Download or read book Interactions Between Saccharomyces Cerevisiae Mismatch Repair Gene Variants written by Julie Akiko Heck and published by . This book was released on 2006 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mechanisms of Initiation of DNA Mismatch Repair in Saccharomyces Cerevisiae written by Scarlet Sara Shell and published by . This book was released on 2008 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA mismatch repair (MMR) is a conserved pathway that ensures replication fidelity by repairing errors that occur during replication of the genome. In eukaryotes MMR is initiated by two heterodimeric MutS homologue (MSH) complexes, Msh2-Msh3 and Msh2-Msh6, which recognize and bind mispairs and transmit signals to downstream components that effect repair. In this work we have investigated several aspects of the mechanisms by which the MSHs locate and recognize mispairs and initiate the downstream events in MMR. We have found that the N-terminal regions (NTRs) of Msh6 and Msh3 in Saccharomyces cerevisiae are long unstructured linkers that tether the MSH complexes to the sliding clamp PCNA. A second region of the Msh6 NTR is functionally partially redundant with the PCNA interacting region, and in the absence of the entire NTR MMR is severely impaired. The NTRs of Msh3 and Msh6 can be exchanged to create functional chimeric proteins, and the Msh6 NTR is functional when fused to Msh2. Here we also describe a chimeric S. cerevisiae MSH in which the mispair-binding domain of Msh6 is replaced by that of Msh3. The chimera is functional for MMR and the mispair-binding domain confers Msh3-like mispair recognition specificities. The chimera retains Msh6-like interactions with the MutL homologues, indicating that the mispair-binding domains and the type of mispair recognized do not contribute to the specificity of subsequent interactions. These findings suggest that the mispair-binding domains of Msh3 and Msh6 are modules that confer distinct mispair specificities but communicate signals of recognition to other domains in the same fashion. Additional studies described here demonstrate that a dominant negative mutation in the ATPase domain of Msh6 is sufficient to cause cancer in mice. However, cells from these mice do not show the increased resistance to drugs that is seen in MMR knock-out cells. Experiments in S. cerevisiae demonstrate that the mutation in these mice is equivalent to a previously described yeast mutation and a mutation in a human cancer case.
Download or read book Isolation and Characterization of Separation of Function Mutations in the Saccharomyces Cerevisiae MLH1 Mismatch Repair Gene written by Sumeet Sarin and published by . This book was released on 2002 with total page 64 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Analysis of the Movement of Saccharomyces Cerevisiae Mismatch Repair Proteins on DNA written by Aaron J. Plys and published by . This book was released on 2011 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt: Replication errors that escape DNA polymerase proof-reading activity are efficientl y recognized and repaired by conserved DNA mismatch repair factors. The overall result is a drastic reduction in deletion mutations. The mechanistic details of how mismatch repair proteins execute mismatch removal have not been elucidated. The aim of my thesis is to better understand how mismatch repair factors interact with DNA in order to identify mismatch sites. My work reveals that the mismatch repair complex, MLH1-PMS1, has unique DNA diffusion characteristics facilitated by structural features of the two subunits. Through bulk assays and total internal reflectance fluorescence microscopy (TIRFM), I found that MLH1PMS1 could independently bind DNA and rapidly diffuse using the thermal energy of the system. Furthermore, MLH1-PMS1 was shown to be the first passively diffusing protein that could bypass stationary nucleosomes. In contrast, the DNA diffusion activity of the mismatch recognition complex MSH2-MSH6 was blocked by nucleosomes. The timing and nature of mismatch repair is linked with replication and is thus proposed that the differences seen for the two complexes have important implications for repair in the context of the chromatin state directly at the replication fork. Each subunit of the MLH1-PMS1 complex is composed of two defined globular domains connected by an unstructured linker arm. The linker arms of the complex are proposed to facilitate topological DNA binding and diffusion along DNA in a hopping/stepping mechanism. I found that TEV protease cleavage within the linker arms of MLH1-PMS1 disrupted DNA binding and mismatch repair in vitro and in vivo. Using a genetic mismatch repair assay I found that shortening of the linker arms in MLH1 had a drastic effect on function whereas similar changes in PMS1 had little or no effect. Purified truncated complexes were able to interact with DNA and form ternary complexes with MSH2-MSH6 at a mismatch. Future studies should focus on the diffusion characteristic for these complexes. Together, my work has important implications for understanding how mismatch repair proteins can rapidly identify their targets in a chromatin landscape.
Download or read book DNA Repair and Mutagenesis written by Errol C. Friedberg and published by American Society for Microbiology Press. This book was released on 2005-11-22 with total page 2587 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential resource for all scientists researching cellular responses to DNA damage. • Introduces important new material reflective of the major changes and developments that have occurred in the field over the last decade. • Discussed the field within a strong historical framework, and all aspects of biological responses to DNA damage are detailed. • Provides information on covering sources and consequences of DNA damage; correcting altered bases in DNA: DNA repair; DNA damage tolerance and mutagenesis; regulatory responses to DNA damage in eukaryotes; and disease states associated with defective biological responses to DNA damage.
