Download or read book In Vitro Chondrogenic Differentiation of Human Mesenchymal Stem Cells in Collagen Gels written by Ting-yan Hui and published by . This book was released on 2007 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book In Vitro Chondrogenic Differentiation of Human Mesenchymal Stem Cells in Collagen Gels written by 許婷恩 and published by Open Dissertation Press. This book was released on 2017-01-27 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "In Vitro Chondrogenic Differentiation of Human Mesenchymal Stem Cells in Collagen Gels" by 許婷恩, Ting-yan, Hui, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled In Vitro Chondrogenic Differentiation of Human Mesenchymal Stem Cells in Collagen Gels submitted by Hui Ting Yan for the Degree of Master of Philosophy at The University of Hong Kong in August, 2007 Articular cartilage, the load-bearing tissue of the joint, has limited potential for repair and regeneration. Given a lack of satisfactory surgical solutions, cartilage tissue engineering has been suggested as a promising approach for cartilage repair. The present study demonstrated the fabrication of cartilage-like tissue-engineered constructs by chondrogenic differentiation of human mesenchymal stem cells (hMSCs) in collagen gels in vitro. Collagen-hMSC constructs were synthesized with 5 6 varying cell seeding densities (110 - 510 cells/ml) and initial collagen concentrations (0.5 - 3 mg/ml) to investigate the effects of the two parameters. The constructs were cultured in chondrogenic differentiation induction medium for 21 days and evaluated by histological, immunohistochemial, morphological, biochemical and biomechanical examinations. In addition, with inclusion of chondroitin sulfate (CS), collagen/CS-hMSC constructs were also fabricated and compared to their collagen-hMSC constructs counterparts. After 21 days of culture, chondrogenesis was evident in the collagen-hMSC constructs, as indicated by positive immunohistochemical staining for cartilage-specific extracellular matrix components (type II collagen and aggrecan). The meshwork of collagen fibers was remodeled into a highly ordered microstructure, characterized by thick and parallel collagen bundles. Higher cell seeding density and higher collagen concentration favored the chondrogenic differentiation of the cells, yielding an increased matrix production (glycosaminoglycans) and a higher mechanical strength (reduced elastic modulus) of the constructs. A biochemical analysis of matrix accumulation revealed no difference between the collagen/CS-hMSC and collagen-hMSC constructs. Further investigation showed that most of the CS added during fabrication was lost to the surrounding medium within the first 24 hours. To study the effects of inclusion of CS, further studies should be performed to develop a fabrication method which enables effective CS incorporation into the constructs. The current work presented a systematic study to understand the parameters in building a cartilage-like construct, and provided the groundwork for optimization of the biological and mechanical characteristics. The findings may contribute towards the development of tissue engineering solutions for cartilage injuries. DOI: 10.5353/th_b3955874 Subjects: Chondrogenesis Collagen Stem cells Mesenchyme Tissue engineering
Download or read book A Manual For Differentiation Of Bone Marrow derived Stem Cells To Specific Cell Types written by Kee Woei Ng and published by World Scientific Publishing Company. This book was released on 2014-02-11 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first experimental protocol book that covers the differentiation of bone marrow-derived stem cells (BMSCs) into specific cell types, targeted at the undergraduate and graduate student level. The 19 chapters deal with the differentiation methods using small molecules, cytokines and polymeric scaffolds.BMSCs are pluripotential in that they not only act as myelo-regenerative and supportive cells, but can also differentiate into almost any kind of cells in our body. In addition, when implanted in vivo, they could help repair multiple tissues such as blood vessels, heart, liver and so on.For the differentiation of BMSCs, many methods have been introduced to adjust their microenvironment (chemical and physical cues), including chemical induction methods using large or small molecules and pellet culture; mechanical stimulation induction methods using cyclic mechano-transduction or ultrasonication; cytokine-released method using scaffolds; and so on.
Download or read book Mechanical and Biological Mechanisms of Regulating Human Mesenchymal Stem Cell Differentiation written by Anne Kathryn Staples and published by . This book was released on 2006 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knowledge of how hMSCs respond to different types of mechanical loading, how this response differs from a traditional growth factor approach of inducing cellular differentiation and how their responsiveness to mechanical stimulation varies with cell differentiation stage are all critical for the successful design of tissue engineering constructs that are optimally organized for a specific mechanical function.
Download or read book Functional Tissue Engineering written by Farshid Guilak and published by Springer Science & Business Media. This book was released on 2003-07-09 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: -Softcover reprint of a successful hardcover reference (370 copies sold) -Price to be accessible to the rapidly increasing population of students and investigators in the field of tissue engineering -Chapters written by well-known researchers discuss issues in functional tissue engineering as well as provide guidelines and a summary of the current state of technology
Download or read book Regulation of Human Mesenchymal Stem Cell Differentiation by Collagen Gel Microenvironment written by Stephanie Chua and published by . This book was released on 2004 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Polymer Research written by Gennadii Efremovich Zaikov and published by Nova Publishers. This book was released on 2006 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: Polymers are substances containing a large number of structural units joined by the same type of linkage. These substances often form into a chain-like structure. Starch, cellulose, and rubber all possess polymeric properties. Today, the polymer industry has grown to be larger than the aluminium, copper and steel industries combined. Polymers already have a range of applications that far exceeds that of any other class of material available to man. Current applications extend from adhesives, coatings, foams, and packaging materials to textile and industrial fibres, elastomers, and structural plastics. Polymers are also used for most composites, electronic devices, biomedical devices, optical devices, and precursors for many newly developed high-tech ceramics. This new volume presents leading-edge research in this rapidly-changing and evolving field.
