Download or read book Introduction to Experimental Biophysics written by Jay L. Nadeau and published by CRC Press. This book was released on 2017-10-10 with total page 1223 pages. Available in PDF, EPUB and Kindle. Book excerpt: Praise for the First Edition “essential reading for any physical scientist who is interested in performing biological research.” ―Contemporary Physics "an ambitious text.... Each chapter contains protocols and the conceptual reasoning behind them, which is often useful to physicists performing biological experiments for the first time." –Physics Today This fully updated and expanded text is the best starting point for any student or researcher in the physical sciences to gain firm grounding in the techniques employed in molecular biophysics and quantitative biology. It includes brand new chapters on gene expression techniques, advanced techniques in biological light microscopy (super-resolution, two-photon, and fluorescence lifetime imaging), holography, and gold nanoparticles used in medicine. The author shares invaluable practical tips and insider’s knowledge to simplify potentially confusing techniques. The reader is guided through easy-to-follow examples carried out from start to finish with practical tips and insider’s knowledge. The emphasis is on building comfort with getting hands "wet" with basic methods and finally understanding when and how to apply or adapt them to address different questions. Jay L. Nadeau is a scientific researcher and head of the Biomedical Engineering in Advanced Applications of Quantum, Oscillatory, and Nanotechnological Systems (BEAAQONS) lab at Caltech and was previously associate professor of biomedical engineering and physics at McGill University.
Download or read book Introduction to Experimental Biophysics A Laboratory Guide written by Jay L. Nadeau and published by CRC Press. This book was released on 2015-03-25 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: Easily Get Started with Biological ExperimentsIntroduction to Experimental Biophysics - A Laboratory Guide presents wet lab methods for courses in biophysics or molecular biology. A companion to the author's highly praised An Introduction to Experimental Biophysics: Biological Methods for Physical Scientists, this manual offers a flexible course pl
Download or read book Introduction to Experimental Biophysics written by Jay L. Nadeau and published by CRC Press. This book was released on 2016-04-19 with total page 658 pages. Available in PDF, EPUB and Kindle. Book excerpt: Increasing numbers of physicists, chemists, and mathematicians are moving into biology, reading literature across disciplines, and mastering novel biochemical concepts. To succeed in this transition, researchers must understand on a practical level what is experimentally feasible. The number of experimental techniques in biology is vast and often s
Download or read book Introduction to Experimental Biophysics written by Jay Nadeau and published by CRC Press. This book was released on 2011-09-21 with total page 674 pages. Available in PDF, EPUB and Kindle. Book excerpt: Increasing numbers of physicists, chemists, and mathematicians are moving into biology, reading literature across disciplines, and mastering novel biochemical concepts. To succeed in this transition, researchers must understand on a practical level what is experimentally feasible. The number of experimental techniques in biology is vast and often specific to particular subject areas; nonetheless, there are a few basic methods that provide a conceptual underpinning for broad application. Introduction to Experimental Biophysics is the ideal benchtop companion for physical scientists interested in getting their hands wet. Assuming familiarity with basic physics and the scientific method but no previous background in biology or chemistry, this book provides: A thorough description of modern experimental and analytical techniques used in biological and biophysical research Practical information and step-by-step guidance on instrumentation and experimental design Recipes for common solutions and media, lists of important reagents, and a glossary of biological terms used Developed for graduate students in biomedical engineering, physics, chemical engineering, chemistry, mathematics, and computer science, Introduction to Experimental Biophysics is an essential resource for scientists to overcoming conceptual and technical barriers to working in a biology wet lab.
Download or read book Introduction to Experimental Biophysics Set written by Jay Nadeau and published by CRC Press. This book was released on 2015-05-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This set presents wet lab methods for courses in biophysics or molecular biology. It provides a thorough description of modern experimental and analytical techniques used in biological and biophysical research. The author gives practical, step-by-step guidance on instrumentation and experimental design and includes recipes for common solutions and media, lists of important reagents, and a glossary of biological terms. The manual offers a flexible course plan that permits completion of the labs in either a full term or intensive summer course. Tested in a pedagogical setting, the experiments follow a logical progression beginning with a DNA construct. The book starts with the basics of molecular cloning: amplifying and purifying plasmid, plasmid mapping, and using restriction enzymes. Later experiments deal with more advanced, emerging techniques, such as the synthesis and characterization of quantum dots and gold nanoparticles, protein crystallization, and spectroscopic techniques.
