Download or read book Membrane Fusion Studies with Solid State NMR written by Prashant Radheshyam Agrawal and published by . This book was released on 2007 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Solid State NMR Studies of Structure and Dynamics of Membrane Associated Influenza Fusion Peptide written by Ujjayini Ghosh and published by . This book was released on 2016 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Solid state Nuclear Magnetic Resonance Structural Studies of the HIV 1 Fusion Peptide in the Membrane Environment written by Jun Yang and published by . This book was released on 2003 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Solid state Nuclear Magnetic Resonance Studies of the Structure and Membrane Insertion of HIV Fusion Peptides written by Wei Qiang and published by . This book was released on 2009 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Solid state NMR written by Frances Separovic and published by . This book was released on 2020 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this book is to describe the methodology and applications of solid-state NMR spectroscopy to studies of membrane proteins, membrane-active peptides and model biological membranes. As well as structural studies, this book contains coverage of membrane interactions and molecular motions. Advances in biological solid-state NMR are very pertinent with high-field developments seeing applications in biological membranes and whole cells. Experts who are leaders in the development and application of biological solid-state NMR are chapter contributors. Part of Biophysical Society-IOP series.
Download or read book Structure and Dynamics of Membrane Proteins from Solid state NMR written by Myungwoon Lee and published by . This book was released on 2018 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: Solid-state nuclear magnetic resonance (SSNMR) spectroscopy is an essential tool to elucidate the structure, dynamics, and function of biomolecules. This thesis mainly focuses on the structure determination of the hydrophobic domains for fusion proteins which are involved in membrane fusion between the cell membrane and viral envelope. Although extensive structural studies have been conducted on the soluble ectodomain by crystallography, the structural topologies of the hydrophobic TMD of the fusion proteins have been poorly understood. Here, we introduced SSNMR to investigate the secondary structure and oligomeric states of the TMD of two fusion proteins, PIV5 F and HIV gp41. For the PIV5 TMD, the membrane dependent secondary structure was determined by measuring the chemical shifts: predominant a-helical conformation in the POPC/cholesterol membrane shifts to the [Beta]-strand in the POPE membrane. Using 19F spin diffusion experiments on the fluorinated TMD, we have determined that the TMD forms a trimeric helical bundle. For the HIV gp4l MPER-TMD, we found the presence of a turn between the MPER helix and the TMD helix by measuring intramolecular distances and probing the lipid-peptide and water-peptide interactions. Intermolecular 19F- 19F distances of the fluorinated peptides indicate that the MPER-TMD is a trimeric. In addition to membrane fusion proteins, we have studied the oligomeric structure and the zinc-bound coordination geometry of a de novo designed amyloid fibril that catalyzes ester hydrolysis. By measuring the intermolecular contacts, we determined that peptides form parallel-in- register P-sheets and further assemble into stacked bilayers in an antiparallel orientation. The zinc binding sites were confirmed by the chemical shifts perturbation of histidines with zinc and the specific zinc-bound geometry was identified by measuring intra-residue distances of histidines. We also investigated the effects of cryoprotectants on the spectral resolution of lipid membranes and membrane peptides at low temperature. 13C and 1H MAS spectra of various cryoprotected membranes showed that DMSO provides the best resolution enhancement with the best ice formation retardation at low temperature and DLPE lipid exhibits the excellent resolution.
Download or read book Solid State Nuclear Magnetic Resonance Studies of the Influenza Fusion Peptide Associated with Membrane Bilayers written by Paul Parkanzky and published by . This book was released on 2006 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Perspectives on Solid State NMR in Biology written by S.R. Kiihne and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: Solid state NMR is rapidly emerging as a universally applicable method for the characterization of ordered structures that cannot be studied with solution methods or diffraction techniques. This proceedings -; from a recent international workshop - captures an image of the latest developments and future directions for solid state NMR in biological research, particularly on membrane proteins. Detailed information on how hormones or drugs bind to their membrane receptor targets is needed, e.g. for rational drug design. Higher fields are bringing clear improvements, and the power of solid state NMR techniques for studying amorphous and membrane associated peptides, proteins and complexes is shown by examples of applications at ultra-high fields. Progress in protein expression, experimental design and data analysis are also presented by leaders in these research areas.
Download or read book Coordination Among Proteins Lipids and Water in Membrane Fusion and Fission Probed by Solid state NMR written by Madeleine Sutherland and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: For enveloped viruses such as HIV, influenza, and coronaviruses to enter host cells, the viral and cell envelopes must be fused together. Then, to exit the cell, a new virus particle will pinch off a piece of the host cell membrane. The two membranes must be physically separated to release the virus particle. This process is called "scission" in the context of viral exit and "fission" when simply discussing membrane division." Viral membrane remodeling proteins catalyze membrane fusion and fission to bring about viral entry and exit from host cells -- vital processes in the life cycle of enveloped viruses. The exact mechanism by which membrane remodeling proteins catalyze membrane fusion and fission is not yet fully understood. The membrane remodeling proteins may take any combination of the following actions to facilitate fusion and fission: 1. Directly altering the local curvature of the membrane 2. Altering the line tension at L[subscript o]/L[subscript d] phase boundaries 3. Forming protein clusters to act collectively on the membrane 4. Altering the local composition of the membrane at the site of fusion or fission 5. Physically disrupting the lipid assemblies, i.e. by inserting protein domains into the membrane and creating membrane defects In this work, we examine the extent to which HIV's membrane fusion protein gp41, and Influenza A's membrane fission protein M2, utilize these five processes to carry out their functions. We use solid-state NMR techniques to probe intermolecular interactions along those lines, with an emphasis on curvature and clustering. The contributions of both proteins and lipids to these processes will be examined. The goal is to better understand the mechanisms of HIV entry and influenza release, which can hopefully guide the development of better therapeutics and vaccines.
Download or read book Characterization of Structure and Dynamics of Membrane Proteins from Solid state NMR written by Byungsu Kwon and published by . This book was released on 2018 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt: Solid-state nuclear magnetic resonance (ssNMR) spectroscopy is an essential tool for elucidating the structure, dynamics, and function of biomolecules. ssNMR is capable of studying membrane proteins in near-native lipid bilayers and is thus preferred over other biophysical techniques for characterizing the structure and dynamics of membrane proteins. This thesis primarily focuses on the study of the following membrane proteins: 1) the N-terminal ectodomain and C-terminal cytoplasmic domain of influenza A virus M2 and 2) HIV-1 glycoprotein gp4l membrane-proximal external region and transmembrane domain (MPER-TMD) in a near native membrane environment. The cytoplasmic domain of M2 is necessary for membrane scission and virus shedding. The M2(22-71) construct shows random-coil chemical shifts, large motional amplitudes, and a membrane surface-bound location with close proximity to water, indicating the post-amphipathic helix (AH) cytoplasmic domain is a dynamic random coil near the membrane surface. The influenza M2 ectodomain contains highly conserved epitopes but its structure is largely unknown. The M2(1-49) construct containing both the ectodomain and transmembrane domain exhibits an entirely unstructured ectodomain with a motional gradient in which the motion is slower for residues near the TM domain, which attributed to the formation of a tighter helical bundle in the presence of drug that should cause the more tightened C-terminal ectodomain, thereby slowing its local motions. HIV-1 virus gp4l is directly involved in virus-cell membrane fusion. However, the structural topologies of the gp4l MPER-TMD are still controversial and the biologically-relevant intrinsic conformational state of MPER has not yet been determined. In order to obtain near native structural information of gp4l, we have studied gp41 (665-704) and found a primarily a-helical conformation, membrane-anchored trimeric TMD and water-exposed membrane surface-bound MPER. Intra- and intermolecular distances measured using 19C-19F REDOR and 19F-19F CODEX revealed that MPER-TMD has a significant kink between MPER and TMD, which has aided a deeper understanding of the HIV virus entry mechanism and the design of vaccines.
Download or read book Advances in Biological Solid State NMR written by Frances Separovic and published by Royal Society of Chemistry. This book was released on 2014 with total page 632 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Biological NMR brings the reader up to date with chapters from international leaders of this growing field, covering the most recent developments in the methodology and applications of solid state NMR to studies of membrane interactions and molecular motions
Download or read book Structural Studies of Membrane associated Complexes by Solid state NMR written by and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Phospholipids Handbook written by Gregor Cevc and published by CRC Press. This book was released on 1993-08-02 with total page 1162 pages. Available in PDF, EPUB and Kindle. Book excerpt: Employing a multidisciplinary approach to phospholipid research, this work catalogues the current knowledge of this class of molecules and details the general, chemical, physical and structural properties of phospholipid monolayers and bilayers. Phospholipid applications are also covered.
Download or read book Multidimensional Solid State NMR Studies of Membrane Proteins written by and published by . This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Solid state NMR Studies of Phospholipid Model Membranes and Membrane associated Macromolecules written by Jun-Xia Lu and published by . This book was released on 2007 with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt: The effect of cholesterol on the bicelles was further studied utilizing both solid-state 2H NMR and the EPR spin labeling approach. The results indicate a higher molecular ordering of the lipids and a higher alignment transition temperature of the bicelles in the presence of cholesterol.
Download or read book High Resolution Structural Studies of Membrane Proteins Using Solid State NMR written by Lubica Aslimovska and published by . This book was released on 2007 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Solid State NMR Studies of Globular and Membrane Proteins written by Sorin Luca and published by . This book was released on 2003 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
