Download or read book Lipases and Phospholipases written by Georgina Sandoval and published by Humana. This book was released on 2019-12-10 with total page 437 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases. After an overview, the book delves into activity screening and expression, optimization of the biocatalyst production and performances, and applications of lipases, phospholipases, and esterases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Lipases and Phospholipases: Methods and Protocols, Second Editionserves as an updated reference book for the large scientific community, both seasoned and novice, working with lipases, phospholipases, and related enzymes.
Download or read book Lipases written by Paul Woolley and published by Cambridge University Press. This book was released on 2012-01-26 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This 1994 book provides a fascinating account of the fast-moving field of lipase research. The contributions, written by active research workers, summarise developments in the field and give access to recent literature. It covers both the lipases proper (triglyceride lipases and the phospholipases. It gives a comprehensive picture of the state of knowledge of these enzymes, with a strong bias towards the fields that are attracting the greatest attention: their detailed molecular structure, their mechanism of action, their position in the evolution of enzymes, and their application both in the laboratory and industry. The book will continue to be of interest to those working in universities, in research institutes and in companies specialising in biotechnology. The book will also be a useful reference book for postgraduate students entering this field of research.
Download or read book Lipase written by B.K. Konwar and published by CRC Press. This book was released on 2018-05-23 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial lipases are industrially important and have gained attention due to their stability, selectivity, and broad substrate specificity. Lipases are used as medicine, and they also aid in indigestion, heartburn, allergy to gluten in wheat products (celiac disease), Crohn’s disease, and cystic fibrosis. This volume considers the industrial demand for new sources of lipases with different catalytic characteristics that stimulate the isolation, growth, and development of new microbial strains. The volume narrates the challenging metagenomic approach with the isolation of the lipase gene, its cloning into Escherichia coli, culture of the recombinant bacteria, and extraction and assessment of the lipase enzyme. Lipase-producing bacteria are available in different habitats, such as industrial wastes, vegetable oil processing factories, dairy plants, and soils contaminated with oil and oil seeds, among others. This volume is the effort of the authors to document the scientific findings carried out over the last eight years in the area of un-culturable soil microorganisms. The book presents the physic-chemical features of lipases and their specific applications in different commercial industries. The in-depth study looks at metagenomics for lipases from all angles and provides a truly informative resource. It describes the biochemical characterization of lipase enzymes with the high activity in the presence of 1% tributyrin. A wide review has been presented in the book on lipase enzymes purified from a large collection of microbes present in soil, seawater, waste-dumping sites, animal systems (including human beings), and the atmosphere. Stability of enzymes over changing environments of the industry is indeed a big issue, and the book deals at length with the changing temperatures and pH and metal ion concentrations.
Download or read book Industrial Enzymes written by Julio Polaina and published by Springer Science & Business Media. This book was released on 2007-05-16 with total page 629 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent developments in genetic engineering and protein chemistry are bringing ever more powerful means of analysis to bear on the study of enzyme structure. This volume reviews the most important types of industrial enzymes. In a balanced manner it covers three interrelated aspects of paramount importance for enzyme performance: three-dimensional protein structure, physicochemical and catalytic properties, and the range of both classical and novel applications.
Download or read book New Lipases and Proteases written by Abu Bakar Salleh and published by Nova Publishers. This book was released on 2006 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymatic processing of lipids and oils is becoming an important area of research. Hydrolytic enzymes, such as lipases and proteases are being sought after as the biocatalysts. This book focuses on the search and acquisition, isolation and purification and the characterisation of these enzymes
Download or read book The Exocrine Pancreas written by Stephen Pandol and published by Morgan & Claypool Publishers. This book was released on 2011 with total page 66 pages. Available in PDF, EPUB and Kindle. Book excerpt: The secretions of the exocrine pancreas provide for digestion of a meal into components that are then available for processing and absorption by the intestinal epithelium. Without the exocrine pancreas, malabsorption and malnutrition result. This chapter describes the cellular participants responsible for the secretion of digestive enzymes and fluid that in combination provide a pancreatic secretion that accomplishes the digestive functions of the gland. Key cellular participants, the acinar cell and the duct cell, are responsible for digestive enzyme and fluid secretion, respectively, of the exocrine pancreas. This chapter describes the neurohumoral pathways that mediate the pancreatic response to a meal as well as details of the cellular mechanisms that are necessary for the organ responses, including protein synthesis and transport and ion transports, and the regulation of these responses by intracellular signaling systems. Examples of pancreatic diseases resulting from dysfunction in cellular mechanisms provide emphasis of the importance of the normal physiologic mechanisms.
Download or read book Lipases written by Hannes Broeken and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this collection, the authors discuss endothelial lipase (EL), a recently identified member of the lipase gene family which exhibits a high degree of sequence similarity to lipoprotein lipase and hepatic lipase. The opening chapter summarizes the recent findings with regard to the molecular structure and biological mechanisms of EL, factors affecting the EL expression and activity as well as its role in some inflammatory disorders. Additionally, enzyme lipase is discussed; specifically its wide range of applications in the food, cosmetic, pharmaceutical as well as oleochemical industries. The capability of enzyme lipase to catalyse reactions with higher efficiency and stability, their chemo-, regio- and enantioselectivity properties, as well as their eco-friendly characteristics resulted in their high demand worldwide. Following this, the use of enzyme immobilization as an efficient strategy to overcome certain drawbacks is analyzed. Immobilization of lipases enhances properties such as thermostability and activity, facilitates separation of products, and provides more flexibility with enzyme/substrate contact by using various reactor configurations. This chapter will also highlight benefits of immobilization in lipase stabilization with respect to other employed methods such as chemical modification and recombinant DNA technology. In the concluding study, three commercially available lipases, namely Lipase PS-CI and Lipase PS-DI from Pseudomonas cepacia (non-specific lipase), and Lipozyme RM-IM from Rhizomucor miehei (1,3-regioselective lipase) were screened for their effect on catalyzing the acidolysis of tricaprylin with ALA. The effect of substrate molar ratio, temperature and time of reaction on incorporation of ALA were investigated and optimized by response surface methodology. The study reveals the potential of lipase-catalyzed acidolysis of palm oil with ALA as a suitable alternative to improve the nutritional properties and health-beneficial effects of edible oils.
Download or read book Lipases and Phospholipases in Drug Development written by Günter Müller and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipases and Phospholipases are key control elements in mammalian metabolism. They share many common features that set them apart from other metabolic enzyme classes, most importantly their association with biological membranes. Their potential as drug targets for the treatment of metabolic diseases is widely recognized, and the first lipase inhibitor drugs have been successfully introduced. Providing drug developers with a firm foundation for lipase-centered drug design, the editors of this volume have assembled experts from different scientific disciplines to create a comprehensive handbook for all pharmaceutical chemists, biochemists and physiologists working with lipases. The authors examine fundamental aspects of lipase function in vitro and in vivo, explaining how this knowledge may be used to develop lipase assays. They also treat the physiological roles of lipases in normal and disordered metabolism, as well as strategies to target lipases for the treatment of diabetes, obesity and related disorders. Additional topics include the application of phospholipases for liposome-based drug delivery and their use as diagnostic tools.
Download or read book Esterases Lipases and Phospholipases written by M.I. Mackness and published by Springer Science & Business Media. This book was released on 2013-11-21 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: The NATO-Advanced Research Workshop "Esterases, Lipases and Phospholipases: From Structure to Clinical Significance" was held at the University of Bordeaux II, France from 22nd- 24th September 1993 under the Directorship of Professor Michel Clerc of the University of Bordeaux II. The meeting was organised by Hugues Chap (INSERM U 326, Toulouse, France), Georges Ferard (University of Strasbourg, France), Wolfgang Junge (University of Kiel, Germany) and Michael Mackness (University of Manchester, UK). In recent years it has become increasingly apparent that hydrolytic enzymes of the esterase, lipase and phospholipase type play central roles in the pathophysiology of many human diseases. The purpose of this NATO-ARW was to bring together experts (both clinical and scientific) in all three interrelated fields to review the current basic and clinical position and discuss future developments particularly with respect to future research aimed at determining the basic biochemical lesion involving hydrolytic enzymes involved in human disease and the use of these enzymes in diagnosis. As well as formal lectures from established researchers, the meeting also involved a number of lively round-table discussions on future developments and presentations from younger research workers, all of which are recorded in this Proceedings and which contribute to the success of the meeting.
Download or read book Lipase and Phospholipase Protocols written by Mark Doolittle and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: The lipases and phospholipases represent a diverse group of enzymes that are expressed in animals, plants, fungi, and bacteria. Their ubiquitous distribution among all species is a testament to the essential roles played by these enzymes in lipid storage, mobilization and metabolism, membrane homeostasis and remodeling, endocrine and immune functions, and signal tra- duction. In humans, lipases and phospholipases are also thought to contribute to complex diseases, such as atherosclerosis, obesity, arthritis, and cancer, as well as to single gene defects, such as Wolman's disease and Type I hyperlipoproteinemia. Enzymatically, the lipases are unique, since they hydrolyze substrates that are either insoluble, or only partly soluble, in aq- ous solvents; thus, enzyme catalysis takes place at a lipid-water interface. The interface comprises at least two, and often more, discrete bulk and s- face phases, in which the enzyme, substrate, and products oflipolysis disperse among these phases based on their physical properties. Furthermore, the d- tribution of these components changes continuously as lipolysis proceeds. Thus, the lipases and phospholipases are fundamentally different from any other enzyme because of the physical complexity of the environment in which catalysis occurs.
Download or read book Biotechnological Applications of Cold Adapted Organisms written by Rosa Margesin and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: How can industry profit from the biochemical tricks of cold-adapted organisms? This book covers a range of aspects in this fascinating field, from genetic tools to environmental biotechnology.
Download or read book Lipase written by B.K. Konwar and published by CRC Press. This book was released on 2018-05-23 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial lipases are industrially important and have gained attention due to their stability, selectivity, and broad substrate specificity. Lipases are used as medicine, and they also aid in indigestion, heartburn, allergy to gluten in wheat products (celiac disease), Crohn’s disease, and cystic fibrosis. This volume considers the industrial demand for new sources of lipases with different catalytic characteristics that stimulate the isolation, growth, and development of new microbial strains. The volume narrates the challenging metagenomic approach with the isolation of the lipase gene, its cloning into Escherichia coli, culture of the recombinant bacteria, and extraction and assessment of the lipase enzyme. Lipase-producing bacteria are available in different habitats, such as industrial wastes, vegetable oil processing factories, dairy plants, and soils contaminated with oil and oil seeds, among others. This volume is the effort of the authors to document the scientific findings carried out over the last eight years in the area of un-culturable soil microorganisms. The book presents the physic-chemical features of lipases and their specific applications in different commercial industries. The in-depth study looks at metagenomics for lipases from all angles and provides a truly informative resource. It describes the biochemical characterization of lipase enzymes with the high activity in the presence of 1% tributyrin. A wide review has been presented in the book on lipase enzymes purified from a large collection of microbes present in soil, seawater, waste-dumping sites, animal systems (including human beings), and the atmosphere. Stability of enzymes over changing environments of the industry is indeed a big issue, and the book deals at length with the changing temperatures and pH and metal ion concentrations.
Download or read book Hydrolases in Organic Synthesis written by Uwe Theo Bornscheuer and published by John Wiley & Sons. This book was released on 2006-05-12 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: From reviews to the first edtion: "Bornscheuer and Kazlauskas have set out, and succeeded, in producing a definitive manual on hydrolytic enzymes (especially lipases, esterases, and proteases) for organic chemists. This is quite simply the best book of its type and can be unreservedly recommended to organic chemists who have an interest in using hydrolytic enzymes in synthesis." (Nicholas J. Turner, University of Edinburgh) "The book is an indispensable source of information on the use of hydrolases in organic synthesis. The subject matter is very well set out, and the chapters are clearly written and presented from a critical viewpoint. Bornscheuer and Kazlauskas have succeeded admirably in describing the capabilities and limitations of the use of hydrolytic enzymes and in critically evaluating them. No library should be without the book." (Fritz Theil, WITEGA Angewandte Werkstoff-Forschung GmbH, Berlin) The second edition of this extremely successful and well-proven book presents recent developments in the use of hydrolases for organic synthesis, reflecting in particular the enormous progress made in enzyme discovery and optimization with a new chapter on "Protein Sources and Optimization of Biocatalyst Performance". The renowned authors survey the stereoselective reactions of hydrolases, especially lipases, esterases and proteases, giving researchers an overview of what has worked in the past so that they can judge how to solve their own synthetic problems. In total, the book contains over one thousand chemical structures, rounded off by some 1,800 invaluable references.
Download or read book Engineering Of with Lipases written by F.X. Malcata and published by Springer Science & Business Media. This book was released on 1996-03-31 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt: The innovative uses of lipases in a wide variety of organic syntheses and the modification of existing fats and oils have increased exponentially over the last five years, due to the increasing availability of lipases from (genetically engineered) microbial sources, coupled with their special capacity to act as catalysts at hydrophilic/hydrophobic interfaces. As a result of the structural characterisation performed during the same period, applications of these lipases can now be developed in a much more rational way. Engineering of/with Lipases presents two major topics: the design and production of lipases with desired, preselected properties, and the use of lipases for desired applications. Audience: Doctoral and post-doctoral crystallographers, biochemists, geneticists and enzyme kineticists. Food, chemical and biochemical engineers. The former will learn about the practical aims and constraints associated with industrial applications of lipases, enabling them to design lipases for specific purposes. The latter will learn how to take advantage of the structural knowledge of lipases and their metabolic genesis to better design media, processes and products in terms of biochemical and technical feasibility.
Download or read book Lingual and Gastric Lipases written by Margit Hamosh and published by CRC Press. This book was released on 2020-04-15 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 1990: This book discusses the role of fat digestion with regards to Lingual and Gastric Lipases.
Download or read book Supercritical Fluids Technology in Lipase Catalyzed Processes written by Sulaiman Al-Zuhair and published by CRC Press. This book was released on 2018-10-08 with total page 149 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes are currently used in various industries, most commonly in food, detergents, and pharmaceuticals production. Lipases are hydrolytic enzymes that demonstrate great potential as an alternative to conventional catalysts in a number of industrial applications. A complete understanding of enzymes, and their proteins structure and environmental behavior, can greatly aid in the further development of industrial applications. Supercritical Fluids Technology in Lipase Catalized Processes provides basic information about enzymes, their sources, reaction kinetics, and main industrial applications. The book focuses in lipases. their main sources, structure, and features, with an emphasis on their specificity and interfacial activity, and presents proven techniques for isolating, extracting, and purifying. Comprised of six compact chapters, this comprehensive guide introduces: Immobilization techniques and immobilized lipases that allow repeated use (which is essential from an economic point of view) Different bioreactor configurations using immobilized lipases The latest information on the available technologies in lipolytic reactions The advantages of nonaqueous media in biochemical synthesis over aqueous and solvent-free systems Material on the use of lipases in nonaqueous media to overcome the drawbacks usually encountered with the use of conventional chemical catalysts The use of supercritical fluids (SCFs) as a green alternative reaction medium Factors affecting the physical properties of lipases in this medium and, hence, their activity and stability A case study using supercritical carbon dioxide (SC-CO2) for biodiesel production Novel, cutting-edge technology, using immobilized enzymes to reduce the overall production cost Supercritical Fluids Technology in Lipase Catalized Processes outlines the main industrial applications of common enzymes and discusses relevant challenges and innovations emerging in the field.
Download or read book The Metabolism Structure and Function of Plant Lipids written by Paul K. Stumpf and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 653 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Seventh International Symposium on the Structure and Function of Plant Lipids took place at the University of California, Davis, California July 27th to August 1st, 1986. This was the first time the Symposium was held in the United States. The list of previous host cities reads, Norwich, Karlsruhe, Goteborg, Paris, Groningen, Neuchatel. The addition of Davis to this distinguished list was made by the organizers with the doubts of people who give invitations to parties - will anybody come? In fact 155 participants registered and there were 21 spouses in attendance. The scientific program was composed of nine sessions: biochemistry of isoprenoids and sterols, function of isoprenoids and sterols, structure and function of lipids, biosynthesis of complex lipids, fatty acid oxygenases and desaturases, medium and long chain fatty acids, interaction of university, government and industrial research, algal lipids, and genetics and biotechnology. In addition to these sessions of plenary lectures, there were four poster sessions in which about 140 posters were presented. All of this was packed into four days, and there was some comment about the scarcity of time to ask questions of the speakers, discuss the posters and even to eat lunch. The compression of the program was a result of the continued desire of the organizing committees to avoid concurrent sessions. The congregation of participants into a single session increases interaction and generates a feeling of unity at these symposia.
