Download or read book Imaging and Dynamic Studies of DNA During Capillary Electrophoresis written by Jeffrey James Schwinefus and published by . This book was released on 1999 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Capillary Electrophoresis of Nucleic Acids written by Keith R. Mitchelson and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.

Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses

Download or read book Investigation of DNA Electrophoretic Migration Dynamics by Capillary Electrophoresis and Three dimensional Fluorescence Video microscopy written by Olivia de Carmejane and published by . This book was released on 2001 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Analysis of Nucleic Acids by Capillary Electrophoresis written by Christoph Heller and published by Springer Science & Business Media. This book was released on 1997-06-26 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the last decade, capillary electrophoresis has been developed into a very powerful analytical technique, which has many advantages over conventional slab gel techniques. The improvement is comparable to the one occuring earlier in the field of chromatography, with the introduction of the high performance technology. How important this technique has be come, is reflected by the shear volume of papers published each month; as well as a dozen of books already published on this subject. One of the most important conferences in the field, the "International Symposium on High Performance Capillary Electrophoresis" attracts now nearly thousand people every year. As capillary electrophoresis can be applied to many different analytical problems, a spe cialization is unavoidable. This evolution is also reflected in the development of instrumen tation: whereas the first devices were designed for all possible applications, new instruments are now built, that are specialized for one particular task, e.g. DNA analysis. I very much welcome the decision of the series editor and the publisher, to edit a series ofspecialized books, covering all aspects of capillary electrophoresis. Having worked on the electrophoretic separation of DNA for many years, I am convinced that there are so many different aspects on this issue that they deserve a whole book on their own. Therefore, I was happy to agree when being asked to edit this book.

Download or read book Analysis of Nucleic Acids by Capillary Electrophoresis written by Christoph Heller and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Die Kapillarelektrophorese (CE) ist heute in biochemischen Laboratorien noch nicht als Routinemethode zur DNA-Trennung etabliert, obwohl sie diverse Vorzüge gegenüber den derzeitigen Methoden besitzt. In diesem Band stellen verschiedene Wissenschaftler die theoretischen Aspekte der Trennung von Oligonucleinsäuren mit der CE, die instrumentellen Grundlagen und deren Grenzen und schließlich die praktische Anwendung in der Biochemie und Molekularbiologie vor. This book on capillary electrophoresis is unique in its focus on the separation of nucleic acids. The importance of electrophoretic separation in every molecular biology laboratory justifies this specialization, which is also reflected in the development of instrumentation. This book is aimed to help to implement this rather new and promising technology in the biological laboratories and to help to overcome typical problems that can occur when starting with a new technique. It should also trigger further development in the field. It covers the theoretical background as well as practical examples of usual applications. The authors are all experts in their field, having many years of experience with capillary electrophoresis and their application to nucleic acids.

Download or read book Clinical Applications of Capillary Electrophoresis written by Stephen M. Palfrey and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 215 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Clinical Applications of Capillary Electrophoresis, Stephen Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. Written by the leading scientists who have often perfected these methods in their own laboratories, the protocols furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including urine protein analysis, hemoglobin separation, and the detection of CSF proteins, lipoproteins, myoglobin, cryoglobulins, HbA1c, and cathepsin. The protocols offered for DNA studies include double-stranded DNA analysis, the prenatal diagnosis of Down's syndrome, Rh D/d genotyping, the identification of mutated p53 oncogene, and the detection of microsatellite instability in cancers. Many of the methods can be automated to replace the more costly and labor-intensive tests that are currently used in most clinical laboratories. Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods. It offers to beginning clinical investigators, as well as established laboratories new to the technique, a representative range of highly practical CE methods-assays that are not only certain to become ever more productive, but are already eminently useful today.

Download or read book Capillary Electrophoresis of Nucleic Acids written by Keith R. Mitchelson and published by Humana Press. This book was released on 2010-10-28 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.

