Download or read book Integrated Metabolomic and Transcriptomic Analysis of Modified Nucleosides for Biomarker Discovery in Clear Cell Renal Cell Carcinoma written by Daniel A. Mohl and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Clear cell renal cell carcinoma (ccRCC) accounts for ~75% of kidney cancers. The biallelic inactivation of the von Hippel-Lindau tumor suppressor gene (VHL) is the truncal driver mutation of most cases of ccRCC. Cancer cells are metabolically reprogrammed and excrete modified nucleosides in larger amounts due to their increased RNA turnover. Modified nucleosides occur in RNAs and cannot be recycled by salvage pathways. Their potential as biomarkers has been demonstrated for breast or pancreatic cancer. To assess their suitability as biomarkers in ccRCC, we used an established murine ccRCC model, harboring Vhl, Trp53 and Rb1 (VPR) knockouts. Cell culture media of this ccRCC model and primary murine proximal tubular epithelial cells (PECs) were investigated by HPLC coupled to triple-quadrupole mass spectrometry using multiple-reaction monitoring. VPR cell lines were significantly distinguishable from PEC cell lines and excreted higher amounts of modified nucleosides such as pseudouridine, 5-methylcytidine or 2′-O-methylcytidine. The method's reliability was confirmed in serum-starved VPR cells. RNA-sequencing revealed the upregulation of specific enzymes responsible for the formation of those modified nucleosides in the ccRCC model. These enzymes included Nsun2, Nsun5, Pus1, Pus7, Naf1 and Fbl. In this study, we identified potential biomarkers for ccRCC for validation in clinical trials
Download or read book Molecular Epidemiology written by Paul A. Schulte and published by Academic Press. This book was released on 2012-12-02 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will serve as a primer for both laboratory and field scientists who are shaping the emerging field of molecular epidemiology. Molecular epidemiology utilizes the same paradigm as traditional epidemiology but uses biological markers to identify exposure, disease or susceptibility. Schulte and Perera present the epidemiologic methods pertinent to biological markers. The book is also designed to enumerate the considerations necessary for valid field research and provide a resource on the salient and subtle features of biological indicators.
Download or read book Molecular Diagnostics Promises and Possibilities written by Mousumi Debnath and published by Springer Science & Business Media. This book was released on 2010-01-29 with total page 527 pages. Available in PDF, EPUB and Kindle. Book excerpt: A rapid development in diverse areas of molecular biology and genetic engineering resulted in emergence of variety of tools. These tools are not only applicable to basic researches being carried out world over, but also exploited for precise detection of abnormal conditions in plants, animals and human body. Although a basic researcher is well versed with few techniques used by him/her in the laboratory, they may not be well acquainted with methodologies, which can be used to work out some of their own research problems. The picture is more blurred when the molecular diagnostic tools are to be used by physicians, scientists and technicians working in diagnostic laboratories in hospitals, industry and academic institutions. Since many of them are not trained in basics of these methods, they come across several gray areas in understanding of these tools. The accurate application of molecular diagnostic tools demands in depth understanding of the methodology for precise detection of the abnormal condition of living body. To meet the requirements of a good book on molecular diagnostics of students, physicians, scientists working in agricultural, veterinary, medical and pharmaceutical sciences, it needs to expose the reader lucidly to: Give basic science behind commonly used tools in diagnostics Expose the readers to detailed applications of these tools and Make them aware the availability of such diagnostic tools The book will attract additional audience of pathologists, medical microbiologists, pharmaceutical sciences, agricultural scientists and veterinary doctors if the following topics are incorporated at appropriate places in Unit II or separately as a part of Unit-III in the book. Molecular diagnosis of diseases in agricultural crops Molecular diagnosis of veterinary diseases. Molecular epidemiology, which helps to differentiate various epidemic strains and sources of disease outbreaks. Even in different units of the same hospital, the infections could be by different strains of the same species and the information becomes valuable for infection control strategies. Drug resistance is a growing problem for bacterial, fungal and parasitic microbes and the molecular biology tools can help to detect the drug resistance genes without the cultivation and in vitro sensitivity testing. Molecular diagnostics offers faster help in the selection of the proper antibiotic for the treatment of tuberculosis, which is a major problem of the in the developing world. The conventional culture and drug sensitivity testing of tuberculosis bacilli is laborious and time consuming, whereas molecular diagnosis offers rapid drug resistant gene detection even from direct clinical samples. The same approach for HIV, malaria and many more diseases needs to be considered. Molecular diagnostics in the detection of diseases during foetal life is an upcoming area in the foetal medicine in case of genetic abnormalities and infectious like TORCH complex etc. The book will be equally useful to students, scientists and professionals working in the field of molecular diagnostics.
