Download or read book Mechanisms of Zebrafish Development and Disease written by Michael J. Jurynec and published by . This book was released on 2006 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Zebrafish at the Interface of Development and Disease Research written by and published by Academic Press. This book was released on 2017-03-21 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zebrafish at the Interface of Development and Disease Research presents the latest on the shared pathways that govern development and contribute to disease. Zebrafish have traditionally been used to study vertebrate development, providing interesting data on the developmental processes and genes that are implicated in disease. This new release in the series contains informative discussions on congenital heart defects in zebrafish, the use of zebrafish in studying kidney development and disease, and muscle development, homeostasis and disease in zebrafish. Each chapter interweaves the study of zebrafish development and its application to the immune system, the kidney, liver, heart and others. The subject matter is unique - no single volume has pulled together the theme of development and disease with zebrafish as a centerpiece Leading experts in the field - all of them running zebrafish focused labs for each chapter

Download or read book Zebrafish in Development and Disease written by Gokhan Dalgin and published by Frontiers Media SA. This book was released on 2019-12-06 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt: There are only a few vertebrate systems that can be used to model human diseases for biomedical discovery. The zebrafish model provides key advantages over existing models. Their externally developing embryos provide high-throughput non-invasive imaging, chemical screening, forward and reverse genetics, and their regeneration capacity make zebrafish a valuable system for novel discovery. Developmental studies using zebrafish has influenced discoveries in many human health-related conditions. This Research Topic covers all aspects of zebrafish studies, providing developmental mechanisms to human health conditions. The aim of the Research Topic was to foster a platform to bring all levels of zebrafish research including but not limited to development, disease, regeneration, drug screening, bioinformatics and Omics studies.

Download or read book Investigating mechanisms of angiogenesis in health and disease using zebrafish models written by Zaheer Ali and published by Linköping University Electronic Press. This book was released on 2018-12-07 with total page 49 pages. Available in PDF, EPUB and Kindle. Book excerpt: Angiogenesis, the growth of blood vessels from an existing vasculature, can occur by sprouting from preexisting vessels or by vessel splitting (intussusception). Pathological angiogenesis drives choroidal neovascularization (CNV) in age related macular degeneration (AMD) which is commonly restricted under the retinal pigment epithelium (RPE), called occult CNV, but may also involve vessels penetrating through the RPE into the sub-retinal space. Pathological vessels are poorly developed, insufficiently perfused and highly leaky, phenotypes that are considered to drive disease progression and lead to poor prognosis. Currently, a number of anti-angiogenic drugs exists, the majority of which target vascular endothelial factor (VEGF), but although they often are highly beneficial for treating eye diseases in the short-term, they are generally of limited efficacy in other diseases such as cancer, and also have poorer efficacy when used for treatment of eye diseases in the long-term. A better understanding of the mechanisms underlying pathological angiogenesis can generate new targets for treatment leading to development of better drugs for cancer and retinopathies, but perhaps also other angiogenesis-dependent diseases, in the future. In this thesis mechanisms involved in developmental angiogenesis or pathological angiogenesis in the choroid, cornea or melanoma was identified. These findings highlight the need to further elaborate our knowledge related to angiogenesis in different tissues/conditions for a more targeted, and potentially effective treatment of diseases in the future. In paper I, we for the first time identified the choriocapillaries (CCs) in adult zebrafish and found that occult CNV could be induced by exposing the fish to severe hypoxia. Interestingly, we found that occult CNV relied on intussusception, involving not only de novo generation of intussusceptive pillars but also a previously poorly understood mechanism called pillar splitting. This involved HIF-VEGF-VEGFR2 signaling and evidence that this also occurred in both rats and humans suffering from AMD suggested that the mechanism was conserved and clinically relevant. In contrast, we found in paper II that the development of CCs in the zebrafish relies on sprouting angiogenesis, involve continuous remodeling, and delayed maturation of the vasculature in 2D. The initial development was found to occur by a unique process of tissuewide synchronized vasculogenesis. As expected, VEGFA via VEGFR2 was also critical for the development of these vessels in the zebrafish embryo, but surprisingly this was independent on hypoxia-inducible factor (HIF)-1. Inflammatory nuclear factor-kB (NF-kB) signaling is involved in the progression of angiogenesis, but this signaling pathway has mainly been studied in the inflammatory cells and the role of NF-kB in the endothelial cells during angiogenesis is poorly understood. In paper III, we found that blocking NF-kB signaling using a specific IKK2 blocker IMD0354, specifically blocks pathological as well as developmental angiogenesis by targeting endothelial cell NF-kB signaling in the endothelial cells. Using a rat model for suture-induced corneal neovascularization, IMD0354 treatment lead to reduced production of inflammatory C-C motif chemokine ligand 2 (CCL2), C-X-C motif chemokine ligand 5 (CXCL5) and VEGF, and thereby reduced pathological corneal angiogenesis in this model. Using the zebrafish tumor xenograft model in paper IV, we found an association between Microphthalmia associated transcription factor (MITF) and pigment epithelium derived factor (PEDF), which was involved in pathological tumor angiogenesis and metastasis. Similarly, in paper V we used zebrafish transplantation models to study and investigate the use of biocompatible polymers for the delivery of pro-angiogenic FGF-2 as a potential treatment strategy for ischemic diseases such as myocardial infarction (MI). Conclusively, this thesis provides new insights into diverse fields of angiogenic assays using zebrafish, and reveals new mechanisms of angiogenesis in health and disease. This work will hopefully provide a foundation for further studies into occult CNV related to AMD, a process that has not been possible to study previously in pre-clinical models. In addition, zebrafish xenograft or other transplantation models used in this work will likely be important to study cancer biology and to develop more attractive pharmaceutical preparations based on biocompatible hydrogels formulated as microspheres in the future.

Download or read book Live Imaging In Zebrafish Insights Into Development And Disease written by Karuna Sampath and published by World Scientific. This book was released on 2010-08-30 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: The zebrafish embryo has now been well established as an organism of choice for the genetic and cell biological basis of vertebrate development. Large-scale forward genetic screens together with reverse genetic tools have resulted in a flood of new information on the regulation of vertebrate development. Furthermore, the unique transparency of the zebrafish embryo allows unprecedented access to cells and tissues and many important discoveries on gene function have already been made using live imaging techniques in this organism. This book successfully brings together state-of-the-art methodologies of live imaging, such as the use of fluorescence correlation spectroscopy (FCS), that will be useful for investigators in the field of developmental biology to address their favorite problem using cell biological approaches in the zebrafishOne of the key reasons that has made the zebrafish such a popular vertebrate model is its transparency and its potential for high-resolution cell biology and imaging. However, there is no comprehensive and up-to-date guide to the new “live imaging” tools that can now be used in the zebrafish. Furthermore, there are many new microscopy methods that have been adapted for use in zebrafish, and this book can be a good guide even for scientists already familiar with the imaging advantages of this model.

Download or read book The Zebrafish in Biomedical Research written by Samuel Cartner and published by Academic Press. This book was released on 2019-11-22 with total page 720 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Zebrafish in Biomedical Research: Biology, Husbandry, Diseases, and Research Applications is a comprehensive work that fulfills a critical need for a thorough compilation of information on this species. The text provides significant updates for working vivarium professionals maintaining zebrafish colonies, veterinarians responsible for their care and well-being, zoologists and ethologists studying the species, and investigators using the species to gain critical insights into human physiology and disease. As the zebrafish has become an important model organism for the study of vertebrate development and disease, organ function, behavior, toxicology, cancer, and drug discovery, this book presents an important resource for future research. Presents a complete view of the zebrafish, covering their biology, husbandry, diseases and research applications Includes the work of world-renowned authors Provides the first authoritative and comprehensive treatment of zebrafish in biomedical research as part of the ACLAM series

Download or read book Cancer and Zebrafish written by David M. Langenau and published by Springer. This book was released on 2016-05-10 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on defining the unique attributes of using the zebrafish cancer model for discovering important pathways and potential drug targets for the treatment of human cancers. Using the zebrafish model, the volume explores oncogene and tumor suppressor discovery, chemical genetic approaches, genomics, epigenetics, cancer imaging, and cell transplantation. Contributed chapters come from the most prominent laboratories working in this field, which provides a unique perspective on zebrafish models from a wide spectrum of the research community. In addition, the book offers a detailed analysis of the most current research in the area for specific zebrafish cancer models, including T cell leukemia, rhabdomyosarcoma, liver and pancreatic cancer, melanoma, neuroblastoma, germ cell tumors, and malignant peripheral sheath tumors. A chapter is also dedicated to the development and utilization of other piscine models of cancer. The compilation of chapters in the volume culminates into a comprehensive and definitive text on zebrafish and cancer, providing a much needed resource on the powerful attributes of the zebrafish model system.

Download or read book Molecular Mechanisms of Glia in Development and Disease written by Ryan B. MacDonald and published by Frontiers Media SA. This book was released on 2023-04-11 with total page 533 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Intrinsic and Extrinsic Mechanisms of Nodal Signaling Regulation During Zebrafish Development written by Christopher Everett Slagle and published by . This book was released on 2011 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Heart Development and Disease written by Benoit G. Bruneau and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "Development of the heart is a complex process and can lead to serious congenital disease if the process goes awry. This book provides a detailed description of the cell lineages involved in heart development and how their migration and morphogenesis are controlled. It also examines the genetic and environmental bases for congenital heart disease and how model systems are revealing more about the processes involved"--

Download or read book Molecular mechanisms in ocular development and disease written by Rajalekshmy Shyam and published by Frontiers Media SA. This book was released on 2023-07-04 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Recent Advances in Zebrafish Researches written by Yusuf Bozkurt and published by BoD – Books on Demand. This book was released on 2018-05-30 with total page 219 pages. Available in PDF, EPUB and Kindle. Book excerpt: Model organisms have been used in various disciplines in order to understand different mechanisms underlying the problems. From this point of view, the zebrafish has become a favorite model organism in different scientific research fields in recent years because of its rapid embryonic development, transparency of its embryos, and its large number of offspring along with several other advantages. Recent Advances in Zebrafish Researches demonstrates the role and the function of zebrafish in different research fields and totally includes 11 chapters, which have been written by the expert researches in their fields. With this book, every researcher will better understand different mechanisms underlying the problems at different disciplines using zebrafish as model organism.

Download or read book The Zebrafish Disease Models and Chemical Screens written by and published by Academic Press. This book was released on 2011-09-09 with total page 591 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Cell Biology is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish. Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls This volume concentrates on Disease Models and Chemical Screens

Download or read book Atlas of Zebrafish Development written by Robert Bryson-Richardson and published by Academic Press. This book was released on 2012 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: Zebrafish are widely considered an excellent model system for vertebrate development. The embryo is transparent, thereby enabling visualization and use of labelling and transgenic approaches. Moreover, because of the ease of inducing new mutations in zebrafish and similarity with the human genome, this organism may be used effectively for disease studies. For example, mutant zebrafish are being utilized for testing drugs that will combat a range of human diseases, from Alzheimer's and cancer to kidney failure and congenital heart disease. For the first time, this atlas provides the research community with a complete reference for zebrafish anatomy spanning the early embryo all the way to adulthood. The authors employ the technique of optical projection tomography (OPT), and offer a series of sections in multiple planes from each sample. The contents are organized by developmental stages, with over 200 images that contain annotations describing anatomical structures relevant to development. In addition, chapters feature explanatory text that highlights major developments in the zebrafish during each stage. Provides the first comprehensive anatomical resource that covers all regions of zebrafish anatomy from the larval period to adulthood The over 200 images include explanatory notes Each chapter contains a concise description of key anatomical features that factor in zebrafish development Despite many years of use as a model system, until now there has never been a guide to zebrafish at the larval stage The book's website contains a database of over 10k sections from different regions as well as 3D images that are interactive

Download or read book Mechanisms of Copper Deficiency in the Zebrafish Embryo written by Erik Christian Madsen and published by . This book was released on 2010 with total page 129 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proper maternal nutrition is critical for early embryonic development. Despite overwhelming epidemiologic data indicating the benefits nutrient supplementation for the developing organism we do not fully understand the genetics of predisposition to abnormal developmental phenotypes when faced with suboptimal nutrient levels. Copper is an essential nutrient required for critical biochemical processes. Severe defects in copper homeostasis lead to significant disease typified by the X-linked recessive disorder Menkes disease. Patients with Menkes disease have cutis laxa, bone deformities, hypopigmentation, arterial malformation, and neurodegeneration due to copper deficiency caused by loss-of-function mutations in ATP7A, a copper transport protein. Despite the critical requirement for adequate copper nutrition and the characterization of key copper transport proteins there remain significant gaps in our understanding of copper metabolism. In order to better understand both the cell metabolic and developmental requirements for copper our laboratory has defined a zebrafish model of severe copper deficiency. The copper chelator neocuproine causes a Menkes-like phenotype in wild-type zebrafish embryos and the mutant calamity which phenocopies neocuproine treated embryos contains a null allele of atp7a. In this thesis we build upon this previous model in two ways in order to address the problem of treatment of Menkes disease and to define novel pathways important for copper metabolism. The first body of work uses small-molecule modifiers of mRNA processing (morpholinos) to correct splicing defects which cause Menkes disease phenotypes in zebrafish embryos. Since about 20% of human disease causing mutations are due to splicing defects this approach may be directly applicable to a wide array of human diseases. The second body of work uses a forward chemical-genetic screen for zebrafish mutants sensitive to mild copper deficiency. Screening mutagenized embryos exposed to subthreshold doses of the chelator neocuproine for copper deficiency phenotypes resulted in two mutants. The first, containing a hypomorphic allele of atp7a demonstrates the effect of maternal nutrition and genetics on embryonic development. The second contains a loss-of-function mutation in the vacuolar proton pump leading to a severe embyronic lethal phenotype which is sensitive to copper chelation. This implicates pH gradients in the metabolism of copper in zebrafish.

Download or read book The Zebrafish Cellular and Developmental Biology Part B written by and published by Academic Press. This book was released on 2011-04-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Cell Biology, the second of two parts on the subject of zebrafish, provides a comprehensive compendium of laboratory protocols and reviews covering all the new methods developed since 1999.

Download or read book Examining the Mechanisms and Roles of Biologically Generated Forces in Coordinating Zebrafish Development written by Jack Chai and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue function relies on proper specification of both cell type and cellular organization. For example, the heart requires properly differentiated myocytes to contract and pump blood. Additionally, the heart also requires properly constructed chambers and valves to maintain blood flow and oxygenate entire animals. Until recently, the study of biology has mainly focused on generating the correct cell type by exposing cells to specific biochemical stimuli. However, recently, studies have shown that physical forces are also central to cellular decisions and also influence collective cell migration. While some progress has been made in understanding how physical forces influence cell signaling, very little is known about how physical forces may help shape structure formation in multicellular development. In the work presented below, we demonstrate that a simple line tension provided by a contractile ring of actin and myosin helps reorient the blastoderm in the zebrafish embryo to the nearest long axis thus positioning the head-tail axis along the nearest axis of symmetry. We further provide analytical models and simulations, based on a simple force balance, that quantitatively account for the effect of this line tension during blastoderm migration. From these analyses, we present a dimensionless quantity that can be used to determine whether a biological system has generated sufficient coordinating force to influence its structure during development. We provide further evidence showing that external mechanical forces can profoundly affect biochemical signaling processes that control the location and orientation of the dorsoventral axis. In particular, we describe findings indicating that [beta]-catenin activation, a signaling event crucial to many biological processes, is sensitive to mechanical cues as well as extracellular Ca2+. In the last section, we provide an analytical framework that describes how mechanical forces may be transduced over long length and time scales. From these experiments and analyses, we observe that mechanical force plays an integral role in coordinating biological processes in development. We also observe that the organisms and cells may employ simple mechanisms to achieve mechanotransduction over large time and length scales. Finally, we demonstrate that mechanical perturbation can be a useful tool for probing biochemical pathways that can be difficult to achieve via chemical signals.