Download or read book Matrix Metalloproteinase Protocols written by Ian M. Clark and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 547 pages. Available in PDF, EPUB and Kindle. Book excerpt: Research in the matrix metalloproteinase field began with the demonstration by Gross and Lapière, in 1962, that resorbing tadpole tail expressed an enzyme that could degrade collagen gels. These humble beginnings have led us to the elucidation of around twenty distinct vertebrate MMPs, along with a variety of homologs from such diverse organisms as sea urchin, plants, nematode worm, and bacteria. This, coupled with four known specific inhibitors of MMPs, the TIMPs, gives a complex picture. Part I of Matrix Metalloproteinase Protocols provides the reader with a selective overview of the MMP arena, and a chance to come to grips with where the field has been, where it is, and where it is going. I hope that this complements all of the methodology that comes later. Part II presents the reader with a diverse set of methods for the expression and purification of MMPs and TIMPs, bringing together the long and often hard-earned experience of a number of researchers. Part III allows the reader to detect MMPs and TIMPs at both the protein and mRNA level, whereas Part IV gives the ability to assay MMP and TIMP activities in a wide variety of circumstances.
Download or read book Matrix Metalloproteinase Protocols written by Ian M. Clark and published by Humana Press. This book was released on 2010-03-04 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms. Fully updating and adding to the popular first edition, Matrix Metalloproteinase Protocols, Second Edition includes a series of state-of-the-art techniques provided by eminent experts in the field. Beginning with a brief overview of the MMP arena, from how these enzymes fit into the larger degradome to what occurs when their expression and function in the mouse is modulated, the volume continues with sections on the expression and purification of MMPs and TIMPs, the detection of MMPs and TIMPs at both the protein and mRNA level, and our ability to assay MMP and TIMP activities in a wide variety of circumstances. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Matrix Metalloproteinase Protocols, Second Edition is an ideal source for many of the essential laboratory techniques for both novice and seasoned researchers alike collected in one convenient volume.
Download or read book Matrix Metalloproteases written by Charles A. Galea and published by Humana. This book was released on 2018-08-07 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the identification of the first matrix metalloprotease (insterstitial collagenase or MMP-1) more than 20 closely related and evolutionarily conserved vertebrate MMPs have been discovered. Chapters in this volume detail methods and protocols on recent advances in the expression and purification of MMPs, biophysical methods such as X-ray crystallography, NMR spectroscopy and small angle X-ray scattering, computational and experimental methods for determining MMP specificity, methods for detecting MMP activity in vitro and in vivo, and methods for the development and characterization of MMP-based inhibitors as potential therapeutics for the treatment of various diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Matrix Metalloproteases: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Download or read book Matrix Metalloproteinases written by and published by Elsevier. This book was released on 1998-03-27 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: The chapters in this book thoroughly cover the structure, regulation, and function of matrix metalloproteinases, and provide information on the latest strategies to inhibit enzyme activity. This work will be an indispensable reference tool for investigators with an interest in extracellular matrix biology, matrix turnover, enzymology and biochemistry of proteinases, developmental biology, pathology, and therapeutic interventions. Provides state-of-the-art information on a field with broad implications to many areas of biology Includes detailed coverage of the structure and regulation of all major matrix metalloproteinases Chapters focus on a timely and expanding field Topics have direct relevance to understanding human disease pathology of cancer, arthritis, and vascular disease Discusses latest strategies used in the development of new therapeutics to inhibit metalloproteinase activity
Download or read book Wound Healing written by Vlad Alexandrescu and published by BoD – Books on Demand. This book was released on 2016-10-12 with total page 546 pages. Available in PDF, EPUB and Kindle. Book excerpt: Outstanding scientific advances over the last decades unceasingly reveal real complexity of wound-healing process, astonishing in its staged progression, as life is unfolding itself. This natural course of tissue repair seems to bear thousands of overlapping molecular and macroscopic processes that nowadays only start to unfold to our knowledge. The present volume collecting recent scientific references proposes to readers a two-folded audacious goal. First, an updated design of intimate cellular mechanisms is entailed in tissue regeneration that emanates from the first section of the book. Next, a multidisciplinary therapeutic perspective that focuses on macroscopic healing throughout the second part of this work adds clinically integrated observation. Practical diagnostic and treatment information is appended in each chapter that may equally help experienced clinicians or dedicated students and researchers in broadening essential breaking points of their work. It is the wish of all multidisciplinary experts who gather prominent author's panel of this volume to incorporate latest medical reports and compel limits of current understanding for better tissue regeneration, limb salvage, and improved quality of life of our patients.
