Download or read book In Vitro Characterization of the Cholesterol 7 alpha hydroxylase Gene written by Roya Bashirzadeh and published by . This book was released on 1992 with total page 102 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Cellular and Regulatory Characterization of Cholesterol 7 Alpha hydroxylase written by Emelyn R. Elderedge and published by . This book was released on 1989 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Vitamin D Receptor Regulation of Cholesterol 7 alpha hydroxylase Gene Transcription and Bile Acid Synthesis in Human Hepatocytes written by Shuxin Han and published by . This book was released on 2009 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vitamin D receptor (VDR) plays an important role in the regulation of calcium and phosphate homeostasis and bone formation. Lithocholic acid (LCA) is a potent endogenous ligand of VDR. In cholestasis, LCA levels increase in the liver and intestine. The objective of this study is to test the hypothesis that VDR plays a role in inhibiting the gene expression of cholesterol 7[alpha]-hydroxylase (CYP7A1) and bile acid synthesis in human hepatocytes. Immunoblot analysis has detected VDR proteins in the human hepatoma cell line HepG2 and primary hepatocytes. 1[alpha], 25-dihydroxy-vitamin D3 (1[alpha], 25(OH)2-VD3) or LCA acetate-activated VDR inhibited CYP7A1 mRNA expression and bile acid synthesis, whereas small interfering RNA (siRNA) to VDR completely abrogated VDR inhibition of CYP7A1 in HepG2 cells. LCA or 1[alpha], 25(OH)2-VD3 induced VDR translocation from the cytosol to the nucleus and plasma membrane in human primary hepatocytes. LCA or 1[alpha], 25(OH)2-VD3 activated a tyrosine kinase c-Src and the VDR signaling pathway, which activated c-Raf, an upstream kinase of the MAPK pathway. Kinase inhibition and in vitro kinase assays showed that VDR specifically activated extracellular signal-regulated kinase 1/2 (ERK1/2), which phosphorylated VDR, retinoid X receptor [alpha] (RXR[alpha]) and hepatocyte nuclear factor 4[alpha] (HNF4[alpha]). Mammalian two-hybrid, coimmunoprecipitation, glutathione S-transferase pull-down, and chromatin immunoprecipitation assays show that ligand activated VDR specifically interacts with HNF4[alpha] to block HNF4[alpha] interaction with coactivators. ERK1/2 inhibitors suppressed VDR interaction with HNF4[alpha]. Electrophoretic mobility shift assay and mutagenesis analyses have identified the negative VDR response elements that bind VDR/RXR[alpha] in the human CYP7A1 promoter. Chromatin immunoprecipitation assays show that LCA or 1[alpha], 25(OH)2-VD3 activated ERK1/2 stimulated the recruitment of VDR, RXR[alpha] and co-repressors, but decreased the occupancy of HNF4[alpha] and coactivators to human CYP7A1 chromatin, resulting in the inhibition of CYP7A1 gene transcription. In conclusion, LCA and 1[alpha], 25(OH)2-VD3 caused intracellular translocation of VDR to the plasma membrane and nucleus in human hepatocytes. LCA and VDR activated the c-Raf/MEK/ERK1/2 pathway to regulate VDR inhibition of CYP7A1 in hepatocytes. This study identified a novel bile acid-activated VDR signaling pathway in human hepatocytes. LCA activation of VDR may inhibit bile acid synthesis and protect hepatocytes from cholestatic liver injury.
Download or read book The Biology of Cholesterol and Related Steroids written by N. B. Myant and published by Butterworth-Heinemann. This book was released on 2014-04-24 with total page 925 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Biology of Cholesterol and Related Steroids focuses on the study of sterols in relation to living organisms. The publication first takes a look at the analysis of sterols and related steroids and the distribution of sterols and related steroids in nature, as well as the processes of extraction and separation and presence of sterols in plants, fungi, vertebrates, and invertebrates. The text then ponders on biosynthesis of sterols and metabolism of cholesterol. Topics include formation of fatty acid esters of cholesterol, steroid hormones, biosynthetic pathway to sterols, reaction mechanisms, and comparative aspects of sterol synthesis. The manuscript examines the developmental aspects of cholesterol metabolism and sterols in biological membranes. The book also reviews cholesterol synthesis in animal tissues, sterol metabolism in isolated cells, and epidemiology of the plasma cholesterol. Discussions focus on selection of statistical populations, genetic influences, regulation of sterol synthesis, general aspects of sterol metabolism, and removal of cell cholesterol in vivo. The publication is a dependable source of data for biochemists and readers interested in the biology of cholesterol and steroids.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 2000 with total page 1828 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The role of bile acid ba and related metabolites and hormone abnormalities in metabolic diseases written by Yanli Pang and published by Frontiers Media SA. This book was released on 2023-04-17 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 2164 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Download or read book A Comprehensive Guide to Toxicology in Preclinical Drug Development written by Ali S. Faqi and published by Academic Press. This book was released on 2012-11-02 with total page 904 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Comprehensive Guide to Toxicology in Preclinical Drug Development is designed for toxicologists who need a thorough understanding of the drug development process. This multi-contributed reference will provide a detailed picture of the complex and highly interrelated activities of preclinical toxicology in both small molecules and biologics --
Download or read book Bibliography of Agriculture written by and published by . This book was released on 1999 with total page 828 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Coronary Primary Prevention Trial written by and published by . This book was released on 1984 with total page 2 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Liver written by Irwin M. Arias and published by John Wiley & Sons. This book was released on 2020-03-09 with total page 1156 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bridging the gap between basic scientific advances and the understanding of liver disease — the extensively revised new edition of the premier text in the field. The latest edition of The Liver: Biology and Pathobiology remains a definitive volume in the field of hepatology, relating advances in biomedical sciences and engineering to understanding of liver structure, function, and disease pathology and treatment. Contributions from leading researchers examine the cell biology of the liver, the pathobiology of liver disease, the liver’s growth, regeneration, metabolic functions, and more. Now in its sixth edition, this classic text has been exhaustively revised to reflect new discoveries in biology and their influence on diagnosing, managing, and preventing liver disease. Seventy new chapters — including substantial original sections on liver cancer and groundbreaking advances that will have significant impact on hepatology — provide comprehensive, fully up-to-date coverage of both the current state and future direction of hepatology. Topics include liver RNA structure and function, gene editing, single-cell and single-molecule genomic analyses, the molecular biology of hepatitis, drug interactions and engineered drug design, and liver disease mechanisms and therapies. Edited by globally-recognized experts in the field, this authoritative volume: Relates molecular physiology to understanding disease pathology and treatment Links the science and pathology of the liver to practical clinical applications Features 16 new “Horizons” chapters that explore new and emerging science and technology Includes plentiful full-color illustrations and figures The Liver: Biology and Pathobiology, Sixth Edition is an indispensable resource for practicing and trainee hepatologists, gastroenterologists, hepatobiliary and liver transplant surgeons, and researchers and scientists in areas including hepatology, cell and molecular biology, virology, and drug metabolism.
Download or read book Cytochrome P450 Function and Pharmacological Roles in Inflammation and Cancer written by and published by Academic Press. This book was released on 2015-07-23 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytochrome P450 Function and Pharmacological Roles in Inflammation and Cancer, the latest volume in the Advances in Pharmacology series, presents not only the function of cytochrome P450 but also its pharmacological roles in inflammation and cancer. - Contains contributions from the best authors in the field - Ideal reference for those conducting research in cancer, inflammation, cytochrome P450, metabolism, liver disease, and oxidative stress - Provides an essential resource for pharmacologists, immunologists, and biochemists
Download or read book The Metabolism Structure and Function of Plant Lipids written by Paul K. Stumpf and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 653 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Seventh International Symposium on the Structure and Function of Plant Lipids took place at the University of California, Davis, California July 27th to August 1st, 1986. This was the first time the Symposium was held in the United States. The list of previous host cities reads, Norwich, Karlsruhe, Goteborg, Paris, Groningen, Neuchatel. The addition of Davis to this distinguished list was made by the organizers with the doubts of people who give invitations to parties - will anybody come? In fact 155 participants registered and there were 21 spouses in attendance. The scientific program was composed of nine sessions: biochemistry of isoprenoids and sterols, function of isoprenoids and sterols, structure and function of lipids, biosynthesis of complex lipids, fatty acid oxygenases and desaturases, medium and long chain fatty acids, interaction of university, government and industrial research, algal lipids, and genetics and biotechnology. In addition to these sessions of plenary lectures, there were four poster sessions in which about 140 posters were presented. All of this was packed into four days, and there was some comment about the scarcity of time to ask questions of the speakers, discuss the posters and even to eat lunch. The compression of the program was a result of the continued desire of the organizing committees to avoid concurrent sessions. The congregation of participants into a single session increases interaction and generates a feeling of unity at these symposia.
Download or read book A Comprehensive Guide to Toxicology in Nonclinical Drug Development written by Ali S. Faqi and published by Elsevier. This book was released on 2024-02-11 with total page 1074 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Comprehensive Guide to Toxicology in Nonclinical Drug Development, Third Edition is a valuable reference providing a complete understanding of all aspects of nonclinical toxicology in pharmaceutical research. This updated edition has been expanded and re-developed covering a wide-range of toxicological issues in small molecules and biologics. Topics include ADME in drug discovery, pharmacokinetics, toxicokinetics, formulations, and genetic toxicology testing. The book has been thoroughly updated throughout to reflect the latest scientific advances and includes new information on antiviral drugs, anti-diabetic drugs, immunotherapy, and a discussion on post-pandemic drug development challenges and opportunities. This is an essential and practical resource for all toxicologists involved in nonclinical testing in industry, academic, and regulatory settings. - Provides updated, unique content not covered in one comprehensive resource, including chapters on stem cells, antiviral drugs, anti-diabetic drugs, and immunotherapy - Includes the latest international guidelines for nonclinical toxicology in both small and large molecules - Incorporates practical examples in order to illustrate day-to-day activities and expectations associated with working in nonclinical toxicology
Download or read book Biomedical Index to PHS supported Research written by and published by . This book was released on 1993 with total page 1400 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Current Opinion in Lipidology written by and published by . This book was released on 1997 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Vitamin D in Clinical Medicine written by A. Giustina and published by Karger Medical and Scientific Publishers. This book was released on 2018-03-29 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vitamin D deficiency is prevalent today not only among the elderly but pervasively throughout all ages of life.This is due, in part, to systemic diseases that affect vitamin D metabolism, to changes in lifestyle, such as insufficient exposure to sunlight, and to increased use of sunscreen. Apart from the obvious effects of vitamin D deficiency on skeletal metabolism, the problem is assuming even greater significance because observational and interventional studies have linked vitamin D deficiency to cardiovascular disease, cancer, and diabetes. This book addresses a variety of important issues that have emerged from this fast-moving area of clinical medicine. The topics include assays of vitamin D and its binding protein, effects on aging and associated complications, primary and secondary states of altered parathyroid hormone secretion, vitamin D in the growing years of children and adolescents, nutritional requirements, and vitamin D as it relates to systemic disorders such as diabetes mellitus. Vitamin D in Clinical Medicine aims to offer new insights, in an evidence-based way, on important issues related to vitamin D. It is written for general practitioners and internists, as well as for endocrinologists, nutritionists, pulmonologists, cardiologists, and oncologists.
