Download or read book Laboratory Protocols for Mutation Detection written by Ulf Landegren and published by Oxford University Press, USA. This book was released on 1996 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: Random mutagenesis and gene knockout experiments are standard techniques to investigate the effects of specific genetic factors. In recent years, improved methods for mutation analysis have greatly simplified the study of natural mutations and sequence variants to learn more about the function of our genetic makeup, and to predict and finely diagnose disease. Accordingly, mutation analysis is assuming a rapidly increasing importance in academic, clinical, and industrial research. This collection of protocols for mutation detection techniques arose as a set of formulas shared among participants at a HUGO-sponsored workshop held in Visby in 1995, where an impressive list of originators and major users of mutation detection methods gathered to exchange experiences. The techniques included span a wide range of areas; some are currently in use, while others illustrate principles that may be of importance in future test formats.
Download or read book PCR Mutation Detection Protocols written by Bimal D.M. Theophilus and published by Humana Press. This book was released on 2010-10-26 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR). In PCR Mutation Detection Protocols, Second Edition, expert researchers bring the subject up-to-date with key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The volume also addresses key areas such as Southern blotting, accurate diagnostics with high throughput, as well as microarray systems. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which provide the often hard to find information that may mean the difference between the success and failure of the method. Authoritative and cutting-edge, PCR Mutation Detection Protocols, Second Edition aims to stimulate postgraduate scientists, researchers, and clinicians already engaged in the area and to provide an important first step for those new to this practice wanting to adopt the powerful and essential technique in their own laboratories.
Download or read book Laboratory Methods for the Detection of Mutations and Polymorphisms in DNA written by Graham R. Taylor and published by CRC Press. This book was released on 1997-01-24 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis of DNA sequence polymorphisms and mutations is of central importance in understanding biological systems. This book is devoted to the experimental analysis of DNA and presents easy-to-follow protocols. Various techniques from the simple to the highly complex are detailed in this volume, providing a wide spectrum of available methods and practical advice. The methods are described in terms of: History and background Principles and theory Equipment and reagents Protocols Troubleshooting Applications Improvements Results Comparisons with other methods Future prospects and developments This is an essential manual for researchers working in human, animal, or plant molecular genetics and is particularly valuable for hospital and commercial laboratories.
Download or read book Guide to Mutation Detection written by Human Genome Organisation and published by John Wiley & Sons. This book was released on 2005-02-03 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide to both the theory and practice of mutation detection technology, including detailed practical protocols that are derived from courses run by the Human Genome Organization (HUGO). Guide to Mutation Detection offers the only single source for assessing the molecular biological tools for mutation analysis in order to design an experiment. The first section reviews topics such as key technologies, mutation scanning, cleavage, quality control, high throughput approaches, databases, and nomenclature. The second part covers step-by-step protocols and discussion of methods such as tag-array minisequencing, electronic hybridization, pyrosequencing, fluorescent SSCP, DHPLC, array CGH, and MADGE.
Download or read book Biotechnologies for Plant Mutation Breeding written by Joanna Jankowicz-Cieslak and published by Springer. This book was released on 2016-12-08 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.
Download or read book Engineering and Analyzing Multicellular Systems written by Lianhong Sun and published by Humana. This book was released on 2016-09-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Engineering Multicellular Systems: Methods and Protocols, focuses on laboratory procedures used in recent efforts for constructing synthetic multicellular systems and their applications. In particular, constructing multicellular systems to form various microbial ecosystems has been extensively explored to examine evolution and interactions of microbial ecosystems, while co-cultures have emerged as an efficient tool to produce some complex chemical molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Engineering Multicellular Systems: Methods and Protocols provide a comprehensive laboratory protocol reference for constructing multicellular systems for various applications.
Download or read book Mutation Detection written by Richard G. H. Cotton and published by Oxford University Press. This book was released on 1998 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers the latest tried and tested protocols for a range of detection methods, from the labs of the leading researchers in the field.
Download or read book Molecular Diagnostics written by William B. Coleman and published by Springer Science & Business Media. This book was released on 2007-10-28 with total page 593 pages. Available in PDF, EPUB and Kindle. Book excerpt: Accompanying CD-ROM contains ... "a companion eBook version of Molecular diagnostics : for the clinical laboratorian, Second edition ... for downloading and use in the reader's PC or PDA."--Page 4 of cover.
Download or read book The Nucleic Acid Protocols Handbook written by Ralph Rapley and published by Springer Science & Business Media. This book was released on 2008-06-29 with total page 997 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."
Download or read book Mutation Detection written by R. G. H. Cotton and published by IRL Press. This book was released on 1998-02-05 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnbosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers the latest tried and tested protocols for a range of detection methods, from the labs of the leading researchers in the field.
Download or read book Handbook of Mutagenicity Test Procedures written by B.J. Kilbey and published by Elsevier. This book was released on 2012-12-02 with total page 876 pages. Available in PDF, EPUB and Kindle. Book excerpt: The compilation of this book was prompted by the necessity of a bench volume which could provide the necessary background information on materials, experimental design, pitfalls and dificulties, in order to perform a particular test in an acceptable way with a minimal need for additional expert help. This Second Edition updates this information, providing: - a comprehensive bench guide - methods known to be reliable - a broad spectrum of approaches - tips to avoid pitfalls when using unfamiliar techniques - data from population records - safety aspects of mutagens and carcinogens - basic statistical concepts for experiment design This `on the bench' methodological text provides the necessary information for most of the common assays for genetic damage in use. The book includes methods which have been sufficiently used and tested to make their use reliable, but also presents methods which are not widely used at present, but which might prove most useful in screening for mutagenic effects.
Download or read book Prevention of Thalassaemias and Other Haemoglobin Disorders written by Galanello Renzo and published by . This book was released on 2003 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 1 of the Prevention Book presents the principles of a programme for the prevention of the thalassaemia and other haemoglobin disorders, including a description of the various types of disorders requiring prenatal diagnosis, the strategies used for carrier screening, and a number of annexes listing upto date epidemiological and mutation data on thalassaemia. This book was written for use in combination with Volume 2, which describes many of the laboratory protocols in great detail.
Download or read book Molecular Toxicology Protocols written by Phouthone Keohavong and published by Humana. This book was released on 2020-01-28 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully revised book includes new and improved protocols to analyze toxicity at the DNA, RNA, and protein levels using genomic, epigenomic, transcriptomic, and proteomic approaches. Sections of the book address key subjects such as bioinformatics and biostatistics, toxicant-induced lung inflammation and tumors, analysis of protein and gene expression and silencing, gene promoter methylation, detection of chromosomal and genome-wide damage, analysis of DNA-adducts and surrogate gene mutations, detection and characterization of cancer gene mutation, and many other areas of study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Molecular Toxicology Protocols, Third Edition will prove to play an important role in generating a better understanding of the field of toxicology and its sub-disciplines.
Download or read book Mutagenicity Assays and Applications written by Ashutosh Kumar and published by Academic Press. This book was released on 2017-09-26 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mutagenicity: Assays and Applications presents an extensive examination of the detection, assessment and future of mutagenicity, particularly as it concerns human health and the environment. Chapters focused on specific types of mutagens or testing methods for their detection collectively explore the current state of human and environmental mutagenesis, future perspectives and regulatory needs. The test procedures for measuring mutagenicity, their advantages and limitations are described with practical and procedural detail, along with their presentation and data processing aspects. It is an essential reference covering the breadth and depth of the field of mutagenicity studies and regulation. By providing both important introductory material and practical assays and applications, this book is useful to graduate students, academic and industry researchers and regulators at various stages of their careers, leading to improved risk assessment and regulation. Presents an up-to-date and in-depth review of the current state of mutagenesis research Draws upon the combined experience and expertise of an international group of highly respected editors and chapter authors Provides an introduction to the concept of mutagenesis with particular consideration given to novel chemicals and materials
Download or read book Laboratory Protocols for Conditional Gene Targeting written by Raul M. Torres and published by Oxford University Press on Demand. This book was released on 1997 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt: Conditional gene targeting is an emerging technique for introducing mutations into the genes of laboratory mice. This collection of protocols comes from one of the world's premier laboratories for gene targeting The Institute of Genetics in Cologne and is intended for newcomers to the field as well as more experienced researchers. Topics covered include: the Cre/loxP recombination system and gene targeting in ES cells; gene targeting strategies; Cre/loxP recombination system; Gene targeting vectors consideration and use of loxP; nonselectable modifications and removal of selection marker genes; large deletions; gene replacement; chromosomal translocations; targeted integrations; conditional gene modification; LoxP-containing transgenes; conditional gene targeting; working with ES cells; ES cell transfection overview; embryonic feeder cells; ES cells handling and use; ES cell culture and transfection; ES clone picking; identification of homologous recombinants; freezing ES clones; thawing ES clones; genomic DNA preparation; cre-mediated neomycin deletion in ES cells; generation of mice preparation; mouse breeding for ES cell injection or aggregation; injection needles and holders; blastocyst preparation/injection; embryo aggregation; blastocyst transfer;
Download or read book Cancer Susceptibility written by Michelle Webb and published by Humana Press. This book was released on 2011-08-24 with total page 295 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past two decades, spectacular advances have been made in our understanding of the molecular genetics of cancer, leading to the pursuit of identifying genes that, when mutated, result in an increased susceptibility to the disease. In Cancer Susceptibility: Methods and Protocols, experts in the field bring together the most recent technological developments for identifying and screening cancer susceptibility genes. Divided into two clear sections, the book begins with gene identification, which updates and informs scientists working at identifying novel cancer susceptibility genes, while the second part deals with mutation screening technologies that aid scientists and clinicians working to translate this knowledge into the clinic. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cancer Susceptibility: Methods and Protocols is a timely collection that seeks to provide researchers with the tools to predict and combat this terrible disease.
Download or read book DNA Technology in Forensic Science written by National Research Council and published by National Academies Press. This book was released on 1992-02-01 with total page 199 pages. Available in PDF, EPUB and Kindle. Book excerpt: Matching DNA samples from crime scenes and suspects is rapidly becoming a key source of evidence for use in our justice system. DNA Technology in Forensic Science offers recommendations for resolving crucial questions that are emerging as DNA typing becomes more widespread. The volume addresses key issues: Quality and reliability in DNA typing, including the introduction of new technologies, problems of standardization, and approaches to certification. DNA typing in the courtroom, including issues of population genetics, levels of understanding among judges and juries, and admissibility. Societal issues, such as privacy of DNA data, storage of samples and data, and the rights of defendants to quality testing technology. Combining this original volume with the new update-The Evaluation of Forensic DNA Evidence-provides the complete, up-to-date picture of this highly important and visible topic. This volume offers important guidance to anyone working with this emerging law enforcement tool: policymakers, specialists in criminal law, forensic scientists, geneticists, researchers, faculty, and students.
