Download or read book Investigation of Shadowcasting and Replica Methods of Preparing Specimens for Electron Microscopy written by and published by . This book was released on 1951 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Sample Preparation Handbook for Transmission Electron Microscopy written by Jeanne Ayache and published by Springer Science & Business Media. This book was released on 2010-06-08 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scienti c literature detailing speci c preparation steps and numerous excellent books on the preparation of b- logical thin specimens. This does not mean to imply that physical science specimen preparation is trivial. For the most part, most physical science thin specimen pre- ration protocols can be executed in a matter of a few hours using straightforward steps. Over the years, there has been a steady stream of papers written on various aspects of preparing thin specimens from bulk materials. However, aside from s- eral seminal textbooks and a series of book compilations produced by the Material Research Society in the 1990s, no recent comprehensive books on thin specimen preparation have appeared until this present work, rst in French and now in English. Everyone knows that the data needed to solve a problem quickly are more imp- tant than ever. A modern TEM laboratory with supporting SEMs, light microscopes, analytical spectrometers, computers, and specimen preparation equipment is an investment of several million US dollars. Fifty years ago, electropolishing, chemical polishing, and replication methods were the principal specimen preparation me- ods.
Download or read book Sample Preparation Handbook for Transmission Electron Microscopy written by Jeanne Ayache and published by Springer Science & Business Media. This book was released on 2010-07-03 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scienti?c literature detailing speci?c preparation steps and numerous excellent books on the preparation of b- logical thin specimens. This does not mean to imply that physical science specimen preparation is trivial. For the most part, most physical science thin specimen pre- ration protocols can be executed in a matter of a few hours using straightforward steps. Over the years, there has been a steady stream of papers written on various aspects of preparing thin specimens from bulk materials. However, aside from s- eral seminal textbooks and a series of book compilations produced by the Material Research Society in the 1990s, no recent comprehensive books on thin spe- men preparation have appeared until this present work, ?rst in French and now in English. Everyone knows that the data needed to solve a problem quickly are more imp- tant than ever. A modern TEM laboratory with supporting SEMs, light microscopes, analytical spectrometers, computers, and specimen preparation equipment is an investment of several million US dollars. Fifty years ago, electropolishing, chemical polishing, and replication methods were the principal specimen preparation me- ods.
Download or read book Principles and Techniques of Electron Microscopy written by M. A. Hayat and published by . This book was released on 1970 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: Freeze-substitution and freeze-drying; Freezing technology; The freeze-etching technique; Freeze-etching methodology; Intepretation of freeze-etching images; Negative staining; Equipment requirements; Negative staining; Summary comments on negative staining procedures; Photography of negatively stained specimens; Particle dimensions and molecular weights; Shadow casting and replication; High resolution and shadowing; Autoradiography; Conditions for quantitation; Analysis of autoradrograms; Technical considerations.
Download or read book A Technique for Easy Removal of Direct Replicas for Electron Microscopy written by WH. Bridges and published by . This book was released on 1958 with total page 6 pages. Available in PDF, EPUB and Kindle. Book excerpt: The advantages of shadowing directly on the specimen surface in the preparation of replicas for electron microscopy are twofold. First, there is no ambiguity to the interpretation-one does not have to invert the shadow direction. Second, there is no chance for artifacts due to swelling, shrinking, or straining such as are apt to develop with replicas shadowed after removal. There is, however, a serious difficulty which arises when shadowing a metal directly on a clean metal surface. The replicas are invariably bound so tightly to the specimen surface that it is necessary to etch away the specimen to release the replica. The chemical attack usually results in breaking the replica into small pieces and negates the re-replication of the specimen without repolishing and etching.
Download or read book Specimen Preparation for Transmission Electron Microscopy of Materials written by PJ Goodhew and published by Garland Science. This book was released on 2020-11-25 with total page 50 pages. Available in PDF, EPUB and Kindle. Book excerpt: Details the essential practical steps which must precede microscopy. Methods for preparing sheet or disc specimens and final thinning techniques are described with reference to practical problems. The book also covers methods for mounting specimens in the
Download or read book Biological Specimen Preparation for Transmission Electron Microscopy written by Audrey M. Glauert and published by Princeton University Press. This book was released on 2014-07-14 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements. All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs. Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor Originally published in 1999. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.
Download or read book Replica Shadowing and Freeze etching Techniques written by J. H. Martin Willison and published by . This book was released on 1980 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Specimen Preparation for Transmission Electron Microscopy of Materials written by and published by . This book was released on 1991 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electron Microscopic Specimen Preparation and Image Interpretation written by Ursula E. Wolff and published by . This book was released on 1964 with total page 78 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Specimen Preparation for Transmission Electron Microscopy of Materials written by Peter J. Goodhew and published by Garland Science. This book was released on 1984 with total page 56 pages. Available in PDF, EPUB and Kindle. Book excerpt: Details the essential practical steps which must precede microscopy. Methods for preparing sheet or disc specimens and final thinning techniques are described with reference to practical problems. The book also covers methods for mounting specimens in the
Download or read book The Principles and Practice of Electron Microscopy written by Ian M. Watt and published by Cambridge University Press. This book was released on 1997-01-30 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of this book was widely praised as an excellent introduction to electron microscopy for materials scientists, physicists, earth and biological scientists. This completely revised new edition contains expanded coverage of existing topics and much new material. The author presents the subject of electron microscopy in a readable way, open both to those inexperienced in the technique, and also to practising electron microscopists. The coverage has been brought completely up to date, whilst retaining descriptions of early classic techniques. Currently live topics such as computer control of microscopes, energy-filtered imaging, cryo- and environmental microscopy, digital imaging, and high resolution scanning and transmission microscopy are all described. The highly praised case studies of the first edition have been expanded to include some interesting new examples. This indispensable guide to electron microscopy, written by an author with thirty years practical experience, will be invaluable to new and experienced electron microscopists in any area of science and technology.
Download or read book Electron Microscopy written by Sydney S. Breese and published by Academic Press. This book was released on 2013-09-24 with total page 713 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fifth International Congress for Electron Microscopy, Volume 2: Biology focuses on the processes, methodologies, approaches, and principles involved in electron microscopy. The selection takes a look at some aspects of freeze-substitution in electron microscopy; fixatives for cytological and cytochemical studies; intramembranous localization of succinic dehydrogenase using tetranitro-blue tetrazolium; and evaluation of different methods of auto-radiography in electron microscopy. The book then examines the fixation of nuclear structures by unbuffered solutions of osmium tetroxide in slightly acid distilled water; some electron microscope observations on the contraction mechanism in vertebrate smooth muscle; arrangement of myofilaments in the oblique-striated muscles; and electron microscopic observations of cat splenic nerve fibers after fixation by freeze-drying. The text ponders on differentiation of oligodendroglia from migratory spongioblasts; morphological changes in the Purkinje-cells after orthostatic collapse; electron microscopic observations of the development of the neuroblast in the rabbit embryo; electron microscopic observations on the development of blood vessels in the nervous system of the rabbit embryo; and simple methods for nucleic acid and acid mucopolysaccharide localization in electron micrographs. The selection is a valuable source of data for researchers interested in electron microscopy.
Download or read book Symposium on Techiques for Electron Metallography written by and published by ASTM International. This book was released on with total page 151 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Metallographic Specimen Preparation written by J. McCall and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: /.letallography is much more than taking striking pictures at high magnifications or polishing and etching specimens in such a way that no scratches can be seen. Basically, metallography is the physical metallurgist's most useful and most used tool for studying metals. Although it is perhaps his oldest tool, it certainly is not likely to become obsolete. Rather, the continued demands that have been placed upon materials have required more detailed charac terizations of their microstructures and this, in turn, has re quired the metallographer to develop new techniques to make these characterizations. Not too many years ago, the metallographer had only optical microscopes with which to examine his specimens. Now he has elec tron microscopes, scanning electron microscopes, and a whole host of instruments which were unknown to him only a relatively few years ago. This has forced him to learn not only how to use these new instruments and how to interpret the information that they provide but it also has made him develop new techniques for preparing the samples for examination.
Download or read book Handbook of Sample Preparation for Scanning Electron Microscopy and X Ray Microanalysis written by Patrick Echlin and published by Springer. This book was released on 2010-11-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scanning electr on microscopy (SEM) and x-ray microanalysis can produce magnified images and in situ chemical information from virtually any type of specimen. The two instruments generally operate in a high vacuum and a very dry environment in order to produce the high energy beam of electrons needed for imaging and analysis. With a few notable exceptions, most specimens destined for study in the SEM are poor conductors and composed of beam sensitive light elements containing variable amounts of water. In the SEM, the imaging system depends on the specimen being sufficiently electrically conductive to ensure that the bulk of the incoming electrons go to ground. The formation of the image depends on collecting the different signals that are scattered as a consequence of the high energy beam interacting with the sample. Backscattered electrons and secondary electrons are generated within the primary beam-sample interactive volume and are the two principal signals used to form images. The backscattered electron coefficient ( ? ) increases with increasing atomic number of the specimen, whereas the secondary electron coefficient ( ? ) is relatively insensitive to atomic number. This fundamental diff- ence in the two signals can have an important effect on the way samples may need to be prepared. The analytical system depends on collecting the x-ray photons that are generated within the sample as a consequence of interaction with the same high energy beam of primary electrons used to produce images.
Download or read book Soil Specimen Preparation for Laboratory Testing written by and published by ASTM International. This book was released on 1976 with total page 353 pages. Available in PDF, EPUB and Kindle. Book excerpt:
