Download or read book Ice Cold Replica 10 written by Marilyn Kaye and published by Skylark. This book was released on 2013-09-25 with total page 107 pages. Available in PDF, EPUB and Kindle. Book excerpt: Amy's secret is out! Her worst enemy, Jeanine, knows that Amy is a clone. Now Amy fears the worst. She's sure Jeanine will blab the truth to everyone--maybe even try to sell Amy's story to some sleazy tabloid. But Jeanine never gets a chance to make good on any of her threats. An accident leaves her in a coma. Rumors of foul play spread like wildfire. The number one suspect . . . Amy. Sure, Amy wanted to stop Jeanine's big mouth, but maybe she wasn't the only one. . . .
Download or read book Collected Reprints written by Atmospheric Physics and Chemistry Laboratory (U.S.) and published by . This book was released on 1973 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Collected Reprints Atmospheric Physics and Chemistry Laboratory written by Atmospheric Physics and Chemistry Laboratory (U.S.) and published by . This book was released on 1974 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cloning written by Stephen D. Fairbanks and published by Nova Publishers. This book was released on 2004 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: The terms 'recombinant DNA technology', 'DNA cloning', 'molecular cloning' or 'gene cloning' all refer to the same process: the transfer of a DNA fragment of interest from one organism to a self-replicating genetic element such as a bacterial plasmid. The DNA of interest can then be propagated in a foreign host cell. This technology has been around since the 1970s, and it has become a common practice in molecular biology labs today. Reproductive cloning is a technology used to generate an animal that has the same nuclear DNA as another currently or previously existing animal. Dolly was created by reproductive cloning technology. In a process called 'somatic cell nuclear transfer' (SCNT), scientists transfer genetic material from the nucleus of a donor adult cell to an egg whose nucleus, and thus its genetic material, has been removed. The reconstructed egg containing the DNA from a donor cell must be treated with chemicals or electric current in order to stimulate cell division. Once the cloned embryo reaches a suitable stage, it is transferred to the uterus of a female host where it continues to develop until birth. Therapeutic cloning, also called "embryo cloning," is the production of human embryos for use in research. The goal of this process is not to create cloned human beings, but rather to harvest stem cells that can be used to study human development and to treat disease. Stem cells are important to biomedical researchers because they can be used to generate virtually any type of specialised cell in the human body. This new book presents an up-to-date Chronology of Cloning along with current and selected abstracts dealing with cloning as well as a guide to books on the topic. Access to the abstract and books sections is provided by title, subject and author indexes.
Download or read book Atmospheric and Physics and Chemistry Laboratory written by Environmental Research Laboratories (U.S.) and published by . This book was released on 1974 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Antibody Engineering Protocols written by Sudhir Paul and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive collection of recently developed methods for producing new antibody reagents by immunization and recombinant DNA techniques contains ready-to-use protocols that illuminate current areas of research on antibody structure, functions, and applications. The methods can be applied in basic immunological studies involving antibody specificity, catalysis, and evolution, and in the isolation of rare antibodies by phage display technology and the engineering of new antibodies by mutagenesis. They offer insight into new ways of developing clinically useful antibody reagents. Antibody Engineering Protocols constitutes a single-source volume for laboratory investigators who want to minimize extensive literature and methodology searches and to work productively in their fields with reproducible step-by-step protocols.
Download or read book Human Papillomaviruses written by Clare Davy and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular MedicineTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Download or read book Colloidal Gold written by and published by Elsevier. This book was released on 2012-12-02 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This third volume completes Hayat's coverage of the principles and methodology of colloidal gold in microscopy. The three-volume set should become the standard reference in the field. Among the first books to cover principles and methodology of colloidal gold in microscopy Describes methods step by step to enable researchers to learn these complex procedures solely by reference to the book Discusses problems and limitations of techniques Guides users to avoid problems and choose the correct procedures for specific applications Contributors are eminent authorities in their fields Topics covered in Volume 3 include: Production of monoclonal and polyclonal antibodies, Lowicryl, polar, and apolar resins Preserving and localizing antigenicity; sectioning; high-resolution morphological localization Diaminobenzidine-colloidal gold; microinjection of colloidal gold; double labeling with backscattered electrons; microwave irradiation Applications to biomedical studies Colloidal gold in conjunction with video-enhanced microscopy
Download or read book The Assemblers 1 written by Andy Dudak and published by Caliber Comics. This book was released on 2020-03-18 with total page 29 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Immune system modeling and analysis written by Ramit Mehr and published by Frontiers Media SA. This book was released on 2015-04-22 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: The rapid development of new methods for immunological data collection – from multicolor flow cytometry, through single-cell imaging, to deep sequencing – presents us now, for the first time, with the ability to analyze and compare large amounts of immunological data in health, aging and disease. The exponential growth of these datasets, however, challenges the theoretical immunology community to develop methods for data organization and analysis. Furthermore, the need to test hypotheses regarding immune function, and generate predictions regarding the outcomes of medical interventions, necessitates the development of mathematical and computational models covering processes on multiple scales, from the genetic and molecular to the cellular and system scales. The last few decades have seen the development of methods for presentation and analysis of clonal repertoires (those of T and B lymphocytes) and phenotypic (surface-marker based) repertoires of all lymphocyte types, and for modeling the intricate network of molecular and cellular interactions within the immune systems. This e-Book, which has first appeared as a ‘Frontiers in Immunology’ research topic, provides a comprehensive, online, open access snapshot of the current state of the art on immune system modeling and analysis.
Download or read book Gene Targeting written by Alexandra Joyner and published by OUP Oxford. This book was released on 1999-12-09 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the first edition of Gene Targeting: A Practical Approach in 1993 there have been many advances in gene targeting and this new edition has been thoroughly updated and rewritten to include all the major new techniques. It provides not only tried-and-tested practical protocols but detailed guidance on their use and applications. As with the previous edition Gene Targeting: A Practical Approach 2e concentrates on gene targeting in mouse ES cells, but the techniques described can be easily adapted to applications in tissue culture including those for human cells. The first chapter covers the design of gene targeting vectors for mammalian cells and describes how to distinguish random integrations from homologous recombination. It is followed by a chapter on extending conventional gene targeting manipulations by using site-specific recombination using the Cre-loxP and Flp-FRT systems to produce 'clean' germline mutations and conditionally (in)activating genes. Chapter 3 describes methods for introducing DNA into ES cells for homologous recombination, selection and screening procedures for identifying and recovering targeted cell clones, and a simple method for establishing new ES cell lines. Chapter 4 discusses the pros and cons or aggregation versus blastocyst injection to create chimeras, focusing on the technical aspects of generating aggregation chimeras and then describes some of the uses of chimeras. The next topic covered is gene trap strategies; the structure, components, design, and modification of GT vectors, the various types of GT screens, and the molecular analysis of GT integrations. The final chapter explains the use of classical genetics in gene targeting and phenotype interpretation to create mutations and elucidate gene functions. Gene Targeting: A Practical Approach 2e will therefore be of great value to all researchers studying gene function.
Download or read book Insights in Extreme Microbiology 2021 written by Andreas Teske and published by Frontiers Media SA. This book was released on 2023-02-03 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Research Report Corps of Engineers U S Army Cold Regions Research and Engineering Laboratory written by and published by . This book was released on 1967 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Nucleic Acid Protocols Handbook written by Ralph Rapley and published by Springer Science & Business Media. This book was released on 2008-06-29 with total page 997 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."
Download or read book Plant Cold Hardiness and Freezing Stress written by P.H. Li and published by Elsevier. This book was released on 2012-12-02 with total page 716 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Cold Hardiness and Freezing Stress: Mechanisms and Crop Implications, Volume 2 contains the proceedings of an International Seminar on Plant Cold Hardiness, held at the Sapporo Educational and Cultural Hall, Sapporo, Japan on August 11-14, 1981. Organized into five parts, this book aims to update the fundamental phenomena of plant cold acclimation and freezing behavior, to examine the hypotheses and ideas important to plant cold hardiness research, and to review the application of research findings for improving the quality of life. This volume particularly addresses the cold acclimation, freezing, and other temperature-related stresses in plants. Strategies for improving freezing survival are also presented. This volume will make an additional, significant contribution to researchers involved in understanding and planning research strategies for plant cold hardiness and for attenuating crop losses by frosts and severe winters.
Download or read book Decoding brain function through genetics written by Noriyoshi Usui and published by Frontiers Media SA. This book was released on 2022-05-03 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Embryonic Stem Cells written by Elena Notarianni and published by Oxford University Press, USA. This book was released on 2006 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an integral approach to the history and current methods for manipulation of embryonic stem (ES) cells by recognised experts. ES cell research attracts a level of interest that is unprecedented for any area of cell biology. Notarianni and Evans have provided a must-have manual for scientists engaged in stem cell research and a textbook for graduate courses in stem cell biology.