Download or read book Metabolomics From Fundamentals to Clinical Applications written by Alessandra Sussulini and published by Springer. This book was released on 2017-01-28 with total page 353 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive view of metabolomics, from the basic concepts, through sample preparation and analytical methodologies, to data interpretation and applications in medicine. It is the first volume to cover metabolomics clinical applications while also emphasizing analytical and statistical features. Moreover, future trends and perspectives in clinical metabolomics are also presented. For researches already experienced in metabolomics, the book will be useful as an updated definitive reference. For beginners in the field and graduate students, the book will provide detailed information about concepts and experimental aspects in metabolomics, as well as examples and perspectives of applications of this strategy to clinical questions.
Download or read book Omics Technologies and Bio engineering written by Debmalya Barh and published by Academic Press. This book was released on 2017-12-01 with total page 645 pages. Available in PDF, EPUB and Kindle. Book excerpt: Omics Technologies and Bio-Engineering: Towards Improving Quality of Life, Volume 1 is a unique reference that brings together multiple perspectives on omics research, providing in-depth analysis and insights from an international team of authors. The book delivers pivotal information that will inform and improve medical and biological research by helping readers gain more direct access to analytic data, an increased understanding on data evaluation, and a comprehensive picture on how to use omics data in molecular biology, biotechnology and human health care. Covers various aspects of biotechnology and bio-engineering using omics technologies Focuses on the latest developments in the field, including biofuel technologies Provides key insights into omics approaches in personalized and precision medicine Provides a complete picture on how one can utilize omics data in molecular biology, biotechnology and human health care
Download or read book Pathology and Genetics of Tumours of the Urinary System and Male Genital Organs written by International Agency for Research on Cancer and published by IARC. This book was released on 2004 with total page 355 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume in the WHO series on histological and genetic typing of human tumors covers tumors of the kidney, the urinary system, the prostate, the testis and paratesticular tissue and the penis. Each entity is extensively discussed with information on clinicopathological, epidemiological, immunophenotypic and genetic aspects of these diseases. This book is an authoritative, concise reference, prepared by 131 authors from 22 countries. It contains more than 800 color photographs, numerous MRIs, ultrasound images, CT scans, charts and 3000 references.This book is in the series commonly referred to as the "Blue Book" series."Pathology and Genetics of Tumors of the Urinary System and Male Genital Organs"Contributors:: Dr Lauri A. Aaltonen, Dr Ferran Algaba, Dr William C. Allsbrook Jr., Dr Isabel Alvarado-Cabrero, Dr Mahul B. Amin, Dr Pedram Argani, Dr Hans Arnholdt, Dr Alberto G. Ayala, Dr Sheldon Bastacky, Dr Louis R. Begin, Dr Athanase Billis, Dr Liliane Boccon-Gibod, Dr Stephen M. Bonsib, Dr Christer Busch, Dr Paul Cairns, Dr Liang Cheng, Dr John Cheville, Dr Carlos Cordon-Cardo, Dr Antonio L. Cubilla, Dr Ivan Damjanov, Dr Charles J. Davis, Dr Angelo M. De Marzo, Dr Louis P. Dehner, Dr Brett Delahunt, Dr Gonzague De Pinieux, Dr P. Anthony Di Sant agnese, Dr Joakim Dillner, Dr John N. Eble, Dr Diana M. Eccles, Dr Lars Egevad, Dr M.N. El-Bolkainy, Dr Jonathan I. Epstein, Dr John F. Fetsch, Dr Masakuni Furusato, Dr Thomas Gasser, Dr William L. Gerald, Dr A. Geurts Van Kessel, Dr David J. Grignon, Dr Kenneth Grigor, Dr Jay L. Grosfeld, Dr Louis Guillou Dr Seife Hailemariam, Professor Ulrike Maria Hamper, Dr Arndt Hartmann, Dr Tadashi Hasegawa, Dr Axel Heidenreich, Dr Philipp U. Heitz, Dr Burkhard Helpap, Dr Riitta Herva, Professor Ferdinand Hofstadter, Professor Simon Horenblas, Dr Peter A. Humphrey, Dr Kenneth A. Iczkowski, Dr Grete Krag Jacobsen, Dr Sonny L. Johansson, Dr Michael A. Jones, Dr Peter A. Jones, Dr George W. Kaplan, Dr Charles E. Keen, Dr Kyu Rae Kim, Dr Maija Kiuru, Dr Paul Kleihues, Dr Margaret A. Knowles, Dr Gyula Kovacs, Dr Marc Ladanyi, Dr Virpi Launonen, Dr Ivo Leuschner, Dr Howard S. Levin, Dr W. Marston Linehan, Dr Leendert H.J. Looijenga, Dr Antonio Lopez-Beltran, Dr J. Carlos Manivel, Dr Guido Martignoni, Dr Alexander Marx, Dr David G. Mcleod, Dr L. Jeffrey Medeiros, Dr Maria J. Merino, Dr Helen Michael, Dr Markku Miettinen, Dr Holger Moch, Dr Henrik Moller, Dr Rodolfo Montironi, Dr F. Kash Mostofi, Dr Hartmut P.H. Neumann, Dr Manuel Nistal, Dr Lucien Nochomovitz, Dr Esther Oliva, Dr Tim D. Oliver, Dr J. Wolter Oosterhuis, Dr Attilio Orazi, Dr Chin-Chen Pan, Dr Ricardo Paniagua, Dr David M. Parham, Dr D. Max Parkin, Dr M. Constance Parkinson, Dr Christian P. Pavlovich, Dr Elizabeth J. Perlman, Dr Paola Pisani, Dr Andrew A. Renshaw, Dr Victor E. Reuter, Dr Jae Y. Ro, Professor Mark A. Rubin, Dr H. Gil Rushton, Dr Wael A. Sakr, Dr Hemamali Samaratunga, Dr Guido Sauter, Dr Paul F. Schellhammer, Dr Bernd J. Schmitz-Drager, Dr Mark Philip Schoenberg, Dr Isabell A. Sesterhenn, Dr David Sidransky, Dr Ronald Simon, Dr Leslie H. Sobin, Dr Poul H. B. Sorensen, Dr John R. Srigley, Dr Stephan Storkel, Dr Aleksander Talerman, Dr Pheroze Tamboli, Dr Puay H. Tan, Dr Bernard Tetu, Dr Kaori Togashi, Dr Lawrence True, Dr Jerzy E. Tyczynski, Dr Thomas M. Ulbright, Dr Eva Van Den Berg, Dr Theo H. Van Der Kwast, Dr Annick Vieillefond, Dr Geo Von Krogh, Dr Thomas Wheeler, Dr Paula J. Woodward, Dr Ximing J. Yang, Dr Berton Zbar"
Download or read book Metabolic Interaction in Infection written by Ricardo Silvestre and published by Springer. This book was released on 2018-04-06 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on host–pathogen interactions at the metabolic level. It explores the metabolic requirements of the infectious agents, the microbial metabolic pathways that are dedicated to circumvent host immune mechanisms as well as the molecular mechanisms by which pathogens hijack host cell metabolism for their own benefit. Finally, it provides insights on the possible clinical and immunotherapeutic applications, as well as on the available experimental and analytical methods. The contributions break new ground in understanding the metabolic crosstalk between host and pathogen.
Download or read book Proteoform Identification written by Liangliang Sun and published by Humana. This book was released on 2023-06-18 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume discusses the latest mass spectrometry (MS)-based technologies for proteoform identification, characterization, and quantification. Some of the topics covered in this book include sample preparation, proteoform separation, proteoform gas-phase fragmentation, and bioinformatics tools for MS data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Proteoform Identification: Methods and Protocols is a valuable resource for researchers in both academia and the biopharmaceutical industry who are interested in proteoform analysis using MS.
Download or read book The Handbook of Biomarkers written by Kewal K. Jain and published by Springer Science & Business Media. This book was released on 2010-02-06 with total page 509 pages. Available in PDF, EPUB and Kindle. Book excerpt: Of the thousands of biomarkers that are currently being discovered, relatively few are being validated for further applications, and the potential of a biomarker can be quite difficult to evaluate. To aid in this imperative research, Dr. Kewal K. Jain’s Handbook of Biomarkers thoroughly describes many different types of biomarkers and their discovery using various "-omics" technologies, such as proteomics and metabolomics, along with the background information needed for the evaluation of biomarkers as well as the essential procedures for their validation and use in clinical trials. With biomarkers described first according to technologies and then according to various diseases, this detailed book features the key correlations between diseases and classifications of biomarkers, which provides the reader with a guide to sort out current and future biomarkers. Comprehensive and cutting-edge, The Handbook of Biomarkers serves as a vital guide to furthering our understanding of biomarkers, which, by facilitating the combination of therapeutics with diagnostics, promise to play an important role in the development of personalized medicine, one of the most important emerging trends in healthcare today.
Download or read book Crop Improvement written by Siti Nor Akmar Abdullah and published by Springer. This book was released on 2017-10-17 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book covers the latest development in the biosciences field covering key topics in crop improvement including ‘omic approaches to improving sustainable crop production, advancement in marker technology, strategies in genetic manipulation, crop quality and sustainability and plant microbe interaction detailing on proven technologies to address critical issue for agricultural sustainability which are beneficial for researchers and students. The book also includes aspects of preserving crops after harvest as this is a key factor in promoting sustainable crop quality in terms of addressing waste, choosing the appropriate packaging and moving crops through the food and industrial supply chain. An important strategy to overcome the challenges in providing food for the world population in a sustainable manner is through concerted efforts by crop scientists to embrace new technologies in increasing yield, quality and improving food safety while minimizing adverse environmental impact of the agricultural activities. Most of the proven molecular and genetic technologies in crop science have been tested and verified in model plants such as Arabidopsis and tomato. The technologies, when deployed on various plant species of importance for human nutrition and industrial applications, including cereals, vegetables, fruits, herbs, fibre and oil crops, face many challenges, not only due to their longer life cycle but many other physiological and environmental factors affecting yield and quality of plant products. Furthermore, major impacts on crop production due to catastrophic diseases and global climate change needs urgent and innovative solutions. Therefore a systematic approach, employing various leading-edge technologies that enable the functional elucidation of key pathway genes via ‘omics tools, genome wide association with desired phenotypes and development of cost effective and practicable molecular tools for selection, is vital. The International Conference on Crop Improvement was held to address these and other pressing issues. This volume summarizes the keynote presentations from the meeting and highlights addition discussions that are critical to crop improvement in a challenging time.
Download or read book Modern Proteomics Sample Preparation Analysis and Practical Applications written by Hamid Mirzaei and published by Springer. This book was released on 2016-12-14 with total page 525 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume serves as a proteomics reference manual, describing experimental design and execution. The book also shows a large number of examples as to what can be achieved using proteomics techniques. As a relatively young area of scientific research, the breadth and depth of the current state of the art in proteomics might not be obvious to all potential users. There are various books and review articles that cover certain aspects of proteomics but they often lack technical details. Subject specific literature also lacks the broad overviews that are needed to design an experiment in which all steps are compatible and coherent. The objective of this book was to create a proteomics manual to provide scientists who are not experts in the field with an overview of: 1. The types of samples can be analyzed by mass spectrometry for proteomics analysis. 2. Ways to convert biological or ecological samples to analytes ready for mass spectral analysis. 3. Ways to reduce the complexity of the proteome to achieve better coverage of the constituent proteins. 4. How various mass spectrometers work and different ways they can be used for proteomics analysis 5. The various platforms that are available for proteomics data analysis 6. The various applications of proteomics technologies in biological and medical sciences This book should appeal to anyone with an interest in proteomics technologies, proteomics related bioinformatics and proteomics data generation and interpretation. With the broad setup and chapters written by experts in the field, there is information that is valuable for students as well as for researchers who are looking for a hands on introduction into the strengths, weaknesses and opportunities of proteomics.
Download or read book The Diabetes Textbook written by Joel Rodriguez-Saldana and published by Springer. This book was released on 2019-06-27 with total page 1040 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diabetes has become a worldwide health problem, the global estimated prevalence approaches ten percent and the burden of this disease in terms of morbidity and mortality is unprecedented. The advances acquired through the knowledge of the mechanisms of the disease and the variety of therapeutic approaches contrast with the inability of private and public health systems in underdeveloped and even developed countries to achieve the goals of treatment. This paradox has been described in many sources: the surge of scientific advances contrast with an unprecedented amount of human suffering. Thus, a patient centered and an evidence based approach with the capacity to produce measurable clinical and economic outcomes is required. The purpose of this textbook is multiple: to offer a comprehensive resource covering all aspects of outpatient management; to address diabetes as a health problem from an epidemiological, economic and clinical perspective; to discuss the role of social determinants of health on the worldwide increase in diabetes; to highlight the challenges and obstacles in providing adequate care; and to outline a multidisciplinary approach to management in which medical visits retain their importance as part of a team comprising the patient, his or her family and a multidisciplinary group of health professionals who are able to move beyond the traditional approach of diabetes as a disease and greatly improve outcomes.
Download or read book Symbiotic Microbiomes of Coral Reefs Sponges and Corals written by Zhiyong Li and published by Springer. This book was released on 2019-06-07 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the symbiotic microbiomes of invertebrates in coral reefs, especially sponges and corals. It provides in-depth and up-to-date reviews on the microbial structure and diversity, metabolism and function, symbiosis and coevolution, environment and adaption, and bioactive potentials. Meanwhile, the future perspectives will be discussed according to the existing problems and the development trend. This book will be of particular interest to the professionals in marine ecology, marine biotechnology, as well as medicinal chemists and molecular biologists.
Download or read book Cancer Cell Lines Part 1 written by John Masters and published by Springer Science & Business Media. This book was released on 2006-04-11 with total page 295 pages. Available in PDF, EPUB and Kindle. Book excerpt: Continuous cell lines derived from human cancers are the most widely used resource in laboratory-based cancer research. The first 3 volumes of this series on Human Cell Culture are devoted to these cancer cell lines. The chapters in these first 3 volumes have a common aim. Their purpose is to address 3 questions of fundamental importance to the relevance of human cancer cell lines as model systems of each type of cancer: 1. Do the cell lines available accurately represent the clinical presentation? 2. Do the cell lines accurately represent the histopathology of the original tumors? 3. Do the cell lines accurately represent the molecular genetics of this type of cancer? The cancer cell lines available are derived, in most cases, from the more aggressive and advanced cancers. There are few cell lines derived from low grade organ-confined cancers. This gap can be filled with conditionally immortalized human cancer cell lines. We do not know why the success rate for establishing cell lines is so low for some types of cancer and so high for others. The histopathology of the tumor of origin and the extent to which the derived cell line retains the differentiated features of that tumor are critical. The concept that a single cell line derived from a tumor at a particular site is representative of tumors at that site is naïve and misleading.