Download or read book Capillary Electrophoresis of DNA written by Richard W. Hammond and published by . This book was released on 1997 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Optimization of Capillary Electrophoresis with Imaging Detection for Estimation of Diffusion Coefficients written by Atefeh Sadat Zarabadi and published by . This book was released on 2015 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt: The diffusion coefficient, D, is an important physico-chemical property of the proteins. While it can be measured using various analytical techniques, capillary electrophoresis (CE) is widely used for this purpose. CE is particularly effective in combination with whole column imaging detection (WCID) technology has enabled the CE approach to measure the diffusion coefficient in shorter time. Thus far, however, WCID has rarely been used for monitoring diffusion processes. The purpose of this study was to optimize the application of capillary electrophoresis, coupled with imaging detection, to the problem of protein diffusion coefficient measurement. This optimization addressed both experimental and data analysis aspects of the method. For data analysis, we explored the use of Fourier transformation (FT), which offers a non-parametric, shape-independent evaluation of the raw data. FT was compared with two other common methods, both of which operate in the time domain and, unlike FT, operate in the time domain and make assumptions about the fundamental shape of the signal. As part of exploring FT data analysis, we performed simulations, in which parameters such as resolution and measurement time were systematically varied in order to find the optimal experimental conditions. Moreover, a new experimental approach is introduced for fast and simple measurement of diffusion coefficients, and which we refer to as imaging plug flow (iPF). This technique combines capillary electrophoresis, in which proteins are pre-concentrated under the membrane by electrokinetic injection, with a dynamic imaging detection. The concentration gradient detection method based on Schlieren optics is a universal, inexpensive, and efficient dynamic imaging technology for high-throughput moving boundary electrophoresis. A prototype multi-channel chip has been implemented for such measurements, and the effect of channel dimensions was investigated. The simultaneous measurement of the diffusion coefficients in the channels of this microfluidic device has improved the throughput of the experiment. The proposed approach addresses the limitation of single channel detection associated with UV whole column imaging detection. The implementation of a multi-channel CE chip, coupled with Schlieren imaging detection and an improved scheme for data analysis based on FT, provides a simple, fast, and high-throughput approach to the analysis of proteins diffusion coefficients.

Download or read book Capillary Electrophoresis of Nucleic Acids written by and published by Springer. This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Issues in Applied Analytical and Imaging Sciences Research 2013 Edition written by and published by ScholarlyEditions. This book was released on 2013-05-01 with total page 1173 pages. Available in PDF, EPUB and Kindle. Book excerpt: Issues in Applied, Analytical, and Imaging Sciences Research: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Applied Analysis. The editors have built Issues in Applied, Analytical, and Imaging Sciences Research: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Applied Analysis in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Applied, Analytical, and Imaging Sciences Research: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Download or read book Deoxyribonucleic Acid Mutation Detection Via Fluorescence Imaging in a Spatial Thermal Gradient Capillary Electrophoresis System written by Bryan Lee Crane and published by . This book was released on 2001 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Studying Protein DNA Interactions Using Kinetic Capillary Electrophoresis written by Alexander Petrov and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein-DNA interactions are essential to a cell as they control all vital cellular functions such as DNA replication, repair, recombination and transcription. In order to understand the dynamics of these fundamental biological processes, it is important to know kinetic parameters for all steps involved in the formation and dissociation of relevant DNA-protein complexes. We propose Kinetic Capillary Electrophoresis (KCE) as a conceptual platform for development of kinetic homogenous affinity methods for measuring kinetic parameters of such interactions. KCE is defined as the capillary electrophoresis (CE) separation of species that interact during electrophoresis. All KCE methods are described by the same mathematics: the same system of partial differential equations with only initial and boundary conditions being different. One of the advantages of KCE is that mathematical modeling is not necessary for some of them. Here, I i) present the theoretical bases of KCE and propose a multi-method KCE toolbox as an integrated kinetic technique, ii) develop a new KCE method with simplified mathematical approach, iii) introduce a predictive mixing parameter that is essential for diffusion-based mixing in general and to KCE in particular, iv) demonstrate the advantage of the proposed concept for studying complex protein-DNA interactions. Being based on CE separation, KCE is a powerful tool for testing different hypotheses of interactions and accurately calculating multiple binding parameters such as i) high-affinity (specific) and low-affinity (non-specific) interactions that recur within the same protein-DNA pair ii) disassembly of DNA-multiple proteins complexes. The concept of KCE allows for the creation of an expanding toolset of powerful kinetic homogeneous affinity methods, which will find their applications in studies of biomolecular interactions, quantitative analyses, and selecting affinity probes and drug candidates from complex mixtures.

Download or read book Lasers and Current Optical Techniques in Biology written by Giuseppe Palumbo and published by Royal Society of Chemistry. This book was released on 2007-10-31 with total page 684 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of innovative light sources, fibre laser sources and light emitting diodes, is opening unexpected perspectives into optical techniques and is promising new exciting applications in the field of biomedicine. Lasers and Current Optical Techniques in Biology aims to provide an overview of light sources, together with an extensive and authoritative description of the optical techniques in bio-medicine. This book is designed to give biomedical researchers a strong feel for the capability of physical approaches, promote new interdisciplinary interests and persuade more practitioners to take advantage of optical techniques. Current developments in a variety of optical techniques, including Near-Infra Red Spectroscopy, and traditional and advanced fluorescence techniques are covered, ranging from those that are becoming common practice to those that need much more experimentation before they can be accepted as real breakthroughs. Further topics include optical coherence tomography and its variations, polarised light imaging and, principle laser and lamp sources- a usually fragmentary topic, often dispersed among specialist publications. The wide range of topics covered make Lasers and Current Optical Techniques in Biology of interest to a diverse range of scientific communities.

Download or read book Biomedical Index to PHS supported Research pt A Subject access A H written by and published by . This book was released on 1992 with total page 1064 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Cumulated Index Medicus written by and published by . This book was released on 2000 with total page 1840 pages. Available in PDF, EPUB and Kindle. Book excerpt: