Download or read book Identification and Characterizaton of Genes Required for Cell Viability in the Absence of the Swi6 Protein in Saccharomyces Cerevisiae written by Neil N. Macpherson and published by . This book was released on 1999 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 'Saccharomyces cerevisiae', the Swi6 protein is a component of two transcription factors, SBF and MBF, that promote gene expression in late G1 phase of the cell cycle. In SBF, Swi6 interacts with the DNA-binding protein Swi4 to promote activation of the G1 cyclin-encoding genes ' CLN1, CLN2, PCL1' and 'PCL2', the 'HO' endonuclease-coding gene and cell wall biosynthesis genes. In MIBF, Swi6 is associated with a related DNA-binding protein, Mbp1, and activates transcription of DNA synthesis genes. Although SBF and MBF are required for cell viability, ' SWI6' is not an essential gene. I performed a synthetic lethal screen to identify genes that are required for viability in the absence of 'SWI6'. I identified ten complementation groups of 's_wi6'-dependent l_ethal m_utants, designated 'SLM1' through 'SLM10'. Members of the 'SLM2' complementation group may contain mutations in ' SWI2', since a 'slm2 swi6' mutant is rescued by overexpression of 'SWI2'. SWI2 encodes a component of the Swi/Snf chromatin-remodefing complex. The 'slm10' mutants contain mutations that are allelic to the 'PHO85' gene. I was most interested in mutants showing a cell cycle arrest phenotype; ' swi6' strains carrying mutations in 'slm7' and ' slm8' arrested in G1 phase under restrictive conditions. Analysis of the transcript levels of cell cycle-regulated genes in 'slm7' mutant strains revealed defects in regulation of a subset of cyclin genes. Complementation and allelism tests showed that 'SLM7' is allelic to the 'TAF17' gene, which encodes a protein that is a component of the basal transcription factor TFIID and a histone acetyl transferase complex. Sequencing showed that the 'slm7-1' allele of 'TAF17 ' is predicted to encode a version of Taf17 that is truncated within a highly conserved region. The cell cycle and transcriptional defects caused by the 'slm7-1' allele of 'TAF17' are consistent with the role of TAFs as modulators of transcriptional activation, and may reflect a role for 'TAF17' in modulating transcriptional activation by SBF and MBF. My discovery of a genetic interaction between 'TAF17 ' and 'SWI6' may reflect a novel role for basal transcription factors in G1-specific transcription, and may implicate chromatin remodeling in cell cycle-dependent transcription.

Download or read book Identification and Characterizaton of Genes Required for Cell Viability in the Absence of the Swi6 Protein in Saccharomyces Cerevisiae written by and published by . This book was released on 1999 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Characterizaton of Genes Required for Cell Viability in the Absence of the Swi6 Protein in Saccharomyces Cerevisiae microform written by Neil N. (Neil Norman) Macpherson and published by National Library of Canada = Bibliothèque nationale du Canada. This book was released on 1999 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Identification and Characterization of New Genetic Requirements for Proper Cell Type Identity in Saccharomyces Cerevisiae written by Sonia Santa Anna-A. and published by . This book was released on 1991 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Characterization of Genes Required for Early Meiotic Gene Expression in the Yeast Saccharomyces Cerevisiae written by Sophia Seh-Yi Su and published by . This book was released on 1993 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book American Doctoral Dissertations written by and published by . This book was released on 1999 with total page 848 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Dissertation Abstracts International written by and published by . This book was released on 2003 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Characterization of Genes Important for Polarized Morphogenesis in Saccharomyces Cerevisiae written by Scott Bidlingmaier and published by . This book was released on 2003 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetics Abstracts written by and published by . This book was released on 2000 with total page 764 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Cumulated Index Medicus written by and published by . This book was released on 1989 with total page 1400 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Regulation of G1 Phase Progression written by Johannes Boonstra and published by Springer Science & Business Media. This book was released on 2003-09-30 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this contribution, several specialists describe the current knowledge on the molecular networks that regulate cell cycle progression, with an emphasis on the G1 phase of the cell cycle. The first part of Regulation of G1 Phase Progression is concerned with the individual molecules that form the network, including cyclins, cyclin-dependent kinases, inhibitors of these kinases and retinoblastoma and p53. The second section describes the signaling cascades by which external factors influence the cell cycle network, including mitogens, the extracellular matrix, nutrients and oxygen radicals. The last section describes the effects of specific external conditions on cell cycle progression and are presented such as serum starvation and subsequent re-addition and stress conditions (heat, osmolarity). The final two chapters describe the relation between cell cycle progression with cell differentiation and with apoptosis.

Download or read book Guide to Yeast Genetics and Molecular Biology written by Christine Guthrie and published by . This book was released on 1991-01-28 with total page 933 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Basic Methods in Yeast Genetics**Physical and genetic mapping**Making and recovering mutants**Cloning and Recombinant DNA Methods**High-efficiency transformation**Preparation of yeast artificial chromosome vectors**Basic Methods of Cell Biology**Immunomicroscopy**Protein targeting assays**Biochemistry of Gene Expression**Vectors for regulated expression**Isolation of labeled and unlabeled DNA, RNA, and protein

Download or read book Proceedings of the National Academy of Sciences of the United States of America written by National Academy of Sciences (U.S.) and published by . This book was released on 1992 with total page 1308 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Proceedings of the National Academy of Sciences (PNAS) publishes research reports, commentaries, reviews, colloquium papers, and actions of the Academy. PNAS is a multidisciplinary journal that covers the biological, physical, and social sciences.

Download or read book Agrindex written by and published by . This book was released on 1993 with total page 862 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book RNA Exosome written by Torben Heick Jensen and published by Springer Science & Business Media. This book was released on 2011-06-29 with total page 161 pages. Available in PDF, EPUB and Kindle. Book excerpt: The diversity of RNAs inside living cells is amazing. We have known of the more “classic” RNA species: mRNA, tRNA, rRNA, snRNA and snoRNA for some time now, but in a steady stream new types of molecules are being described as it is becoming clear that most of the genomic information of cells ends up in RNA. To deal with the enormous load of resulting RNA processing and degradation reactions, cells need adequate and efficient molecular machines. The RNA exosome is arising as a major facilitator to this effect. Structural and functional data gathered over the last decade have illustrated the biochemical importance of this multimeric complex and its many co-factors, revealing its enormous regulatory power. By gathering some of the most prominent researchers in the exosome field, it is the aim of this volume to introduce this fascinating protein complex as well as to give a timely and rich account of its many functions. The exosome was discovered more than a decade ago by Phil Mitchell and David Tollervey by its ability to trim the 3’end of yeast, S. cerevisiae, 5. 8S rRNA. In a historic account they laid out the events surrounding this identification and the subsequent birth of the research field. In the chapter by Kurt Januszyk and Christopher Lima the structural organization of eukaryotic exosomes and their evolutionary counterparts in bacteria and archaea are discussed in large part through presentation of structures.

Download or read book Index Medicus written by and published by . This book was released on 2002 with total page 1568 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.

Download or read book Metabolism and Molecular Physiology of Saccharomyces Cerevisiae written by J. Richard Dickinson and published by CRC Press. This book was released on 2004-04-27 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the best-selling first edition, much has been discovered about Saccharomyces cerevisiae, the single-celled fungus commonly known as baker's yeast or brewer's yeast that is the basis for much of our understanding of the molecular and cellular biology of eukaryotes. This wealth of new research data demands our attention and r