Download or read book Genome Instability written by Marco Muzi-Falconi and published by Humana Press. This book was released on 2017-10-20 with total page 663 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents forty-two methods and protocols to analyze diverse aspects of genome instability. Chapters detail mutagenesis and repair, methods to quantify and analyze the properties of DNA double-strand breaks, profile replication, replication proteins strand-specifically, genome instability, fluorescence microscopic techniques, and genomic and proteomic approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Genome Instability: Methods and Protocols aims to provide a comprehensive resource for the discovery and analysis of the proteins and pathways that are critical for stable maintenance of the genome.
Download or read book Molecular Genetics of Recombination written by Andrés Aguilera and published by Springer Science & Business Media. This book was released on 2007-04-24 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work offers a fascinating insight into a crucial genetic process. Recombination is, quite simply, one of the most important topics in contemporary biology. This book is a totally comprehensive treatment of the subject, summarizing all existing views on the topic and at the same time putting them into context. It provides in-depth and up-to-date analysis of the chapter topics, and has been written by international experts in the field.
Download or read book Genes and Genomics written by Dilip K Arora and published by Elsevier. This book was released on 2005-10-25 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: This latest volume addresses the contemporary issues related to recombination in filamentous fungi, EST data mining, fungal intervening sequences, gene silencing, DNA damage response in filamentous fungi, cfp genes of Neurospora, developmental gene sequences, site-specific recombination, heterologous gene expression, hybridization and microarray technology to enumerate biomass. This volume also analyse the current knowledge in the area of hydrophobins and genetic regulation of carotenoid biosynthesis. Over fifty world renowned scientist from both industry and academics provided in-depth information in the field of fungal genes and genomics.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Research Awards Index written by and published by . This book was released on 1989 with total page 742 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Roles for the Saccharomyces Cerevisiae MLH1 Mismatch Repair Gene in Genome Stability and Genetic Recombination written by Juan Argueso Almeida and published by . This book was released on 2004 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book How Tobacco Smoke Causes Disease written by United States. Public Health Service. Office of the Surgeon General and published by . This book was released on 2010 with total page 728 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report considers the biological and behavioral mechanisms that may underlie the pathogenicity of tobacco smoke. Many Surgeon General's reports have considered research findings on mechanisms in assessing the biological plausibility of associations observed in epidemiologic studies. Mechanisms of disease are important because they may provide plausibility, which is one of the guideline criteria for assessing evidence on causation. This report specifically reviews the evidence on the potential mechanisms by which smoking causes diseases and considers whether a mechanism is likely to be operative in the production of human disease by tobacco smoke. This evidence is relevant to understanding how smoking causes disease, to identifying those who may be particularly susceptible, and to assessing the potential risks of tobacco products.
Download or read book Epistasis and the Evolutionary Process written by Jason B. Wolf and published by Oxford University Press, USA. This book was released on 2000 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last two decades, research into epistasis has seen explosive growth and has moved the focus of research in evolutionary genetics from a traditional additive approach. We now know the effects of genes are rarely independent, and to reach a fuller understanding of the process of evolution we need to look at gene interactions as well as gene-environment interactions. This book is an overview of non-additive evolutionary genetics, integrating all work to date on all levels of evolutionary investigation of the importance of epistasis in the evolutionary process in general. It includes a historical perspective on this emerging field, in-depth discussion of terminology, discussions of the effects of epistasis at several different levels of biological organization and combinations of theoretical and experimental approaches to analysis.
Download or read book DNA Damage and Repair written by A. Castellani and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: The First International Congress on DNA Damage and Repair was held in Rome, Italy, July 12-17, 1987. It was organized by the Italian Com mission for Nuclear Alternative Energy Sources. The subject of DNA damage and repair involves almost all the fields ofbidogical sciences. Some of the more prominent ones include carcino genesis, photobiology, radiation biology, aging, enzymology, genetics, and molecular biology. These individual fields have their own interna tional meetings and although the meetings often have sessions devoted to DNA repair, they do not bring together a wide diversity of international workers in the field to exchange ideas. The purpose of the Congress was to facilitate such an exchange among scientists representing many fields of endeavor and many countries. The 37 manuscripts in this volume, presented by the invited spea kers during the four and half days of the Congress, encompass the field of DNA damage and repair. They cover biological systems ranging from mo lecules to humans and deal with damages and repair after treatment of cells with various types of radiations, chemicals, and exogenous and en dogenous oxidative damages. The Congress and its Proceedings are dedicated to two international leaders in the field of DNA damage and repair, Alexander Hollaender of the United States and Adriano Buzzati Traverso of Italy. Hollaender, who died in December 1986, was one of the first investigators to recognize the damage to DNA was important in cell killing and mutagenesis. His early work indicated that cells could recover from radiation injury.
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 1876 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Download or read book Proceedings of the National Academy of Sciences of the United States of America written by National Academy of Sciences (U.S.) and published by . This book was released on 2006 with total page 1080 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1989 with total page 1422 pages. Available in PDF, EPUB and Kindle. Book excerpt:
