Download or read book Identification and Characterization of Esophageal Squamous Cell Carcinoma Cancer Stem Cells and Their Chemoradiation Therapy written by 郭佩芳 and published by . This book was released on 2013 with total page 94 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification and Characterization of Tumor Suppressor Gene and Cancer Stemness Gene in Esophageal Squamous Cell Carcinoma written by Liyi Zhang and published by Open Dissertation Press. This book was released on 2017-01-27 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Identification and Characterization of Tumor Suppressor Gene and Cancer Stemness Gene in Esophageal Squamous Cell Carcinoma" by Liyi, Zhang, 張麗儀, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Esophageal squamous cell carcinoma (ESCC), the major histological subtype of esophageal cancer, is one of the most common malignancies with poor prognosis in the world. Despite continued development of diagnosis and treatment, ESCC remains the sixth leading cause of cancer death worldwide. Current treatment regimens in ESCC are often characterized by ineffectiveness and poor selectivity. Therapeutic methods directed at cancer-associated genes or cancer stem cells (CSCs) may be effective approaches to cure this deadly cancer. Therefore, this study aims to identify specific ESCC-related genes and cancer stemness genes which help us to develop new targeted agents to achieving objective, long-lasting therapeutic responses in ESCC. To obtain an accurate overview of genetic changes occurring in ESCC patients, our group performed microarray-based mRNA expression profiling and high-throughout transcriptome sequencing (RNA-Seq) to compare differentially expressed genes between ESCC tumors and their corresponding non-tumorous tissues. Prostate stem cell antigen (PSCA) was considered to be a candidate of primary interest due to significantly reduced expression in both microarray and RNA-Seq data. In this study, we examined the role of PSCA on the pathogenesis of esophageal cancer. Our results showed that PSCA was frequently down-regulated in ESCC. Its expression was negatively regulated by transcription factor SOX5. Also, we provided evidence that down-regulation of PSCA was associated with poor clinical outcomes of patients with ESCC. Both in vitro and in vivo assays revealed that PSCA could arrest cell cycle progression and promote differentiation. To further elucidate the mechanism involved in biological function of PSCA, we performed co-immunoprecipitation and mass spectroscopy to identify proteins that associate with PSCA. This study found that RB1CC1, a key signaling node to regulate cellular proliferation and differentiation, interacted specifically with PSCA both in vitro and in vivo. Binding of PSCA and RB1CC1 in cytoplasm resulted in stabilization and translocation of RB1CC1 into nucleus and then further regulates the crucial cell cycle and differentiation genes. Furthermore, in order to identify the cancer stemness genes specifically expressed in CSCs of ESCC, we utilized gene expression analysis to profile 34 stemness-associated genes in ESCC specimens. Developmental pluripotency associated 4 (DPPA4), a well known pluripotent marker of stem cell, was considered as the best candidate. Our following histopathological study demonstrated that DPPA4 rigorously marked the rare CSCs, in contrast to core stemness factors (OCT4 and SOX2) and previous reported CSC markers (CD90 and CD44), which expressed in a large population of cancer cells. Moreover, the expression of DPPA4 was also found to have prognostic value in ESCC, as the appearance of DPPA4+ cells was significantly associated with poor differentiation, advanced stage and higher incidences of lymph node metastasis. Finally, our functional studies showed that ESCC cells expressing exogenous DPPA4 conferred an enhanced ability to initiate tumor, self-renew, resist chemotherapy and metastasize through lymphatic system. In summary, this study provide evidence indicating that novel tumor suppressor gene PSCA and cancer stemness gene DPPA4 may contribute to the development and pr
Download or read book Identification and Characterization of Cancer Related Genes in Esophageal Squamous Cell Carcinoma written by Li Fu and published by Open Dissertation Press. This book was released on 2017-01-27 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Identification and Characterization of Cancer-related Genes in Esophageal Squamous Cell Carcinoma" by Li, Fu, 付利, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled Identification and characterization of cancer-related genes in esophageal squamous cell carcinoma Submitted by Fu Li For the degree of Doctor of Philosophy at The University of Hong Kong in June 2007 Esophageal squamous cell carcinoma (ESCC) is the sixth leading cause of cancer death worldwide and the fourth most common malignancies in China. Previous studies have shown that ESCC is a lethal disease caused by a combination of environmental and genetic risk factors. However, the genetic steps involved in carcinogenesis of ESCC are still unclear. This study aims to identify more ESCC-related genes and to characterize their roles in the tumorigenesis of ESCC. Deletion of chromosome 3p is common in ESCC, suggesting the existence of one or more tumor suppressor genes (TSGs) at this region. Single-nucleotide polymorphism (SNP)-mass spectrometry-genotyping (SMSG), was applied to investigate loss of heterozygosity (LOH) on 3p in 100 primary ESCC cases with 386 SNP markers. The accuracy of LOH frequency detected by SMSG was further validated by quantitative real-time PCR in five selected SNP loci and the results demonstrated that SMSG is a reliable tool for LOH screening. Four minimal deleted regions (MDRs) at 3p26.3, 3p22, i3p21.3 and 3p14.2 were identified. Absent and down-regulated expression of candidate TSGs from MDRs and other frequent LOH loci, including CHL1, APG7L, PCAF, RBMS3, PLCD1 and CACNA2D3, were detected in primary ESCC tumors and ESCC cell lines. Deletions of MDR-1, 2 and 4 correlated with both advanced tumor stage and poor differentiation in ESCC further strengthened the clinical relevance to these deleted regions. A potential TSG located at 3p22, Phospholipase C-δ1 (PLCD1), was subsequently characterized. Absent expression of PLCD1 was detected in 26/50 (52%) primary ESCCs and 4/9 (44.4%) ESCC cell lines, which was significantly associated with DNA- copy-number loss and promoter hypermethylation (P The majority of ESCC patients presented with advanced/metastatic disease upon diagnosis let us to identify new biomarkers for molecular tumor staging and prognostic evaluation. Differentially expressed proteins among different stages of primary ESCCs and their paired nontumorous tissues were compared by proteomics-based technology. Among 18 differentially expressed proteins, the expression levels of alpha-actinin 4 ii(ACTN4) and 67 kDa laminin receptor (67LR) progressively increased from stage I to III. Clinicopathological correlation study using TMA revealed that ACTN4 overexpression was significantly associated with advanced clinical stage and lymph node metastasis while 67LR overexpression was significantly correlated with advanced clinical stage. In summary, this study provided evidence that more ESCC-associated 3p
Download or read book Identification and Characterization of Cancer related Genes in Esophageal Squamous Cell Carcinoma written by Li Fu (Ph. D.) and published by . This book was released on 2007 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Esophageal Squamous Cell Carcinoma written by Nobutoshi Ando and published by Springer. This book was released on 2014-10-22 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: Esophageal cancer causes an estimated 386,000 deaths worldwide and is the sixth most common cause of death for men. The background characteristics of esophageal cancer treatment are markedly different between Asian and Western countries, however. In tumor histology, squamous cell carcinoma associated with smoking and alcohol consumption is overwhelmingly prevalent in Asia, whereas adenocarcinoma associated with Barrett’s metaplasia is markedly prevalent in the West. In Asia, especially in Japan, the key persons who play important roles in the management of esophageal cancer patients are surgeons; in the West those roles are filled by medical and radiation oncologists as well as surgeons. The philosophy of surgeons regarding cancer surgery varies from locoregional to local tumor control, particularly in focusing on lymph node dissection. Physicians’ approach to surgical adjuvant therapy differs, therefore, between Asia and the West. Considering these East–West differences in esophageal cancer treatment, the currently available results of Western evidence should not be considered directly applicable to esophageal cancer in Asia. In this book, the authors discuss the knowledge base in Japan in terms of treatment of esophageal squamous cell carcinoma. Since this volume contains a wide spectrum of current information and addresses topics surrounding the treatment of patients with esophageal squamous cell carcinoma, it is highly relevant to Asian physicians and researchers as well as to their counterparts in the West.
Download or read book Identification and Characterization of Tumor Suppressor Gene and Cancer Stemness Gene in Esophageal Squamous Cell Carcinoma written by 張麗儀 and published by . This book was released on 2015 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification and Characterization of Tumor Suppressive Gene and Microrna in Esophageal Squamous Cell Carcinoma written by Kar-Lok Kong and published by Open Dissertation Press. This book was released on 2017-01-26 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Identification and Characterization of Tumor Suppressive Gene and MicroRNA in Esophageal Squamous Cell Carcinoma" by Kar-lok, Kong, 江家樂, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. DOI: 10.5353/th_b4645569 Subjects: Small interfering RNA Antioncogenes Esophagus - Cancer - Genetic aspects
Download or read book Identification and Characterization of Chl1 in Esophageal Squamous Cell Carcinoma written by Cailei Zhu and published by Open Dissertation Press. This book was released on 2017-01-26 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Identification and Characterization of CHL1 in Esophageal Squamous Cell Carcinoma" by Cailei, Zhu, 祝彩磊, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. DOI: 10.5353/th_b4632955 Subjects: Antioncogenes Esophagus - Cancer - Genetic aspects
Download or read book Identification and Characterization of Tumor Suppressive Gene and MicroRNA in Esophageal Squamous Cell Carcinoma written by Kar-lok Kong and published by . This book was released on 2011 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Detection of Circulating Cancer Cells with Cancer Stem Cell Or Mesenchymal Characteristics in Esophageal Squamous Cell Carcinoma ESCC written by 谭圳 and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification and Characterization of Cancer Stem Cells Using Flow Cytometry written by Yasunari Kanda and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Identification and Characterization of Cancer Stem Cells Using Flow Cytometry.
Download or read book Identification and Characterization of CHL1 in Esophageal Squamous Cell Carcinoma written by Cailei Zhu and published by . This book was released on 2010 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cancer Stem Cells written by Sharmila A. Bapat and published by Wiley-Interscience. This book was released on 2008-10-20 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because the concept and discoveries of cancer stem cells are relatively new, scientists and researchers need an introduction to this dynamic area. Cancer Stem Cells presents a consolidated account of the research done to date and recent progresses in the studies of cancer stem cells. Such a presentation facilitates a better understanding of and draws attention to stem cell and cancer biology - two fields that enhance, move, and evolve into each other continuously. It provides an informative study in designing approaches to apply stem cell principles to cancer biology while offering an overview of the challenges in developing combination stem and cancer biology targets for therapeutics. This book serves as a primer for new researchers in the field of cancer biology.
Download or read book Identification and Characterization of Cancer Stem Cells in Human Anaplastic Thyroid Carcinoma written by Susan Friedman and published by . This book was released on 2008 with total page 46 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Esophageal Squamous Cell Carcinoma written by and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the second edition of the book, covering a wide spectrum of the latest information relevant to diagnosis and treatment of esophageal squamous cell carcinoma (ESCC). In recent years the incidence rate of esophageal cancer has been increasing; however, the background characteristics of the cancer treatment are significantly different between Asian and Western countries. In tumor histology, ESCC associated with smoking and alcohol consumption is overwhelmingly prevalent in Asia, whereas adenocarcinoma associated with Barretts metaplasia is remarkably prevalent in the West. In Asia, especially in Japan, surgeons play a significant role in the management of esophageal cancer patients, while medical and radiation oncologists as well as surgeons are important in the West. Considering these East-West differences in management of esophageal cancer, evidence originating in Asia should be more widely disseminated globally. The contributing authors, who have great expertise in their areas of specialization, discuss details in terms of treatment of ESCC, including basic science, diagnosis, surgery, other treatment modalities, Japanese guidelines, and also valuable experiences from other Asian countries. Accordingly, this excellent collection of texts benefits not only oncologists, but all medical and biological researchers involved in the latest ESCC research.
Download or read book Tumor Organoids written by Shay Soker and published by Humana Press. This book was released on 2017-10-20 with total page 225 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cancer cell biology research in general, and anti-cancer drug development specifically, still relies on standard cell culture techniques that place the cells in an unnatural environment. As a consequence, growing tumor cells in plastic dishes places a selective pressure that substantially alters their original molecular and phenotypic properties.The emerging field of regenerative medicine has developed bioengineered tissue platforms that can better mimic the structure and cellular heterogeneity of in vivo tissue, and are suitable for tumor bioengineering research. Microengineering technologies have resulted in advanced methods for creating and culturing 3-D human tissue. By encapsulating the respective cell type or combining several cell types to form tissues, these model organs can be viable for longer periods of time and are cultured to develop functional properties similar to native tissues. This approach recapitulates the dynamic role of cell–cell, cell–ECM, and mechanical interactions inside the tumor. Further incorporation of cells representative of the tumor stroma, such as endothelial cells (EC) and tumor fibroblasts, can mimic the in vivo tumor microenvironment. Collectively, bioengineered tumors create an important resource for the in vitro study of tumor growth in 3D including tumor biomechanics and the effects of anti-cancer drugs on 3D tumor tissue. These technologies have the potential to overcome current limitations to genetic and histological tumor classification and development of personalized therapies.
Download or read book Atlas of Minimally Invasive Surgery for Lung and Esophageal Cancer written by Jun Wang and published by Springer. This book was released on 2017-05-15 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Atlas presents a state-of-the-art review of VATS and robotic approaches to managing lung and esophageal cancers. It discusses cancer staging, physiological evaluation of patients, and patient selection for minimally invasive surgery. The atlas offers detailed descriptions of individual operations accompanied by anatomic drawings, intraoperative images, and 3-dimensional anatomic reconstructions. Written by recognized experts in the field, it provides readers with an unparalleled resource for advancing their skills in managing these cancers. It is a valuable reference work for thoracic surgeons in training as well as in practice who want to pursue minimally invasive surgery. It is unique in offering fully illustrated, step-by-step descriptions of the operative procedures.