Download or read book Differentiation of Mesenchymal Stem Cells in Response to Mechanical and Chemical Factors written by Jennifer Sang-ha Park and published by . This book was released on 2006 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mesenchymal Stem Cells written by Darwin J. Prockop and published by Humana Press. This book was released on 2008-03-07 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: For over forty years, mesenchymal stem cells (MSCs) have been scrutinized and studied, garnering much attention due to their broad therapeutic efficacy. In this essential book, leaders in the field were assembled to contribute detailed methodologies for the isolation and characterization of human and rodent MSCs. Cutting edge and easy to use, this book is the perfect resource for scientists attempting to pursue this important and ever-developing field of research.
Download or read book Bone and Cartilage Regeneration written by Phuc Van Pham and published by Springer. This book was released on 2017-01-24 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: This invaluable resource discusses clinical applications with effects and side-effects of applications of stem cells in bone and cartilage regeneration. Each chapter is contributed by a pre-eminent scientist in the field and covers such topics as skeletal regeneration by mesenchymal stem cells, clinical improvement of mesenchymal stem cell injection in injured cartilage and osteoarthritis, Good manufacturing practice (GMP), minimal critera of stem cells for clinical applications, future directions of the discussed therapies and much more. Bone & Cartilage Regeneration and the other books in the Stem Cells in Clinical Applications series will be invaluable to scientists, researchers, advanced students and clinicians working in stem cells, regenerative medicine or tissue engineering.
Download or read book Three Dimensional Culture and in Vitro Chondrogenic Differentiation of Mouse Embryonic Stem Cell in Collagen Microsphere written by Chiu-Wai Yeung and published by . This book was released on 2017-01-27 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Three Dimensional Culture and in Vitro Chondrogenic Differentiation of Mouse Embryonic Stem Cell in Collagen Microsphere" by Chiu-wai, Yeung, 楊超慧, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. DOI: 10.5353/th_b4218206 Subjects: Embryonic stem cells Cell culture Chondrogenesis Mice - Cytology
Download or read book Chondrogenic Differentiation of Human Mesenchymal Stem Cells and Articular Cartilage Reconstruction written by Andrea Heymer and published by . This book was released on 2008 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization of the Time dependent Differentiation of Calvaria derived Rat Mesenchymal Stem Cells in Three dimensional Agarose Gels written by Paul Rim and published by . This book was released on 2004 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Medical and Healthcare Technologies written by Borko Furht and published by Springer Science & Business Media. This book was released on 2013-11-20 with total page 530 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book equips readers to understand a complex range of healthcare products that are used to diagnose, monitor, and treat diseases or medical conditions affecting humans. The first part of the book presents medical technologies such as medical information retrieval, tissue engineering techniques, 3D medical imaging, nanotechnology innovations in medicine, medical wireless sensor networks, and knowledge mining techniques in medicine. The second half of the book focuses on healthcare technologies including prediction hospital readmission risk, modeling e-health framework, personal Web in healthcare, security issues for medical records, and personalized services in healthcare. The contributors are leading world researchers who share their innovations, making this handbook the definitive resource on these topics. Handbook of Medical and Healthcare Technologies is intended for a wide audience including academicians, designers, developers, researchers and advanced-level students. It is also valuable for business managers, entrepreneurs, and investors within the medical and healthcare industries.
Download or read book In Vitro Studies of Equine Mesenchymal Stem Cell Chondrogenic Capacity After Treatment with Transforming Growth Factor B and Insulin like Growth Factor I written by Allison Ann Worster and published by . This book was released on 2000 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Oscillating Hydrogel Based Bioreactors for Chondrogenic Differentiation of Mesenchymal Stem Cells written by Veronica Juliet Neiman and published by . This book was released on 2010 with total page 113 pages. Available in PDF, EPUB and Kindle. Book excerpt: Harnessing the differentiative potential of stem cells for use in tissue repair could be a powerful therapy for debilitating diseases. However, one of the bottlenecks of stem cell based therapeutics and tissue engineering is inefficient and homogeneous stem cell differentiation. Various physico-chemical cues such as mechanical strain, chemical components, and soluble factors have been shown to direct stem cell differentiation. This study developed a multifunctional polymer-based artificial ECM replicating the multifunctional characteristics of native ECM to understand the physico-chemical cues present in a 3D environment. Specifically, we have developed a synthetic hydrogel that acts as a scaffold and bioreactor providing dynamic mechanical cues and structural support to cells. A heating device was used to induce ~5% volume strain by applying temperature oscillations to thermoresponsive hydrogels. Human mesenchymal stem cells (hMSCs) were encapsulated in P[MEO2MA-OEGMA-EGDA] (MO) (10 and 20% Mw PEG: 3400) and PEGDA(15% Mw PEG: 10000) hydrogels and cultured with and without TGF[Beta]-1. Fluorescent particle tracking was used to measure realtime volume strains of acellular and cellular hydrogels under temperature oscillations and verified with swelling ratios. hMSCs produced cartilaginous ECM as evidenced from histological and biochemical analysis. Realtime PCR was used to characterize the expression of various chondrogenic markers, indicating optimal chondrogenic differentiation with 1 hour stimulated PEGDA (15% PEG) hydrogels and TGF[Beta]-1. Due to static mechanical strains induced by high crosslinking density and confined heating chambers, enhanced chondrogenic differentiation was limited for all gels. Overall, this study demonstrated the potential use of polymer-based synthetic bioactuators for stem cell differentiation.
Download or read book Chondrogenic Differentiation of Human Mesenchymal Stem Cells in RADA16 I and Methylcellulose based Bioink written by Gustaf Jonsson Jonsson and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Printing and investigating cell-laden and self assembeled peptide scafolds.