Download or read book Biophysics for Beginners written by Helmut Schiessel and published by CRC Press. This book was released on 2013-12-20 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biophysics is a new way of looking at living matter. It uses quantitative experimental and theoretical methods to open a new window for studying and understanding life processes. This textbook gives compact introductions to the basics of the field, including molecular cell biology and statistical physics. It then presents in-depth discussions of more advanced biophysics subjects, progressing to state-of-the-art experiments and their theoretical interpretations. The book is unique by offering a general introduction to biophysics, yet at the same time restricting itself to processes that occur inside the cell nucleus and that involve biopolymers (DNA, RNA, and proteins). This allows for an accessible read for beginners and a springboard for specialists who wish to continue their study in more detail.
Download or read book Computer Simulation and Data Analysis in Molecular Biology and Biophysics written by Victor Bloomfield and published by Springer Science & Business Media. This book was released on 2009-06-05 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an introduction to two important aspects of modern bioch- istry, molecular biology, and biophysics: computer simulation and data analysis. My aim is to introduce the tools that will enable students to learn and use some f- damental methods to construct quantitative models of biological mechanisms, both deterministicandwithsomeelementsofrandomness;tolearnhowconceptsofpr- ability can help to understand important features of DNA sequences; and to apply a useful set of statistical methods to analysis of experimental data. The availability of very capable but inexpensive personal computers and software makes it possible to do such work at a much higher level, but in a much easier way, than ever before. TheExecutiveSummaryofthein?uential2003reportfromtheNationalAcademy of Sciences, “BIO 2010: Transforming Undergraduate Education for Future - search Biologists” [12], begins The interplay of the recombinant DNA, instrumentation, and digital revolutions has p- foundly transformed biological research. The con?uence of these three innovations has led to important discoveries, such as the mapping of the human genome. How biologists design, perform, and analyze experiments is changing swiftly. Biological concepts and models are becoming more quantitative, and biological research has become critically dependent on concepts and methods drawn from other scienti?c disciplines. The connections between the biological sciences and the physical sciences, mathematics, and computer science are rapidly becoming deeper and more extensive.
Download or read book Biophysics written by William Bialek and published by Princeton University Press. This book was released on 2012-12-17 with total page 653 pages. Available in PDF, EPUB and Kindle. Book excerpt: A physicist's guide to the phenomena of life Interactions between the fields of physics and biology reach back over a century, and some of the most significant developments in biology—from the discovery of DNA's structure to imaging of the human brain—have involved collaboration across this disciplinary boundary. For a new generation of physicists, the phenomena of life pose exciting challenges to physics itself, and biophysics has emerged as an important subfield of this discipline. Here, William Bialek provides the first graduate-level introduction to biophysics aimed at physics students. Bialek begins by exploring how photon counting in vision offers important lessons about the opportunities for quantitative, physics-style experiments on diverse biological phenomena. He draws from these lessons three general physical principles—the importance of noise, the need to understand the extraordinary performance of living systems without appealing to finely tuned parameters, and the critical role of the representation and flow of information in the business of life. Bialek then applies these principles to a broad range of phenomena, including the control of gene expression, perception and memory, protein folding, the mechanics of the inner ear, the dynamics of biochemical reactions, and pattern formation in developing embryos. Featuring numerous problems and exercises throughout, Biophysics emphasizes the unifying power of abstract physical principles to motivate new and novel experiments on biological systems. Covers a range of biological phenomena from the physicist's perspective Features 200 problems Draws on statistical mechanics, quantum mechanics, and related mathematical concepts Includes an annotated bibliography and detailed appendixes
Download or read book Introduction to Cellular Biophysics Volume 1 written by Armin Kargol and published by Morgan & Claypool Publishers. This book was released on 2019-03-29 with total page 117 pages. Available in PDF, EPUB and Kindle. Book excerpt: All living matter is comprised of cells, small compartments isolated from the environment by a cell membrane and filled with concentrated solutions of various organic and inorganic compounds. Some organisms are single-cell, where all life functions are performed by that cell. Others have groups of cells, or entire organs, specializing in one particular function. The survival of the entire organism depends on all of its cells and organs fulfilling their roles. While the cells are studied by different sciences, they are seen differently by biologists, chemists, or physicists. Biologists concentrate their attention on cell structure and function. What does the cell consist of? Where are its organelles? What function does each organelle fulfil? From a chemists' point of view, a cell is a complex chemical reaction chamber where various molecules are synthesized or degraded. The main question is how these, sometimes very complicated chains of reactions are controlled. Finally, from a physics standpoint, one of the main questions is the physical movement of all these molecules between organelles within the cell, as well as their exchange with the extracellular medium. The aim of this book is to look into the basic physical phenomena occurring in cells. These physical transport processes facilitate chemical reactions in the cell and that in turn leads to the biological functions necessary for the cell to satisfy its role in the mother organism. Ultimately, the goals of every cell are to stay alive and to fulfil its function as a part of a larger organ or organism. This book is an inventory of physical transport processes occurring in cells while the second volume will be a closer look at how complex biological and physiological cell phenomena result from these very basic physical processes.
Download or read book Integrative Biophysics written by Fritz-Albert Popp and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 499 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most of the specialists working in this interdisciplinary field of physics, biology, biophysics and medicine are associated with "The International Institute of Biophysics" (IIB), in Neuss, Germany, where basic research and possibilities for applications are coordinated. The growth in this field is indicated by the increase in financial support, interest from the scientific community and frequency of publications. Audience: The scientists of IIB have presented the most essential background and applications of biophotonics in these lecture notes in biophysics, based on the summer school lectures by this group. This book is devoted to questions of elementary biophysics, as well as current developments and applications. It will be of interest to graduate and postgraduate students, life scientists, and the responsible officials of industries and governments looking for non-invasive methods of investigating biological tissues.
Download or read book Quantitative Understanding of Biosystems written by Thomas M. Nordlund and published by CRC Press. This book was released on 2019-04-16 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Praise for the prior edition "The author has done a magnificent job... this book is highly recommended for introducing biophysics to the motivated and curious undergraduate student." ―Contemporary Physics "a terrific text ... will enable students to understand the significance of biological parameters through quantitative examples―a modern way of learning biophysics." ―American Journal of Physics "A superb pedagogical textbook... Full-color illustrations aid students in their understanding" ―Midwest Book Review This new edition provides a complete update to the most accessible yet thorough introduction to the physical and quantitative aspects of biological systems and processes involving macromolecules, subcellular structures, and whole cells. It includes two brand new chapters covering experimental techniques, especially atomic force microscopy, complementing the updated coverage of mathematical and computational tools. The authors have also incorporated additions to the multimedia component of video clips and animations, as well as interactive diagrams and graphs. Key Features: Illustrates biological examples with estimates and calculations of biophysical parameters. Features two brand-new chapters on experimental methods, a general overview and focused introduction to atomic force microscopy. Includes new coverage of important topics such as measures of DNA twist, images of nanoparticle assembly, and novel optical and electron nanoscopy. Provides a guide to investigating current expert biophysical research. Enhanced self-study problems and an updated glossary of terms.
Download or read book An Introduction to Environmental Biophysics written by Gaylon S. Campbell and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: From reviews of the first edition: "well organized . . . Recommended as an introductory text for undergraduates" -- AAAS Science Books and Films "well written and illustrated" -- Bulletin of the American Meteorological Society
Download or read book Biophysics written by Rodney Cotterill and published by John Wiley & Sons. This book was released on 2003-07-07 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biophysics is an evolving, multidisciplinary subject which applies physics to biological systems and promotes an understanding of their physical properties and behaviour. Biophysics: An Introduction, is a concise balanced introduction to this subject. Written in an accessible and readable style, the book takes a fresh, modern approach with the author successfully combining key concepts and theory with relevant applications and examples drawn from the field as a whole. Beginning with a brief introduction to the origins of biophysics, the book takes the reader through successive levels of complexity, from atoms to molecules, structures, systems and ultimately to the behaviour of organisms. The book also includes extensive coverage of biopolymers, biomembranes, biological energy, and nervous systems. The text not only explores basic ideas, but also discusses recent developments, such as protein folding, DNA/RNA conformations, molecular motors, optical tweezers and the biological origins of consciousness and intelligence. Biophysics: An Introduction * Is a carefully structured introduction to biological and medical physics * Provides exercises at the end of each chapter to encourage student understanding Assuming little biological or medical knowledge, this book is invaluable to undergraduate students in physics, biophysics and medical physics. The book is also useful for graduate students and researchers looking for a broad introduction to the subject.
Download or read book Introduction to Cellular Biophysics Volume 2 written by Armin Kargol and published by Morgan & Claypool Publishers. This book was released on 2019-12-13 with total page 107 pages. Available in PDF, EPUB and Kindle. Book excerpt: All living matter is comprised of cells, small compartments isolated from the environment by a cell membrane and filled with concentrated solutions of various organic and inorganic compounds. Some organisms are single-cell, where all life functions are performed by that cell. Others have groups of cells, or organs, specializing in one particular function. The survival of the entire organism depends on all of its cells and organs fulfilling their roles. While the cells are studied by different sciences, they are seen differently by biologists, chemists, or physicists. Biologists concentrate their attention on cell structure and function. What the cells consists of? Where are its organelles? What function each organelle fulfils? From a chemists’ point of view, a cell is a complex chemical reaction chamber where various molecules are synthesized or degraded. The main question is how these, sometimes very complicated chains of reactions are controlled. Finally, from a physics standpoint, some of the fundamental questions are about the physical movement of all these molecules between organelles within the cell, their exchange with the extracellular medium, as well as electrical phenomena resulting from such transport. The aim of this book is to look into the basic physical phenomena occurring in cells. These physical transport processes facilitate chemical reactions in the cell and various electrical effects, and that in turn leads to biological functions necessary for the cell to satisfy its role in the mother organism. Ultimately, the goals of every cell are to stay alive and to fulfill its function as a part of a larger organ or organism. The first volume of this book is an inventory of physical transport processes occurring in cells while this second volume provides a closer look at how complex biological and physiological cell phenomena result from these very basic physical processes.
Download or read book Muscle Biophysics written by Rassier Dilson J.E and published by Springer Science & Business Media. This book was released on 2010-09-08 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Muscle contraction has been the focus of scientific investigation for more than two centuries, and major discoveries have changed the field over the years. Early in the twentieth century, Fenn (1924, 1923) showed that the total energy liberated during a contraction (heat + work) was increased when the muscle was allowed to shorten and perform work. The result implied that chemical reactions during contractions were load-dependent. The observation underlying the “Fenn effect” was taken to a greater extent when Hill (1938) published a pivotal study showing in details the relation between heat production and the amount of muscle shortening, providing investigators with the force-velocity relation for skeletal muscles. Subsequently, two papers paved the way for the current paradigm in the field of muscle contraction. Huxley and Niedergerke (1954), and Huxley and Hanson (1954) showed that the width of the A-bands did not change during muscle stretch or activation. Contraction, previously believed to be caused by shortening of muscle filaments, was associated with sliding of the thick and thin filaments. These studies were followed by the classic paper by Huxley (1957), in which he conceptualized for the first time the cross-bridge theory; filament sliding was driven by the cyclical interactions of myosin heads (cross-bridges) with actin. The original cross-bridge theory has been revised over the years but the basic features have remained mostly intact. It now influences studies performed with molecular motors responsible for tasks as diverse as muscle contraction, cell division and vesicle transport.
Download or read book Thermal Biophysics of Membranes written by Thomas Heimburg and published by John Wiley & Sons. This book was released on 2008-02-08 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: An overview of recent experimental and theoretical developments in the field of the physics of membranes, including new insights from the past decade. The author uses classical thermal physics and physical chemistry to explain our current understanding of the membrane. He looks at domain and 'raft' formation, and discusses it in the context of thermal fluctuations that express themselves in heat capacity and elastic constants. Further topics are lipid-protein interactions, protein binding, and the effect of sterols and anesthetics. Many seemingly unrelated properties of membranes are shown to be intimately intertwined, leading for instance to a coupling between membrane state, domain formation and vesicular shape. This also applies to non-equilibrium phenomena like the propagation of density pulses during nerve activity. Also included is a discussion of the application of computer simulations on membranes. For both students and researchers of biophysics, biochemistry, physical chemistry, and soft matter physics.
Download or read book Methods in Molecular Biophysics written by Nathan R. Zaccai and published by Cambridge University Press. This book was released on 2017-05-18 with total page 710 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current techniques for studying biological macromolecules and their interactions are based on the application of physical methods, ranging from classical thermodynamics to more recently developed techniques for the detection and manipulation of single molecules. Reflecting the advances made in biophysics research over the past decade, and now including a new section on medical imaging, this new edition describes the physical methods used in modern biology. All key techniques are covered, including mass spectrometry, hydrodynamics, microscopy and imaging, diffraction and spectroscopy, electron microscopy, molecular dynamics simulations and nuclear magnetic resonance. Each method is explained in detail using examples of real-world applications. Short asides are provided throughout to ensure that explanations are accessible to life scientists, physicists and those with medical backgrounds. The book remains an unparalleled and comprehensive resource for graduate students of biophysics and medical physics in science and medical schools, as well as for research scientists looking for an introduction to techniques from across this interdisciplinary field.
