Download or read book Identification and Characterization of the Red Clover Necrotic Mosaic Virus Origin of Assembly written by Veronica Roshani Basnayake and published by . This book was released on 2005 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt: Keywords: icosahedral, RNA, plant virus, packaging scheme, origin of assembly, Red clover necrotic mosaic virus.

Download or read book Identification and Characterization of the Red Clover Necrotic Mosaic Virus Origin of Assembly written by and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Red clover necrotic mosaic virus (RCNMV) is a single-stranded positive-sense RNA plant virus of the Dianthovirus genus, family Tombusviridae. Each RCNMV virion contains 180 subunits of the 37 kDa capsid protein (CP) forming a non-enveloped, isometric particle of T=3 quasi symmetry, 30-35 nm in diameter. The RCNMV genome consists of two RNAs, RNA-1 and RNA-2. RNA-1 codes for three proteins: i) p88, the RNA-dependent RNA polymerase, ii) p27, the replicase related protein and iii) the CP. RNA-2 is monocistronic and codes for the movement protein (MP). Currently, the RNA complement within RCNMV virions has not been determined. Density gradient centrifugation data has suggested the probability of a single type of particle, whereas the viral RNA profile from virions suggests otherwise. My thesis research has determined the RNA content within RCNMV virions after exposure to either heat or UV-irradiation. Both treatments result in the formation of a stable RNA-1: RNA-2 heterodimer. This leads to the conclusion that RCNMV virions co-package RNA-1 and RNA-2. Upon either treatment, RNA-2 multimers are also observed, suggesting the presence of particles packaging RNA-2 exclusively. These observations suggest that the RCNMV virion population consists of two distinct types of particles having similar densities. Based on the above RNA complement findings, I proceeded to delineate the specific viral sequences that determine the RNA content of an RCNMV virion. During virion assembly, the RCNMV CP must be able to distinguish and package both RNA-1 and RNA-2 while excluding heterologous host RNAs present in the cell. Viral CP subunits recognize specific cognate genomic sequences and/or structures that are unique to each viral genome. These are designated as origin of assembly sequences or packaging signals. To elucidate the assembly mechanism of RCNMV, I searched for the presence of distinct packaging signals on each of the genomic RNAs. Various constructs of RNA-1 and RNA-2 were test.

Download or read book Structural Characterization of Red Clover Necrotic Mosaic Virus by Small Angle Neutron Scattering and X ray Crystallography written by Stanton Martin Lowell and published by . This book was released on 2012 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Red Clover Necrotic Mosaic Dianthovirus Origin of Assembly Sequence written by Sandra J. W. Mackie and published by . This book was released on 1999 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biological Agricultural Index written by and published by . This book was released on 1995 with total page 3036 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Cumulated Index Medicus written by and published by . This book was released on 1969 with total page 1264 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Plant Viruses written by B.D. Harrison and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fifth volume in the series The Plant Viruses, dealing with viruses with bipartite genomes, completes the coverage of viruses with isometric parti cles and genomes consisting of single-stranded, positive-sense RNA: viruses that have tripartite and monopartite genomes of this kind were dealt with in Volumes 1 and 3, respectively. How close are the affinities among the viruses within the groupings distinguished in this way? All those with tripartite genomes are considered to be sufficiently closely related to be included in the family Bromoviridae, whereas the monopartite-genome viruses covered in Volume 3 clearly are a much more diverse collection. Affinities among the viruses with bipartite genomes are considered in Chapter 1 of this volume, along with the possible origins, advantages, and disadvantages of these ge nomes. The conclusion reached from this assessment is that the bipartite genome viruses fall into four categories, those within each category having closer affinities with viruses not included in this book than with viruses in the other categories. No evidence was found that possession of a bipartite genome gives a virus overwhelming advantages over viruses of other sorts. More probably, any advantages are largely balanced by disadvantages, and bipartite genomes may be best considered simply as an alternative design for the hereditary material of a virus.

Download or read book Virology AIDS Abstracts written by and published by . This book was released on 1994 with total page 972 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Cambridge Scientific Biochemistry Abstracts written by and published by . This book was released on 1993-10 with total page 1046 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Fields Virology written by David Mahan Knipe and published by Lippincott Williams & Wilkins. This book was released on 2007 with total page 3116 pages. Available in PDF, EPUB and Kindle. Book excerpt: Accompanying CD-ROM has same title as book.

Download or read book American Society for Virology 22nd Annual Meeting written by American Society for Virology. Meeting and published by . This book was released on 2003 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Virology Abstracts written by and published by . This book was released on 1987 with total page 1018 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Agrindex written by and published by . This book was released on 1995 with total page 1064 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 2292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.

Download or read book Bibliography of Agriculture written by and published by . This book was released on 1991 with total page 1810 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Plant Immunity against Viruses written by Yule Liu and published by Frontiers Media SA. This book was released on 2017-09-28 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant viruses impose a serious threat on agriculture, which motivates extensive breeding efforts for viral resistant crops and inspires lasting interests on basic research to understand the mechanisms underlying plant immunity against viruses. Viruses are obligate intracellular parasites. Their genomes are usually small and only encode a few products that are essential to hijack host machinery for their nucleotide and protein biosynthesis, and that are necessary to suppress host immunity. Plants evolved multilayers of defense mechanisms to defeat viral infection. In this research topic, we gathered 13 papers covering recent advances in different aspects of plant immunity against viruses, including reviews on RNA silencing and R gene based immunity and their application, translational initiation factor mediated recessive resistance, genome editing based viral immunity, role of chloroplast in plant-virus interaction, and research articles providing new mechanistic insights on plant-virus interactions. We hope that this Research Topic helps readers to have a better understanding of the progresses that have been made recently in plant immunity against viruses. A deeper understanding of plant antiviral immunity will facilitate the development of innovative approaches for crop protections and improvements.

Download or read book Dissertation Abstracts International written by and published by . This book was released on 2003 with total page 664 pages. Available in PDF, EPUB and Kindle. Book excerpt: