Download or read book Laboratory Techniques in Plant Bacteriology written by Suresh G. Borkar and published by CRC Press. This book was released on 2017-12-12 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laboratory Techniques in Plant Bacteriology is ideal for scientists and students who seek a career in plant pathogenic bacteria. This book contains 41 chapters comprising practicable techniques from isolation of bacterial plant pathogens to their identification up to species and race/biotype level. It includes identification protocols of morphological, biochemical, immunological, and molecular-based techniques. This book comprises all technological aspects of plant bacteriological studies. Its content is ideal for graduate students and research scholars including bacteriological professionals or technicians. The book ultimately provides working technologies useful for controlling bacterial disease pathogens.
Download or read book A Manual of Practical Laboratory and Field Techniques in Palaeobiology written by O.R. Green and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt: The user This manual is designed for the use of geo-scientists with an interest and need in developing palaeobiological materials as a potential source of data. To meet this objective practical procedures have been formatted for use by both professional and semi professional students with an initial understanding of palaeo biological research aims as a primary source of scientific data. I have attempted to provide an explanation and understanding of practical procedures which may be required by students undertaking palaeobiological projects as part of a degree course. The layout of this manual should be particularly beneficial in the instruction and training of geotechnologists and museum preparators. Graduate students and scientists requiring an outline of a preparation procedure will also be able to use the manual as a reference from which to assess the suitability of a procedure. This manual is also intended for use by the "committed amateur". Many of the techniques described in this manual have been devised by non-palaeontologists, and developed from methods used in archaeology, zoology and botany, as well as other areas of geology. A considerable number of the methods can be undertaken by the amateur, and in the case of many of the field procedures, should be used. This will ensure that specimens and samples can be conserved in such a manner as to facilitate any later research, and not invalidate the results of subsequent geochemical analytical techniques which might be employed.
Download or read book Laboratory Techniques in Botany written by M.J. Purvis, D.C. Collier, D. Walls and published by . This book was released on 1966 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Tissue Culture written by Robert H. Smith and published by Academic Press. This book was released on 2012-12-02 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.
Download or read book General Botany Laboratory Manual written by Jerry G. Chmielewski and published by AuthorHouse. This book was released on 2013-01-21 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: The laboratory component of General Botany provides you the opportunity to view interrelationships between and among structures, to handle live or preserved material, to become familiar with the many terms we use throughout the course, and to learn how to use a microscope properly. Each of you will have your own microscope every week, no exceptions. This laboratory is fundamental, yet integral to your understanding of General Botany. The images in your manual are intended to serve as a guide while you view permanent or prepared slides. These must be viewed by each of you independently. At no time will questions be answered re where is a particular structure, etc., unless the slide is on the stage of your microscope and in focus.The content of the laboratory is rich, as is the terminology. You must come to lab prepared. You must come to lab knowing what the various terms you are about to deal with mean. There is no such thing as finishing early that simply isn't possible.In some laboratory exercises you will be asked to identify structures of an organism. For example, Examine slide 9 labeled Rhizopus sporangia w.m. and identify the mitosporangia, mitospores, columella, mitosporangiophore, and zygotes. In all likelihood you will only be able to see mitosporangia, mitospores, columella, and mitosporangiophores. If zygotes are absent in your slide you note that the population of hyphae you are examining are only reproducing asexually. These questions are written in this manner to further fortify your understanding of the organisms in question and not to trick you. Thinking about what you are viewing is not an option but a necessity!The phylogeny we have adopted in this course is a composite. No single phylogeny best reflects our collective understanding of all the organisms included in this course so we have created one that reflects modern thought and is based on both morphological and molecular data. None is any more correct or incorrect than is any other, but this is the one that we will use, and the one we deem as most acceptable.Rest assured, much still needs to be learned about the evolution of many of the groups we will study. Regardless, the course does provide you a general overview of the evolutionary biology of these various groups. This is your starting point, it is not the endpoint!
Download or read book Current Protocols Essential Laboratory Techniques written by Sean R. Gallagher and published by John Wiley & Sons. This book was released on 2012-03-19 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.
Download or read book Laboratory Protocols in Fungal Biology written by Vijai Kumar Gupta and published by Springer Science & Business Media. This book was released on 2012-12-09 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enables the reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals. Coming Soon in the Fungal Biology series: Goyal, Manoharachary / Future Challenges in Crop Protection Against Fungal Pathogens Martín, García-Estrada, Zeilinger / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites Zeilinger, Martín, García-Estrada / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2 van den Berg, Maruthachalam / Genetic Transformation Systems in Fungi Schmoll, Dattenbock / Gene Expression Systems in Fungi Dahms / Advanced Microscopy in Mycology
Download or read book Plant Chromosomes written by Kiichi Fukui and published by CRC Press. This book was released on 1996-09-26 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: Finally - a guide to cytological techniques written specifically for the plant chromosome researcher and student. Plant Chromosomes: Laboratory Methods thoroughly covers all important approaches to the study of plant chromosomes. It reviews each specific approach and describes requisite experimental techniques. These practical descriptions cover basic, standard techniques as well as the most recent research advances and state-of-the-art technologies. Plant Chromosomes: Laboratory Methods allows you to build on the knowledge of its expert authors, who have first-hand experience with the ins and outs of each approach. Through hundreds of trouble-shooting suggestions it also helps you avoid experimental pitfalls by providing invaluable tips at critical points in the experimental process. This book gives you the information you need to improve the power of your plant chromosome research - saving you time and effort in the process. No other single volume contains so much practical information on this topic.
Download or read book Methods in Plant Molecular Biology written by Mary A. Schuler and published by Academic Press. This book was released on 2012-12-02 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can also be used by the established molecular biologist who wishes to utilize the powerful techniques of recombinant DNA to explore the mysteries of the plant kingdom. - Eight basic experiments which can be used collectively or individually cover - Recombinant Cloning and Screening in E. coli; DNA Sequencing - Plant RNA Isolation and in Vitro Translations - Plant DNA Isolations and Genomic DNA Southern AnalysisChloroplast Isolation and Protein SynthesisPlant Tissue Culture and Agrobacterium Transformations - Experiments that have been student tested for three years - Blueprints for setting up gel rigs - Comprehensive course schedule outlining individual procedures to be finished in each lab segment - Course can be tailored to suit the needs of the individual instructor
Download or read book Laboratory techniques in botany written by Marion J. Purvis and published by . This book was released on 1969 with total page 439 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology Techniques written by Heather B. Miller and published by Academic Press. This book was released on 2011-10-18 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Download or read book Basic Plant Pathology Methods written by James B. Sinclair and published by CRC Press. This book was released on 1995-04-05 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Second Edition of this bestseller brings together basic plant pathology methods published in diverse and often abstract publications. The Second Edition is updated and expanded with numerous new figures, new culture media, and additional methods for working with a greater number of organisms. Methods are easy to use and eliminate the need to seek out original articles. This reference allows for easy identification of methods appropriate for specific problems and facilities. Scientific names of pathogens and some of their hosts are updated in this edition. The book also acts as a research source providing more than 1,800 literature citations. The Second Edition includes chapters on the following: Sterilization of culture apparatus and culture media Culture of pathogens with detailed techniques for 61 fungi and selected bacteria Long-term storage of plant pathogens Detection and estimation of inoculum for 28 soilborne fungal pathogens and 5 bacterial genera-15 methods for airborne inoculum and 13 methods for seedborne pathogens Establishment of disease and testing for disease resistance Work with soil microorganisms Fungicide evaluation Biological control Bright-field microscopy
Download or read book Laboratory Guide for Conducting Soil Tests and Plant Analysis written by Jr., J. Benton Jones and published by CRC Press. This book was released on 2001-06-28 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the help of this guide, you can use obtained test results to evaluate the fertility status of soils and the nutrient element status of plants for crop production purposes. It serves as an instructional manual on the techniques used to perform chemical and physical characteristic tests on soils. Laboratory Guide for Conducting Soil Tests and Pl
Download or read book Methods in Comparative Plant Ecology written by G.A. Hendry and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Comparative Plant Ecology: A laboratory manual is a sister book to the widely acclaimed Comparative Plant Ecology by Grime, Hodgson and Hunt. It contains details on some 90 critical concise diagnostic techniques by over 40 expert contributors. In one volume it provides an authoritative bench-top guide to diagnostic techniques in experimental plant ecology.
Download or read book Plant Biology Laboratory Manual written by Almuth Tschunko and published by Benjamin-Cummings Publishing Company. This book was released on 2007 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Contains 22 inquiry-based labs with minimum cost and equipment needs. The labs are designed to encourage a holistic understanding of plants-what plants do daily and through the seasons and years, as well as the plants' roles in the ecosytems. Lab investigations range from outdoor to in-lab; experimental to observational to discussion; short-term to long-term; partly to wholly student designed. The labs include learning objectives, an introduction and procedures, thought questions, and an extended assignment or investigation. Appendices cover the metric system, data presentation, and statistics (t-test).
Download or read book Plant Molecular Biology A Laboratory Manual written by Melody S. Clark and published by Springer Science & Business Media. This book was released on 2013-11-27 with total page 551 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.
Download or read book Plant Propagation Concepts and Laboratory Exercises written by Caula A. Beyl and published by CRC Press. This book was released on 2016-01-06 with total page 499 pages. Available in PDF, EPUB and Kindle. Book excerpt: Includes a DVD Containing All Figures and Supplemental Images in PowerPoint This new edition of Plant Propagation Concepts and Laboratory Exercises presents a robust view of modern plant propagation practices such as vegetable grafting and micropropagation. Along with foundation knowledge in anatomy and plant physiology, the book takes a look into the future and how cutting edge research may impact plant propagation practices. The book emphasizes the principles of plant propagation applied in both temperate and tropical environments. In addition to presenting the fundamentals, the book features protocols and practices that students can apply in both laboratory and field experiences. The book shows readers how to choose the best methods for plant propagation including proper media and containers as well as performing techniques such as budding, cutting, layering, grafting, and cloning. It also discusses how to recognize and cope with various propagation challenges. Also included are concept chapters highlighting key information, laboratory exercises, anticipated laboratory results, stimulating questions, and a DVD containing all the figures in the book as well as some supplemental images.