Download or read book Molecular Diagnostics in Cancer Patients written by Kamla Kant Shukla and published by Springer. This book was released on 2019-03-15 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book aims to bring together a broad variety of examples of the role of pharmacogenomics in current drug development, uncovering dynamic concentration-dependent drug responses on biological systems to understand pharmacodynamics responses in human cancer where genetic lesions serve as tumor markers and provide a basis for cancer diagnosis. The book describes methods and protocols applied in molecular diagnostics. It offers pathologists and researchers providing molecular diagnostic services an array of the most recent and readily accessible reference to compare methods and techniques. Highlights include the molecular diagnosis of genetic aberrations by quantitative polymerase reaction (qPCR), sequence-specific oligonucleotide arrays, next-generation sequencing (NGS), CGH arrays-and methodologies directed at the detection of epigenetic events, high-throughput nucleic acid and protein arrays, direct sequencing and FISH-based methodologies, currently used in the diagnosis of solid tumors. The book also includes an innovative line of treatment in relation to the molecular prognosis, diagnosis and pharmacogenomics in the actual practice of clinical findings at molecular levels. The book covers the applications of numerous genetic testing methodologies; in approximately the chronological order of discovery and high-throughput diagnosis using advanced genomic approaches to identify such genes, in the search for novel drug targets and/or key determinants of drug reactions. It also promotes a wider understanding of molecular diagnostics among physicians, medical students, and scientists in academics, industry and corporate world.
Download or read book Computer Aided Drug Design written by Dev Bukhsh Singh and published by Springer Nature. This book was released on 2020-10-09 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides up-to-date information on bioinformatics tools for the discovery and development of new drug molecules. It discusses a range of computational applications, including three-dimensional modeling of protein structures, protein-ligand docking, and molecular dynamics simulation of protein-ligand complexes for identifying desirable drug candidates. It also explores computational approaches for identifying potential drug targets and for pharmacophore modeling. Moreover, it presents structure- and ligand-based drug design tools to optimize known drugs and guide the design of new molecules. The book also describes methods for identifying small-molecule binding pockets in proteins, and summarizes the databases used to explore the essential properties of drugs, drug-like small molecules and their targets. In addition, the book highlights various tools to predict the absorption, distribution, metabolism, excretion (ADME) and toxicity (T) of potential drug candidates. Lastly, it reviews in silico tools that can facilitate vaccine design and discusses their limitations.
Download or read book Marine Niche Applications in Pharmaceutical Sciences written by Neelam M Nathani and published by Springer Nature. This book was released on 2020-11-09 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers a comprehensive study of biological molecules acquired from marine organisms, which have been exploited for drug discovery with the aim to treat human diseases. Biomolecules have potential impacts on a diverse range of fields, including medical and pharmaceutical science, industrial science, biotechnology, basic research, molecular science, environmental science and climate change, etc. To understand and effectively apply medicinally important biomolecules, multidisciplinary approaches are called for. The ocean remains a rich biological resource, and the vast untapped potential of novel molecules from marine bio-resources has caught the interest of more and more researchers. These novel biological compounds have never been found in terrestrial or other ecosystems, but only in this rich niche. Advances in sampling techniques and technologies, along with increased funding for research and nature conservation, have now encouraged scientists to look deeper in the waters. Aquaculture supports both tremendous seafood production and the bulk production of marine-derived drugs. Furthermore, molecular methods are now being extensively employed to explore the untapped marine microbial diversity. With the help of molecular and biotech tools, the ability of marine organisms to produce new biosynthetic drugs can be greatly enhanced. This book provides an extensive compilation of the latest information on marine resources and their undisputedly vital role in the treatment of diverse ailments.
