Download or read book Kinetic Isotope Effect Investigation of Enzyme Mechanism in Organic Solvents written by Kenneth A. H. Adams and published by . This book was released on 1990 with total page 2 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Measurement and Analysis of Kinetic Isotope Effects written by and published by Academic Press. This book was released on 2017-09-12 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experimental Analysis of Enzyme Mechanism Using Isotope Effects, Volume 596, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this comprehensive update include Measurement of enzyme binding isotope effects, Chemical ligation and isotope labeling to locate dynamic effects, Measurement of heavy enzyme isotope effects, Extracting kinetic isotope effects from a global analysis of reaction progress curves, KIE of metabolic flux and enzymes, Solvent and Primary KIE on Flavin Enzymes, and The Rapid Determination of Primary Deuterium Isotope Effects on Enzyme-Catalyzed Proton Transfer at Carbon in 50/50 HOH/DOD. Readers who are interested in applying or understanding this research will find useful methods currently used for measuring isotope effects on solution and enzyme reactions. Written by pioneers of modern isotope effect research Is the only collection of modern kinetic isotope effect methods currently available
Download or read book Enzyme Mechanism from Isotope Effects written by Paul F. Cook and published by CRC Press. This book was released on 1991-09-20 with total page 656 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isotope effects have become one of the most powerful tools available to the enzymologist for probing enzymic mechanisms. Enzyme Mechanism from Isotope Effects presents the basic theory underlying isotope effects, including the latest findings on proton tunneling and coupled atomic notions. Specific theoretical applications are emphasized in regard to the types of information that can be obtained using isotope effects. The book also examines recent theoretical treatments of the product dependence of deuterium isotope effects, multiple isotope effects and isotope effects on intermediate partitioning. Other topics include a complete discussion of methods for measuring isotope effects, including a detailed description of the use of the isotope ratio mass spectrometer to obtain isotope effects, and a review of the literature regarding mechanistic information obtained from isotope effects for individual classes of enzyme-catalyzed reactions. Enzyme Mechanism from Isotope Effects is an excellent reference source for investigators using isotope effects in their research. The book is also valuable for reference libraries and instructors teaching courses in enzyme mechanism.
Download or read book The Investigation of Organic Reactions and Their Mechanisms written by Howard Maskill and published by John Wiley & Sons. This book was released on 2008-04-15 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: A range of alternative mechanisms can usually be postulated for most organic chemical reactions, and identification of the most likely requires detailed investigation. Investigation of Organic Reactions and their Mechanisms will serve as a guide for the trained chemist who needs to characterise an organic chemical reaction and investigate its mechanism, but who is not an expert in physical organic chemistry. Such an investigation will lead to an understanding of which bonds are broken, which are made, and the order in which these processes happen. This information and knowledge of the associated kinetic and thermodynamic parameters are central to the development of safe, efficient, and profitable industrial chemical processes, and to extending the synthetic utility of new chemical reactions in chemical and pharmaceutical manufacturing, and academic environments. Written as a coherent account of the principal methods currently used in mechanistic investigations, at a level accessible to academic researchers and graduate chemists in industry, the book is highly practical in approach. The contributing authors, an international group of expert practitioners of the techniques covered, illustrate their contributions by examples from their own research and from the relevant wider chemical literature. The book covers basic aspects such as product analysis, kinetics, catalysis, and investigation of reactive intermediates. It also includes material on significant recent developments, e.g. computational chemistry, calorimetry, and electrochemistry, in addition to topics of high current industrial relevance, e.g. reactions in multiphase systems, and synthetically useful reactions involving free radicals and catalysis by organometallic compounds.
Download or read book Isotope Effects on Enzyme catalyzed Reactions written by William Wallace Cleland and published by . This book was released on 1977 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enzyme Kinetics and Regulation written by Aaren Bennett and published by Scientific e-Resources. This book was released on 2018-07-07 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: We live in the age of science-the human and numerous other living beings' genomes have been sequenced and we are beginning to understand the capacity of the metabolic machinery responsible for life on our planet. A huge number of new genes have been discovered, a significant number of these coding for enzymes of yet obscure capacity. Understanding the kinetic behavior of an enzyme provides clues to its possible physiological role. From a biotechnological perspective, knowledge of the reactant properties of an enzyme is required for the design of immobilized enzyme-based modern processes. Biotransformations are of key importance to the pharmaceutical and sustenance industries, and knowledge of the reactant properties of enzymes, essential. This book is tied in with understanding the principles of enzyme kinetics and knowing how to use mathematical models to describe the reactant capacity of an enzyme. Coverage of the material is in no way, shape or form exhaustive. There exist many books on enzyme kinetics that offer intensive, in-depth treatises of the subject. Intracellular and extracellular physiological cascades are regulated by initiation and hindrance of different enzymes involved in these pathways. Investigating and understanding the mechanism of enzyme hindrance has become the premise of development of pharmaceutical agents. Organically active regular and synthetic inhibitors have been developed and special emphasis has been placed on investigations that define their structure-work relationships in an effort to understand the inception of their natural properties. A powerful complement to the assessment of these agents is the preparation and subsequent examination of key fractional structures, deep-seated auxiliary adjustments and the corresponding unnatural enantiomers of characteristic items. We sincerely hope that this book will represent an element in the tool kit of graduate students in applied science and chemical and biochemical engineering and furthermore of undergraduate students with formal preparing in natural chemistry, biochemistry, thermodynamics and chemical reaction kinetics.
Download or read book Isotope Effects on Enzyme catalyzed Reactions written by William Wallace Cleland and published by . This book was released on 1977 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Determination of Isotope Effects to Elucidate Enzyme Mechanisms written by John Sears Blanchard and published by . This book was released on 1979 with total page 662 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enzyme Kinetics Catalysis and Control written by Daniel L. Purich and published by Elsevier. This book was released on 2010-06-16 with total page 915 pages. Available in PDF, EPUB and Kindle. Book excerpt: Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions
Download or read book Investigations of Structure and Mechanism in Organic Reactions Using Kinetic Isotope Effects written by Matthew P. Meyer and published by . This book was released on 2001 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Kinetic and Carboxyl Carbon Isotope Effect Studies on Arginine Decarboxylase written by George J. Piazza and published by . This book was released on 1979 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Elucidation of Mechanism and Transition State Structure for Enzyme and Non enzyme Catalyzed Reactions with the Aid of Novel Methods for the Measurement of Oxygen 18 Isotope Effects written by Steven Rosenberg and published by . This book was released on 1978 with total page 692 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Kinetic Isotope Effect Studies on the Catalytic Mechanisms of Aspartate Aminotransferase and Chymotrypsin written by Douglas Albert Julin and published by . This book was released on 1984 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enzymatic Reactions in Organic Media written by A. Koskinen and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: The outlook of organic synthesis has changed many times during its tractable history. The initial focus on the synthesis of substances typical of living matter, exemplified by the first examples of organic chemistry through the synthesis of urea from inorganic substances by Liebig, was accepted as the birth of organic chemistry, and thus also of organic synthesis. Although the early developments in organic synthesis closely followed the pursuit of molecules typical in nature, towards the end of the 19th century, societal pressures placed higher demands on chemical methods appropriate for the emerging age of industrialization. This led to vast amounts of information being generated through the discovery of synthetic reactions, spectroscopic techniques and reaction mechanisms. The basic organic functional group transformations were discovered and improved during the early part of this century. Reaction mechanisms were elucidated at a growing pace, and extremely powerful spectroscopic tools, such as infrared, nuclear magnetic resonance and mass spectrometry were introduced as everyday tools for a practising organic chemist. By the 1950s, many practitioners were ready to agree that almost every molecule could be syn thesized. Some difficult stereochemical problems were exceptions; for example Woodward concluded that erythromycin was a "hopelessly complex target". This frustration led to a hectic phase of development of new and increasingly more ingenious protecting group strategies and functional group transformations, and also saw the emergence of asymmetric synthesis.
Download or read book The Use of Isotope Effects as a Probe of Enzyme Reaction Mechanisms written by Paul Martin Karl Weiss and published by . This book was released on 1987 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isotope Effects In Chemistry and Biology written by Amnon Kohen and published by CRC Press. This book was released on 2005-11-01 with total page 1092 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of isotope effects has expanded exponentially in the last decade, and researchers are finding isotopes increasingly useful in their studies. Bringing literature on the subject up to date, Isotope Effects in Chemistry and Biology covers current principles, methods, and a broad range of applications of isotope effects in the physical, biolo
Download or read book The Use of Kinetic Isotope Effects in Studies of Hydrogen Transfers written by Daniel Harris Roston and published by . This book was released on 2013 with total page 115 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present dissertation seeks to deepen our understanding of hydrogen transfers and especially C-H bond activations in enzymes. Hydrogen transfers are ubiquitous in chemistry and biology and a thorough understanding of how they occur and what factors influence them will facilitate developments in biomimetic catalysis, rational drug design, and other fields. A particular difficulty with H-transfers is the importance of nuclear quantum effects to the reaction, particularly tunneling. The overall scope of the work here aims to examine how experimental kinetic isotope effects (KIEs) can be interpreted with a particular type of tunneling model, referred to as Marcus-like models, to yield a semi-quantitative picture of the physical mechanisms of H-transfers. Previous work had used this kind of model to qualitatively interpret experimental data using a combination of intuition and generalized theories. The work here examines these theories in quantitative detail, testing and calibrating our intuition in the context of several experimental systems. The first chapter of research (ch. II) focusses on the temperature dependence of primary KIEs and how these experiments can be quantitatively interpreted as a probe for certain kinds of enzyme or solvent dynamics. The subsequent chapters (ch. III-VI) focus on the use of secondary KIEs to determine the detailed structures of tunneling ready states (TRSs) and how the dynamics of H-tunneling affect those structures. These chapters focus primarily on the TRS of the enzyme alcohol dehydrogenase, but by examining an uncatalyzed analogue to that reaction (ch. VI), the work gains some insight about similarities and differences between catalyzed and uncatalyzed reactions. In summary, the work uncovers some principles of catalysis, not just the mechanism of a catalyzed reaction. The mechanism of C-H activation presented here provides an elegant solution to problems that have been vexing to accommodate within traditional models. This work constitutes some initial steps in making Marcus-like models quantitatively useful as a supplement or even replacement for traditional models of reactivity.
