Download or read book The Pharmacology of the Mechanosensitive Channels of Escherichia Coli written by Thom Ngoc Minh Nguyen and published by . This book was released on 2007 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mechanosensitive (MS) channels are a class of ion channels which are gated by membrane stretch. The mechanosensitive channel of large conductance (MscL) of the bacterium E.coli has become a prototype MS channel for studying structure-function relationships in this class of ion channels. MscL homologues have commonly been found in Gram-negative and Gram-positive bacterial strains forming a sub-family of a larger family of MS class of ion channels encompassing prokaryotes (bacteria and archaea) as well as cell-walled eukaryotes (fungi and plants). MscL and its homologues have been found to play an important role in osmoregulation of bacterial cells. Though the MS channels of bacteria have been thoroughly studied, little is known about the pharmacology of these channels. This thesis has one general aim, that is, to identify compounds which are able to gate and/or alter the gating of the MS channels of bacteria in particular, the MscL of E. coli. Using the patch-clamp technique, potential compounds mostly identified via in-silico techniques were examined to observe the effects on MscL reconstituted in artificial lipid membranes and MscS in giant bacterial spheroplasts. The compounds were tested for the ability to spontaneously gate the MscL and MscS and/or alter the Boltzmann distribution parameters of the MscL, indicative of an effect on the gating of MscL. Compounds showing potential as MscL activators were then examined for in-vivo effects using different growth assays. The effects of parabens, gallates, eriochrome cyanine R, brilliant green, deoxycholic acid are reported. Of these compounds, parabens and eriochrome cyanine R showed the most encouraging results. Identification of MS channel gate ligands not only benefits structural studies as tetrodotoxin has for the voltage-sensitive sodium channel, these compounds could also V potentially serve as base compounds for novel antibiotics which would target the MS channels of bacteria. Since the MS channels of bacteria serve as safety valves for the bacterium, gating during exposure to a hypo-osmotic challenge such as rain to release excessive cellular turgor, a pharmacological agent that could impair the gating of the MS channels releasing essential cytoplasmic osmolytes, would cause the growth impairment or death of the bacterium. With the rise in multi-drug resistant bacteria, continual development of novel antibiotics is crucial.
Download or read book Mechanosensitive Channels in the Cytoplasmic Membrane of Escherichia Coli and Relationship to a Potassium Efflux System and to Osmotaxis written by Changhai Cui and published by . This book was released on 1996 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mechanosensitive Ion Channels and Outer Membrane Permeability of Escherichia Coli written by Matthew John Buechner and published by . This book was released on 1990 with total page 574 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mechanosensitive Channels in Escherichia Coli written by Heloisa Filus Galbiati Belmonte and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mechanosensitive Ion Channels Part A written by and published by Elsevier. This book was released on 2011-09-21 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Topics in Membranes provides a systematic, comprehensive, and rigorous approach to specific topics relevant to the study of cellular membranes. Each volume is a guest edited compendium of membrane biology. This series has been a mainstay for practicing scientists and students interested in this critical field of biology. Articles covered in the volume include The Mechanical Properties of Bilayers; Molecular Dynamic Modeling of MS Channels; Structures of the Prokaryotic Mechanosensitive; Channels MscL and MscS; 3.5 Billion Years of Mechanosensory Transduction: Structure and Function of Mechanosensitive Channels in Prokaryotes; Activation of Mechanosensitive Ion Channels by Forces Transmitted through Integrins and the Cytoskeleton; Thermodynamics of Mechanosensitivity; Flexoelectricity and Mechanotransduction; Lipid Effects on Mechanosensitive Channels; Functional Interactions of the Extracellular Matrix with Mechanosensitive Channels; MSCL: The Bacterial Mechanosensitive Channel of Large Conductance; The Bacterial Mechanosensitive Channel MscS: Emerging Principles of Gating and Modulation; Structure function relations of MscS; The MscS Cytoplasmic Domain and its Conformational Changes upon the Channel Gating; Microbial TRP Channels and Their Mechanosensitivity; MSCS-Like Proteins in Plants; Delivering Force and Amplifying Signals in Plant Mechanosensing; MS Channels in Tip Growing Systems.
Download or read book Mechanosensitive Ion Channels written by Andre Kamkin and published by Springer Science & Business Media. This book was released on 2007-10-06 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book explores the latest data dealing with mechanosensitive channels research results. It was compiled by a group of internationally recognized scientists leading in the field of mechanosensitive ion channels or mechanically gated channels and signaling cascades research. Key problems of cell mechanobiology are also discussed. As a whole, the volume dwells on the major issues of mechanical stress influencing the ion channels and intracellular signaling pathways.
Download or read book Piezo Channels written by and published by Academic Press. This book was released on 2017-07-17 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: Piezo Channels, Volume 79, the latest volume in the Current Topics in Membranes series provides the necessary membrane research to assist readers in discovering the current state of a particular field and future directions. New chapters in the updated volume include A Tour de Force: The Discovery, Properties, and Function of Piezo Channels, Piezo1 Channels in Vascular Development and the Sensing of Shear Stress, the Origin of the Force: The Force-From-Lipids Principle Applied to Piezo Channels, Genetic Diseases of PIEZO1 and PIEZO2 Dysfunction, and The Structural Basis for Sensing by the Piezo1 Protein. Users of this series will find an up-to-date presentation of the current knowledge in the field of Piezo Channels. - Written by leading experts in the field - Contains original material, both textual and illustrative, that make it a very relevant reference - Presented in a very comprehensive manner - Ideal reference for both researchers in the field and general readers who will find this book to be relevant and up-to-date
Download or read book Mechanosensitive Ion Channels Part B written by and published by Elsevier. This book was released on 2011-09-21 with total page 610 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Topics in Membranes provides a systematic, comprehensive, and rigorous approach to specific topics relevant to the study of cellular membranes. Each volume is a guest edited compendium of membrane biology. This series has been a mainstay for practicing scientists and students interested in this critical field of biology. Articles covered in the volume include ENaC Proteins in Vascular Smooth Muscle Mechanotransduction; Regulation of the Mechano-Gated K2P Channel TREK-1 by Membrane Phospholipids; MechanoTRPs and TRPA1; TRPC; The Cytoskeletal Connection to Ion Channels as a Potential Mechanosensory Mechanism. Lessons From Polycystin-2 (TRPP2); Lipid Stress at Play: Mechanosensitivity of Voltage-Gated Channels; Hair Cell Mechanotransduction: The Dynamic Interplay between Structure and Function; Pharmacology of Hair Cell MS Channels; Hair Cell Mechanotransduction; Models of Hair Cell Mechanotrasduction; Touch; Mechanosensitive Ion Channels in Dystrophic Muscle; Mechanotransduction in Endothelial Cells;MS Channels in Tumor Cell Migration; Mechanosensitive Channels in Regulating Smooth Muscle Contraction in the GI; Mechanosensitive Ion Channels in Blood-Pressure-Sensing Baroreceptor Neurons.
Download or read book Analysis of the Mechanosensitive Channels of Escherichia Coli and Their Role in Sensitivity to Parabens and Amphipaths written by Marie Viktoria Svensson and published by . This book was released on 2003 with total page 81 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Gating Mechanism of the Large Mechanosensitive Channel in Escherichia Coli and Effects of Gain of function Mutations written by and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Structural Basis for Function of the Escherichia Coli Mechanosensitive Channel of Small Conductance MscS written by Bradley Chun-Yee Akitake and published by . This book was released on 2007 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 'small' mechanosensitive channel, MscS, resides in cytoplasmic membranes of most free-living bacteria. MscS is gated directly by membrane tension and functions as an osmolyte release valve in bacterial turgor regulation. In contrast to previously studied MscL, which is a strictly prokaryotic molecule, MscS homologs are found in eukaryotes increasing the value of this channel as a general model for gating by membrane stretch.
Download or read book Thermodynamics of Membrane Receptors and Channels written by Meyer B. Jackson and published by CRC Press. This book was released on 1992-11-18 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thermodynamics of Membrane Receptors and Channels synthesizes a wealth of new information regarding the biophysics of membrane proteins. New insights provided by molecular genetics, single channel recording, and high resolution structural techniques are discussed from a conceptual perspective. Basic theoretical topics are introduced, developed, and then extensively illustrated with recent results from the literature or data from the authors' own laboratories. Theoretical and experimental information is incorporated into in-depth discussions of ion permeation mechanisms, ion channel and receptor conformational changes, aggregate activity of complexes of lipids and proteins, and how coupling is achieved between different energy modes in the many transduction systems residing in biomembranes. Thermodynamics of Membrane Receptors and Channels will be valuable both as a learning aid and a reference for biophysicists, neuroscientists, cell biologists, physiologists, and other researchers investigating any aspects of biomembranes.
Download or read book The Effect of Low Intensity Ultrasound on Mechanosensitive Channel in Escherichia Coli written by Sharon Barzilai Allon and published by . This book was released on 2011 with total page 65 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mechanical Stretch and Cytokines written by Andre Kamkin and published by Springer Science & Business Media. This book was released on 2011-09-06 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the latest findings in the field of investigation of molecular mechanisms of mechanical stretch and the role of cytokines in response of different tissues to it. On the one hand this Volume demonstrates how mechanical stretch enhances cytokines production. It describes how cytokines influence tissues and cells on a background of a mechanical stretching. It provides a description of how cells in different tissues are activated by stretch and cytokines via various signaling pathways, and how they change their gene expression. The book is a unique collection of reviews outlining current knowledge and future developments in this rapidly growing field. Knowledge of biomechanics, and mechanisms which underlie it on molecular, cellular and tissue, is necessary for understanding of the normal functioning of living organisms and allows to predict changes, which arise due to alterations of their environment.
Download or read book Organellar Ion Channels and Transporters written by Barbara E. Ehrlich and published by . This book was released on 1996 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Structural and Functional Analysis of the Escherichia Coli Small Mechanosensitive Channel MscS written by Sally Rebecca Dennison and published by . This book was released on 2004 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of Bacterial Virulence written by Michael L. Vasil and published by American Society for Microbiology Press. This book was released on 2012-12-05 with total page 1189 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive compendium of scholarly contributions relating to bacterial virulence gene regulation. • Provides insights into global control and the switch between distinct infectious states (e.g., acute vs. chronic). • Considers key issues about the mechanisms of gene regulation relating to: surface factors, exported toxins and export mechanisms. • Reflects on how the regulation of intracellular lifestyles and the response to stress can ultimately have an impact on the outcome of an infection. • Highlights and examines some emerging regulatory mechanisms of special significance. • Serves as an ideal compendium of valuable topics for students, researchers and faculty with interests in how the mechanisms of gene regulation ultimately affect the outcome of an array of bacterial infectious diseases.
