Download or read book Interactions of Organic and Biomolecules with Solid Surfaces Probed by Atomic Force Microscopy written by Kyle Wagner and published by . This book was released on 2013 with total page 171 pages. Available in PDF, EPUB and Kindle. Book excerpt: Additionally, lateral force spectroscopy was used to detect differences in the yield strength of Langmuir-Blodgett bilayer films supported on solid substrates. We were able to damage Langmuir-Blodgett bilayer films controllably by a slow increase in the normal load, resulting in complete film removal. Film damage was detectable by abrupt changes in the friction forces of the films. This procedure enabled us to demonstrate the dramatic increase in the yield strength of Langmuir-Blodgett films due to addition of a poly-ionic interlayer that acted like a glue within the films.

Download or read book Studying Biomolecular Interactions Using Atomic Force Microscopy written by Carolin Madwar and published by . This book was released on 2009 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many biological systems involve mechanical interactions and specific molecular recognition events that are essential for their function. Single molecule force spectroscopy (SMFS) is a powerful and versatile atomic force microscopy (AFM)-based technique, which allows probing such interactions at the single molecule level, with piconewton sensitivity, thereby illuminating their dynamics as well as their structural and mechanical properties. A fundamental requirement in these studies is the immobilization of biomolecules between the AFM probe and the sample surface, preferably by a long flexible molecular spacer, such as poly(ethylene glycol) (PEG). The goal of this project is to investigate the binding interactions in four distinct biomolecular systems at the single molecule level, using SMFS. A new amination strategy was used to attach a novel bifunctional PEG spacer containing amine- and thiol-reactive termini. Biomolecules under investigation were tethered to AFM tips by formation of covalent linkages to this versatile spacer. Various tests, including surface plasmon resonance and a UV-based enzyme assay, were carried out to evaluate and confirm AFM tip functionalization. SMFS of biotin/streptavidin yielded an average unbinding force of 59 pN at 4000 pN/s loading rate. SMFS of concanavalin A/mannose yielded an average unbinding force of 50 pN at loading rate of 6000 pN/s. SMFS of a de novo heterodimeric E/K coiled/coil yielded an average unbinding force of 41 pN at 7000 pN/s loading rate with a k off rate calculated to be 15.8 s-1 . SMFS of these biomolecular systems were not only successful and informative, but also provide exciting directions towards future applications.

Download or read book Quantifying Interactions of Biomolecules with Inorganic Surfaces written by Abhijeet Patra and published by Springer. This book was released on 2016-12-01 with total page 115 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis demonstrates the adaptation of existing techniques and principles towards enabling clean and precise measurements of biomolecules interacting with inorganic surfaces. In particular, it includes real-time measurement of serum proteins interacting with engineered nanomaterial. Making meaningful and unambiguous measurements has been an evolving problem in the field of biology and its various allied domains, primarily due to the complex nature of experiments and the large number of possible interferants. The subsequent quantification of interactions between biomolecules and inorganic surfaces solves pressing problems in the rapidly developing fields of lipidomics and nanomedicine.

Download or read book Molecular Manipulation with Atomic Force Microscopy written by Anne-Sophie Duwez and published by CRC Press. This book was released on 2011-12-07 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the invention of scanning probe techniques in the early 1980s, scientists can now play with single atoms, single molecules, and even single bonds. Force, dynamics, and function can now be probed at the single-molecule level. Molecular Manipulation with Atomic Force Microscopy (AFM) presents a series of topics that discuss concepts and methodologies used to manipulate and study single (bio)molecules with AFM. The first part is dedicated to the pulling of single molecules with force spectroscopy to investigate molecular interactions, mechanics, and mechanochemical processes, and the second part to the manipulation, repositioning, and targeted delivery of single molecules on substrates. Single molecule manipulation is an exciting area of research which made important breakthroughs in nanoscience and which could find potential applications in a diverse range of disciplines, including chemistry, biology, physics, material and polymer science, and engineering. New and experienced AFM researchers looking for applications beyond imaging will find a wealth of information in this informative volume.

Download or read book Study Biomolecular Interactions by Bioconjugate Chemistry and Scanning Probe Nanolithography written by Jue Wang and published by . This book was released on 2004 with total page 98 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Physical Properties and Interactions of Biomolecules written by Mélanie Favre and published by . This book was released on 2008 with total page 97 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Handbook of Surfaces and Interfaces of Materials Five Volume Set written by Hari Singh Nalwa and published by Elsevier. This book was released on 2001-10-26 with total page 1915 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook brings together, under a single cover, all aspects of the chemistry, physics, and engineering of surfaces and interfaces of materials currently studied in academic and industrial research. It covers different experimental and theoretical aspects of surfaces and interfaces, their physical properties, and spectroscopic techniques that have been applied to a wide class of inorganic, organic, polymer, and biological materials. The diversified technological areas of surface science reflect the explosion of scientific information on surfaces and interfaces of materials and their spectroscopic characterization. The large volume of experimental data on chemistry, physics, and engineering aspects of materials surfaces and interfaces remains scattered in so many different periodicals, therefore this handbook compilation is needed.The information presented in this multivolume reference draws on two decades of pioneering research on the surfaces and interfaces of materials to offer a complete perspective on the topic. These five volumes-Surface and Interface Phenomena; Surface Characterization and Properties; Nanostructures, Micelles, and Colloids; Thin Films and Layers; Biointerfaces and Applications-provide multidisciplinary review chapters and summarize the current status of the field covering important scientific and technological developments made over past decades in surfaces and interfaces of materials and spectroscopic techniques with contributions from internationally recognized experts from all over the world. Fully cross-referenced, this book has clear, precise, and wide appeal as an essential reference source long due for the scientific community. The complete reference on the topic of surfaces and interfaces of materialsThe information presented in this multivolume reference draws on two decades of pioneering researchProvides multidisciplinary review chapters and summarizes the current status of the fieldCovers important scientific and technological developments made over past decades in surfaces and interfaces of materials and spectroscopic techniquesContributions from internationally recognized experts from all over the world

Download or read book Scanning Probe Microscopy of Soft Matter written by Vladimir V. Tsukruk and published by John Wiley & Sons. This book was released on 2012-01-09 with total page 663 pages. Available in PDF, EPUB and Kindle. Book excerpt: Well-structured and adopting a pedagogical approach, this self-contained monograph covers the fundamentals of scanning probe microscopy, showing how to use the techniques for investigating physical and chemical properties on the nanoscale and how they can be used for a wide range of soft materials. It concludes with a section on the latest techniques in nanomanipulation and patterning. This first book to focus on the applications is a must-have for both newcomers and established researchers using scanning probe microscopy in soft matter research. From the contents: * Atomic Force Microscopy and Other Advanced Imaging Modes * Probing of Mechanical, Thermal Chemical and Electrical Properties * Amorphous, Poorly Ordered and Organized Polymeric Materials * Langmuir-Blodgett and Layer-by-Layer Structures * Multi-Component Polymer Systems and Fibers * Colloids and Microcapsules * Biomaterials and Biological Structures * Nanolithography with Intrusive AFM Tipand Dip-Pen Nanolithography * Microcantilever-Based Sensors

Download or read book The New Frontiers of Organic and Composite Nanotechnology written by Victor Erokhin and published by Elsevier. This book was released on 2011-10-10 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: The New Frontiers of Organic and Composite Nanotechnology is an attempt to illustrate current status of modern nanotechnology. The book is divided into 3 main sections, introduction and conclusion. The introduction describes general questions of the problem and main lines of the research activities. In the first section methods of the nanostructures construction are described. Second section is dedicated to the Structure-property relationship. Special attention is paid to the description of the most powerful experimental methods and tools used in nanotechnology, such as probe microscopies, spectroscopied, and scattering methods, including the utilization of synchrotron radiation facilities. The third section describes the applications of nanotechnology in electronics, biotechnology and diagnostics. Conclusion part presents a summary of the status of works in this area and gives some perspectives of the further development. Reference to practically all original works with essential results, that resulted in the development of nanotechnology Coherent group of well-known authors in the field of nanotechnology Book spans topics applicable for both the didactic and research

Download or read book Interaction of Atoms and Molecules with Solid Surfaces written by V. Bortolani and published by Springer. This book was released on 2013-10-16 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is considerable interest, both fundamental and technological, in the way atoms and molecules interact with solid surfaces. Thus the description of heterogeneous catalysis and other surface reactions requires a detailed understand ing of molecule-surface interactions. The primary aim of this volume is to provide fairly broad coverage of atoms and molecules in interaction with a variety of solid surfaces at a level suitable for graduate students and research workers in condensed matter physics, chemical physics, and materials science. The book is intended for experimental workers with interests in basic theory and concepts and had its origins in a Spring College held at the International Centre for Theoretical Physics, Miramare, Trieste. Valuable background reading can be found in the graduate-Ievel introduction to the physics of solid surfaces by ZangwilI(1) and in the earlier works by Garcia Moliner and F1ores(2) and Somorjai.(3) For specifically molecule-surface interac tions, additional background can be found in Rhodin and Ertl(4) and March.(S) V. Bortolani N. H. March M. P. Tosi References 1. A. Zangwill, Physics at Surfaces, Cambridge University Press, Cambridge (1988). 2. F. Garcia-Moliner and F. Flores, Introduction to the Theory of Solid Surfaces, Cambridge University Press, Cambridge (1979). 3. G. A. Somorjai, Chemistry in Two Dimensions: Surfaces, Cornell University Press, Ithaca, New York (1981). 4. T. N. Rhodin and G. Erd, The Nature of the Surface Chemical Bond, North-Holland, Amsterdam (1979). 5. N. H. March, Chemical Bonds outside Metal Surfaces, Plenum Press, New York (1986).

Download or read book Probing the Nanoscale Interaction Forces and Elastic Properties of Organic and Inorganic Materials Using Force distance F D Spectroscopy written by Abhilash Vincent and published by . This book was released on 2010 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to their therapeutic applications such as radical scavenging, MRI contrast imaging, Photoluminescence imaging, drug delivery, etc., nanoparticles (NPs) have a significant importance in bio-nanotechnology. The reason that prevents the utilizing NPs for drug delivery in medical field is mostly due to their biocompatibility issues (incompatibility can lead to toxicity and cell death). Changes in the surface conditions of NPs often lead to NP cytotoxicity. Investigating the role of NP surface properties (surface charges and surface chemistry) on their interactions with biomolecules (Cells, protein and DNA) could enhance the current understanding of NP cytotoxicity. Hence, it is highly beneficial to the nanotechnology community to bring more attention towards the enhancement of surface properties of NPs to make them more biocompatible and less toxic to biological systems. Surface functionalization of NPs using specific ligand biomolecules have shown to enhance the protein adsorption and cellular uptake through more favorable interaction pathways. Cerium oxide NPs (CNPs also known as nanoceria) are potential antioxidants in cell culture models and understanding the nature of interaction between cerium oxide NPs and biological proteins and cells are important due to their therapeutic application (especially in site specific drug delivery systems). The surface charges and surface chemistry of CNPs play a major role in protein adsorption and cellular uptake. Hence, by tuning the surface charges and by selecting proper functional molecules on the surface, CNPs exhibiting strong adhesion to biological materials can be prepared. By probing the nanoscale interaction forces acting between CNPs and protein molecules using Atomic Force Microscopy (AFM) based force-distance (F-D) spectroscopy, the mechanism of CNP-protein adsorption and CNP cellular uptake can be understood more quantitatively. The work presented in this dissertation is based on the application of AFM in studying the interaction forces as well as the mechanical properties of nanobiomaterials. The research protocol employed in the earlier part of the dissertation is specifically aimed to understand the operation of F-D spectroscopy technique. The elastic properties of thin films of silicon dioxide NPs were investigated using F-D spectroscopy in the high force regime of few 100 nN to 1 [microNewton]. Here, sol-gel derived porous nanosilica thin films of varying surface morphology, particle size and porosity were prepared through acid and base catalyzed process. AFM nanoindentation experiments were conducted on these films using the F-D spectroscopy mode and the nanoscale elastic properties of these films were evaluated. The major contribution of this dissertation is a study exploring the interaction forces acting between CNPs and transferrin proteins in picoNewton scale regime using the force-distance spectroscopy technique. This study projects the importance of obtaining appropriate surface charges and surface chemistry so that the NP can exhibit enhanced protein adsorption and NP cellular uptake.

Download or read book Microscopy and Analysis written by and published by . This book was released on 2003 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Electrical Atomic Force Microscopy for Nanoelectronics written by Umberto Celano and published by Springer. This book was released on 2019-08-01 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: The tremendous impact of electronic devices on our lives is the result of continuous improvements of the billions of nanoelectronic components inside integrated circuits (ICs). However, ultra-scaled semiconductor devices require nanometer control of the many parameters essential for their fabrication. Through the years, this created a strong alliance between microscopy techniques and IC manufacturing. This book reviews the latest progress in IC devices, with emphasis on the impact of electrical atomic force microscopy (AFM) techniques for their development. The operation principles of many techniques are introduced, and the associated metrology challenges described. Blending the expertise of industrial specialists and academic researchers, the chapters are dedicated to various AFM methods and their impact on the development of emerging nanoelectronic devices. The goal is to introduce the major electrical AFM methods, following the journey that has seen our lives changed by the advent of ubiquitous nanoelectronics devices, and has extended our capability to sense matter on a scale previously inaccessible.

Download or read book Approximate Analytical Methods for Solving Ordinary Differential Equations written by T.S.L Radhika and published by CRC Press. This book was released on 2014-10-31 with total page 584 pages. Available in PDF, EPUB and Kindle. Book excerpt: Approximate Analytical Methods for Solving Ordinary Differential Equations (ODEs) is the first book to present all of the available approximate methods for solving ODEs, eliminating the need to wade through multiple books and articles. It covers both well-established techniques and recently developed procedures, including the classical series solution method, diverse perturbation methods, pioneering asymptotic methods, and the latest homotopy methods. The book is suitable not only for mathematicians and engineers but also for biologists, physicists, and economists. It gives a complete description of the methods without going deep into rigorous mathematical aspects. Detailed examples illustrate the application of the methods to solve real-world problems. The authors introduce the classical power series method for solving differential equations before moving on to asymptotic methods. They next show how perturbation methods are used to understand physical phenomena whose mathematical formulation involves a perturbation parameter and explain how the multiple-scale technique solves problems whose solution cannot be completely described on a single timescale. They then describe the Wentzel, Kramers, and Brillown (WKB) method that helps solve both problems that oscillate rapidly and problems that have a sudden change in the behavior of the solution function at a point in the interval. The book concludes with recent nonperturbation methods that provide solutions to a much wider class of problems and recent analytical methods based on the concept of homotopy of topology.

Download or read book Nanoparticles Promises and Risks written by Mihai Lungu and published by Springer. This book was released on 2014-10-28 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt: The focus of this interdisciplinary volume is on four areas of nanoparticle research: characterization, manipulation, and potential effects on humanity and the environment. The book includes a comprehensive collection of data on industrial nanoparticle creation and the characterization of the nanoscale products of these processes. The authors describe the effects of these nanoscale structures on human health and discuss prospective implementations for detection and characterization of nanoparticles in the environment. They recommend, utilizing the most up-to-date understanding of nanotechnology, methods for limiting the negative effects of these products on the environment and human health through manipulation, sorting, and filtration.

Download or read book Probing the Nature of Biomolecular Interactions Using Atomic Force Microscopy written by Srikanth Vengasandra and published by . This book was released on 2002 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Handbook of Surfaces and Interfaces of Materials Solid thin films and layers written by Hari Singh Nalwa and published by . This book was released on 2001 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt: