescence, multiphoton imaging, tomography, FRAP, live imaging, STED, PALM/STORM, etc. Single and multiphoton confocal microscopy, and 4-pi confocal microscopy Obtaining nanoresolution via photoactivation localization microscopy (PALM) Several procedures that correlate observations in optical fluorescence microscopy and electron microscopy Study of morphology and function via high-resolution fluorescence procedures Additional high-resolution microscopic techniques

Download or read book Imaging Living Cells written by Rosario Rizzuto and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: Intended for bench-top use, this lab manual is suitable for both scientists and graduate students, since it combines an update on the most advanced imaging procedures with detailed protocols. Examples, carefully selected from the wide repertoire of cell pyhsiology, cover such different functional aspects as distribution of multiple ions, electrical activity, exo-endocytosis, gene expression, and the cell cycle.

Download or read book Cell Membranes written by Elliot Elson and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: We are extremely pleased that all of the chapters in this volume provide up-to-date information on a variety of topics of interest to scientists working on membrane biology. As in the past, we have attempted to expedite the transition from submission of the manuscripts to publication in order to make the reviews as timely as possible. Cell biology and molecular biology are increasingly be coming concerned with the study of structural elements in cells and their assem bly. The rules which govern membrane synthesis, assembly and interaction of membrane components with other cellular elements, notably the cytoskeleton, are at the center of research in these fields. We will continue in subsequent volumes of this series to focus on these areas. We would welcome suggestions of topics which would benefit from a review at the present time. We thank all of the contributors for providing these very excellent reviews and for doing so in a timely fashion. Elliot L. Elson William A. Frazier Luis Glaser St. Louis, Missouri vii CONTENTS Chapter 1 Chemotactic Transduction in the Cellular Slime Molds William A. Frazier, Beth L. Meyers-Hutchins, Gordon A. Jamieson, Jr. , and Nancy J. Galvin 1. Introduction-Chemotaxis in the Cellular Slime Molds . . . . . . . . . . . 2. Receptors for Chemoattractants of the Cellular Slime Molds . . . . . . . 4 2. 1. The Folate Receptor of Vegetative D. discoideum . . . . . . . . . . . 4 2. 2. The cAMP Receptor of Aggregating D. discoideum . . . . . . . . . 6 3. Putative Transduction Events in D. discoideum . . . . . . . . . . . . . . . . . 10 3. 1. cGMP and Guanylate Cyclase . . . . . . . . . . . . . . . . . . . . . . . . . . .