Download or read book Mechanistic Studies of Thermobifida Fusca Exocellulase Cel6B written by Thu Van Vuong and published by . This book was released on 2010 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding the mechanism by which cellulases catalyze cellulose hydrolysis can greatly contribute to the development of biofuels. The thermophilic bacterium Thermobifida fusca, a major degrader of plant cell walls in certain environments, secretes seven different cellulases including exocellulase Cel6B. This cellulase acts by an inverting mechanism; however, its catalytic acid and base residues had not been identified. Biochemical approaches confirmed D274 to be the catalytic acid residue. A single catalytic base residue could not be determined, as sodium azide assays showed no activity rescue for any single mutations of candidate residues. However, a double mutation of D226A and S232A knocked out enzymatic activity and its activity was partially rescued by sodium azide. We therefore propose a novel hydrolysis mechanism for T. fusca Cel6B involving a proton-transferring network to carry out the catalytic base function. T. fusca exocellulase Cel6B was also engineered to gain knowledge on the relationship between processivity and synergism as these properties are important for hydrolyzing crystalline cellulose. Mutations of several residues in the active site tunnel of Cel6B gave higher processivity. This improvement was confirmed by two assays: the ratio of soluble/insoluble reducing sugars as well as the ratio of oligosaccharide products. Surprisingly, the mutant enzyme, which has the highest processivity, showed the least synergism in mixtures with endocellulases, suggesting that improving exocellulase processivity might not always be an effective strategy for producing improved cellulase mixtures for biomass conversion. The highly processive Cel6B mutant enzymes were successfully fluorescently labeled, so these species can be used to visualize binding and track their movement on cellulose. The catalytic domains of Cel6B was found to bind non-productively to other polysaccharides; therefore, the balance between specific binding and non-specific adsorption should be always considered when engineering cellulases for hydrolyzing complex substrates. Using immuno-precipitation, Cel6B was demonstrated to contribute greatly to the hydrolysis of crystalline cellulose by T. fusca.
Download or read book Direct Microbial Conversion of Biomass to Advanced Biofuels written by Michael E Himmel and published by Elsevier. This book was released on 2015-05-19 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt: 'Direct Microbial Conversion of Biomass to Advanced Biofuels' is a stylized text that is rich in both the basic and applied sciences. It provides a higher level summary of the most important aspects of the topic, addressing critical problems solved by deep science. Expert users will find new, critical methods that can be applied to their work, detailed experimental plans, important outcomes given for illustrative problems, and conclusions drawn for specific studies that address broad based issues. A broad range of readers will find this to be a comprehensive, informational text on the subject matter, including experimentalists and even CEOs deciding on new business directions. - Describes an important new field in biotechnology, the consolidated conversion of lignocellulosic feedstocks to advanced fuels - Up-to-date views of promising technologies used in the production of advanced biofuels - Presents the newest ideas, well-designed experiments, and outcomes - Provides outstanding illustrations from NREL and contributing researchers - Contains contributions from leaders in the field that provide numerous examples and insights into the most important aspects of the topic
Download or read book Biomass Recalcitrance written by Michael Himmel and published by Wiley-Blackwell. This book was released on 2008-06-23 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines the connection between biomass structure, ultrastructure, and composition, to resistance to enzymatic deconstruction, with the aim of discovering new cost-effective technologies for biorefineries. It contains chapters on topics extending from the highest levels of biorefinery design and biomass life-cycle analysis, to detailed aspects of plant cell wall structure, chemical treatments, enzymatic hydrolysis, and product fermentation options."--Pub. desc.
Download or read book Modeling and Analysis of Cellulose Hydrolysis by Thermobifida Fusca CEL5A CEL6B and CEL9A and Binary Mixtures of These Cellulases written by Tina Jeoh and published by . This book was released on 2004 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bioethanol and Natural Resources written by Ruben Michael Ceballos and published by CRC Press. This book was released on 2017-11-22 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioethanol and Natural Resources: Substrates, Chemistry and Engineered Systems provides a comprehensive review of feedstocks, physiochemical and biological pretreatments, molecular substrates, cellulolytic and ligninolytic enzymes, and advanced technologies for producing bioethanol. Although this book provides a review of first-generation bioethanol feedstocks, chemistry, and processes, there is an emphasis on second-generation "cellulosic" ethanol production. With rapid advances in biofuels technologies and the continued global dependency on unsustainable extraction of fossil fuels, this text is timely. Although it is intended to be used as a supplemental text for advanced undergraduate or graduate level courses, the book is accessible to a non-academic audience. This book provides a unique opportunity to understand bioethanol production from the basic concepts and processes to the most cutting-edge technologies under development.
Download or read book Directory of Graduate Research written by and published by . This book was released on 2001 with total page 1846 pages. Available in PDF, EPUB and Kindle. Book excerpt: Faculties, publications and doctoral theses in departments or divisions of chemistry, chemical engineering, biochemistry and pharmaceutical and/or medicinal chemistry at universities in the United States and Canada.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 2000 with total page 1800 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Class 3 2 Hydrolases VIII written by Dietmar Schomburg and published by Springer Science & Business Media. This book was released on 2003-06-18 with total page 652 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Springer Handbook of Enzymes provides concise data on some 5,000 enzymes sufficiently well characterized – and here is the second, updated edition. Their application in analytical, synthetic and biotechnology processes as well as in food industry, and for medicinal treatments is added. Data sheets are arranged in their EC-Number sequence. The new edition reflects considerable progress in enzymology: the total material has more than doubled, and the complete 2nd edition consists of 39 volumes plus Synonym Index. Starting in 2009, all newly classified enzymes are treated in Supplement Volumes.
Download or read book Aqueous Pretreatment of Plant Biomass for Biological and Chemical Conversion to Fuels and Chemicals written by Charles E. Wyman and published by John Wiley & Sons. This book was released on 2013-03-27 with total page 597 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant biomass is attracting increasing attention as a sustainable resource for large-scale production of renewable fuels and chemicals. However, in order to successfully compete with petroleum, it is vital that biomass conversion processes are designed to minimize costs and maximize yields. Advances in pretreatment technology are critical in order to develop high-yielding, cost-competitive routes to renewable fuels and chemicals. Aqueous Pretreatment of Plant Biomass for Biological and Chemical Conversion to Fuels and Chemicals presents a comprehensive overview of the currently available aqueous pretreatment technologies for cellulosic biomass, highlighting the fundamental chemistry and biology of each method, key attributes and limitations, and opportunities for future advances. Topics covered include: • The importance of biomass conversion to fuels • The role of pretreatment in biological and chemical conversion of biomass • Composition and structure of biomass, and recalcitrance to conversion • Fundamentals of biomass pretreatment at low, neutral and high pH • Ionic liquid and organosolv pretreatments to fractionate biomass • Comparative data for application of leading pretreatments and effect of enzyme formulations • Physical and chemical features of pretreated biomass • Economics of pretreatment for biological processing • Methods of analysis and enzymatic conversion of biomass streams • Experimental pretreatment systems from multiwell plates to pilot plant operations This comprehensive reference book provides an authoritative source of information on the pretreatment of cellulosic biomass to aid those experienced in the field to access the most current information on the topic. It will also be invaluable to those entering the growing field of biomass conversion.
Download or read book Biofuels written by Jonathan R. Mielenz and published by Humana Press. This book was released on 2012-03-07 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: This editor believes the public finally understands that bioenergy should be a critical new 39 component of our world’s future energy supply. While this new enlightenment might have 40 come from bioenergy’s environmentally friendly attributes and its renewable and sustain- 41 able nature, the reality is that the primary driver has been both the high cost of energy, 42 led recently by petroleum products, and the unique climatic changes such as local weather 43 events and reports of melting of ancient glaciers. I believe that much of the public under- 44 stands the primary cause of these changes is the growing demand for fossil fuels for heat- 45 ing and transportation needs driven by both population increases and dramatic economic 46 development around the globe. This increased demand, as well as global politics, has 47 impacted the costs for these energy products. At the same time, increased use of fossil fuels 48 has been liberating unprecedented levels of CO and other greenhouse gasses (GHGs) 49 2 that must be impacting the global climate. As a result, the world is looking for alternative 50 solutions. Renewable energy is one of the leading options as long as it is pursued in a sus- 51 tainable fashion. Of course, renewable energy has numerous sources, including wind, solar, 52 geothermal, ocean currents/tides, and bio-based energy. The latter category of renewable 53 energy is centered primarily on use of green plant matter, i. e.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2008 with total page 906 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Secretions and Exudates in Biological Systems written by Jorge M. Vivanco and published by Springer Science & Business Media. This book was released on 2012-01-21 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Secretions and emissions in biological systems play important signaling roles within the organism but also in its communications with the surrounding environment. This volume brings together state-of-the-art information on the role of secretions and emissions in different organs and organisms ranging from flowers and roots of plants to nematodes and human organs. The plant chapters relate information regarding the biochemistry of flower volatiles and root exudates, and their role in attracting pollinators and soil microbial communities respectively. Microbial chapters explain the biochemistry and ecology of quorum sensing and how microbial communities highly co-adapted to plants can aid in bio-energy applications by degrading ligno-cellulosic materials. Other chapters explain the biology of secretions by nematodes, algae and humans, among other organisms. This volume will be a welcome addition to the literature, as no other book covers aspects related to biological secretion in such a holistic and integrative manner.
Download or read book Sustainable Degradation of Lignocellulosic Biomass written by Anuj Chandel and published by BoD – Books on Demand. This book was released on 2013-05-15 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides important aspects of sustainable degradation of lignocellulosic biomass which has a pivotal role for the economic production of several value-added products and biofuels with safe environment. Different pretreatment techniques and enzymatic hydrolysis process along with the characterization of cell wall components have been discussed broadly. The following features of this book attribute its distinctiveness: This book comprehensively covers the improvement in methodologies for the biomass pretreatment, hemicellulose and cellulose breakdown into fermentable sugars, the analytical methods for biomass characterization, and bioconversion of cellulosics into biofuels. In addition, mechanistic analysis of biomass pretreatment and enzymatic hydrolysis have been discussed in details, highlighting key factors influencing these processes at industrial scale.
Download or read book Cellulose written by Theo G.M. Van De Ven and published by BoD – Books on Demand. This book was released on 2013-08-28 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cellulose is destined to play a major role in the emerging bioeconomy. Awareness of the environment and a depletion of fossil fuels are some of the driving forces for looking at forest biomaterials for an alternative source of energy, chemicals and materials. The importance of cellulose is widely recognized world-wide and as such the field of cellulose science is expanding exponentially. Cellulose, the most abundant biopolymer on earth, has unique properties which makes it an ideal starting point for transforming it into useful materials. To achieve this, a solid knowledge of cellulose is essential. As such this book on cellulose, the first in a series of three, is very timely. It deals with fundamental aspect of cellulose, giving the reader a good appreciation of the richness of cellulose properties. Book Cellulose - Fundamental Aspects is a good introduction to books Cellulose - Medical, Pharmaceutical and Electronic Applications and Cellulose - Biomass Conversion , in which applications of cellulose and its conversion to other materials are treated.
Download or read book Biomass Conversion written by Michael E. Himmel and published by Humana Press. This book was released on 2012-07-28 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomass conversion research is a combination of basic science, applied science, and engineering testing and analysis. Conversion science includes the initial treatment (called pre-treatment) of the feedstock to render it more amenable to enzyme action, enzymatic saccharification, and finally product formation by microbiological or chemical processes. In Biomass Conversion: Methods and Protocols, expert researchers in the field detail methods which are now commonly used to study biomass conversion. These methods include Biomass Feedstocks and Cellulose, Plant Cell Wall Degrading Enzymes and Microorganisms, and Lignins and Hemicelluloses. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting informed, reproducible results in the laboratory.
Download or read book Enzyme Biocatalysis written by Andrés Illanes and published by Springer Science & Business Media. This book was released on 2008-06-19 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book was written with the purpose of providing a sound basis for the design of enzymatic reactions based on kinetic principles, but also to give an updated vision of the potentials and limitations of biocatalysis, especially with respect to recent app- cations in processes of organic synthesis. The ?rst ?ve chapters are structured in the form of a textbook, going from the basic principles of enzyme structure and fu- tion to reactor design for homogeneous systems with soluble enzymes and hete- geneous systems with immobilized enzymes. The last chapter of the book is divided into six sections that represent illustrative case studies of biocatalytic processes of industrial relevance or potential, written by experts in the respective ?elds. We sincerely hope that this book will represent an element in the toolbox of gr- uate students in applied biology and chemical and biochemical engineering and also of undergraduate students with formal training in organic chemistry, biochemistry, thermodynamics and chemical reaction kinetics. Beyond that, the book pretends also to illustrate the potential of biocatalytic processes with case studies in the ?eld of organic synthesis, which we hope will be of interest for the academia and prof- sionals involved in R&D&I. If some of our young readers are encouraged to engage or persevere in their work in biocatalysis this will certainly be our more precious reward.
Download or read book Marine Enzymes Biotechnology Production and Industrial Applications Part II Marine Organisms Producing Enzymes written by and published by Academic Press. This book was released on 2016-12-01 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: Marine Enzymes Biotechnology: Production and Industrial Applications, Part II - Marine Organisms Producting Enzymes