Download or read book Inflammation Protocols written by Paul G. Winyard and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 373 pages. Available in PDF, EPUB and Kindle. Book excerpt: Inflammation has been described as the basis of many pathologies of human disease. When one considers the updated signs of inflammation, they would be vasodilation, cell migration, and, in the case of chronic inflam- tion, cell proliferation, often with an underlying autoimmune basis. Gen- ally, inflammation may be divided into acute, chronic, and autoimmune, - though the editors believe that most, if not all, chronic states are often the result of an autoimmune response to an endogenous antigen. Thus, a proper understanding of the inflammatory basis may provide clues to new therap- tic targets not only in classical inflammatory diseases, but atherosclerosis, cancer, and ischemic heart disease as well. The lack of advances in classical inflammatory diseases, such as rh- matoid arthritis, may in part arise from a failure to classify the disease into different forms. That different forms exist is exemplified in patients with d- fering responses to existing antiinflammatory drugs, ranging from nonresponders to very positive responders for a particular nonsteroidal an- inflammatory drug (NSAID). Though researchers have progressively unr- eled the mechanisms, the story is far from complete. It should also be noted that the inflammatory response is part of the innate immune response, or to use John Hunter’s words in 1795, “inflammation is a salutary response.” That may be applied in particular to the defensive response to invading micro- ganisms.
Download or read book Zymography written by Jeff Wilkesman and published by Humana. This book was released on 2018-06-12 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels. Sections cover subjects such as endopeptidase zymography, reverse zymography & in situ zymography, 2D zymography, as well as a variety of special cases. Written in the highly respected Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Zymography: Methods and Protocols serves as an ideal aid for a broad array of researchers from the fields of biological and medicinal science, as well as scientists working in clinical and diagnostic medicine, medical genetics, agricultural entomology, genetic monitoring of environmental pollution, and forensic science.
Download or read book Matrix Metalloproteinase Biology written by Irit Sagi and published by John Wiley & Sons. This book was released on 2015-05-18 with total page 253 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discussing recent advances in the field of matrix metalloproteinase (MMP) research from a multidisciplinary perspective, Matrix Metalloproteinase Biologyis a collection of chapters written by leaders in the field of MMPs. The book focuses on the challenges of understanding the mechanisms substrate degradation by MMPs, as well as how these enzymes are able to degrade large, highly ordered substrates such as collagen. All topics addressed are considered in relation to disease progression including roles in cancer metastasis, rheumatoid arthritis and other inflammatory diseases. The text first provides an overview of MMPs, focusing on the history, the development and failures of small molecule inhibitors in clinical trials, and work with TIMPS, the endogenous inhibitors of MMPs. These introductory chapters establish the foundation for later discussion of the recent progress on the design of different types of inhibitors, including novel antibody based therapeutics. The following section emphasizes research using novel methods to further the study of the MMPs. The third and final section focuses on in vivo research, particularly with respect to cancer models, degradation of the extracellular matrix, and MMP involvement in other disease states. Written and edited by leaders in the field, Matrix Metalloproteinase Biology addresses the rapidly growth in MMP research, and will be an invaluable resource to advanced students and researchers studying cell and molecular biology.
Download or read book The Role of Matrix Metalloproteinase in Human Body Pathologies written by Francesco Travascio and published by BoD – Books on Demand. This book was released on 2017-12-20 with total page 202 pages. Available in PDF, EPUB and Kindle. Book excerpt: Matrix metalloproteinases (MMPs) are a family of proteolytic zinc-containing enzymes involved in physiological as well as in pathological processes in the human organism. MMPs play a key role in the remodeling of the extracellular matrix. Such a process may occur because of tissue homeostasis, morphogenesis, and tissue repair. However, remodeling could also be a part of many pathological states such as arthritis, cardiovascular diseases, neurodegenerative diseases, or impaired development in congenital anomalies. This book overviews the role of MMPs in different pathologies affecting the human body.
Download or read book Proteases and Cancer written by Santiago Cal and published by Humana Press. This book was released on 2018-01-10 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the role of proteases in various catalytic classes and cellular processes associated with cancer. The chapters in this book cover topics such as encoding proteolytic enzymes, cleavage sites of proteases, functional catalytic domains of MMPs, zymography, angiogenesis and lymphangiogenesis, genetic and epigenetic alterations of metallopeptidases, and methods to target metalloproteinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting edge, Proteases and Cancer: Methods and Protocols is a valuable resource for clinical researchers across numerous fields such as molecular and cellular biology, oncology, biochemistry, and chemistry. This book is also useful for PhD students in the various disciples of health sciences.
Download or read book Matrix Metalloproteinase Biology written by Irit Sagi and published by John Wiley & Sons. This book was released on 2015-08-03 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discussing recent advances in the field of matrix metalloproteinase (MMP) research from a multidisciplinary perspective, Matrix Metalloproteinase Biologyis a collection of chapters written by leaders in the field of MMPs. The book focuses on the challenges of understanding the mechanisms substrate degradation by MMPs, as well as how these enzymes are able to degrade large, highly ordered substrates such as collagen. All topics addressed are considered in relation to disease progression including roles in cancer metastasis, rheumatoid arthritis and other inflammatory diseases. The text first provides an overview of MMPs, focusing on the history, the development and failures of small molecule inhibitors in clinical trials, and work with TIMPS, the endogenous inhibitors of MMPs. These introductory chapters establish the foundation for later discussion of the recent progress on the design of different types of inhibitors, including novel antibody based therapeutics. The following section emphasizes research using novel methods to further the study of the MMPs. The third and final section focuses on in vivo research, particularly with respect to cancer models, degradation of the extracellular matrix, and MMP involvement in other disease states. Written and edited by leaders in the field, Matrix Metalloproteinase Biology addresses the rapidly growth in MMP research, and will be an invaluable resource to advanced students and researchers studying cell and molecular biology.
Download or read book Arthritis Research written by Andrew P. Cope and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a compendium of data pertinent to the methods and protocols that have contributed to recent advances in molecular medicine in general, but to the molecular basis of rheumatic disease in particular. These volumes details novel technologies, some of which are still evolving and whose impacts are yet to be determined. Leaders in the field contribute to cover exciting and cutting edge topics. This compendium will be a valuable tool.
Download or read book Matrix Metalloproteinases in Health and Disease written by Raffaele Serra and published by . This book was released on 2020-09-11 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: Matrix metalloproteinases (MMPs) are members of an enzyme family and are critical for maintaining tissue allostasis. MMPs can catalyze normal turnover of the extracellular matrix (ECM) together with other metalloproteinases such as ADAM (a disintegrin and metalloproteinase) and ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) families. MMP activity is also regulated by a group of endogenous proteins called tissue inhibitor of metalloproteinases (TIMPs). All these proteins have a pivotal role involving ECM remodelling in normal physiological processes such as wound healing, embryogenesis, angiogenesis, bone remodelling, immunity, and the female reproductive cycle. An imbalance in the expression or activity of MMPs can also have important consequences in diseases such as cancer, cardiovascular disease, peripheral vascular disease, chronic leg ulcers, and multiple sclerosis. In recent years, MMPs have been found to play an important role in the field of precision medicine, as they may serve as biomarkers that may predict an individual's disease predisposition, state, or progression. MMPs are also thought to be a sensible target for molecular therapy. The aim of this Special Issue is to explore the most recent findings in this field that may have an impact in healthcare systems.
Download or read book Heart Failure A Companion to Braunwald s Heart Disease E book written by Douglas L. Mann and published by Elsevier Health Sciences. This book was released on 2010-11-11 with total page 923 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dr. Douglas L. Mann, one of the foremost experts in the field, presents the 2nd Edition of Heart Failure: A Companion to Braunwald’s Heart Disease. This completely reworked edition covers the scientific and clinical guidance you need to effectively manage your patients and captures the dramatic advances made in the field over the last five years. Now in full color, this edition features eleven new chapters, including advanced cardiac imaging techniques, use of biomarkers, cell-based therapies and tissue engineering, device therapies, and much more. Consult this title on your favorite e-reader, conduct rapid searches, and adjust font sizes for optimal readability. Compatible with Kindle®, nook®, and other popular devices. Use this Braunwald’s companion as the definitive source to prepare for the ABIM’s new Heart Failure board exam. Access the fully searchable contents of the book online at Expert Consult. This edition includes 67 new authors, who are experts in the field of heart failure Stay on the cutting edge with new chapters on: The latest practice guidelines for medical and device therapy Hemodynamic assessment of heart failure Contemporary medical therapy for heart failure patients with reduced and preserved ejection fraction Biomarkers in heart failure Pulmonary hypertension Management of co-morbidities in heart failure Mechanical cardiac support devices Get up to speed with the latest clinical trials, as well as how they have influenced current practice guidelines Explore what’s changing in key areas such as basic mechanisms of heart failure, genetic screening, cell and gene therapies, pulmonary hypertension, heart failure prevention, co-morbid conditions, telemedicine/remote monitoring, and palliative care
Download or read book Cell Surface Proteases written by and published by Elsevier. This book was released on 2003-05-03 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Surface Proteases provides a comprehensive overview of these important enzymes that catalyze the hydrolysis of a protein as it degrades to a simpler substance. In the 1990s, an explosion of new discoveries shed light on the role of cell surface proteases and extended it beyond degradation of extracellular matrix components to include its influence on growth factors, cell signaling, and other cellular events. This volume unites the scientific literature from across disciplines and teases out unified themes of interactions between cell surface proteases and interconnecting cell surface-related systems -- including integrins and other adhesion molecules. Scientists and students involved in developmental biology, cell biology and disease processes will find this an indispensable resource. * Provides an overview of the entire field of cell surface proteases in a single volume * Presents major issues and astonishing discoveries at the forefront of modern developmental biology and developmental medicine * A thematic volume in the longest-running forum for contemporary issues in developmental biology with over 30 years of coverage
Download or read book Heart Failure E Book written by G. Michael Felker and published by Elsevier Health Sciences. This book was released on 2014-12-18 with total page 787 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lead editor of Braunwald's Heart Disease, Dr. Douglas L. Mann, and nationally and internationally recognized heart failure expert Dr. G. Michael Felker, bring you the latest, definitive state-of-the art information on heart failure in this outstanding Braunwald's companion volume. Heart Failure, 3rd Edition keeps you current with recent developments in the field, improved patient management strategies, and new drug therapies and implantable devices that will make a difference in your patients' lives and your practice.
Download or read book Handbook of Proteolytic Enzymes written by Alan J. Barrett and published by Academic Press. This book was released on 2012-10-30 with total page 4097 pages. Available in PDF, EPUB and Kindle. Book excerpt: Extensively revised and updated, the new edition of the highly regarded Handbook of Proteolytic Enzymes is an essential reference for biochemists, biotechnologists and molecular biologists. Edited by world-renowned experts in the field, this comprehensive work provides detailed information on all known proteolytic enzymes to date. This two-volume set unveils new developments on proteolytic enzymes which are being investigatedin pharmaceutical research for such diseases as HIV, Hepatitis C, and the common cold. Volume I covers aspartic and metallo petidases while Volume II examines peptidases of cysteine, serine, threonine and unknown catalytic type. A CD-ROM accompanies the book containing fully searchable text, specialised scissile bond searches, 3-D color structures and much more. The only comprehensive book on proteolytic enzymes Includes 671 chapters, each written by experts in their field, on proteolytic enzymes from all groups of living organisms and the viruses, including those that are currently major targets of pharmaceutical research Accompanying CD-ROM provides fully searchable text, 2D structures of peptidases in color and links directly to PubMed and MEROPS databases Each chapter describes in detail the enzyme name, its history, activity and specificity, structural chemistry, preparation, biological aspects and distinguishing features Over 1000 peptidases included
